Producing higher value products from aquatic biomass Visions and Technologies Lene Lange

Transcription

1 Producing higher value products from aquatic biomass Visions and Technologies Lene Lange Professor, PhD et Dr.Scient. Center for Bioprocess Engineering DTU, Technical University of Denmark

2 The Focus in Biorefinery is changing New trends: Diversity of Biomasses, both terrestrial and marine Large and Small scale biomass conversion; all year round! Cascading use of biomass, using both energy content and structure Focus on higher value products, feed additives, food ingredients, health, nutrition, skin & wound care Many aquatic bio-resources have exceptionel health potentials

3 Global challenges: Bioeconomy can deliver to the SDGs -Should be an integrated part of Climate Change/COP Feeding the world Getting more out of land and harvest Mitigating Climate Change Substitute fossils with renewables Job creation -Local, rural and coastal development Faster use of new knowledge! so many opportunities for People, Profit & Planet!

4 Background and Experience Cross disciplinary experience, Private sector & Academia 20 years, Private Industry R&D: Novo, Novo Nordisk and Novozymes (Research Director in Molecular Biotechnology & Microbial Enzymes) Last 10 years in Danish Universities (Research Director & Professor) Early career: Danida, Research collaboration, India, Africa, S.America Current Bioeconomy Advisory roles (seletected items): EU Commission: Vice Chair, Scientific Committee for BBI Bioeconomy Funding program (3.7 billion Euro) Nordic Council of Ministers, member of Nordic Bioeconomy Panel Danish Government, Member of Danish Bioeconomy Panel EU Expert Committee, Reviewing EU Bioeconomy Strategy, 2017 BIOTECH IAB / Advisory to the Thai Government Throughout my career: My own research group! Acknowledgement: Casper Wilkens, Y. Huang, Bo Pilgaard, Kristian Barrett & P.K.Busk 4 4 June 2018

5 DTU Chemical Engineering, Technical University of Denmark The Biomass Cascade Pyramide

6 Bioeconomy -many types of Biomass Conversion The Yellow Biorefinery (straw, corn stover, wood) The Green Biorefinery (fresh green biomass) The Blue Biorefinery (fish by-catch & cut offs; seaweeds) The Red Biorefinery (slaughterhouse residues) The White Biorefinery (forestry- & agroindustry side streams) The Brown Biorefinery (sludge & household waste) The Purple Biorefinery (CO2 and methane used as substrates) DTU Chemical Engineering, Technical University of Denmark

7 The Blue Bioeconomy Converting marine biomass, Algae, macro and micro Fish innards, cut offs and bycatch Mussels Invertebrates (sea cucumber and star fish) Sea Urchins Aquatic microbes (for bioprospecting) into higher value, health-promoting products 4 June

8 Blue Biorefinery: upgrade of marine biomass Seaweeds Fish, innards and cut offs Fish, by-catch Mussels Invertebrates (sea cucumber) Now: 95% of the cod is used; earlier only 50% 8 DTU Chemical Engineering, Technical University of Denmark 4 June 2018

9 Marine Biomass: Fish processing side streams Now: 95% of the cod is used; earlier only 50% Head, tail and backbone: Protein and Oil: food and feed ingredients (by enzymatic hydrolysis -BioMega) Fish Peptides, for health Human food Fish liver: (by exporting dried product to West Africa) Oil now also from herring and makrel Detoxed in France (heavy metal ions removed) Fish skin: Hydrolyzed collagen as health promoting product (Codland project) 9 DTU Chemical Engineering, Technical University of Denmark 4 June 2018

10 Marine biomasses (year round operations): Mussels, Invertebrates etc Mussels: new feed stock for the blue biorefinery Invertebrates: new feed stock for the blue biorefinery (sea cucumber; starfish) 10 DTU Chemical Engineering, Technical University of Denmark 4 June 2018

11 Macroalgae/seaweeds Value chains: Components: Fucoidans Proteins Antioxidants Alginate Laminarin Phytohormons Minerals Products: Food and Feed ingredients & flavours Health promoting compounds (prebiotics) Skin care & Cosmetics Wound healing New drug candidates?

12 Combined use of blue, green & grey feed stocks Company: Fermentation Experts Rape seed press pulp and macroalgae Co-fermented with lactic acid bacteria Combining two effects, prebiotics and probiotics => improvement of the gut flora

13 Timing Ancient wisdom in new focus: *Gut health is determined by gut microbiota and gut barrier *Gut health has significant impact on general health Picture Credit: lostrestesoros Credit: lostrestesoros

14 New: Highest value use of macroalgae (co-fermented seaweed & rape seed pulp) Pig feeding trials, large scale, in Poland ( ) New Feeding strategy => making industrial pig production without antibiotics and without zinck possible (?) Clinical trials, inflammatory bowel disease ( ) New food supplement for lowering the symptoms of IBDpatients (Crohn s disease and Colitis) Next: fermented seaweed as gut-health promoting food supplement for common use (5 g per day is sufficient) Prebiotic and probiotic effect

15 Feed trials and Clinical trials Company expertise: Fermentation experts AS: Feed additive products, made by co-fermentation of macroalgae and rape seed /Jens Legarth, CEO and Founder New: Fermbiotics, Food Supplement product concept, made by co-fermentation of macroalgae and rape seed /Søren Kjærulf, CEO New clinical trial concepts for testing effect of pre- and probiotic food additive in chronic inflammatory diseases. Clinical trials, Silkeborg Hospital, DK /Jan Villadsen M.D. Specialist in Clinical Immunology, Immunology and Transfusion medicine Rikke Damsgaard&Henning Glerup,MD

16 Clinical trials: Professor Jan Villadsen & co-workers Silkeborg Regional Hospital, Diagnostic Centre, University Research Clinic for Innovative Patient Pathways Goal: Investigation the effect of macroalgae as a preand pro-biotic food additive on chronic, inflammatory bowl diseases, IBD

17 Microalgae High potentials: valuable components; high productivity But also outstanding issues: Production (cost & contamination) Harvesting (cost & cell damage => fouling)

18 Microalgae -a new industrial approach Controlled production In Bioreactors (biologically contained) GMO? Patenting/IP-protection possible value chains: oil, sugars and proteins Improved growth, yield and composition New, cascading processing: Harvest as paste (not dried) Recover protein, sugars and nutrients HTL treatment of the rest => Bio-Oil

19 Fast track to market -for new blue bioeconomy products blue bioeconomy itself can create the market for many of such new products based on its need for improved types of fish feed and feed ingredients stimulating health, diminishing the need for antibiotics and by being more accessible for the fish; all in all leading to a less polluting aquaculture

20 EXTRA New Focus, Enzyme Discovery from aquatic microorganisms:

21 PPR-based HotPep (Busk & Lange et al ) Sequence analysis technology platform Fast track discovery of new enzymes of specific function Add synergy to the new CAZy subfamilies Enabling prediction of function directly from sequence, hereby bridging between genomics and ecology & biology Enabling annotation of large metagenomes also PPR mining only for specific enzyme families/functions State of the art for interaction secretome studies: host/pathogen/symbiont; rhizosphere- and gut-microbiota 21 DTU Chemical Engineering, Technical University of Denmark 4 June 2018

22 GH13, α-amylase, EC : Zoosporic fungi have rich diversity! 22 DTU Chemical Engineering, Technical University of Denmark

23 UN Sustainable Development Goals will only be met if: * green& blue technologies are developed *fossils substituted by renewables *bio-resources used more efficiently for higher value, health promoting products DTU Chemical Engineering, Technical University of Denmark

24 Take home messages: The opportunities: Much more to gain from blue biomass Coastal Biorefineries, running year round Sense of Urgency: Global Climate Change challenged agriculture => Need for improved use of bio-resources International Collaboration Build&Share public knowledge and best practices Build smarter, targeted bio-business faster Think Global Act Local Lene 24 DTU Chemical Engineering, Technical University of Denmark 4 June 2018