This investigation was supported by Public. Health Service grant EF from the National

Transcription

1 1044 NOTES APPL. MICROBIOL. lyzed with one membrane filter depended upon the treatment of the milk (i.e., 10 ml for raw or pasteurized; 1 ml for milk heated at 145 C for 8 see; 0.1 ml for autoclaved milk). Autoclaving destroyed th6 inhibitory quality of the milk and served as a control throughout. APC of survivors lose their validity whenever plates contain more than 0.01 ml of milk, whereas the membrane filter Statement of Ownership, Management and Circulation required by the Act of October 23, 1962; Section 4369, Title 39, United States Code. 1. Date of Filing: October 1, Title of Publication: Applied Microbiology. 3. Frequency of Issue: Bi-monthly. 4. Location of known Office of Publication: Mount Royal and Guilford Aves., Baltimore, Md Location of the Headquarters or General Business Offices of Publisher: 115 Huron View Blvd., Ann Arbor, Michigan Publisher: American Society for Microbiology, 115 Huron View Blvd., Ann Arbor, Michigan Editor: George M. Savage, 1210 Forest Drive, Kalamazoo, Mich Managing Editor: Robert A. Day, 1235 Wines Drive, Ann Arbor, Mich Owners: (If owned by a corporation, its name and address must be stated and also immediately thereunder the names and addresses of stockholders owning or holding 1 per cent or more of total amount of stock. If not owned by a corporation, the names and addresses of the individual owners must be given. If owned by a partnership or other unincorporated firm, its name and address, as well as that of each individual, must be given.) American Society for Microbiology, 115 Huron View Blvd., Ann Arbor, Mich Known bondholders, mortgagees and other security holders owning or holding 1 per cent or more of total amount of bonds, mortgages or other securities are: None. 9. Paragraphs 7 and 8 include, in cases where the stockholder or security holder appears upon the books of the company as trustee or in any other fiduciary relation, the name of the person or cortechnique permitted accurate analysis of the survivors in up ta 10 ml of milk. The membrane filter technique permitted the measurement of spore populations previously obscured by inhibitors. This investigation was supported by Public Health Service grant EF from the National Institutes of Health. poration for whom such trustee is acting, also the statements in the two paragraphs show the affiant's full knowledge and belief as to the circumstances and conditions under which stockholders and security holders who do not appear upon the books of the company as trustees, hold stock and securities in a capacity other than that of a bona fide owner. Names and addresses of individuals who are stockholders of a corporation which itself is a stockholder or holder of bonds, mortgages or other securities of the publishing corporation have been included in Paragraphs 7 and 8 when the interests of such individuals are equivalent to 1 per cent or more of the total amount of the stock or securities of the publishing corporation. (a)* 10. A. Total No. Copies Printed (Net Press Run) 5270 B. Paid Circulation 1. To term subscribers by mail, carrier delivery or by other means Sales through agents, news dealers or otherwise C. Free Distribution (including samples), by mail, carrier delivery or by other none means D. Total No. of Copies Distributed (b)t none * Average number of copies for each issue during preceding 12 months. t Single issue nearest to filing date. I certify that the statements made by me above are correct and complete. (Signed) Robert A. Day, Managing Editor.

2 APPLIED MICROBIOLOGY, Nov., 1965 Copyright ( 1965 American Society for Microbiology Vol. 13, No. 6 Printed in U.S.A. AUTHOR INDEX Adams, A. Paul, 762 Adams, Ralph, 625 Adye, J. C., 208 Albury, M. N., 227 Alexander, A. D., 595 Ambekar, G. R., 713 Ammann, Elizabeth C. B., 546 Anderson, A. W., 1030 Anderson, R. F., 393 Anderson, Robert I., 552 Anellis, A., 37, 397, 527 Angelotti, Robert, 140, 352 Appleman, Milo D., 289 Arthur, Robert M., 125 Avigad, G., 686 Ayres, J. C., 358 Bailey, E. G., 109, 115 Baldwin, R. L., 194 Bankole, R. 0., 673 Banwart, George J., 77, 650 Barton, N. D., 15 Bautista, M., 977 Beargie, R. A., 279 Becker, B., 236 Benarde, Melvin A., 103, 776 Bennett, E. 0., 570 Bennett, F. W., 725 Bennett, R. E., 738 Bennett, Reginald W., 181 Berberich, Norbert J., Jr., 614 Berkeley, William H., 830 Berkowitz, D., 37, 397 Bhat, J. V., 257 Bhatla, M. N., 345 Bladel, B. 0., 281, 743 Blasco, Richard J., 473 Block, S. S., 5 Bluhm, Leslie, 646 Board, R. G., 358 Bolles, Dorothy, 128 Boniece, William S., 248 Boyle, W. C., 218 Bracken, E. C., 279 Bram, B., 842 Branch, Arnold, 469 Browder, Henry P., 491 Buckwalter, Frank H., 505 Bullock, Graham L., 89 Busta, F. F., 851, 858, 1043 Canada, James C., 788 Cantoni, C., 631 Carlucci, A. F., 663 Carroll, B. J., 502 Casas-Campillo, C., 977 Casida, L. E., Jr., 327 Casman, Ezra P., 181 Cassell, E. Alan, 293 Chamberlain, Robert E., 232 Charney, W., 515 Cherry, William B., 605 Chibata, Ichiro, 618, 638, 680 Christensen, C. M., 653 Christiansen, Lee N., 1023 Clark, Carol W., 646 Cliver, Dean O., 387, 417 Considine, Judith M., 464 Corlett, D. A., Jr., 808, 818 Corstvet, R. E., 348 Cowart, Glenda S., 605 Cox, Claire B., 447 Cox, W. A., 956 Crabtree, K., 218 Crisley, F. D., 140 Cropp, C. Bruce, 212 Dack, G. M., 1014 Daoust, Donald R., 478 Davis, E. N., 267 Davis, J. L., 99 Dela Cruz, Amy S., 201 DeLorenzo, S., 515 Demain, Arnold L., 757 Deutsch, David R., 1040 Dhala, Salim, 432 Dias, F. F., 257 Dimmick, R. L., 846 Dixon, Glen J., 579 Doctor, V. M., 713 Dunlop, Stuart G., 831 Dyer, Denzel L., 833 Edmonds, Paul, 823 Edwards, J. L., Jr., 851, 858 Eklund, M. W., 985 El-Bisi, Hamed M., 478 Elliker, P. R., 1030 Elliott, R. Paul, 73 Ellison, D., 595 El-Shazly, K., 62 Eppley, Richard W., 833 Erickson, R. C., 738 Farber, Lionel, 625 Featherston, W. R.,

3 1046 AUTHOR INDEX APPL. MICROBIOL. Fellowes, 0. N., 496, 694, 1038 Fields, Michael J., 81 Foster, E. M., 1023 Frank, Hilmer A., 201 Friend, Milton, 128 Frommhagen, Laurence H., 895 Gabrielson, Mary O., 967 Gaffney, Peter E., 507 Gailiunas, Peter, 872 Galton, Mildred M., 81 Gardner, Roberta A., 564 Gaudy, A. F., Jr., 345 Gerber, N. N., 935 Gibbons, N. E., 288 Godding, Rogene M., 10 Goerner, Gordon L., 314 Gordon, D. F., Jr., 537 Gori, Gio B., 93, 909 Goulet, N. R., 70 Graikoski, J. T., 244 Granstrom, Marvin L., 776 Grecz, Nicholas, 37, 397, 527, 1014 Greenberg, Richard A., 281, 743 Grim, Catherine A., 457 Groninger, H., 985 Hahon, Nicholas, 865 Hall, H. H., 1000 Hall, Herbert E., 352 Halle, Sidney, 503 Halvorson, H. Orin, 373 Hamdy, M. K., 15 Harmon, L. G., 335, 1010 Harrington, Rube, Jr., 494 Harrison, Edward F., 212, 491 Harstad, J. Bruce, 899 Heiniger, Patricia K., 73 Held, H. R., 132 Hendlin, David, 757 Henis, Y., 437 Herzog, H. L., 515 Hess, George E., 781 Highsmith, Anita K., 34 Hill, L. R., 631 Hoadley, A. W., 575 Hollowell, C. A., 918 Hood, Donald W., 886 Horvath, Raymond, 103 Howe, Jean M., 650 Hoyt, H. H., 635 Hsiung, G. D., 967 Huber, D. A., 37 Hung, P. P., 216 Hungate, R. E., 62 landolo, John J., 646 Imada, Yukio, 1 Irgens, Roar L., 373 Ishikawa, Setsuko, 680 Israel, Bernard M., 776 Ito, Hiroshi, 680 Iyengar, M. R. S., 749, 755 Jackson, Marion, 757 Jackson, R. W., 1000 Jacob, Theodore A., 757 Jay, James M., 120 Jennings, J. C., 322 Jerome, Norge W., 157 Jones, R. H., 725 Junek, A. J., 322 Kadull, Paul J., 925 Kajihiro, Edwin S., 720 Kakimoto, Toshio, 638 Kaneshiro, Tsuneo, 939 Kaplan, Murray A., 505 Karlson, Alfred G., 494 Kato, Jyoji, 638 Kaufman, D. D., 443 Kaufmann, 0. W., 521 Kearney, P. C., 443 Keith, Elizabeth S., 308 Kellogg, Douglas S., Jr., 171 Kelly, L. E., 340 Kempe, L. L., 244 Kende, Meir, 1026 Kilton, Roger M., 543 Kluepfel, D., 600 Knaysi, Georges, 500 Kobayashi, Kaetsu, 1 Koft, Bernard W., 103 Koike, Hideo, 122 Kono, Haruyoshi, 882 Kopp, Rudolf, 950 Kordan, H. A., 825 Krabbenhoft, K. L., 762, 1030 Krause, Frank P., 160 Kushner, D. J., 288 Lagoda, A. A., 393 Lamanna, Carl, 460 Lancini, G. C., 600 Landi, S., 132 Lange, Willy, 160 Lannon, James H., 505 Leatherwood, J. M., 771 Lechevalier, H. A., 236, 935 Lechevalier, M. P., 236 Lee, J. S., 808, 818 Lerke, Peter, 625 Levine, S. I., 70 Litchfield, Carol D., 886 Litsky, Warren, 106, 478 Liu, 0. C., 70

4 VOL. 13, 1965 AUTHOR INDEX 1047 Loehr, Raymond C., 1005 Loken, K. I., 635 Long, Sterling K., 973 Longeri, Luis, 167 Lum, Norma A., 201 Luthi, H. R., 511 Lynch, Victoria H., 10, 546 McClung, L. S., 559 McConnell, S. J., 595 McCoy, Elizabeth, 218, 575 McCune, R. W., 22 McDaniel, L. E., 109, 115 McDivitt, Maxine E., 157 Majumdar, M. K., 190 Majumdar, S. K., 190 Mallette, M. F., 460, 464 Malligo, John E., 931 Margalith, P., 876 Marks, C. L., 216 Markus, Z., 686 Marshall, Robert S., 521, 559 Massey, Richard L., 798 Masson, G., 288 Mateles, R. I., 208 Mattoni, Rudolf H. T., 798 May, K. N., 340 Mayberry, W. R., 698 Meredith, W. E., 86 Merrifield, Larry S., 660, 766 Meseck, E., 515 Midura, T. F., 244 Miller, G., 686 Miller, V. Richard, 77 Milone, N. A., 244 Mirocha, C. J., 653 Misenheimer, T. J., 393 Miyauchi, D., 985 Morihara, Kazuyuki, 793 Moyer, J. C., 511 Mukherjee, S. K., 227 Muller, Johannes, 950 Munson, Polly S., 831 Muraschi, Thelma F., 128 Murawski, A., 515 Nagatsu, Chizuko, 669 Nelson, G. H., 653 Nelson, John D., 801 Nutini, Leo G., 614 Olivieri, Vincent P., 776 Olson, B. H., 314, 322 Ordal, Z. John, 646 Packett, L. V., 22 Palmquist, D. L., 194 Parikh, G. C., 122 Parker, R. B., 1042 Patel, K. L., 749 Pathak, S. G., 262 Patrick, Roger, 973 Patrick, W. C., III, 99 Payne, W. J., 698, 702 Pederson, C. S., 227 Peeler, J. T., 140 Phillips, C. Alan, 457 Phillips, James W., 460 Pittman, Margaret, 447, 564 Pivnick, Hilliard, 365 Pollock, Marlene, 945 Poonawalla, F. M., 749, 755 Power, Edna E., 469 Powers, John J., 308 Pridham, Thomas G., 43, 1000 Prochazka, G. J., 702 Proteau, P. R., 838 Ray, John G., Jr., 297, 301, 305, 925 Rhodes, R. A., 267, 272 Richter, Alan, 503 Rigg, J., 595 Riley, H. D., Jr., 279 Riley, W. H., 28, 945 Robbins, Mary Louise, 1026 Roga, V., 322 Rohlich, G. A., 218 Rose, Robert E., 106 Rosemberg, J. A., 991 Rosett, Theodore, 254 Rosselet, J. P., 515 Sadler, W. W., 348 Sano, Ryujiro, 618 Santa Rosa, C. A., 81 Sartori, G., 600 Schabel, Frank M., Jr., 579 Schipper, I. A., 762 Schmidt, E. L., 673 Schmitt, J. A., Jr., 290 Schneider, M. D., 37 Schneierson, S. Stanley, 286 Schuurmans, D. M., 322 Schwarz, Y., 876 Schwegler, Diann T., 1005 Seligmann, Edward B., Jr., 830 Sellers, Sara M., 579 Seshadri, R., 262 Shaulis, Louis, 103 Shay, Donald E., 297, 301, 305, 706 Shechmeister, I. L., 122 Shelton, Sharon, 801 Shirato, Shiroh, 669 Shore, Bella, 286 Shotts, Emmett B., 34 Shulke, H. Russell, 267, 272

5 1048 AUTHOR INDEX APPL. MICROBIOL. Siddique, I. H., 635 Sidwell, Robert W., 579 Siepmann, R., 827 Silbernagel, S. B., 663 Silvestri, L. G., 631 Simon, M., 37 Sinnhuber, R. O., 808, 818 Smith, Rodney F., 706 Smuckler, Stan A., 289 Snyder, 0. P., 527 Solomons, G. L., 847 Speck, M. L., 537, 851, 858, 1043 Spinelli, J., 985 Stadelman, W.AJ., 650 Starkey, D. H., 469 Steenbergen, J. Frank, 559 Steinkraus, K. H., 227 Stoyla, Brigitta, 511 Strong, Dorothy H., 788 Stuart, Philip F., 365 Stutzenberger, F. J., 570 Sudman, M. S., 290 Sulzer, Catherine R., 81 Sundar Raj, C. V., 432 Taber, W. A., 590, 827 Tagari, H., 437 Takahashi, Joji, 1 Takao, Shoichi, 732 Tamir, Musha, 437 Tardrew, P. L., 216 Tertzakian, G., 590 Thadani, S. B., 713 Theriault, Robert J., 402 Thomason, Berenice M., 605 Tosa, Tetsuya, 618 Toussaint, Andre J., 552 Traxler, R. N., 838 Traxler, R. W., 838 Tyler, D. D., 393 Ueda, Seinosuka, 882 Vadehra, D. V., 335, 1010 Valu, Joseph A., 486 Van Veen, A. G., 227 Vitali, Ronald A., 757 Volcani, R., 437 Wagenaar, R. O., 1014 Wahba, A. H., 291 Walker, A. A., 527 Wallace, D. L., 1010 Wallen, L. L., 272 Wang, Wen Lan L., 831 Ward, B. Q., 502 Wasserman, Aaron E., 175 Wasserman, Felix E., 1040 Weiser, H. H., 86 Wessman, G. E., 426 White, Lendell A., 171 Wick, Warren E., 248 Williams, Joy P., 698 Winter, A. R., 86 Wolf, Paul A., 28, 945 Wolin, M. J., 918 Yager, R. H., 595 Yamada, Koichi, 1 Yamada, Shigeki, 680 Yang, H. Y., 660, 766 Yeatman, John, 387 Yokoya, Fumio, 993 York, George K., 993 Zemelman, Radll, 167 Zimmerli, A., 109, 115 Zingelmann, W. J., 743 Zweig, Gunter, 939 Zygmunt, Walter A., 491

6 APPLIED MICROBIOLOGY, Nov., 1965 Copyright 1965 American Society for Microbiology Vol. 13, No. 6 Printed in U.S.A. ABBOTT 29119, new antibiotic, 216 acids, organic, determination in fermentation media, 22 actinomycetes, cell-wall preparations from, 236 actinomycetes, earthy-smelling substance from, 935 aerosols, bacteriophage, sampling of, 899 aflatoxins, production in submerged cultures, 208 agar overlay method for recognition of enteroviruses, 967 agar-gel cutter, 122 agar-gel precipitin-inhibition technique, 297, 301, 305 agar-gel precipitin-inhibition technique for plaque antibody determinations, 925 agglutination test for leptospiral antibodies, 81 L-alanine, production by Pseudomonas dacunhae, 638 algae, continuous culture of, 833 algae, gas exchange of, 546 algae, mass assays of physiological characteristics of, 798 algae, mass culture of, 473 alpha sarcin, new antitumor agent, 314, 322 amino acid, new, sarcinine, 314 amino acid composition of cell-wall proteins, 650 L-amino acids, production by bacteria, 618 anaerobes, pipette count method for, 1042 animal passage, effect on Clostridium perfringens, 788 antibacterial heterocyclic quaternary ammonium compounds, site of action of, 956 antibiotic, new, from Streptomyces erythreus, 216 antibiotic sensitivity of microorganisms, tube dilution test for, 469 antibiotic sensitivity of organisms from mammary secretions, 762 antibiotic sensitivity of staphylococci in milk, 725 antibiotic susceptibility, determination of, 279 antibiotics, effect on leptospires in tissue culture, 595 antibiotics, new, cephaloglycin, 248 antibiotics, new, cephaloridine, 248 antibiotics from Micromonospora, 515 antibiotics from Physarum gyrosum, 464 antibodies, leptospiral, agglutination test for, 81 antigens, viral, purification of, 895 antimicrobial activity of bis-methanethiolsulfonates, 945 antimicrobial activity of vitamin K5, 766 antimicrobial effect of xanthines, 432 antitumor agent, new, alpha sarcin, 314, 322 SUBJECT INDEX antiviral activity of 1,3-bis (2-chloroethyl)-1- nitrosourea, 579 Aspergillus flavus, aflatoxin production by, 208 Aspergillus flavus, in soil, 673 Aspergillus niger, enzyme production by, 842 asphalt, effect of bacteria on, 838 asphalt, fungistats in, 28 Bacillus, germination temperatures of, 500 Bacillus lentimorbus, effect of antimicrobial agents on, 1000 Bacillus polymyxa, production of 2,3-butylene glycol from citrus wastes, 973 Bacillus popilliae, effect of antimicrobial agents on, 1000 Bacillus stearothermophilus, enumeration of, 1043 Bacillus subtilis spores, thermal inactivation of, 851, 858 Bacillus subtilis spores, viability in rocket propellants, 10 bacon, radiation sterilization of, 37 bacteria, L-amino acid production by, 618 bacterial disintegration, 1010 bactericidal activity of chlorine dioxide, 776 bacteriophage, growth in Shigella flexneri, 831 bacteriophage, streptococcal, destruction kinetics of, 478 bacteriophage aerosols, sampling of, 899 bacteriophage detection, filtration method for, 1005 bacteriophage 80/81 strains of Staphylococcus aureus, action of lysostaphin against, 491 bacteriophage 80/81 strains of Staphylococcus aureus, action of penicillins against, 491 bacteriophage types of staphylococci in milk, 725 bacteriophage typing, determination of coagulase activity during, 34 bacteriophages in activated sludge, 257 barbital metabolism by Streptomyces mediterranei, 600 basidiomycetes, organic acid production by, 732 bat guano, dermatophytes in, 720 Bdellovibrio, in activated sludge, 257 Betabacterium, deoxyribonucleic acid composition of, 631 bile salts, effect on microorganisms, 77 biodegradability of organic compounds, 702 biotin in seawater, 886 1,3-bis(2-chloroethyl)-l-nitrosourea, in vivo antiviral activity of, 579 bis-methanethiolsulfonates, antimicrobial activity of, 945 brilliant green, effect on microorganisms, 77 bruised tissue, Staphylococcus aureus in,

7 SUBJEC' jr I., _ssr- INDEX APPL. MICROBIOL. 2,3-butylene glycol, production from citrus wastes, 973 C-reactive protein, determination of, 297, 301, 305 calcium gluconate, production by Penicillium chrysogenum, 713 Candida, method for identification of, 286 carbohydrates, effect on protease production, 669 carbohydrates, effect on streptomycin production, 669 carbon dioxide, effect on glucose catabolism, 507 carob pod extract, effects in artificial rumen, 437 casein hydrolysate medium for Pasteurella hemolytica, 426 catabolism, effect of carbon dioxide on, 507 cell culture, rack for use in, 503 cell disruption with a peristaltic pump, 460 cellulase from rumen microorganisms, 771 cell-wall preparation from actinomyctes, 236 cell-wall proteins, amino acid composition of, 650 cephaloglycin, laboratory evaluation of, 248 cephaloridine, laboratory evaluation of, 248 cheese, Clostridium botulinum in, 1014 chicken meat, bacteria in, 340 Chlorella pyrenoidosa, gas exchange in, 546 Chlorella pyrenoidosa, heterotrophic growth of, 402 Chlorella pyrenoidosa, mass culture of, 473 Chlorella pyrenoidosa, xanthophyll production by, 402 chlorine dioxide, bactericidal activity of, chloroethyl-N-3-chlorophenylcarbamate, microbial degradation of, 443 chloroform, sensitivity of viruses to, 1040 chlortetracycline, in poultry tissues and eggs, 86 chromatography, gas-liquid, for determination of organic acids in fermentation media, 22 citrus wastes, production of 2,3-butylene glycol from, 973 clostridia, method for isolation and enumeration of, 281 Clostridium botulinum, development in milk, 521 Clostridium botulinum, growth in cured meats, 1023 Clostridium botulinum, in processed cheese, 1014 Clostridium botulinum, radiation killing of, 37 Clostridium botulinum spores, in meat, 743 Clostridium botulinum spores, radiation resistance of, 527 Clostridium botulinum spores, survival of, 397 Clostridium botulinum type E, presence in water, 502 Clostridium perfringens, heat resistance of, 788 Clostridium perfringens, in meat, 352 Clostridium perfringens, radiation resistance of, 244 Clostridium perfringens, technique for enumeration of, 559 coagulase activity of staphylococci, 34 coffee cherries, decomposition in, 201 color systems for actinomycetes, 43 composts as media for mushroom production, 5 computer analysis for identification of bacteria in foods, 808, 818 continuous cultivation of viruses, 909 continuous culture of algae, 833 corn steep liquor, effect on L-glutamic acid fermentation by hydrocarbons, 1 Corynebacterium, fermentative production of L- glutamic acid by, 1 coryneform bacterium, nucleotide production by, 757 counting bacterial colonies, device for, 931 counting data, method for estimating precision of, 293 Coxiella burnetii, purification of, 99 crab meat, yeasts from, 985 Dactylium dendroides, production of D-galactose oxidase by, 686 deoxyribonucleic acid, of Betabacterium, 631 deoxyribonucleic acid, of Streptobacterium, 631 dermatophytes, occurrence in bat guano, 720 detergents, effect on Proteus, 950 device for counting bacterial colonies, 931 dialysis fermentor for mammalian cell culture, 93 diet, effect on Staphylococcus aureus virulence in mice, 614 diquat, effect on Rhodospirillum rubrum, 939 disintegration of bacterial cells, 1010 echovirus 7, purification of, 895 egg yolk coloring material, production of, 876 eggs, antibiotic residues in, 86 eggs, bacterial infection of, 358 endospores, thermal death rate, 993 enterotoxin, detection in foods, 181 enteroviruses, concentration and detection of, 387 enteroviruses, membrane filtration of, 417 enteroviruses, recognition by agar overlay method, 967 enzymes, activity in rumen microorganisms, 194 epizootic, capsulated Pseudomonas from, 89 Erwinia-like microorganisms, 128 Escherichia coli, absence from rumen ecosystem, 918 Escherichia coli, effect of oxygen supply rate on growth of, 109, 115 Escherichia coli, sensitivity to mercurials, 570 estrogens, hydroxylation by Fusarium moniliforme, 977 everninomicin B, isolation from Micromonospora, 515 everninomicin D, isolation from Micromonospora, 515 fecal specimens, detection of shigellae in, 605 feces, isolation of salmonellae from, 365

8 VOL. 13, 1965 SUBJECT INDEX 1051 fermentation media, steam-volatile organic acids in, 22 fermentation of hydrocarbons by microorganisms, 1 fermentation of rumen microorganisms, 62 fermentor, dialysis, mammalian cell culture in, 93 fibrinogen-polymyxin medium for detection of staphylococci, 157 filtration method for bacteriophage detection, 1005 fish, identification of bacteria in, 818 fish muscle spoilage, bacteriology of, 625 Flavobacterium species, properties of, 801 flax, bacteria responsible for retting of, 991 flor sherry, continuous production from wines, 511 fluorescent cell-counting technique for virus assay, 865 fluorescent-antibody assay for simian virus-40, 70 fluorescent-antibody counterstain method for detection of Neisseria gonorrhoeae, 171 fluorescent-antibody technique, soluble antigen in, 552 fluorescent-antibody technique for observation of soil fungi, 673 food, Penicillium chrysogenum mycelium as, 262 food-poisoning, enterotoxin in, 181 foods, detection of staphylococci in, 140, 157 foods, detection of viruses in, 387 foods, rapid identification of bacteria in, 808, 818 foot-and-mouth disease virus, detection of, 872 foot-and-mouth disease virus, effect of surfaceactive agents on, 694 foot-and-mouth disease virus, storage stability of, 494 foot-and-mouth disease virus antiserum, activity after 0-propiolactone treatment, 1038 freeze-dehydration, effect on bacteria in chicken meat, 340 freeze-drying, direct-contact tray assembly for, 830 freeze-drying for storage of viruses, 496 fuel-water systems, evaluation of microbial inhibitors in, 823 fungi, invertase production by, 749 fungi, production of exocellular glucans by, 267, 272 fungi, production of D-galactose oxidase by, 686 fungi, soil, specificity of immunofluorescent staining for, 673 fungi, yeastlike, method for identification of, 286 fungistatic action of 10,10'-oxybisphenoxarsine in paints, 28 fungistatic agent, vitamin K5 as, 660 Fusarium, growth in soils, 160 Fusarium, toxic substance from, 653 Fusarium moniliforme, hydroxylation of estrogens by, 977 D-galactose oxidase, production by Dactylium dendroides, 686 garbage composting for mushroom production, 5 gas exchange of algae, 546 gel-cutting device, 122 genetic strain, effect on Staphylococcus aureus virulence in mice, 614 geosmin, earthy-smelling substance from actinomycetes, 935 glucans, production by fleshy fungi, 267, 272 L-glutamic acid, fermentative production from hydrocarbons, 1 glycerol, effect on growth of bacteriophage in Shigella flexneri, 831 goldfish, Pseudomonas from, 89 gram-negative bacteria from surface waters, 575 halophilic bacteria, simple method for killing, 288 heat resistance of Clostridium perfringens, 788 heat resistance of Salmonella, 73 Herellea species, properties of, 801 hydrocarbons, L-glutamic acid fermentation of, 1 idli, fermentation of the batter of, 227 immunofluorescence, detection of shigellae by, 605 incubator, temperature-gradient, improved, 73 incubator for microbial growth, 846 inosine-5'-monophosphate, production by coryneform bacterium, 757 invertase production by Penicillium chrysogenum, 749 isoleucine stereoisomers, use by Lactobacillus fermenti, 680 isopropyl-n-3-chlorophenylcarbamate, microbial degradation of, ketogluconic acid, production by Serratia marcescens, 393 kidney tissue cultures, antibiotic effects on leptospires in, 595 lactic acid bacteria, leavening action of, 227 lactic streptococcal bacteriophage, destruction kinetics of, 478 Lactobacillus fermenti, use of 4soleucine stereoisomers by, 680 Lactobacillus leichmannii, survival in relation to B12 assays, 486 leavening action of lactic acid bacteria, 227 leptospiral antibodies, detection in sera, 81 leptospires in canine kidney tissue cultures, 595 Leuconostoc mesenteroides, use as leavening agent,; 227 lipases of Staphylococcus aureus, action on milk fat, 335 lymphocytic choriomeningitis virus, activity of 1,3-bis(2-chloroethyl)-1-nitrosourea against, 579

9 1052 SUBJECT INDEX APPL. MICROB3IOL. lysostaphin, activity against Staphylococcus aureus, 212, 491 lysostat, use in virus cultivation, 909 mammalian cells, concentrated cultures of, 93 mammary secretions, antibiotic sensitivity of organisms from, 762 mass culture of Chlorella pyrenoidosa, 473 meat, Clostridium botulinum spores in, 743 meat, Clostridium perfringens in, 352 meat, Micrococcus radiodurans in, 1030 meats, effect of vacuum packaging on bacteria in, 1023 meats, relation of water-holding capacity to microbial quality, 120 media for coagulase-positive staphylococci, 140 medical microbiology, polarizing microscopy in, 543 medium used in rapid enumeration of Clostridium perfringens, 559 mercurials, sensitivity of mixed cultures to, 570 metabolism in Penicillium chrysogenum, 590 microbial isomerization of amino acids, 618 Micrococcus radiodurans, ecology of, 1030 Micromonospora, chemistry of antibiotics from, 515 microscopy, polarizing, in medical microbiology, 543 milk, assay for neomycin in, 635 milk, Bacillus stearothermophilus in, 1043 milk, destruction of mycobacteria in, 494 milk, development of Clostridium botulinum in, 521 milk, staphylococci in, 157, 167, 725 milk cultures, bitter peptide from Streptococcus cremoris in, 537 milk fat, action of Staphylococcus aureus lipases on, 335 milky disease bacteria, effect of antimicrobial agents on, 1000 Mima species, properties of, 801 minerals, effect on neomycin production, 190 mold growth in soils, 160 molds, growth in liquid cultures, 827 mushrooms, production on garbage compost, 5 mutants, coryneform bacterium, nucleotide production by, 757 mutants, Serratia marinorubra, biochemically deficient, 663 mycelium, Penicillium chrysogenum, use as food, 262 mycobacteria, destruction in milk, 494 Mycobacterium ranae, effect on asphalt, 838 Mycobacterium tuberculosis, purified protein derivative from, 132 naringinase, production by Aspergillus niger, 842 Neisseria gonorrhoeae, detection of, 171 neomycin, effect of minerals on production, 190 neomycin in milk, assay of, 635 Nocardia coeliaca, effect on asphalt, 838 organic acid production by basidiomycetes, 732 organic compounds, biodegradability of, 702 oscillator, sonic, cooling device for, ,10'-oxybisphenoxarsine, fungistatic action in paints, 28 oxygen, effect on aerosolized Serratia marcescens, 781 oxygen consumption, device for recording, 125 oxygen supply rates, effect on Escherichia coli growth, 109, 115 oxygen uptake, effect of carbon dioxide on, 507 oxygen uptake by heterogeneous microbial populations, 345 oxytetracycline, in poultry tissues and eggs, 86 paints, fungistats in, 28 paper wastes, use for mushroom production, 5 Pasteurella hemolytica, cultivation of, 426 Pasteurella pestis, assay of antibodies for, 925 Pasteurella tularensis, growth of vaccine strain of, 232 pasteurization, destruction of mycobacteria in milk by, 494 penicillin acylase activity of Penicillium chrysogenum, 738 penicillins, activity against Staphylococcus aureus, 491 Penicillium chrysogenum, calcium gluconate production by, 713 Penicillium chrysogenum, invertase production by, 749 Penicillium chrysogenum, metabolism in, 590 Penicillium chrysogenum, penicillin acylase activity of, 738 Penicillium chrysogenum mycelium, use as food, 262 peptide, in milk cultures of Streptococcus cremoris, 537 peristaltic pump, grinding microorganisms with, 460 pertussis vaccine, stability and potency of, 564 pertussis vaccine testing, 447 pesticides, microbial degradation of, 443 phage, see bacteriophage phenolic acids, effect on bacteria in urine, 308 photosynthetic growth of Rhodospirillum rubrum, 939 Physarum gyrosum, antibiotics from, 464 picornaviruses, comparison of elution and adsorption, 457 pigment production for differentiation of Trichophyton species, 290 pigments produced by Pseudomonas, 175

10 VOL. 13, 1965 SUBJECT INDEX 1053 pipette ount method for obligate anaerobes, 1042 plague antibody determinations, 925 plant nutrients, removal from sludge, 373 plant pathogen, isolation from animal hosts, 128 polarizing microscopy in medical microbiology, 543 poliovirus, titration in micro tissue culture, 1026 poliovirus I, purification of, 895 poly-j8-hydroxybutyrate, identity of sudanophilic granules as, 218 polymer formation by fleshy fungi, 267, 272 polysaccharide produced by Pullularia, 882 poultry, Salmonella infection of, 348 poultry, Staphylococcus aureus in bruised tissue of, 15 poultry tissues, antibiotic residues in, 86,-propiolactone, use to inactivate contaminating virus, propiolactone decontamination of simian virus- 40, 70 proteases, extraction from bacteria, 1010 proteases, production by Streptomyces griseus, 669 protein, C-reactive, determination of, 297, 301, 305 proteinase, production by Pseudomonas aeruginosa, 793 proteins, cell-wall, amino acid composition of, 650 Proteus, effect of detergents on, 950 protoplasts, steroid lysis of, 706 pseudomonads in lake and stream waters, 575 Pseudomonas, capsulated, from goldfish, 89 Pseudomonas, degradation of organic compounds by, 702 Pseudomonas, pigments produced by, 175 Pseudomonas, primary alcohol sulfatase in, 698 Pseudomonas aeruginosa, proteinase production by, 793 Pseudomonas aeruginosa, pyrorubrin-producing, 291 Pseudomonas dacunhae, production of L-alanine by, 638 Pullularia, polysaccharide produced by, 882 purification of Coxiella burnetii, 99 pyrorubrin production by Pseudomonas aeruginosa, 291 quarternary ammonium compounds, site of action of, 956 rack for use in cell culture, 503 radiation, effect on Clostridium botulinum spores, 397 radiation injury of Clostridium botulinum spores, 743 radiation resistance of Clostridium botulinum spores, 527 radiation resistance of Clostridium perfringens spores, 244 radiation sterilization of bacon, 37 replica plating for identification of bacteria in foods, 808, 818 respirometer, automatically recording, 125 Rhodospirillum rubrum, effect of diquat on, 939 Rhodotorula mucilaginosa, effect on egg yolk color, 876 rifamycin B, effect of Streptomyces mediterranei metabolic products on, 600 rocket propellants, Bacillus subtilis spores in, 10 rumen ecosystem, absence of Escherichia coli from, 918 rumen microorganisms, cellulase from, 771 rumen microorganisms, effect of carob extract on, 437 rumen microorganisms, enzyme activity in, 194 rumen microorganisms, fermentation of, 62 Ruminococcus albus, cellulase from, 771 Salmonella, heat resistance of, 73 Salmonella, maximal growth temperature of, 73 Salmonella infection of market poultry, 348 salmonellae, effect of bile salts on, 77 salmonellae, effect of brilliant green on, 77 salmonellae, isolation by selective motility system, 365 sarcinine, new amino acid, 314 seawater, microbiological assay for organic compounds in, 886 seawaters, effect on development of Serratia marinorubra mutants, 663 sera, leptospiral antibodies in, 81 Serratia marcescens, effect of oxygen on, 781 Serratia marcescens, growth in thermal gradient incubator, 846 Serratia marcescens, production of 2-ketogluconic acid by, 393 Serratia marinorubra, in assay for organic compounds in seawater, 886 Serratia marinorubra mutants, biochemically deficient, 663 serum, determination of C-reactive protein in, 297, 301, 305 sewage, microbial populations in, 345 sewage, removal of plant nutrients from, 373 sewage, survival of Shigella in, 831 sewage sludge, use for mushroom production, 5 Shigella, survival in sewage, 831 shigellae, detection by immunofluorescence, 605 simian virus-40,,-propiolactone decontamination of, 70 slime molds, antibiotics from, 464 sludge, activated, microbial ecology of, 257 sludge, activated, removal of plant nutrients from, 373 sludge, activated, Zooglea ramigera in, 218

11 1054 SUBJECT INDEX APPL. MICROIBIOL. sodium borohydride, inactivation with tetracycline, 505 soil fungi, specificity of immunofluorescent staining for, 673 soil microorganisms, isolation of, 327 soils, mold growth in, 160 soluble antigen fluorescent-antibody technique, 552 sonic oscillator, cooling device for, 254 spacecraft, sterility of propellants for, 10 spoilage of fish muscle, bacteriology of, 625 spoilage of meats, relation to water-holding capacity, 120 spores, Bacillus subtilis, thermal inactivation of, 851, 858 spores, Bacillus subtilis, viability in rocket propellants, 10 spores, Clostridium botulinum, in meat, 743 spores, Clostridium botulinum, radiation resistance of, 527 spores, Clostridium botulinum, survival of, 397 spores, Clostridium perfringens, radiation resistance of, 244 staphylococcal enterotoxin, detection in foods, 181 staphylococci, coagulase activity of, 34 staphylococci, detection in milk, 157, 167 staphylococci, media for detection of, 140 staphylocci in milk, 725 Staphylococcus aureus, action of lysostaphin against, 491 Staphylococcus aureus, action of penicillin against, 491 Staphylococcus aureus, antibiotic action on, 212 Staphylococcus aureus, factors affecting virulence in mice, 614 Staphylococcus aureus, fate in bruised tissue, 15 Staphylococcus aureus, growth in cured meats, 1023 Staphylococcus aureus, repression in associative culture, 646 Staphylococcus aureus, sensitivity to mercurials, 570 Staphylococcus aureus lipases, action on milk fat, 335 Staphylococcus Medium No. 110, improved, 289 sterilization of bacon by radiation, 37 steroids, in lysis of protoplasts, 706 Streptobacterium, deoxyribonucleic acid composition of, 631 streptococcal bacteriophage, destruction kinetics of, 478 streptococci, fecal, isolation from water, 106 Streptococcus cremoris, bitter peptide from cultures of, 537 Streptomyces erythreus, new antibiotic from, 216 Streptomyces fradiae, neomycin production by, 190 Streptomyces griseus, earthy-smelling substance from, 935 Streptomyces griseus, fermentation by, 669 Streptomyces mediterranei, barbital metabolism by, 600 streptomycetes, color of, 43 streptomycin, production by Streptomyces griseus, 669 submerged culture, calcium gluconate production in, 713 submerged culture, production of aflatoxins in, 208 submerged fermentation, invertase production in, 749 sudanophilic granules of Zooglea ramigera, 218 sulfatase, primary alcohol, in Pseudomonas, 698 surface-active agents, effect on viruses, 694 surface water bacteria, 575 swine, effect of Fusarium-infected feed on, 653 Ti bacteriophage aerosols, sampling of, 899 temperature, effect on bacterial infection of eggs, 358 tetracycline, inactivation with sodium borohydride, 505 tetracycline, vitamin B12 assay in prescence of, 755 thermal death rate of bacterial endospores, 993 thermal destruction kinetics of streptococcal bacteriophage, 478 thermal gradient incubator for microbial growth, 846 thermal inactivation of Bacillus subtilis spores, 851, 858 thiamine, effect on L-glutamic acid fermentation by hydrocarbons, 1 tissue culture technique for virus titration, 1026 tissue culture vessel for gas exchange, 825 tissue cultures for virus detection, 872 Trichophyton mentagrophytes, pigment production by, 290 Trichophyton rubrum, pigment production by, 290 tube dilution test for antibiotic sensitivity of microorganisms, 469 tuberculin purified protein derivative, 132 tularemia vaccine, live, 232 urine, effect of phenolic acids on bacteria in, 308 uterus, effect of toxic substance from Fusarium on, 653 vaccine, pertussis, testing of, 447 vaccine, tularemia, live, 232 vaccines, pertussis, stability and potency of, 564 vacuum packaging of cured meats, effect on bacterial growth, 1023 variola virus, assay of, 865

12 VOL. 13, 1965 SUBJECT INDEX 1055 viral antigens, purification of, 895 virus, cultivation in the lysostat, 909 virus, foot-and-mouth disease, detection of, 872 virus, lymphocytic choriomeningitis, activity of 1,3-bis (2-chloroethyl)-1-nitrosourea against, 579 virus, polio, titration in micro tissue culture, 1026 virus, simian virus-40,,b-propiolactone decontamination of, 70 virus, storage stability of, 496 virus, variola, assay of, 865 virus contamination of antisera, 1038 viruses, comparison of elution and adsorption, 457 viruses, detection in foods, 387 viruses, effect of surface-active agents on, 694 viruses, membrane filtration of, 417 viruses, recognition by agar overlay method, 967 viruses, sensitivity to chloroform, 1040 vitamin B12, assay in presence of tetracycline, 755 vitamin B12 assays, survival of Lactobacillus leichmannii in relation to, 486 vitamin K5, antimicrobial activity of, 766 vitamin K5 as a fungistatic agent, 660 waste water, microbial populations in, 345 waste water, treatment with chlorine dioxide, 776 waste water, Zooglea ramingera in, 218 water, Micrococcus radiodurans in, 1030 water, polluted, isolation of streptococci from, 106 water, Pseudomonas in, 575 water, treatment with chlorine dioxide, 776 wines, production of flor sherry from, 511 xanthines, effect on bacteria, 432 xanthophylls, production by Chlorella pyrenoidosa, 402 xanthosine-5'-monophosphate, production by coryneform bacterium, 757 yeast, effect on egg yolk color, 876 yeasts, from Pacific crab meat, 985 Zooglea ramigera, sudanophilic granules of, 218 Month January March May July September November INDEX TO DATE OF ISSUE Date of Issue 1/19/65 4/ 1/65 5/27/65 7/10/65 9/14/65 11/15/65 Pages