Intensive skin repair

Transcription

1 Intensive skin repair Temporary version

2 Fast skin healing to erase skin superficial lesions. Erasyal stimulates the three steps of the skin repairing mechanism: control of the inflammatory process activation of the remodeling stimulation of skin restructuring Superficial skin injuries: shaving, acnee, sunburns, scratches,... Erasyal is an active solution of a phosphorylated amino-sugar and of a glycosylated polyphenol both obtained by proprietary green biotechnology processes.

3 Fast skin healing to erase skin superficial lesions. Erasyal stimulates the three steps of the skin repairing mechanism: control of the inflammatory process activation of the remodeling stimulation of skin restructuring Superficial skin injuries: shaving, acnee, sunburns, scratches,... Erasyal 1. Inflammation control by downregulating: TNF-α (heat, pain, redness) IL1-α (immune answer) IL8 (inflammation) 2. Activation of remodeling by acting on: MMP3 and MMP9 Pro-Collagen 1 Erasyal is an active solution of a phosphorylated amino-sugar and of a glycosylated polyphenol both obtained by proprietary green biotechnology processes. 3. Enhancement of repairing by stimulating: Fibronectin and integrin β4 factors Epidermis bud growth

4 Regulation of inflammatory factors (in vitro tests) Protocol: Erasyal was tested for its ability to downregulate three major cytokines involved in skin inflammation during wound healing. IL8 was quantified on keratinocytes extracted from clinical human skin sample incubated with the product for 48h. IL8 (pg/ml/µg proteins) Interleukin 8 release control in IL1-β stimulated keratinocytes -31% * *p<.5 compared to stimulated control, Student s t Test Result: Erasyal downregulates IL8 release by -31%. Induced with IL1ß Control Erasyal - 1%

5 Regulation of inflammatory factors (in vitro tests) Protocol: Erasyal was tested for its ability to downregulate three major cytokines involved in skin inflammation during wound healing. IL1-α was quantified on keratinocytes extracted from clinical human skin sample incubated with the product for 48h. *PMA: Phorbol Myristate Acetate IL1-α (pg/ml/µg proteins) Interleukin1-α release control in PMA* stimulated keratinocytes -49% * *p<.5 compared to stimulated control, Student s t Test Result: Erasyal downregulates IL1-α release by -49%. Induced with PMA Control Erasyal -,5%

6 Regulation of inflammatory factors (in vitro tests) Protocol: Erasyal was tested for its ability to downregulate three major cytokines involved in skin inflammation during wound healing. TNF-α was quantified on keratinocytes extracted from clinical human skin sample incubated with the product for 48h. *PMA: Phorbol Myristate Acetate TNF-α release control in PMA* stimulated keratinocytes TNF-α (pg/ml/µg proteins) % * *p<.1 compared to stimulated control, Student s t Test Result: Erasyal downregulates TNF-α release by -24%. Induced with PMA Control Erasyal - 1.5%

7 Activation of the remodeling (in vitro tests) Protocol: Erasyal was tested for its ability to act on two skin remodeling enzymes. MMP3 and MMP9 were quantified on keratinocytes extracted from clinical human skin sample incubated with the product for 48h. MMP: matrix metalloproteinase Result: Erasyal selectively stimulates MMP3 by 155%, and reduces MMP9 by -42%. MMP3 and MMP9 release in IL1-β stimulated keratinocytes MMP3 (pg/ml/µg proteins) % MMP9 (pg/ml/µg proteins) % Non induced Induced with IL1-β Erasyal - 1.5% Control

8 Activation of the remodeling (in vitro tests) Protocol: Erasyal was tested for its ability to stimulate Pro-collagen 1 synthesis. Its release was quantified on fibroblasts extracted from clinical human skin sample incubated with the product for 48h. Pro-collagen 1 (ng/ml/µg proteins) Pro-collagen 1 release in fibroblasts *p<.5 compared to control, Student s t Test +48% * Result: Erasyal stimulates Procollagen 1 release by 48%. Non induced Induced with Vit. C Erasyal -.1%

9 Erasyal DAY 3 Fibronectin Erasyal 2,5% DAY 7 Integrin β4 Result: Erasyal at 2.5% chronologically induces an overexpression of fibronectin and integrin β4. Control DAY 7 Integrin β4 Protocol: Erasyal was tested for its ability to stimulate fibronectin and integrin β4, two repairing factors. The detection of fibronectin was done on human skin epidermis, 3 days after the creation of an epidermis lesion (ELISA). Integrin β4 was detected on the same principle, but on day 7. DAY 3 Fibronectin Stimulation of skin repairing (ex vivo evaluation)

10 Stimulation of skin repairing (ex vivo evaluation) Protocol: Erasyal and Madecassol (as benchemark) were compared for their ability to act on epidermis bud growth under a lesion. Induced by a UV skin burn. The analysis was done on human skin for 7 days. A 1µm B Growth of epidermis skin bud (µm) Epidermis repairing Result: Erasyal repairs 4 to 7 times quicker than non-treated skin or reference cream. 1µm C 7µm Dashed line: area of epidermis bud growth Non treated skin Madecassol cream Erasyal -.5% Erasyal - 2.5% A: Control - day 7 B: Madecassol cream - day 7 C: Erasyal 2.5% - day 7

11 TECHNICAL INFORMATION INCI: Origin: Preservation: Appearance: Solubility: Dosage: Processing: Intellectual property: Regulatory status: Claims: Glycerin, Disodium Acetyl Glucosamine Phosphate, Caffeyl Glucoside Biotech Preservative free Faint amber solution Water-soluble.5% - 2.5% Can be incorporated in any formula in liquid form at ph below 6.5 and temperature below 45 C Patented JP, KR*, HK, AU*, ASEAN, USA, CA, South-Am., Mid-East, EU Skin repairing, wound healing, stretch marks, anti-aging * Registration pending Ocular irritation: Skin irritation: Mutagenicity: Phototoxicity: Sensibilization: SAFETY ASSESSMENT on NCTC L929 cells - low cytotoxicity at 1% OECD 439 assay - non irritant at 1% Single Patch Test - non irritant at 3% Ames assay - OECD non mutagenic OECD 432 assay - non phototoxic HRIPT assay - non sensitizing at 3%

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