ExProfile TM Human Osteogenesis Related Gene qpcr Array

Transcription

1 ExProfile TM Human Osteogenesis Related Gene qpcr Array For focused group profiling of human osteogenesis genes expression Cat. No. QG046-A (1 x 96-well plate, Format A) Cat. No. QG046-B (1 x 96-well plate, Format B) Cat. No. QG046-C (1 x 96-well plate, Format C) Cat. No. QG046-D (1 x 96-well plate, Format D) Cat. No. QG046-E (1 x 96-well plate, Format E) Plates available individually or as a set of 6. Each set contains 84 unique gene primer pairs deposited in one 96-well plate. Introduction The ExProfile human osteogenesis related gene qpcr array profiles the expression of 84 human genes related to osteogenic differentiation. These genes are carefully chosen for their close correlation based on a thorough literature search of peer-reviewed publications, mainly including genes involved in development of the skeletal system as well as bone mineral metabolism. This array allows researchers to study the related genes to gain understanding of their roles in the functioning and characterization of osteogenic differentiation. QG046 plate 01: 84 unique gene PCR primer pairs Shipping and storage condition Shipped at room temperate Stable for at least 6 months when stored at -20 C Array format GeneCopoeia provides five qpcr array formats (A, B, C, D, and E) suitable for use with the following realtime cyclers. Important note: Upon receiving, please check to make sure that the correct array format was ordered to ensure the compatibility with your qpcr instrument. Plate format Instrument provider qpcr instrument model A (96-well) Applied Biosystems 5700, 7000, 7300, 7500, 7700, 7900HT (Standard 96-well block), ViiA TM 7 (Standard 96-well block) B (96-well) Applied Biosystems C (96-well) Bio-Rad Laboratories icycler iq,myiq,iq 5 D (96-well) Bio-Rad Laboratories 7500 (Fast block), 7900HT (Fast block), StepOnePlus TM, ViiA TM 7 (Fast block) CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 E (96-well) Roche Applied Science LightCycler 480 (96-well block) 1

2 Quality control 1. Each pair of primers in the ExProfile gene qpcr array has been experimentally validated to yield a single dissociation curve peak and to generate a single amplicon of the correct size for the targeted gene. 2. The positive PCR controls (PCR) have been verified to amplify a single amplicon of the correct size with Ct values around 20±2. 3. The Spike-in reverse transcription controls (RT) have been verified to amplify a single amplicon of the correct size with Ct values around 20±3. 4. R 2 > 0.99 was observed for high inter/ intra-array reproducibility. Materials required but not provided All-in-One TM First-Strand cdna Synthesis Kit All-in-One TM qpcr Mix Total RNA extraction kit (RNAzol RT RNA extraction reagent is recommended) DNase/RNase free tips, PCR reaction tubes, 1.5 ml microcentrifuge tubes 5 ml and 10 ml graduated pipettes, beakers, flasks, and cylinders 10 μl to1,000μl adjustable single channel micropipettes with disposable tips 5 μl to20μl adjustable multichannel micropipette, disposable tips, and reservoir qpcr instrument, compatible with gene qpcr arrays ordered Array layout Figure1. Illustration of QG046 plate 01 Gene primer pairs: 84 wells (A row to G row) are designated for a real-time PCR assay for genes (see the primer list). HK1-6: Six pre-deposited housekeeping gene (HK1-6) primer pairs, which can be used as endogenous positive controls as well as for array normalization. GDC: Genomic DNA controls, which can be used to specifically detect genomic DNA contamination with a high level of sensitivity. RT: Spike-in reverse transcription controls, which can be used to monitor the efficiency of the RT reactions. These pre-deposited primer pairs specifically amplify the cdna template reversed transcribed from the spike-in control RNA in the sample. PCR: Positive PCR controls, which are used to verify the PCR efficiency by amplifying the predeposited DNA template with its specific pre-deposited primer pairs. 2

3 Gene primer list Plate Position Catalog No. of Primer Accession No. of Gene Symbol QG A01 HQP NM_ RUNX2 QG A02 HQP NM_ FN1 QG A03 HQP NM_ FGFR1 QG A04 HQP NM_ HPRT1 QG A05 HQP NM_ VEGFA QG A06 HQP NM_ VDR QG A07 HQP NM_ VCAM1 QG A08 HQP NM_ TWIST1 QG A09 HQP NM_ TUFT1 QG A10 HQP NM_ TNF QG A11 HQP NM_ TGFBR1 QG A12 HQP NM_ TGFB3 QG B01 HQP NM_ TGFB2 QG B02 HQP NM_ TGFB1 QG B03 HQP NM_ TFIP11 QG B04 HQP NM_ SOX9 QG B05 HQP NM_ SMAD4 QG B06 HQP NM_ SMAD3 QG B07 HQP NM_ SMAD2 QG B08 HQP NM_ SMAD1 QG B09 HQP NM_ SERPINH1 QG B10 HQP NM_ SCARB1 QG B11 HQP NM_ RUNX2 QG B12 HQP NM_ PHEX QG C01 HQP NM_ PDGFA QG C02 HQP NM_ NFKB1 QG C03 HQP NM_ MMP9 QG C04 HQP NM_ MMP8 QG C05 HQP NM_ MMP10 QG C06 HQP NM_ MINPP1 QG C07 HQP NM_ ITGB1 QG C08 HQP NM_ ITGAM QG C09 HQP NM_ ITGA2 QG C10 HQP NM_ ITGA1 QG C11 HQP NM_ IGF2 QG C12 HQP NM_ IGF1R QG D01 HQP NM_ IGF1 QG D02 HQP NM_ ICAM1 QG D03 HQP NM_ FN1 QG D04 HQP NM_ FLT1 3

4 4 Product Data Sheet QG D05 HQP NM_ FGFR2 QG D06 HQP NM_ FGF2 QG D07 HQP NM_ FGF1 QG D08 HQP NM_ EGFR QG D09 HQP NM_ EGF QG D10 HQP NM_ DSPP QG D11 HQP NM_ DMP1 QG D12 HQP NM_ CTSK QG E01 HQP NM_ CSF3 QG E02 HQP NM_ CSF2 QG E03 HQP NM_ COMP QG E04 HQP NM_ COL5A1 QG E05 HQP NM_ COL3A1 QG E06 HQP NM_ COL2A1 QG E07 HQP NM_ COL1A1 QG E08 HQP NM_ COL15A1 QG E09 HQP NM_ COL14A1 QG E10 HQP NM_ COL12A1 QG E11 HQP NM_ COL11A1 QG E12 HQP NM_ CDH11 QG F01 HQP NM_ CD36 QG F02 HQP NM_ CALCR QG F03 HQP NM_ BMP5 QG F04 HQP NM_ BMP4 QG F05 HQP NM_ BMP3 QG F06 HQP NM_ BMP2 QG F07 HQP NM_ BMP1 QG F08 HQP NM_ BGN QG F09 HQP NM_ BGLAP QG F10 HQP NM_ ANXA5 QG F11 HQP NM_ AMBN QG F12 HQP NM_ ALPL QG G01 HQP NM_ MSX1 QG G02 HQP NM_ COL12A1 QG G03 HQP NM_ TGFBR2 QG G04 HQP NM_ VEGFB QG G05 HQP NM_ EGFR QG G06 HQP NM_ COL10A1 QG G07 HQP NM_ COL1A2 QG G08 HQP NM_ COL4A3 QG G09 HQP NM_ ENAM QG G10 HQP NM_ FGF3 QG G11 HQP NM_ FGFR1 QG G12 HQP NM_ GDF10 QG H01 HGDC QG H02 HGDC

5 QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR 5

6 Limited Use License Product Data Sheet Following terms and conditions apply to use of ExProfile TM Human Osteogenesis Related Gene qpcr Array (the Product). If the terms and conditions are not acceptable, the Product in its entirety must be returned to GeneCopoeia within 5 calendar days. A limited End-User license is granted to the purchaser of the Product. The Product shall be used by the purchaser for internal research purposes only. The Product is expressly not designed, intended, or warranted for use in humans or for therapeutic or diagnostic use. The Product must not be resold, repackaged or modified for resale, or used to manufacture commercial products or deliver information obtained in service without prior written consent from GeneCopoeia. This Product should be used in accordance with the NIH guidelines developed for recombinant DNA and genetic research. Use of any part of the Product constitutes acceptance of the above terms. Limited Warranty GeneCopoeia warrants that the Product meets the specifications described in the accompanying Product Datasheet. If it is proven to the satisfaction of GeneCopoeia that the Product fails to meet these specifications, GeneCopoeia will replace the Product. In the event a replacement cannot be provided, GeneCopoeia will provide the purchaser with a refund. This limited warranty shall not extend to anyone other than the original purchaser of the Product. Notice of nonconforming products must be made to GeneCopoeia within 30 days of receipt of the Product. GeneCopoeia s liability is expressly limited to replacement of Product or a refund limited to the actual purchase price. GeneCopoeia s liability does not extend to any damages arising from use or improper use of the Product, or losses associated with the use of additional materials or reagents. This limited warranty is the sole and exclusive warranty. GeneCopoeia does not provide any other warranties of any kind, expressed or implied, including the merchantability or fitness of the Product for a particular purpose. GeneCopoeia is committed to providing our customers with high-quality products. If you should have any questions or concerns about any GeneCopoeia products, please contact us at GeneCopoeia, Inc. GeneCopoeia, Inc Medical Center Drive, Suite 101 Rockville, MD (301) (866) inquiry@genecopoeia.com GeneCopoeia Products are for Research Use Only Copyright 2016 GeneCopoeia, Inc. Trademarks: GeneCopoeia, ExProfile, All-in-One, (GeneCopoeia Inc.); RNAzol RT (Molecular Research Center, Inc.); SYBR (Molecular Probes); icycler iq, MyiQ, iq 5, CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 (Bio-Rad); LightCycler (Roche);Trizol, ABI,ROX,ViiA TM,StepOnePlus TM (Life Technologies). QG