Product Datasheet. Cytokeratin 8 Antibody (OTI1B12) NBP Unit Size: 0.1 ml. Store at -20C. Avoid freeze-thaw cycles.

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1 Product Datasheet Cytokeratin 8 Antibody (OTI1B12) NBP Unit Size: 0.1 ml Store at -20C. Avoid freeze-thaw cycles. Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 9/20/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NBP Cytokeratin 8 Antibody (OTI1B12) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity Buffer Target Molecular Weight Product Description Host 0.1 ml Please see the vial label for concentration. If unlisted please contact technical services. Store at -20C. Avoid freeze-thaw cycles. Monoclonal OTI1B % Sodium Azide IgG2b Immunogen affinity purified PBS (ph 7.3), 1.0% BSA and 50% Glycerol 53.5 kda Mouse Gene ID 3856 Gene Symbol Species Marker Specificity/Sensitivity Immunogen Product Application Details Applications KRT8 Human, Rat, Canine, Monkey Epithelial Cell Marker This antibody is specific for Homo sapiens keratin 8 (KRT8). Recombinant protein fragment corresponding to amino acids of human Cytokeratin 8 (NP_002264) produced in E.coli. Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin Recommended Dilutions Western Blot 1: , Immunohistochemistry 1:50, Immunocytochemistry/Immunofluorescence 1:50-100, Immunohistochemistry- Paraffin 1:50 Images Western Blot: Cytokeratin 8 Antibody (1B12) [NBP ] Analysis of extracts (35ug) from 9 different cell lines by using anti-cytokeratin 8 monoclonal antibody. Page 1 of 5 v.20.1 Updated 9/20/2018

3 Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (1B12) [NBP ] - Staining of COS7 cells transiently transfected by pcmv6-entry Cytokeratin 8. Page 2 of 5 v.20.1 Updated 9/20/2018 [NBP ] - Staining of paraffin-embedded Human bladder tissue within the normal limits using anti-krt8 mouse monoclonal antibody at 1:150 dilution. Western Blot: Cytokeratin 8 Antibody (1B12) [NBP ] - HEK293T cells were transfected with the pcmv6-entry control (Left lane) or pcmv6-entry Cytokeratin 8 (Right lane) cdna for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-cytokeratin 8. Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (1B12) [NBP ] - Immunofluorescent staining of A549 cells using anti-cytokeratin 8 mouse monoclonal antibody.

4 Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (1B12) [NBP ] - Immunofluorescent staining of HeLa cells using anti-cytokeratin 8 mouse monoclonal antibody. Page 3 of 5 v.20.1 Updated 9/20/2018 [NBP ] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-krt8 mouse monoclonal antibody at 1:150 dilution. [NBP ] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-krt8 mouse monoclonal antibody at 1:150 dilution. [NBP ] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-krt8 mouse monoclonal antibody at 1:150 dilution.

5 [NBP ] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-krt8 mouse monoclonal antibody at 1:150 dilution. Page 4 of 5 v.20.1 Updated 9/20/2018 [NBP ] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-krt8 mouse monoclonal antibody at 1:150 dilution

6 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NBP NBP HAF007 NB720-B NBP Cytokeratin 8 Knockout HeLa Cell Lysate Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] Mouse, Human IgG2b Isotype Control (MPC-11) Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: