ANTITHROMBIN FACTOR Xa

Transcription

1 2014

2 ATITRMBI FACTR Xa The Coamatic Antithrombin kits are simple, accurate and convenient. All methods are based on inhibition. Allows accurate determination of antithrombin in patients receiving heparin therapy. Superior reagent stability. Validated and documented protocols for automated instruments. Suitable for both large and small laboratories. CAMATIC ATITRMBI CAMATIC LR ATITRMBI LIQUID REAGET FRMAT ATITRMBI 10 IU A chromogenic kit for the determination of antithrombin activity in human plasma. The use of factor Xa in preference to thrombin eliminates interference from heparin cofactor II and thrombin inhibitors. AT + eparin [AT eparin] [AT eparin] + (excess) [AT eparin ] + (residual) (residual) Peptide + pa Substrate 1 vial 6 months 2-8 C Buffer with heparin 1 vial 3 months 2-8 C 1 vial 3 months 2-8 C umber of determinations Test tube method 50 Microplate method 200 Automated methods up to 130 Article number: K A chromogenic kit for the determination of antithrombin activity in human plasma. The use of factor Xa in preference to thrombin eliminates interference from heparin cofactor II and thrombin inhibitors. Reagents in liquid formulation. AT + eparin [AT eparin] [AT eparin] + (excess) [AT eparin ] + (residual) (residual) S-2772 Peptide + pa Substrate S vials 6 months 2-8 C with heparin 6 vials 1 month 2-8 C umber of determinations Automated methods up to 450 Article number: K Composition and purity Lyophilised powder prepared from bovine plasma and purified by barium citrate adsorption and liquid chromatography. Activation is performed by matrix bound activator from Russell s Viper Venom. Contains buffer salts, albumin and polyethyleneglycol. The activity (71 nkat) is determined with the substrate S Included in Coatest eparin Package 10x71 nkat Article number: B Composition and purity Lyophilised powder prepared from human plasma after affinity chromatography on heparin-sepharose gel. Contains human albumin as a stabiliser. Included in Coatest eparin Package 1x10 IU ote: This preparation is not a standard. Article number: B ATITRMBI 25 IU Composition and purity Lyophilised powder prepared from human plasma after affinity chromatography on heparin-sepharose gel. Pure preparation, does not contain stabiliser. Package 1x25 IU ote: This preparation is not a standard. Article number: B

3 APC RESISTACE PRTEI C igh discrimination between genotypes with 100% sensitivity for FV:Q 506. Reduces need for PCR determination. Applicable to anticoagulant treated patients. Detects the APC resistance phenotype regardless of its cause. Detects both inherited and acquired APC resistance. CATEST APC TM RESISTACE V CATEST APC TM RESISTACE V-S CATEST APC TM RESISTACE CAMATIC PRTEI C An APTT-based kit for screening of factor V-related APC resistance. The high sensitivity and specificity of the test for the factor V:Q 506 mutation is obtained by prediluting the sample plasma with an excess of V-DEF Plasma. The test design makes it possible to discriminate between heterozygous and homozygous factor V genotypes. It also allows for analysis of plasma from patients on heparin or oral anticoagulant therapy. ne volume of plasma is prediluted with four volumes of V-DEF Plasma. The dilution is then incubated with the APTT reagent for a standard period of time. Coagulation is triggered by the addition of CaCl 2 in the absence and presence of exogenous APC and the time for clot formation is recorded. V-DEF Plasma 4x4 ml (V) 2x4 ml (VS) 8 hours C 24 hours 2-8 C CaCl 2 1x8 ml (V) 2x2 ml (VS) 1 week C 1 month 2-8 C APTT reagent 1x16 ml (V) 2x4 ml (VS) 1 week C 1 month 2-8 C APC/CaCl 2 4x2 ml (V) 2x2 ml (VS) 8 hours 5 days C 2-8 C Control Plasma Level 1 1x1 ml 6 hours 2-25 C Control Plasma Level 2 1x1 ml 6 hours 2-25 C umber of determinations (V) (VS) Article number: K (V) K (VS) An APTT-based assay for the detection of the APC resistance phenotype, i.e. the poor anticoagulant response to activated protein C (APC). The test result (APC ratio) gives an estimation of the anticoagulant function in vivo and provides information on the thrombotic risk associated with inherited and acquired APC resistance. Plasma is incubated with the APTT reagent for a standard period of time. Coagulation is initiated by the addition of CaCl 2 in the absence and presence of APC and the time for clot formation is recorded. CaCl 2 1x8 ml 1 week 1 month C 2-8 C APTT reagent 1x16 ml 1 week C 1 month 2-8 C APC/CaCl 2 4x2 ml 8 hours 5 days C 2-8 C umber of determinations Automated methods Article number: K CTRL PLASMA LEVEL 1 CATEST APC TM RESISTACE Composition Lyophilised citrated, stabilised human plasma prepared from pools of plasma collected from healthy donors. Application Quality control of Coatest APC TM Resistance and Coatest APC TM Resistance V. Package Article number: C x1 ml A chromogenic kit for the determination of protein C activity in human plasma. o influence of heparin levels up to 3 IU/ml. Protein C activator Protein C APC S-2366 Peptide+pA CTRL PLASMA LEVEL 2 CATEST APC TM RESISTACE Composition Lyophilised citrated, stabilised human plasma prepared from pools of plasma collected from donors carrying the Factor V:Q 506 mutation. Application Quality control of Coatest APC TM Resistance and Coatest APC TM Resistance V. Package Article number: C x1 ml Activated Protein C (APC) Substrate S vials 3 months 2-8 C Protein C activator 2 vials 3 months 2-8 C umber of determinations Test tube method 72 Microplate method 288 Automated methods up to 180 Article number: K

4 CAMATIC EPARI EPARI A chromogenic kit for the determination of heparin and low molecular weight (LMW) heparin in human plasma. ne-stage assay optimized for a wide range of instruments. [AT eparin] [ AT eparin] + S-2732 Peptide + pa Substrate S vials 6 months 2-8 C Factor Xa 2 vials 3 months 2-8 C umber of determinations Test tube method 50 Microplate method 200 Automated methods 120 Article number: K SAMPLE DILUTI REQUIRED CATEST EPARI A chromogenic kit for the determination of heparin and low molecular weight heparin in human plasma. eparin + AT (excess) [eparin AT] [eparin AT] + (excess) [eparin AT ] + (residual) (residual) S-2222 Peptide + pa Substrate S vial 6 months 2-8 C Factor Xa 1 vial 1 month 2-8 C 6 months -20 C Antithrombin 1 vial 1 month 2-8 C 6 months -20 C Buffer 1 vial 2 months 2-8 C ormal plasma 4 vials 2 weeks 2-8 C 1 month -20 C umber of determinations Test tube method 100 Microplate method 400 Automated methods up to 285 Article number: K CALIBRATI PLASMA-LMW EPARI Composition Lyophilised human plasma, prepared by addition of heparin. Calibrated against the 1st International W standard (LMW heparin). Application For construction of calibration curves for use in chromogenic heparin assays. Package calibrator 1 4x1 ml calibrator 2 4x1 ml calibrator 3 4x1 ml Article number: C CTRL PLASMA-LMW EPARI Composition Lyophilised human plasma prepared by addition of LMW heparin. Calibrated against the 1st International W standard (LMW heparin). Application Quality control of chromogenic heparin assays. Package Low 4x1 ml igh 4x1 ml Article number: C

5 FACTR VIII The Factor VIII kits have two measuring ranges. Validated and documented protocols for automated instruments. All the Factor VIII kits can be used for the potency estimation of Factor VIII according to the European Pharmacopoeia requirements. CAMATIC FACTR VIII CATEST SP FACTR VIII EW KIT WIT IGER SESITIVITY CATEST SP4 FACTR VIII EW KIT WIT IGER SESITIVITY A chromogenic kit for the determination of factor VIII activity in human plasma, blood fractions and purified preparations. Fulfills the requirements of the European Pharmacopoeia for factor VIII concentrate testing. Factor X FIXa, Ca 2+, phospholipid FVIII Peptide + pa +I vial 1 month 2-8 C Factor reagent 2 vials 1 day 2-8 C 1 month -70 C Buffer 1 vial 1 month 2-8 C umber of determinations Test tube method 30 Microplate method 120 Automated methods up to 100 Article number: K Classic chromogenic kit for the determination of factor VIII activity in human plasma, blood fractions and purified preparations. Replaces Coatest Factor VIII (K821033). Factor X FIXa, Ca 2+, phospholipid FVIII Peptide + pa +I vial 3 months 2-8 C FIXa+FX 1 vial 12 hours 2-8 C CaCl 2 1 vial 3 months 2-8 C Buffer 1 vial 3 months 2-8 C Phospholipid 1 vial 3 months 2-8 C umber of determinations Test tube method 60 Microplate method 240 Automated methods up to 200 Article number: K Chromogenic kit for the determination of FVIII activity in human plasma, blood fractions and purified preparations. Suitable for low volume testing. Replaces Coatest FVIII:C/4 (K821918). Factor X FIXa, Ca 2+, phospholipid FVIII Peptide + pa +I vial 3 months 2-8 C FIXa+FX 4 vials 12 hours 2-8 C CaCl 2 1 vial 3 months 2-8 C Buffer 1 vial 3 months 2-8 C Phospholipid 1 vial 3 months 2-8 C umber of determinations Test tube method 4x15 Microplate method 240 Automated methods up to 200 Article number: K Co-lyophilisation of reagents for convenient handling. Reliable tool for haemophilia classification. o interference from heparin levels up to 1.5 IU/ml. 5

6 CRMGEIC SUBSTRATES Analyte Specific Reagents S-2222 TM S-2238 TM S-2251 TM S-2288 TM Chromogenic substrate for factor Xa. Chromogenic substrate for thrombin. Chromogenic substrate for plasmin and streptokinase-activated plasminogen. Chromogenic substrate for t-pa and a broad spectrum of other serine proteases. Article number: S Article number: S Article number: S Article number: S S-2222 Formula: Bz-IIe-Glu(γ-R)-Gly-Arg-pA Cl R= (50%) and R=C 3 (50%) Molecular weight: S-2238 Formula: -D-Phe-Pip-Arg-pA 2Cl Molecular weight: S-2251 Formula: -D-Val-Leu-Lys-pA 2Cl Molecular weight: S-2288 Formula: -D-Ile-Pro-Arg-pA 2Cl Molecular weight: R C 3 C 3 Cl 2 2 Cl 2 Cl C C 3 Cl 2 3 C C 3 2 Cl 2 Cl 2 3 C 3 C Cl 2 2 Well characterized substrates for many serine proteases in the coagulation and fibrinolytic system. 6

7 CRMGEIC SUBSTRATES Analyte Specific Reagents S-2302 TM S-2366 TM S-2403 TM TM Chromogenic substrate for plasma kallikrein and factor XIIa. Chromogenic substrate for activated protein C and factor XIa. Chromogenic substrate for plasmin and streptokinase-activated plasminogen. Chromogenic substrate for factor Xa. Article number: S Article number: S Article number: S Article number: S S-2302 Formula: -D-Pro-Phe-Arg-pA 2Cl Molecular weight: S-2366 Formula: pyroglu-pro-arg-pa Cl Molecular weight: S-2403 Formula: pyroglu-phe-lys-pa Cl Molecular weight: Formula: Z-D-Arg-Gly-Arg-pA 2Cl Molecular weight: Cl 2 Cl 2 2 Cl Cl 2 Cl 2 Cl ighly sensitive and selective. Validated methods for enzyme measurement available. 7

8 E CMPAY FR ALL YUR EMSTASIS EEDS ML REV03