labkem CULTURE MEDIA FOR INDUSTRIAL MICROBIOLOGY

Transcription

1 CULTURE MEDIA FOR INDUSTRIAL MICROBIOLOGY

2 2016 Labkem includes culture media for Industrial Microbiology, divided into: I - GENERAL USE II - SPECIFIC USE III - INGREDIENTS IV - MOLECULAR BIOLOGY In this brochure LABKEM wants to present its entire range of culture media so that the user can know in a simple way which media to use in each application and which supplements to use with each medium. Listed below we indicate what industrial sector each microbiological parameter is controlled. Clostridium perfringens Coliforms Enterobacteria Escherichia coli Lactobacilus Listeria monocytogenes Salmonella / Shigella Staphylococcus aureus Meat & Fish Industry Water & Beverages Dairy Products Bakery Processed Foods Beer Industry Wines Waste Water Cosmetic Industry Pharmaceutical Industry

3 I - GENERAL USE CULTURE MEDIA GENERAL USE media are used in applications for a wide variety of species. Here are the different GENERAL USE culture media LABKEM offers, and what applications are used: Capacity Application method AGDS-0EP-500 Saboraud Dextrose Agar BAC Ph. Eur. Cultivation Ph. Eur AGSC-00P-500 Sabouraud Dextrose w/ Chloramphenicol Agar BAC Cultivation - Isolation AGST-0EP-500 Trypticasein Soy Agar (TSA) BAC Ph. Eur. General Bacteriology Methods Ph. Eur STRB-0EP-500 Trypticasein Soy Broth (TSB) BAC Ph. Eur. Cultivation Ph. Eur AGPT-0EP-500 Buffered Peptone Water BAC Ph. Eur. Diluent for the Homogenization of Samples Ph. Eur AGPT-0DI-500 Buffered Peptone Water BAC ISO-6579, ISO-22964, ISO-6887, ISO-19250, DIN-10181, DIN Diluent for the Homogenization of Samples ISO 6579 ISO ISO 6887 ISO DIN DIN 10160

4 II - SPECIFIC USE CULTURE MEDIA SPECIFIC USE culture media are used in applications for a specific species, or 2 species in some cases. Here are the different SPECIFIC USE culture media LABKEM offers, indicating which applications, in which industrial sector and for what species are used: Supplement Ref. Capacity Application Industry method Clostridium perfringens Coliforms Enterobacteria Species Escherichia coli Lactobacilus Listeria monocytogenes Salmonella / Shigella Staphylococcus aureus ABVG-IEP-500 ABVL-00I-500 Violet Red Bile w/ Glucose (VRBG) Agar BAC Ph.Eur, ISO Violet Red Bile w/ Lactose (VRBL) Agar BAC ISO-4832 Cultivation - Enumeration Food - Pharmaceutical - Water Ph. Eur ISO Detection - Enumeration Food - Water ISO 4832 AGCH-00I-500 TTCS-00P-010 Chapman TTC Agar BAC ISO Enumeration Water ISO AGTB-00I-500 Chromogenic Tryptone Bile -Glucuronide (TB) Agar BAC ISO ,3 Detection - Enumeration AGMC-IEP-500 Macconkey Agar BAC Ph. Eur., ISO Isolation - Identification Food - Pharmaceutical - Water Food ISO ISO Ph. Eur ISO AGMR-00P-500 Man, Rogosa and Sharpe (MRS) Agar BAC Cultivation Food AGSM-00I-500 Standard Methods Agar (PCA) BAC ISO-4833 Total microbial plate count Food AGMH-00P-500 Mueller Hinton Agar BAC Antibiotic sensitivity test Pharmaceutical - Health sector AGSP-00P-500 Sulfite Polymyxin Sulfadiazine (SPS) Agar BAC Isolation Food AGL-0EP-500 ylose Llysine Desoxycholate (LD) Agar BAC Ph. Eur Isolation ABBP-0EP-500 EYTS-00P-100 Baird Parker Agar Base BAC Ph. Eur. Isolation Pharmaceutical Ph. Eur FRLB-0HI-500 FACS-00I-010 FRLB-00I-500 FACS-00I-010 Listeria Enrichment 1/2 Fraser Broth Base BAC ISO Listeria Enrichment Fraser Broth Base BAC ISO Enrichment Food ISO Enrichment Food ISO RSVB-00P-500 Rappaport Soy Broth acc. Vassiliadis BAC Selective enrichment Food - Water BRGR-00I-500 Brilliant Green Bile Broth 2% (BRILA) BAC ISO-4831, ISO-4832 Detection Food - Water ISO 4831 ISO 4832 ABPA-00I-500 PLSS-00I-010 Listeria Agar Base Palcam BAC ISO Selective - differential Food - Water ISO

5 Supplement Ref. Capacity Application Industry method Clostridium perfringens Coliforms Enterobacteria Species Escherichia coli Lactobacilus Listeria monocytogenes Salmonella / Shigella Staphylococcus aureus RSVB-00I-500 MKFB-00I-500 AGL-00I-500 IODI-R0A / POIO-00A Rappaport Soy Broth acc. Vassiliadis BAC ISO-6579, ISO Mueller Kauffmann Broth Base with Brilliant Green and Novobiocine (MKTTN) BAC ISO-6579 ylose Llysine Desoxycholate (LD) Agar BAC ISO-6579, ISO-10273, ISO Selective enrichment Food - Water ISO 6579 ISO Selective enrichment Food ISO 6579 Selective isolation Food ISO 6579 ISO ISO AGCS-00P-500 Salmonella Chromogenic Agar BAC Isolation Food - Pharmaceutical AGTS-00I-500 LDBR-00I-500 Triple Sugar Iron Agar (TSI) BAC ISO-6579, ISO Lysine Decarboxylase Broth BAC ISO-6579, ISO-10273, ISO Confirmation Identification - Confirmation Food ISO 6579 ISO ISO 6579 ISO ISO ABO-00I-500 OLSS-00I-010 Listeria Agar Base Oxford BAC ISO Selective - Detection Food - Water ISO ABLC-00I-500 LCSS-00I-010 / LLCS-00I-010 Listeria Chromogenic Agar Base BAC ISO Detection - Enumeration Food - Water ISO ABBL-00P-500 BLPS-00P-010 Blood Agar Base BAC Cultivation - Isolation - Detection Health sector AGCC-00I-500 Coliforms Chromogenic Agar (CCA) BAC ISO :2014 Selective - Detection Water ISO SUPPLEMENTS PLSS-00I vials Palcam Listeria Selective Supplement BAC ISO Isolation Food - Water ISO TTCS-00P vials TTC 1% Supplement BAC Indicator Water EYTS-00P ml Egg Yolk Emulsion + Tellurite Supplement BAC Detection - Enumeration Pharmaceutical FACS-00P-010 OLSS-00I-010 LCSS-00I vials 10 vials 10 vials Ferric Ammonium Citrate Supplement BAC ISO Oxford Listeria Selective Supplement BAC ISO Listeria Chromogenic Selective Supplement BAC ISO Detección Food ISO Selective isolation Food - Water ISO Isolation - Detection - Enumeration Food - Water ISO LLCS-00I vials Listeria Lipase C Supplement BAC ISO Isolation - Detection - Enumeration Food - Water ISO BLPS-00P-010 2x5 viales Polyenrichment Supplement BAC Selective Health sector

6 II - SPECIFIC USE CULTURE MEDIA Among the species involved in the SPECIFIC USE culture media, we want to emphasize the two most widespread in the industrial field cases: Salmonella spp PRE-ENRICHMENT AGPT-0DI-500 Buffered Peptone Water BAC ISO-6579, ISO-22964, ISO-6887, ISO-19250, DIN-10181, DIN Sample enrichment in a non-selective nutrient medium, enabling the damaged Salmonella cells to be repaired, and achieving a stable physiological condition. SELECTIVE ENRICHMENT RSBR-00I-500 Rappaport Soy Broth acc. Vassiliadis BAC ISO-6579, ISO Achieved by the use of a media culture combining 2 conditions: Increment of the Salmonella populations Inhibition of other microorganisms present in the sample SELECTIVE ISOLATION AGL-00I-500 ylose Llysine Desoxycholate (LD) Agar BAC ISO-6579, ISO-10273, ISO Use of selective media which: Restrict the growth of types other than Salmonella Allow the visual recognition of suspicious colonies CONFIRMATION AGTS-00I-500 Triple Sugar Iron Agar (TSI) BAC ISO-6579, ISO LDBR-00I-500 Lysine Decarboxylase Broth BAC ISO-6579, ISO-10273, ISO Generic identification of Salmonella cultures and elimination of false suspicious cultures. Some media are to be used with the supplements listed in the table in section II.

7 II - SPECIFIC USE CULTURE MEDIA Listeria monocytogenes PRE-ENRICHMENT FRLB-0HI-500 Listeria Enrichment 1/2 Fraser Broth Base BAC ISO Sample enrichment in a non-selective nutrient medium, enabling the damaged Listeria monocytogenes cells to be repaired, and achieving a stable physiological condition. SELECTIVE ENRICHMENT FRLB-00I-500 Listeria Enrichment Fraser Broth Base BAC ISO Achieved by the use of a media culture combining 2 conditions: Increment of the Listeria monocytogenes populations Inhibition of other microorganisms present in the sample SELECTIVE ISOLATION ABO-00I-500 Listeria Agar Base Oxford BAC ISO ABPA-00I-500 Listeria Agar Base Palcam BAC ISO Use of selective media which: Restrict the growth of types other than Listeria monocytogenes Allow the visual recognition of suspicious colonies CONFIRMATION ABLC-00I-500 Listeria Chromogenic Agar Base BAC ISO Generic identification of Listeria monocytogenes cultures and elimination of false suspicious cultures. Some media are to be used with the supplements listed in the table in section II.

8 III - INGREDIENTS The ingredients are used as intermediates or additives for the formulation of culture media for wide range of applications and fields. Here are the different ingredients LABKEM offers: Capacity AGAG-00P-500 Agar Agar BAC YEE-0MB-500 Yeast extract BAC TRYN-00P-500 Peptone from Casein (Tryptone) BAC BACP-00P-500 Bacteriological peptone BAC **Within the range of dyes and stains LABKEM, you can also find ingredients involved in culture media formulations, such as crystal violet (GENT-V0D-025) or Lugol (LUGO-00A-250).

9 IV - CULTURE MEDIA FOR MOLECULAR BIOLOGY The culture media for MOLECULAR BIOLOGY are used in various processes such as bacterial transformation, growth, maintenance and isolation, protein expression, etc. Here are the different culture media for MOLECULAR BIOLOGY offered in LABKEM, indicating which applications are used in each case: Capacity Application AGLB-00P-500 LB (LENNO) Agar GEN TEST E.Coli AGLU-00P-500 LURIA Agar (Miller's LB Agar) GEN TEST E.Coli LBBR-00P-500 LB (LENNO) Broth GEN TEST E.Coli LUBR-00P-500 LURIA Broth (Miller's LB Broth) GEN TEST E.Coli TEBR-00P-500 Terrific Broth GEN Cultivo E.Coli / Cultivation E.Coli / Culture E.Coli AGAR-D1L-500 Agarose D1 LOW EEO (electroendosmosis) GEN Nucleic acid analytical and preparative electrophoresis - Blotting - Protein electrophoresis (Radial Immunodiffusion) AGAR-E Agarose E GEN Separations of DNA and RNA fragments as well as PCR products AGAR-D1L g Agarose LM-SIEVE GEN Electrophoresis of DNA fragments 1000 bp - In-Gel enzymatic processing (digestion, ligation, PCR) - Preparative electrophoresis - Analysis and recovery of small DNA fragments for further applications AGAR-MS g Agarose MS-4 GEN Improved resolution of DNA 500 bp

10 Within LABBO also we offer labware used in microbiological processes. Here are the references in a table of lab material that could be used in applications listed in this brochure: GFFC GFFA MCNE MCNE Glass microfiber filter, GFFC grade (1,2 um), Ø47 mm, 100 pcs Glass microfiber filter, GFFA grade (1,6 um), Ø47 mm, 100 pcs Cellulose nitrate membrane Ø 47 mm, Sterile, 0,45 µm, 100 pcs Cellulose nitrate membrane Ø 47 mm, Sterile, 0,20 µm, 100 pcs SLIU SLIG SLIF Microscope slides cut edges, Basic Line, 26x76mm ( ), corners 90, 50 pcs. Microscope slides ground edges, Basic Line, 26x76mm ( ), corners 45, 50 pcs. Microscope slides ground edges, single frosted, Basic Line, 26x76mm ( ), corners 90, 50 pcs. MNFA Stainless steel filter manifold, 3 branches SLIU Microscope slides cut edges Standard Line, 26x76mm ( ), 50 pcs. MNFA Stainless steel filter manifold, 6 branches SLIG Microscope slides ground edges Standard Line, 26x76mm ( ), 50 pcs. FUC FUSS LOOP-E01-1K0 LOOP-E Glass filter holder for 47 mm disc filters Sainless steel filter holder for 47 mm disc filters Inoculation loops, calibrated, 1 µl neutral color, sterile, 10 pcs/peel pack, 10 x 100 pcs/ pack Inoculation loops, calibrated, 1 µl neutral color, sterile, 1 pc/peel pack, 500 pcs/pack SLIF SLIU-D SLIF-D Microscope slides, ground edges, single frost, Standard Line, 26x76mm ( ), 50 pcs Microscope slides, cut edges, double frosted, Standard Line, 26x76mm ( ), corners 90, 50 pcs Microscope slides, ground edges, double frosted, Standard Line, 26x76mm ( ),corners 45,50 pcs LOOP-E10-1K0 LOOP-E PDIP-09N-500 PDIP-E9N-500 Inoculation loops, calibrated, 10 µl blue color, sterile, 10 pcs/peel pack, 1000 pcs/pack Inoculation loops, calibrated, 10 µl blue color, sterile, 1 pcs/peel pack, 500 pcs/pack Petri dishes, Ø90x15 mm, Polystyrene, aseptic, without vents, 500 pcs Petri dishes, Ø90x15 mm, Polystyrene, sterile, without vents, 500 pcs SLIB-G SLIB-F SLIB-B Microscope slides, beveled edges, Premium Line, 26x76mm ( ), 50 pcs Microscope slides, beveled edges, single frost, Premium Line, 26x76mm ( ), 50 pcs Microscope slides frosted end both sides, beveled edges, Premium Line, 26x76mm( ), 50 uds PDIP Petri dishes, Ø90x15 mm, Polystyrene, aseptic, with 3 vents, 500 pcs PDIP-E Petri dishes, Ø90x15 mm, Polystyrene, sterile, with 3 vents, 500 pcs PDIP Petri dishes, Ø90x14 mm, Polystyrene, aseptic, with 3 vents, 575 pcs PDIP-E Petri dishes, Ø90x14 mm, Polystyrene, sterile, with 3 vents, 575 pcs MSHN Staining jar, acc. to Hausser-Gedigk, for 30 slides MSHN Staining jar, acc. to Hausser-Gedigk, for 60 slides MSSN Staining jar, acc. to Coplin, for 5 slides MSSN Staining jar, acc. to Schieferdecker, for 10 slides MSSN Staining jar, acc. to Hellendahl, for 8 slides **To see all the options offered for microbiological applications by LABBO, consult the catalog Labbox 2016.