For focused group profiling of human neuroscience ion channels and transporters genes expression

Transcription

1 ExProfile TM Human Neuroscience Ion Channels & Transporters Related Gene qpcr Array For focused group profiling of human neuroscience ion channels and transporters genes expression Cat. No. QG039-A (1 x 96-well plate, Format A) Cat. No. QG039-B (1 x 96-well plate, Format B) Cat. No. QG039-C (1 x 96-well plate, Format C) Cat. No. QG039-D (1 x 96-well plate, Format D) Cat. No. QG039-E (1 x 96-well plate, Format E) Plates available individually or as a set of 6. Each set contains 84 unique gene primer pairs deposited in one 96-well plate. Introduction The ExProfile human neuroscience ion channels and transporters related gene qpcr array profiles the expression of 84 human genes related to neuroscience-related ion channels and transporters. These genes are carefully chosen for their close correlation based on a thorough literature search of peer-reviewed publications, mainly including genes involved in calcium channels, potassium channels, sodium channels, chloride channels, and related transporters. This array allows researchers to study the related genes to gain understanding of their roles in the functioning and characterization of neuronal ion channels and transporters. QG039 plate 01: 84 unique gene PCR primer pairs Shipping and storage condition Shipped at room temperate Stable for at least 6 months when stored at -20 C Array format GeneCopoeia provides five qpcr array formats (A, B, C, D, and E) suitable for use with the following realtime cyclers. Important note: Upon receiving, please check to make sure that the correct array format was ordered to ensure the compatibility with your qpcr instrument. Plate format Instrument provider qpcr instrument model A (96-well) Applied Biosystems 5700, 7000, 7300, 7500, 7700, 7900HT (Standard 96-well block), ViiA TM 7 (Standard 96-well block) B (96-well) Applied Biosystems C (96-well) Bio-Rad Laboratories icycler iq,myiq,iq 5 D (96-well) Bio-Rad Laboratories 7500 (Fast block), 7900HT (Fast block), StepOnePlus TM, ViiA TM 7 (Fast block) CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 E (96-well) Roche Applied Science LightCycler 480 (96-well block) 1

2 Quality control 1. Each pair of primers in the ExProfile gene qpcr array has been experimentally validated to yield a single dissociation curve peak and to generate a single amplicon of the correct size for the targeted gene. 2. The positive PCR controls (PCR) have been verified to amplify a single amplicon of the correct size with Ct values around 20±2. 3. The Spike-in reverse transcription controls (RT) have been verified to amplify a single amplicon of the correct size with Ct values around 20±3. 4. R 2 > 0.99 was observed for high inter/ intra-array reproducibility. Materials required but not provided All-in-One TM First-Strand cdna Synthesis Kit All-in-One TM qpcr Mix Total RNA extraction kit (RNAzol RT RNA extraction reagent is recommended) DNase/RNase free tips, PCR reaction tubes, 1.5 ml microcentrifuge tubes 5 ml and 10 ml graduated pipettes, beakers, flasks, and cylinders 10 μl to1,000μl adjustable single channel micropipettes with disposable tips 5 μl to20μl adjustable multichannel micropipette, disposable tips, and reservoir qpcr instrument, compatible with gene qpcr arrays ordered Array layout Figure1. Illustration of QG039 plate 01 Gene primer pairs: 84 wells (A row to G row) are designated for a real-time PCR assay for genes (see the primer list). HK1-6: Six pre-deposited housekeeping gene (HK1-6) primer pairs, which can be used as endogenous positive controls as well as for array normalization. GDC: Genomic DNA controls, which can be used to specifically detect genomic DNA contamination with a high level of sensitivity. RT: Spike-in reverse transcription controls, which can be used to monitor the efficiency of the RT reactions. These pre-deposited primer pairs specifically amplify the cdna template reversed transcribed from the spike-in control RNA in the sample. PCR: Positive PCR controls, which are used to verify the PCR efficiency by amplifying the predeposited DNA template with its specific pre-deposited primer pairs. 2

3 Gene primer list Plate Position Catalog No. of Primer Accession No. of Gene Symbol QG A01 HQP NM_ KCNQ2 QG A02 HQP NM_ KCNN3 QG A03 HQP NM_ CLCN6 QG A04 HQP NM_ CACNB1 QG A05 HQP NM_ HPRT1 QG A06 HQP NM_ SLC6A4 QG A07 HQP NM_ SLC6A11 QG A08 HQP NM_ SLC2A11 QG A09 HQP NM_ SLC2A1 QG A10 HQP NM_ SLC1A2 QG A11 HQP NM_ SLC1A1 QG A12 HQP NM_ SLC18A1 QG B01 HQP NM_ SCN9A QG B02 HQP NM_ SCN7A QG B03 HQP NM_ SCN5A QG B04 HQP NM_ SCN4A QG B05 HQP NM_ SCN3A QG B06 HQP NM_ SCN2B QG B07 HQP NM_ SCN1B QG B08 HQP NM_ SCN1A QG B09 HQP NM_ KCNS3 QG B10 HQP NM_ KCNS2 QG B11 HQP NM_ KCNS1 QG B12 HQP NM_ KCNQ4 QG C01 HQP NM_ KCNQ3 QG C02 HQP NM_ KCNQ2 QG C03 HQP NM_ KCNQ1 QG C04 HQP NM_ KCNN4 QG C05 HQP NM_ KCNN3 QG C06 HQP NM_ KCNN2 QG C07 HQP NM_ KCNMB1 QG C08 HQP NM_ KCNJ6 QG C09 HQP NM_ KCNJ5 QG C10 HQP NM_ KCNJ4 QG C11 HQP NM_ KCNJ3 QG C12 HQP NM_ KCNJ11 QG D01 HQP NM_ KCNJ1 QG D02 HQP NM_ KCNH2 QG D03 HQP NM_ KCNH1 QG D04 HQP NM_ KCNG1 QG D05 HQP NM_ KCNE1 QG D06 HQP NM_ KCND2 3

4 4 QG D07 HQP NM_ KCND1 QG D08 HQP NM_ KCNC1 QG D09 HQP NM_ KCNB2 QG D10 HQP NM_ KCNB1 QG D11 HQP NM_ KCNA5 QG D12 HQP NM_ KCNA4 QG E01 HQP NM_ KCNA3 QG E02 HQP NM_ CLIC2 QG E03 HQP NM_ CLIC1 QG E04 HQP NM_ CLCN7 QG E05 HQP NM_ CLCN6 QG E06 HQP NM_ CLCN5 QG E07 HQP NM_ CLCN4 QG E08 HQP NM_ CLCN2 QG E09 HQP NM_ CLCN1 QG E10 HQP NM_ CFTR QG E11 HQP NM_ CACNB3 QG E12 HQP NM_ CACNB2 QG F01 HQP NM_ CACNB1 QG F02 HQP NM_ CACNA1S QG F03 HQP NM_ CACNA1C QG F04 HQP NM_ CACNA1B QG F05 HQP NM_ ATP4A QG F06 HQP NM_ ATP1B1 QG F07 HQP NM_ ATP1A1 QG F08 HQP NM_ ACCN1 QG F09 HQP NM_ CACNA1A QG F10 HQP NM_ CLCA1 QG F11 HQP NM_ SCN2A QG F12 HQP NM_ KCNN2 QG G01 HQP NM_ ACCN2 QG G02 HQP NM_ ATP2A1 QG G03 HQP NM_ SLC1A3 QG G04 HQP NM_ GAD1 QG G05 HQP NM_ CACNG2 QG G06 HQP NM_ CLCN3 QG G07 HQP NM_ KCNA1 QG G08 HQP NM_ KCNA2 QG G09 HQP NM_ GPR83 QG G10 HQP NM_ KCNF1 QG G11 HQP NM_ KCNN1 QG G12 HQP NM_ SLC18A2 QG H01 HGDC QG H02 HGDC QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB

5 QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR 5

6 Limited Use License Following terms and conditions apply to use of ExProfile TM Human Neuroscience Ion Channels & Transporters Related Gene qpcr Array (the Product). If the terms and conditions are not acceptable, the Product in its entirety must be returned to GeneCopoeia within 5 calendar days. A limited End-User license is granted to the purchaser of the Product. The Product shall be used by the purchaser for internal research purposes only. The Product is expressly not designed, intended, or warranted for use in humans or for therapeutic or diagnostic use. The Product must not be resold, repackaged or modified for resale, or used to manufacture commercial products or deliver information obtained in service without prior written consent from GeneCopoeia. This Product should be used in accordance with the NIH guidelines developed for recombinant DNA and genetic research. Use of any part of the Product constitutes acceptance of the above terms. Limited Warranty GeneCopoeia warrants that the Product meets the specifications described in the accompanying Product Datasheet. If it is proven to the satisfaction of GeneCopoeia that the Product fails to meet these specifications, GeneCopoeia will replace the Product. In the event a replacement cannot be provided, GeneCopoeia will provide the purchaser with a refund. This limited warranty shall not extend to anyone other than the original purchaser of the Product. Notice of nonconforming products must be made to GeneCopoeia within 30 days of receipt of the Product. GeneCopoeia s liability is expressly limited to replacement of Product or a refund limited to the actual purchase price. GeneCopoeia s liability does not extend to any damages arising from use or improper use of the Product, or losses associated with the use of additional materials or reagents. This limited warranty is the sole and exclusive warranty. GeneCopoeia does not provide any other warranties of any kind, expressed or implied, including the merchantability or fitness of the Product for a particular purpose. GeneCopoeia is committed to providing our customers with high-quality products. If you should have any questions or concerns about any GeneCopoeia products, please contact us at GeneCopoeia, Inc. GeneCopoeia, Inc Medical Center Drive, Suite 101 Rockville, MD (301) (866) inquiry@genecopoeia.com GeneCopoeia Products are for Research Use Only Copyright 2016 GeneCopoeia, Inc. Trademarks: GeneCopoeia, ExProfile, All-in-One, (GeneCopoeia Inc.); RNAzol RT (Molecular Research Center, Inc.); SYBR (Molecular Probes); icycler iq, MyiQ, iq 5, CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 (Bio-Rad); LightCycler (Roche);Trizol, ABI,ROX,ViiA TM,StepOnePlus TM (Life Technologies). QG