MGC premier cdnas. The best value for ready-to-express cdna clones. Highest quality, comprehensive coverage, cost effective = Best Value

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1 The best value for ready-to-express cdna clones MGC premier MGC premier cdnas Highest quality, comprehensive coverage, cost effective = Best Value

2 MGC premier cdna and ORFs Choose from Full-Length, Sequence Verified cdna, Entry ORFs and Tagged ORFs Largest Collection of Pre-made, Full-Length cdna Clones MGC premier cdna transomic technologies provides you with the highest sequence quality and confidence when purchasing pre-made, full-length cdna clones. All cdna inserts have been rigorously sequenced and are backed by a 100% guarantee to be an exact match to the published sequence. (MGC Project Team, 2009). Full-length sequence guaranteed Ideal for native protein expression Ships in 24 hours Human, mouse, rat, bovine, Xenopus and Zebrafish genomes represented $ best 99 value Quick Reference Selection Guide Clonal Isolation and End-Sequence Option Want to confirm the identity of your cdna before shipping? We can streak isolate a colony and end sequence it before delivery. Select the end sequenced format when choosing your cdna from FETCH my gene. This risk-free option ensures you start with a verified clonal culture. H=Human, M=Mouse, R=Rat, B=Bovine, X=Xenopus, Z=Zebrafish Genes Cones Human Mouse Rat Bovine X. laevis X. tropicalis Fully Sequenced cdna Clones Across Model Organisms Select individual cdna clones, sets or complete genomes. support@transomic.com for custom sets.

3 Expression-Ready cdna Clones for Faster Gene Analysis MGC premier Expression-Ready cdna Best option for native protein expression Fully sequenced - better confidence in your results Strong promoter - robust expression Choice of selectable marker - transient or stable expression ptcn/ptcp vector schematic Entry ORF Clones - Easily Transfer to Your Vector of Choice MGC premier ORFs Open reading frame (ORF) clones contain only the protein coding sequence allowing for the addition of fusion tags. The MGC premier entry ORF collection represents the human genome with various mouse and Zebrafish genes also represented. Gateway adapted - easily transfer to a destination vector of choice Stop codon option - allows the addition of N or C-terminal fusion tags Fully sequenced - better confidence in your results Available as individual clones, sets or entire genome Guaranteed Expression & Guaranteed Sequence Express with Choice of Tag MGC premier Tagged ORFs The importance of full insert sequencing... Did you know? Choice of expression-ready cdna vectors One of the first documented SNPs is the underlying mutation causing sickle cell anemia. A single nucleotide alters cellular phenotype as well as a patient s life span (Ingram 1957). If left unchecked a cdna clone bearing this mutation would dramatically alter the outcome of an experiment. Every MGC premier cdna has been fully sequenced and all sequences are available in the clone details page of your gene search results when using FETCH my gene. Express your protein of interest with either Myc or FLAG fusion tags. Expression-ready - validated tagged vectors Choice of fusion tags - additional functionality Guaranteed expression - better confidence in your results Human, Mouse and Rat genomes Vector schematic shows a C-terminal Myc tag ORF. MGC premier Tagged ORF clones are available in N or C-terminal fusions. (Peptide sequence of the DDK tag is DYKDDDDK. FLAG is a registered trademark of Sigma- Aldrich)

4 Arrayed cdna and ORF Sets Offer Great Value Gene Family and Pathways Focused Sets cdnas and ORFs are available as focused sets representing popular gene families and pathways or as custom arrayed sets for gene analysis studies. Sets are amenable to focused gene analysis studies or screens and provide the largest saving per clone. Formats Available: cdna clones - Expression-ready format eliminates need for cloning. ORF clones Gateway adapted for efficient transfer to your vector of choice. The table below lists available cdna and ORF gene family and pathway sets. Use to Find the cdna or ORF Clone for Your Gene of Interest. FETCH my gene search tool is designed to help you easily find clones for your gene of interest and confirm your results using the gene information provided. Use gene accession, gene symbol or gene ID to begin your gene search. 1. Search Input your search term into FETCH my gene. 2. Choose product type Quickly filter product type using tabs. 3. Confirm gene and product Contact for gene and clone information. Validate your gene of interest with additional gene information provided. Clone specific information provided through link to clone details page. 4. Add to cart Click add to cart to purchase or continue shopping. Don t see your gene family or pathway of interest? Contact info@transomic.com for custom set options. Visit for more details. MGC premier cdna and ORF clones are available as individual clones, expression-ready clones, gene family and pathway sets and genome libraries. Get more information at /MGCpremier References: MGC Project Team, The completion of the Mammalian Gene Collection (MGC). Genome Res. 19: and Supplemental Research Data. Ingram VM (1957) Gene mutations in human haemoglobin: the chemical difference between normal and sickle cell haemoglobin. Nature. 1957, 180(4581):326-8.

5 Headquarters Hudson Alpha Institute for Biotechnology 601 Genome Way, Suite 1222 Huntsville, AL Phone: Fax: International Distributors Europe Asia Pacific France Ozyme Phone : +33 (0) Fax : +33 (0) info@ozyme.fr Germany, Austria and Switzerland BioCat Phone: +49 (0) Fax: +49 (0) info@biocat.com Italy EuroClone S.P.A. Phone: Fax: info.bio@euroclone.it Scandanavia and Benelux Westburg BV Phone: (+31) Belgium: Fax: (+31) info@westburg.eu United Kingdom and Republic of Ireland Cambridge Bioscience Ltd. Phone +44 (0) Fax: +44 (0) sales@bioscience.co.uk European countries that are not shown should contact: BioCat Phone: +49 (0) Fax: +49 (0) info@biocat.com Australia and New Zeland Integrated Sciences Phone: Toll-free: Fax: tech@integratedsci.com.au China (Hong Kong and Macau) Gene Company Limited Phone: (852) Fax: (852) info@genehk.com India SAF Labs Pvt. Ltd. Phone: (0) Fax: (0) info@saflabs.com Japan Funakoshi Phone: Fax: reagent@funakoshi.co.jp Taiwan GenDiscovery Biotechnology, Inc. Phone: Fax: gene@gendiscovery.com.tw