OIE Reference Laboratory Reports Activities

Transcription

1 OIE Reference Laboratory Reports Activities Activities in 2014 This report has been submitted : :14:25 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Trichinellosis Address of laboratory: Viale Regina Elena Roma ITALY Tel.: Fax: address: edoardo.pozio@iss.it Website: Name (including Title) of Head of Laboratory (Responsible Official): Edoardo Pozio Name (including Title and Position) of OIE Reference Expert: Edoardo Pozio Which of the following defines your laboratory? Check all that apply: Governmental Research OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally ELISA yes WB 0 59 Direct diagnostic tests Nationally Internationally PCR yes Digestion yes 5 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2014

3 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients Trichinella strains PCR Produced America s Asia and Pacific Middle East Trichinella larvae PCR produced America s Asia and Pacific Middle East Excretory/secretory antigens ELISA and Western blot produced 3 mg 10.5 mg 3 America s Asia and Pacific Middle East Reference sera ELISA and Western blot produced America s Asia and Pacific Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? OIE Reference Laboratory Reports Activities,

4 ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Name of OIE Member Country seeking assistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses BULGARIA May 19 0 CROATIA Several months 10 0 CZECH REPUBLIC several months 5 0 GERMANY March, August 13 SERBIA several months 10 ROMANIA several months 286 SPAIN April 83 SWEDEN Several months 21 HUNGARY Several months 33 UNITED STATES OF AMERICA January 9 ITALY All the months Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? 4 OIE Reference Laboratory Reports Activities, 2014

5 Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided COLOMBIA Control of Trichinella infection in pigs Training course ICELAND Control of Trichinella infection in pigs Forwarding of information by ESTONIA Detection of anti-trichinella IgG in wild boar Forwarding of information by NIGERIA Epidemiological information on Trichinella in Forwarding of information by SOUTH AFRICA Epidemiological information on target animals for Trichinella Forwarding of information by ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Title of the study Duration Purpose of the study Partners (Institutions) Epidemiology of trichinellosis in Vietnam one year Epidemiological survey Parasitology Section, National Centre for Veterinary Diagnosis, Hanoi, Vietnam TRichinella species circulating in Florida six months Epidemiological survey Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University Trichinella in domerstic pigs of Laos one year Epidemiological survey Department of Livestock and Fisheries, Ministry of Agriculture and Forestry, Vientiane, Laos Trichinella populations in Poland one year Identification of molecular markers National Veterinary Research Institute, Puławy ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? OIE Reference Laboratory Reports Activities,

6 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 10 Mohandas N, Pozio E, La Rosa G, Korhonen PK, Young ND, Koehler AV, Hall RS, Sternberg PW, Boag PR, Jex AR, Chang BC, Gasser RB. Mitochondrial genomes of Trichinella species and genotypes - a basis for diagnosis, and systematic and epidemiological explorations. Int J Parasitol Sep 22;44(14): Bruschi F, Bianchi C, Fornaro M, Naccarato G, Menicagli M, Gomez-Morales MA, Pozio E, Pinto B. Matrix metalloproteinase (MMP)-2 and MMP-9 as inflammation markers of Trichinella spiralis and Trichinella pseudospiralis infections in mice. Parasite Immunol Oct;36(10):540-9 Fichi G, Stefanelli S, Pagani A, Luchi S, De Gennaro M, Gómez-Morales MA, Selmi M, Rovai D, Mari M, Fischetti R, Pozio E. Trichinellosis Outbreak Caused by Meat from a Wild Boar Hunted in an Italian Region Considered to be at Negligible Risk for Trichinella. Zoonoses Public Health Aug 7 Conlan JV, Vongxay K, Khamlome B, Gomez-Morales MA, Pozio E, Blacksell SD, Fenwick S, Thompson RC. Patterns and risks of trichinella infection in humans and pigs in northern laos. PLoS Negl Trop Dis Jul 31;8(7):e3034. Vu Thi N, Pozio E, Van De N, Praet N, Pezzotti P, Gabriël S, Claes M, Thuy NT, Dorny P. Anti-Trichinella IgG in ethnic minorities living in Trichinella-endemic areas in northwest Vietnam: study of the predictive value of selected clinical signs and symptoms for the diagnosis of trichinellosis. Acta Trop v;139:93-8. Tolnai Z, Széll Z, Marucci G, Pozio E, Sréter T. Environmental determinants of the spatial distribution of Trichinella britovi and Trichinella spiralis in Hungary. Vet Parasitol Aug 29;204(3-4): Gómez-Morales MA, Ludovisi A, Amati M, Bandino E, Capelli G, Corrias F, Gelmini L, Nardi A, Sacchi C, Cherchi S, Lalle M, Pozio E. Indirect versus direct detection methods of Trichinella spp. infection in wild boar (Sus scrofa). Parasit Vectors Apr 7;7:171. Pozio E. Searching for Trichinella: not all pigs are created equal. Trends Parasitol Jan;30(1):4-11. Boutsini S, Papatsiros VG, Stougiou D, Marucci G, Liandris E, Athanasiou LV, Papadoudis A, Karagiozopoulos E, Bisias A, Pozio E. Emerging Trichinella britovi infections in free ranging pigs of Greece. Vet Parasitol Jan 31;199(3-4): Mirjalali H, Rezaei S, Pozio E, Naddaf SR, Salahi-Moghaddam A, Kia EB, Shahbazi F, Mowlavi G. Trichinella britovi in the jackal Canis aureus from south-west Iran. J Helminthol Dec;88(4): b) International conferences: 2 XIII International Congress of Parasitology (ICOPA XIII), Mexico City, Mexico, 9-15 August, 2014 Trichinosis and other parasitic zoonoses associated with food of animal origin, Pulawy, Poland, 9-10 October, 2014 c) National conferences: 1 28th Congress of the Italian Society of Parasitologists, Rome, June, 2014 d) Other: (Provide website address or link to appropriate information) OIE Reference Laboratory Reports Activities, 2014

7 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 1 b) Seminars: 3 c) Hands-on training courses: 0 d) Internships (>1 month): 1 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a Serbia 1 b Italy 120 d Columbia 1 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO ISO Certificate scan (PDF, JPG, PNG format) pdf pdf 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited Detection of anti-trichinella antibodies in swine serum by indirect ELISA Identification of Trichinella muscle larvae at species level by Multiplex PCR Detection of anti-trichinella antibodies in human serum by indirect ELISA Accreditation body ACCREDIA ACCREDIA ACCREDIA OIE Reference Laboratory Reports Activities,

8 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? 8 OIE Reference Laboratory Reports Activities, 2014

9 te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries Proficiency Testing on the digestion method to detect Trichinella larvae in meat intended for human consumption according to EC Regulation 2075/ Americas Asia and Pacific Middle East Proficiency Testing on Trichinella spp. larva identification at the species level by a molecular method 20 Americas Asia and Pacific Middle East ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: OIE Reference Laboratory Reports Activities,