Product Data Sheet - TRUEMAB

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1 Name:CK8 (Keratin 8) mouse monoclonal antibody, clone OTI2E4 (formerly 2E4) Product Data Sheet - TRUEMAB Catalog: TA Components: CK8 (Keratin 8) mouse monoclonal antibody, clone OTI2E4 (formerly 2E4) (TA500022) WB positive control also available: 20ug myc-ddk tagged KRT8 HEK293T over-expression lysate lyophilized in RIPA buffer (LC419425). (Reconstitute into 20ul of 1x SDS sample buffer before loading; load 5ul per lane as WB control or as desired) Amount: 100ul Immunogen: Human recombinant protein fragment corresponding to amino acids of human CK8 (NP_002264) produced in E.coli. Host: Mouse Isotype: IgG1 Species Reactivity: Human Guaranteed Applications: WB, IHC, IF Suggested Dilutions: WB 1:500~1000, IHC 1:50, IF 1:50~100, Concentration: 1.46 mg/ml Buffer: PBS (ph 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. Purification: Purified from mouse ascites fluids by affinity chromatography Storage Condition: Shipped at -20C or with ice packs. Upon delivery store at -20C. Dilute in PBS (ph7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws. Target Target Name: Homo sapiens keratin 8, type II (KRT8), transcript variant 2 Alternative Name: Database Link: Function: CARD2; CK-8; CK8; CYK8; K2C8; K8; KO NP_ Entrez Gene 3856 Human Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity

2 and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

3 Validation Data HEK293T cells were transfected with the pcmv6- ENTRY control (Left lane) or pcmv6-entry KRT8 (RC209570, Right lane) cdna for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti- KRT8. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-krt8 monoclonal antibody. Adenocarcinoma of Human breast tissue using anti- KRT8 mouse monoclonal antibody.(heat-induced (ph8.5) at

4 Human Kidney tissue within the normal limits using anti- KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, ph6.0, 100C for 10min, TA500022) Carcinoma of Human liver tissue using anti-krt8 mouse monoclonal antibody.(heat-induced epitope Carcinoma of Human lung tissue using anti-krt8 mouse monoclonal antibody. (Heat-induced epitope

5 Human Ovary tissue within the normal limits using antikrt8 mouse monoclonal antibody. (Heat-induced (ph8.5) at 120 Cfor 3 min, TA500022) (1:750) Adenocarcinoma of Human ovary tissue using antikrt8 mouse monoclonal antibody.(heat-induced (ph8.5) at Human pancreas tissue within the normal limits using anti-krt8 mouse monoclonal antibody. (Heat-induced (ph8.5) at 2016 OriGene Technologies, Inc Medical Center Dr., Suite 200, Rockville, MD 20850

6 Carcinoma of Human thyroid tissue using anti-krt8 mouse monoclonal antibody. (Heat-induced epitope Human endometrium tissue within the normal limits using anti-krt8 mouse monoclonal antibody. (Heatinduced epitope retrieval by 1mM EDTA in 10mM Tris buffer (ph8.5) at Adenocarcinoma of Human endometrium tissue using anti-krt8 mouse monoclonal antibody. (Heat-induced (ph8.5) at

7 Human prostate tissue within the normal limits using anti-krt8 mouse monoclonal antibody.(heat-induced (ph8.5) at Carcinoma of Human prostate tissue using anti-krt8 mouse monoclonal antibody. (Heat-induced epitope Carcinoma of Human bladder tissue using anti-krt8 mouse monoclonal antibody.(heat-induced epitope

8 Anti-KRT8 mouse monoclonal antibody (TA500022) immunofluorescent staining of COS7 cells transiently transfected by pcmv6-entry KRT8(RC209570). Immunofluorescent staining of A549 cells using anti- KRT8 mouse monoclonal antibody (TA500022). * More validation images may be available on our website: