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1 47 1 () Vol. 47 No Journal of Xiamen University (Nat ural Science) Jan , 2, 2, 1 3 (1., ;2., ) :, cells/ ml., :., m,,,, 0. 66,45. 3 % ;, m,,,0. 40,,54. 7 %.,. : ;; ; :Q :A : (2008) ( S epiella m ai nd roni),,, ( Cep halopo2 da),(decapoda),( Sepiidae),.,, ;,,,,,;,.,,,. [1-5 ],,., [6 ]..,,, [7 ].,,, , : :(2006DFA32960), (2006C14017) 3 edu. cn, 2122, ( 2. 8) cm,275 ( 35) g, ml 5, mol/ L ( PBS, p H7. 4) 2 3., ml (1) 1 ml 0. 1 ml 10 %,0. 1 ml,,,. (2) 1 ml 5,, 15 min,, 10 min,. (3) 1 ml 5,, , (Ni2 kon) DXM1200F. 1. 6, :

2 1 : 95 1 A :;B :;C :;S : ; P : ;Bar :5m Fig. 1 Observation of living haemocytes of S epiella maind roni = ( ) / ( ) ,,,. 37 min,, min,,, 8 m,, (12A) ;,,,, (12B,C). 2. 2,,,. 2 3,,, ; 1,10 %, (2),. 2 Bar :5m Fig. 2 Fixed haemocytes by formaldehyde (32A,B,C)., ;, , cells/ ml ( cells/ ml). 3 3 A : ;B :;C : ;Bar :7m Fig. 3 Staining of Sepiella maind roni haemocytes

3 96 () A : ;B :;C : ;Bar :5m Fig. 4 Staining of Sepiella maind roni hyalinocytes 5 A : ;B :;C : ;Bar :5m Fig. 5 Staining of Sepiella maind roni granulocyte,, 2,45. 3 %, %(1). (1) :,, ( 0. 12) ( 0. 08) m,,,,, ( 0. 10).,.,,, ;, (42A,B,C). (2) :,, ( 0. 20) ( 0. 42) m, 2,,,0. 40 ( 0. 06).,,.,,,;,(52A,B, C). 3,,,,,. 3, 10 %,,,,.. Foley [8 ] Auff ret [9 ] ( Crassost rea vi r2 1 Tab. 1 Dimensions of S. maind roni hemocytes measured in a smear /m /m H ( n = 50) ( 0. 12) ( 0. 08) ( 0. 35) ( 0. 68) ( 0. 10) G( n = 50) ( 0. 20) ( 0. 42) ( 0. 62) ( 1. 24) ( 0. 06) : H : ; G: ; n :.

4 1 : 97 gi nica) ( C. gi g as) 2. Renwrantz [10 ]. Cheng [11 ] ( Mercenari a mercenari a),. Pipe [12 ] ( M y til us edulis). Maria [13 ],( M. gallop rovi nci alis) 3 :,. Moore [14 ]., :. 3, ,,.,3,,,.,. [15 ],. 2.,.,,,,.,.,Mix [16 ]. [17 ], [18 ].,,. : [1 ],. [J ].,1990,14 (2) : [2 ],,,. [ M ]. :,1993 : [3 ],,,. [J ].,1997 (5) : [4 ],,. [J ]. :, 2002, 32 (5) : [5 ],,. [J ].,2005,8 (4) : [6 ],. [J ].,1997,21 (4) : [7 ] Pipe R K. Differential binding of lectins to haemocytes of the mussel M ytil us edulis [J ]. Cell and Tissue Research, 1990,261 (2) : [8 ] Foley D A,Cheng T C. Interaction of molluscs and foreign substances :the morphology and behavior of hemolymph2 cells of the American oyster, Crassost rea vi rginica, in vitro[j ]. J Invertebr Pathol,1972,19 (2) : [9 ] Auffret M. Comparative of the hemocytes of two oyster species : the european flat oyster, Ost reacdulis, linnaeus, 1750 and the Pacific oyster, Crasost rea gi gas ( thunberg, 1793) [J ]. J of Shellfish Research,1989,8 (2) : [10 ] Renwrantzl, Yoshino T,Cheng T C,et al. Size determi2 nation of hemocytes f rom the American oyster, Cras2 sost rea vi rginica, and the description of a phagocytosis mechanism[j ]. Zool J b Physiol,1979,83 (1) :1-12. [11 ] Cheng T C, Foley D A. Hemolymph cells of the bivalve mollusc Mercenaria mercanaria :an electron microscopi2 cal study[j ]. J Invertebr Pathol,1975,26 (4) : [12 ] Pipe R K,Livingstone D R. Antioxidant enzymes associ2 ated with the blood cells and haemolymph of the mussel Myritus edulis[j ]. Fish Shellfish Immunol,1993,3 (3) : [13 ] Maria J C. Hemolymph cell types of the mussel M ytil us gallop rovincialis [ J ]. Diseasa of Aquatic Organism, 1997,29 (2) : [14 ] Morre M N,Lowe D W. The cytology and cytochemostry of the hemocytes of M ytil us edulis and their response to experimentally injected carbon particles [ J ]. J Invertebr Pathol,1977,29 (1) : [15 ],. [J ].,2001,11 (4) : [16 ] Mix M C A. Generalmodel for leucocyte cell renewal in bivalve mollusks[j ]. Mar Fish Rew,1976,38 (1) : [17 ] Xue Q, Renault T. Monoclonal antibodies to European

5 98 () 2008 flat oyster Ost rea edulis hemocytes :characterization and tissue distribution of granulocytes in adult and develo2 ping animals[j ]. Developmental and Comparative Immu2 nology,2001,25 (3) : [18 ] Sahaphong S,Linthong V,Wanichanon C,et al. Morpho2 f unctional study of the hemocytes of H aliotis asinins [J ]. Journal of Shellfish Research, 2001, 20 (2) : Classif ication and Morphological Observation on Haemocytes in Se piella maindroni ZHAN G Jian2she 1,WU Chang2wen 2,C HAN G Kang2mei 2,SU Yong2quan 1 3 (1. College of Oceanography and Environmental Science,Xiamen University,Xiamen ,China ; 2. Marine Science College,Zhejiang Ocean. University,Zhoushan ,China) Abstract : Study on the morphology of the haemocytes of the cuttlefish Se piella maind roni was conducted by the method of histo2 logical staining in this paper. The average density of haemocytes was cells/ ml. The haemocytes of the cuttlefish S. main2 d roni were classified into two types, hyalinocytes and granulocytes,according to the characteristics of the haemocytes. Hyalinocytes with the size of m,were roundness,the nucleolus were also roundness surrounded by relatively small cytoplasm,there were little granule in cytoplast,the nucleus/ cytoplasm (N/ C) ratio was higher ( ),and the proportion of haemocytes was % in the total haemocytes. Granulocytes with the size of m were roundness,there were much granules in the en2 doplasm and the N / C ratio was lower ( ),some of the granulocytes had double nuclei,and the proportion of granulocytes was % in the total haemocytes. This research might be a foundation for further study on haemocytes immune of S. maind roni. Key words : Se piella maind roni ;haemocytes ;morphology ;classification