Schedule of Accreditation issued by United Kingdom Accreditation Service High Street, Feltham, Middlesex, TW13 4UN, UK

Transcription

1 21-47 High Street, Feltham, Middlesex, TW13 4UN, UK Lanwades Park Kentford Newmarket Suffolk CB8 7UU Contact: Sue Cade Tel: +44 (0) Fax: +44 (0) Website: Testing performed at the above address only FLUIDS DETAIL OF ACCREDITATION Biochemistry Blood Quantification of: AP ALT GGT Bile Acids Urea Creatinine Cholesterol Triglycerides Amylase Lipase AST CK Glucose Sodium Potassium Chloride Calcium Phosphate Total Protein Albumin Total Bilirubin Total Globulins A/G Ratio Documented In-house Method: SOP/CLIN/26 Labmedics Konelab 20i Blood - dogs, cats and horses Haematology Full Blood Count SOP/CLIN/29 ADVIA Haematology Analyser Assessment Manager: AS1 Page 1 of 5

2 Haematology (cont d) Blood, EDTA anti-coagulated, dogs cats and horses Blood, Li heparin anti-coagulated, dogs and horses Fresh and fixed tissues processed Full blood count including automated reticulocyte count Manual reticulocyte count Smear evaluation - white blood cell differential Histopathology structures and composition Identification of basophilic and eosinophilic structures Presence of Acid Alcohol Fast Bacilli (AAFB) Presence of Gram positive and Gram negative bacteria Identification of structures and cell morphologies: Collagen/smooth muscle (differentiation) Mucopolysaccharides, fungal hyphae, Glycogen SOP/CLIN/29 ADVIA haematology analyser SOP/CLIN/05, microscopy SOP/CLIN/03 using the Hema-tek automatic slide stainer (SOP/CLIN/04) Documented In-house Methods for staining and reading, supported by SOPs for cutting up, processing, embedding dehydration, clearing and mounting (SOP/PATH/15, SOP/PATH/10, SOP/PATH/09, SOP/PATH/04) Using Shandon or Histos 5 tissue processors SOP/PATH/11 Haematoxylin and Eosin ( H & E ) stains SOP/PATH/38 (Ziehl Neelsen) SOP/PATH/33 (Hucker Conn Gram) Documented In-house Methods using Specialist Stains as below: SOP/PATH/37 (Van Gieson) SOP/PATH/34 (Periodic Acid Schiff - PAS) Assessment Manager: AS1 Page 2 of 5

3 Fresh and fixed tissues processed Fresh and Fixed Tissues processed Swabs Nasal Swabs Serum Histopathology Continued structures and composition Non-haemoglobin iron structures and composition: Mast cells Microbiology Isolation and identification of Klebsiella pneumoniae & Pseudomonas aeruginosa Isolation and identification of Streptococcus equi Detection of antibodies to Babesia caballi (Piroplasmosis) Detection of antibodies to Theileria (Babesia) equi (Piroplasmosis) Detection of IgG antibodies to Streptococcus equi Documented In-house Methods for staining and reading, supported by SOPs for cutting up, processing, embedding dehydration, clearing and mounting (SOP/PATH/15, SOP/PATH/10, SOP/PATH/09, SOP/PATH/04 SOP /path/39) Using Shandon or Histos 5 tissue processors SOP/ PATH/35 (Perls Prussian Blue) SOP/PATH/40 (Toluidine Blue) SOP/ BACT/08 supported by SOP/BACT/01 - conventional culture technique using CLED and PCFC agars SOP/ BACT/02 supported by SOP/BACT/01 - conventional culture technique using COBA agar SOP/VIROL/49 by VMRD celisa software SOP/VIROL/49 by VMRD celisa software SOP/VIROL/44 - in house ELISA Software Assessment Manager: AS1 Page 3 of 5

4 Equine nasal swabs and tissues Equine nasopharyngeal swabs, tracheal wash and bronchialalveolar lavage and tissues Equine nasopharyngeal swabs, nasal and tracheal washes or aspirates, guttural pouch washes and chrondroids, swabs or pus from abscesses Equine and asinine genital and endometrial swabs Canine cheek swabs or whole blood (EDTA) Canine cheek swabs Molecular Biology Detection of Equine Influenza Virus RNA Detection of Equine Herpes Virus Types 1 and 4 Detection of Streptococcus equi DNA Detection of Taylorella equigenitalis and Taylorella Molecular Genetics Hereditary Cataract Test DNA Profiling Golden Retriever Progressive Retinal Atrophy (PRA1) SOP/VIROL/48 qpcr using ABI StepOnePlus thermal cycler SOP/VIROL/47 qpcr using ABI StepOnePlus thermal cycler or Techne Quantica SOP/BACT/36 qpcr using ABI StepOnePlus thermal cycler SOP/BACT/39 qpcr using ABI StepOnePlus thermal SOP/IMUN/70 Appendix V for PCR extraction from cheek swab or SOP/IMUN/49 - DNA extraction from blood) using the MJ Tetrad thermal cycler and capillary 3130xl genetic analyser SOP/IMUN/52 for PCR (supported by SOP/IMUN/43 - DNA extraction from cheek swab or SOP/IMUN/49 - DNA extraction from blood) using SOP/IMUN/70 Appendix XI for PCR Assessment Manager: AS1 Page 4 of 5

5 Molecular Genetics (cont d) Golden Retriever Progressive Retinal Atrophy (PRA) 2 Tibetan Spaniels and Tibetan Terriers Progressive Retinal Atrophy (PRA) 3 Primary Lens Luxation Test (PLL) SOP/IMUN/70 Appendix XI for PCR SOP/IMUN/70 Appendix XIII for PCR SOP/IMUN/69 Appendix 1 for PCR ABI StepOne Plus Real-Time PCR System Cattle Hair DNA Profiling SOP/IMUN/46 for PCR (supported by SOP/ IMUN/01 extraction of DNA from hair)) using the MJ Tetrad thermal cycler and capillary 3130xL genetic analyser Equine blood and hair DNA Profiling SOP GSERV/04 Multiplex PCR (supported by GSERV/02 extraction of DNA from blood or SOP/GSERV/03 extraction of DNA from hair) using Labtech International GS4M G-Storm thermal cycler and capillary 3130xL genetic analyser BACTERIAL CULTURES Molecular Biology Klebsiella pneumonia Capsule typing K1, K2 and K5 SOP/BACT/38 Multiplex PCR using MJ Tetrad Thermal Cycler and agarose gel electrophoresis END Assessment Manager: AS1 Page 5 of 5