Ph.D. student ONWAR Course Molecular Neurobiology

Transcription

1 1 Ph.D. student ONWAR Course Molecular Neurobiology 26 June 30 June 2017 Organizers: Joost Verhaagen Netherlands Institute for Neurosciences Meibergdreef BA Amsterdam telephone : (020) fax : (020) j.verhaagen@nin.knaw.nl Guus Smit Dept. of Molecular and Cellular Neurobiology VU University Faculty of Earth and Life Sciences De Boelelaan HV Amsterdam telephone : (020) fax : (020) guus.smit@cncr.vu.nl Graduate School Neurosciences Amsterdam Rotterdam

2 2 List of speakers Netherlands Institute for Neurosciences Inge Huitinga Meibergdreef 47 Joost Verhaagen BA Amsterdam Christiaan Levelt Matthew Mason Christian Lohmann Nawal Zabouri VU University, Dept. of Neurosciences Ronald van Kesteren Faculty of Earth and Life Sciences Guus Smit De Boelelaan 1087 Ka Wan Li HV Amsterdam Sabine Spijker Sander Groffen Ruud Toonen Danielle Posthuma Vivi Heine Laurens Witter Kyoko Watanabe University of Amsterdam, UVA Marten Smidt Postbus GE Amsterdam Rudolf Magnus Institute for Neurosciences Vamshi Vangoor P.O. Box Jinte Middeldorp TA Utrecht Pavol Zelina uniqure Pavlina Konstantinova Meibergdreef BA Amsterdam Hubrecht Laboratory Niels Geijsen Uppsalalaan CT Utrecht Netherlands Cancer Institute Titia Sixma t.sixma@nki.nl Plesmanlaan CX Amsterdam Leiden University Medical Center Peter Bram t Hoen p.a.c._t_hoen@lumc.nl International Guest Speakers: Swammerdam Lecture John Mattick Garvan Institute of Medical Research Sydney Australia

3 3 Locations: Lectures at the VU University: buildings and rooms are indicated in the program. Explanation locations: WN = Wis- en Natuurkunde = science building VUA, De Boelelaan 1085, Amsterdam For route description, please see: All lectures in the Netherlands Institute for Neuroscience are in the Colloquium Room at the third floor of the Institute at Meibergdreef 47, Amsterdam-Zuidoost. For route description, please see: (see: looproutes en parkeermogelijkheden)

4 4 Program Ph.D. course molecular neurobiology Monday 26 June VU/NIN Welcome and introductory remarks on Guus Smit *WN C638 the aim of the program Basics of molecular neuroscience: the gene and gene regulation Overview of molecular neuroscience Guus Smit * WN C638 Fundamental aspects of the structure of DNA and RNA, the basic organization of a gene, the process of transcription and translation Coffee Manipulation of genes/dna: vectors and their applications. Guus Smit * WN C638 DNA is hosted in vectors. Various types of vectors are available to researchers and their applications will be discussed, the polymerase chain reaction The structure and evolution of the genome: SNPs and CNVs Sander Groffen * WN C Lunch Basics of molecular neuroscience: the genome, what did we learn during the last 10 years? Non-coding RNA s: versatile regulators of gene expression Vamshi Vangoor * WN C FUMA Functional interpretation of genome-wide association results Kyoko *WN C638 Watanabe Travel to NIN Swammerdam lecture at the NIN NIN John Mattick: RNA is the computational engine of human development and brain function Drinks and Snacks

5 5 Tuesday 27 June 2017 VU *WN = Science buildingvu De Boelelaan 1085 Analysis of protein protein interactions and proteomics Protein interactions: Yeast-two-hybrid system versus mass spectrometry Guus Smit * WN C Genome Wide Association studies (GWAS) to decipher Danielle * WN C668 Complex brain disorders Posthuma Proteomics: analysis of the synaptic proteome Ka Wan Li * WN C Lunch Demonstration: mass spectrometry Ka Wan Li ips cells and cellular screening technology ( Cellomics ) ips Center: A stem cell culture and phenotype screening facility at the VU/VUMC Vivi Heine * WN C Tea Demonstration: stem cell and cellomics facilities Ronald van Kesteren/Vivi Heine

6 6 Wednesday 28 June NIN High-throughput gene expression analysis Introduction to micro-array technology Matthew Mason High-throughput RNA-seq - measuring all there is?!. Peter-Bram t Hoen Coffee Practical: working with a microarray/rnaseq data set Matthew Mason Lunch Live cellular imaging of tagged proteins Live imaging of GFP-tagged proteins in neurons Ruud Toonen Imaging structural plasticity and synaptic signaling in intact neuronal circuits Christian Lohmann Tea Technique: 3D-synapse visualization in cleared brain tissue Nawal Zabouri

7 7 Thursday 29 June NIN Gene manipulation and gene therapy Transgenic animal technology Christiaan Levelt Cold Spring Harbor video of transgenic mouse techniques Coffee Christiaan Levelt Gene knock-out technology Sander Groffen Viral vector technology Joost Verhaagen Lunch Gene therapy and nerve repair Joost Verhaagen Gene Therapy for Huntington disease: a clinical and industrial perspective Pavlina Konstantinova Tea Genome editing with a pinch of salt Niels Geijsen

8 8 Friday 30 June NIN Application of molecular biology to study basic neurobiological problems Protein crystallography: application in neurobiology Titia Sixma Dissecting neuropsychiatric disorders: the molecular approach Sabine Spijker Coffee Dopaminergic neurons: transcriptional determinants Marten Smidt Axon guidance molecules: signaling and circuit formation Pavol Zelina Lunch Application of molecular biology to study neurological diseases The molecular biology of Alzheimer's disease Jinte Middeldorp The Netherlands Brain bank: a resource of human brain tissue Inge Huitinga Optogenetics Laurens Witter End of Course