Material Safety Data Sheet: Ultra High Def TM Peroxidase Block

Transcription

1 IVD Data Sheet P2-U-100-HRP; P2-U-20-AUTO-HRP; SAM-P1-U-20-AUTO-HRP 10/7/2014 Ultra High Def TM Polymer HRP Kit CATALOG NO: P2-U- 100 HRP; P2-U-20-AUTO-HRP; SAM-P1-U-20-AUTO-AP PACK SIZE: 100 ml; 20 ml; 10ml Material Safety Data Sheet: Ultra High Def TM Peroxidase Block 1. Identification of the Substance/preparation and of the company/undertaking Identification of the Product: Ultra High Def TM Polymer HRP Kit Catalog No. P2-U-100-HRP; P2-U-20-AUTO-HRP; SAM-P1-U-20-AUTO- AP Manufacture/Supplier Identification: 2. Composition/ Information on Ingredients Dilute solution of Hydrogen Peroxide Chemical Abstracts Registry Number Hazard Identification: The material contains a dilute solution of hydrogen peroxide. Although the concentration of hydrogen peroxide is very low (less than 4% v/v), appropriate care should be taken when handling this material. 4. First Aid Measures Eye Contact: Skin Contact: 5. Firefighting Measures Extinguishing Media: Special Firefighting Procedure: Unusual Fire & Explosions Hazards: 6. Accidental Release Measures Person related precautionary measures: Procedures for cleaning/absorption: Environmental Protection measure: Immediately flush with continuously running water. Immediately wash affected area with soap and water. Rinse mouth with water. Induce vomiting. Immediately seek medical attention and call poison control center. Move to well-ventilated area and seek medical attention if necessary. Extinguish using suitable agents. Wear protective clothing containing self-contained breathing apparatus. Toxic fumes of phosphorus oxides and/or phosphine, carbon monoxide, carbon dioxide, nitrogen oxides and hydrogen chlorides may results from thermal decompositions. Avoid substance contact. Use liquid absorbent material. Clean up affected area. Do not allow to enter sewage system. 7. Handling and Storage Handling: See section 8 Storage: Store at C. Reagent is light sensitive; protect from exposure to light and store in dark environment or opaque bottle. 8. Exposure Controls / Personal Protection Eye protection: Hand protection: Respiratory protection: Ventilation: Other protective equipment: 9. Physical and Chemical Properties Appearance: Wear splash protecting goggles or safety glasses. Wear rubber or vinyl gloves. Required when vapors/aerosols are generated. Use in well-ventilated laboratory. Use lab coat or apron to prevent contact with eyes, skin and clothing. Clear liquid 1

2 Odor: Solubility: 10. Stability and Reactivity Stability: Conditions to be avoided: Substances to be avoided: Hazardous decomposition products: Odorless Miscible with water Stable for 24 months. Refer to product label for expiration. High and prolonged heat. Strong oxidizing agents. No information available 11. Toxicological Information Quantitative data on the toxicity of this product is not available. No toxic effects are to be expected when the product is handled appropriately. 12. Ecological Information No data available. 13. Disposal Considerations Contact professional waste disposal companies. Follow local, state, and federal regulations for disposal. 14. Transport Information Not subject to transport regulations. 15. Regulatory Information US Regulatory Information: SARA listed - No 16. Other Information: The information contained herein is based on the present state of our knowledge. It characterizes the product with regard to appropriate safety precautions. The information provided is without any representation or warranty, express of implied, regarding its accuracy or correctness. The safe usage of this product is the sole responsibility of the user, who is obligated to review this data in the specific context of the intended use and determine applicability. Material Safety Data Sheet: Ultra High Def TM Background Blocker 1. Identification of the Substance/preparation and of the company/undertaking Identification of the Product: Ultra High Def TM Polymer HRP Kit, Catalog No. P2-U-100-HRP; P2-U-20-AUTO-HRP; SAM-P1-U-20-AUTO-AP Manufacture/Supplier Identification: Tel: Composition/ Information on Ingredients Sodium Azide: Chemical Abstracts Registry Number Hazard Identification: The material contains sodium azide as preservative. Although the quantity of sodium azide is very small, appropriate care should be taken when handling this material. The National Institute of Occupational Safety and Health has issued a bulletin citing the potential explosive hazard due to the reaction of sodium azide with copper, lead, brass or solder in the plumbing systems. Sodium azide forms hydrazoic acid in acidic conditions and should be discarded in a large volume of running water to avoid formation of deposits in metal drainage pipes. 4. First Aid Measures Eye Contact: Skin Contact: 5. Firefighting Measures Extinguishing Media: Special Firefighting Procedure: Unusual Fire & Explosions Hazards: 6. Accidental Release Measures Person related precautionary measures: Procedures for cleaning/absorption: Immediately flush with continuously running water. Immediately wash affected area with soap and water. Rinse mouth with water. Induce vomiting. Immediately seek medical attention and call poison control center. Move to well-ventilated area and seek medical attention if necessary. Extinguish using suitable agents. Wear protective clothing containing self-contained breathing apparatus. Toxic fumes of phosphorus oxides and/or phosphine, carbon monoxide, carbon dioxide, nitrogen oxides and hydrogen chlorides may results from thermal decompositions. Avoid substance contact Use liquid absorbent material. Clean up affected area. 2

3 IVD Data Sheet P2-U-100-HRP; P2-U-20-AUTO-HRP; SAM-P1-U-20-AUTO-HRP 10/7/2014 Environmental Protection measure: Do not allow to enter sewage system. 7. Handling and Storage Handling: See section 8 Storage: Store at C. 8. Exposure Controls / Personal Protection Eye protection: Hand protection: Respiratory protection: Ventilation: Other protective equipment: Wear goggles or safety glasses. Wear latex or vinyl gloves. Required when vapors/aerosols are generated. Use in well-ventilated laboratory. Use lab coat or apron to prevent contact with eyes, skin and clothing. 9. Physical and Chemical Properties Appearance: Clear blue liquid Odor: Odorless Solubility: Miscible with water ph: Stability and Reactivity Stability Conditions to be avoided: Substances to be avoided: Hazardous decomposition products: 11. Toxicological Information 12. Ecological Information 13. Disposal Considerations Stable for 18 months. Refer to product label for expiration. Strong and prolonged heat Dimethyl sulfate, acid chlorides, homogenate solvents, metals, acids, lead and copper pluming; forms highly explosive metal azides. No information available Quantitative data on the toxicity of this product is not available. No toxic effects are to be expected when the product is handled appropriately. Preservatives normally are toxic for aquatic organisms. No ecological problems are to be expected when this product is handled and used with due care and attention. Contact professional waste disposal companies. Follow local, state, and federal regulations for disposal. 14. Transport Information Not subject to transport regulations. 15. Regulatory Information This product is not a hazardous material. 16. Other Information: The information contained herein is based on the present state of our knowledge. It characterizes the product with regard to the appropriate safety precautions. It does not represent a guarantee of the properties of the product. Material Safety Data Sheet: Ultra High Def TM Polymer Detection Components 1. Identification of the Substance/preparation and of the company/undertaking Identification of the Product: Ultra High Def TM Polymer HRP Kit Catalog No. P2-U-100-HRP; P2-U-20-AUTO-HRP; SAM-P1-U-20-AUTO-AP Ultra High Def TM Enhancer Ultra High Def TM Universal Polymer (anti-mouse/rabbit) 3

4 Manufacture/Supplier Identification: 2. Composition/ Information on Ingredients Note: In accordance with OSHA regulations and European Union Directives, the products/components listed on this page are not classified as hazardous at ready-to-use concentrations due to their physical and/or chemical nature and/or concentration in solution. Composition disclosure is not required for products/components not classified as hazardous (29CFR ). 3. Hazard Identification The products listed above contain no substances considered to be hazardous to health at the formulated concentrations. Although these reagent products are classified as non-hazardous, good laboratory practices are recommended. Avoid unnecessary contact with reagents and use personal protective equipment during the use of laboratory reagents. Skin contact: May cause mild irritation Eye contact: May cause mild irritation May cause mild irritation to mucous membrane and upper respiratory tract. No harmful effects are expected in amounts likely to be ingested by accident; however, ingestion may cause irritation and malaise. Environment: None identified. 4. Emergency First Aid Measures Eye Contact: Skin Contact: 5. National Fire Protection Agency (NFPA) Rating Fire Hazard/Flash Points: Health Hazard: Reactivity: Specific Hazard: 6. Firefighting Measures Suitable Extinguishing Media Special Firefighting Procedure: Flash Points/Explosion Information: Special Exposure Hazards: 7. Accidental Release Measures Personal Precautions: Procedures for Cleaning/Absorption: Immediately flush with continuously running water, also under the eyelids. Seek medical attention when necessary Immediately wash affected area with plenty of water. Rinse mouth with water. Seek medical attention if symptoms develop. Move to well-ventilated area. Seek medical attention if necessary. Non-flammable No hazard classification Normally stable None identified Use fire extinguishing media appropriate for surrounding materials and fire conditions. Wear self-contained breathing apparatus and protective clothing to prevent contact with eyes and skin N/A (Non-flammable) / LEL: N/A UEL: N/A Combustion or decomposition of materials will not generate hazardous fumes; however, irritating fumes may be produced. Hazardous polymerization will not occur. Personal protective equipment and clothing is recommended during all cleanup procedures. Refer to Section 8 for additional information on exposure controls and personal protective equipment. Avoid physical contact during removal. Absorbent pads or paper towels may be used for small spills. Ventilate area and wash spill site after material pickup is complete. Refer to Section 13 for disposal information. 8. Handling and Storage Handling: Keep containers tightly closed when not in use. See Section 9. Storage: Store at 2-8 C. Keep out of reach of children. 9. Exposure Controls / Personal Protection Permissible Exposure Limit: Precautionary Measures During Use: OSHA TWA/TLV/STEL: None identified Avoid physical contact and prolonged or repeated exposures. Do not breathe vapors if produced. Do not get in eyes, on skin or clothing. Wash thoroughly after handling. Mechanical exhaust is required. Use in well ventilated laboratory. Respiratory protection is highly recommended if irritating vapors are produced during routine usage of this product. Although unlikely, if TWA or other exposure limits are exceeded during the usage of this material, use a NIOSH/MSHA-approved respirator. 4

5 IVD Data Sheet P2-U-100-HRP; P2-U-20-AUTO-HRP; SAM-P1-U-20-AUTO-HRP 10/7/2014 Skin: Eye: Other protective equipment: Environmental Exposure Controls: Follow NIOSH/MSHA and equipment manufacturer s recommendations to determine appropriate equipment choice. Use chemically resistant gloves (e.g. rubber, plastic), lab coat with sleeves or apron. Wear splash-proof chemical safety goggles, glasses or face shield. Eye wash or safety shower Not available. 10. Physical and Chemical Properties Appearance: Liquid Odor: Odorless ph: N/A Flammability (liquid): Non-flammable % Volatiles by Volume: N/A Auto Flammability N/A Water Solubility Soluble Alcohol or Glycerol Solubility N/A 11. Stability and Reactivity Stability: Conditions to Avoid: Materials to Avoid: Hazardous Decomposition Products: Stable for months. Refer to product labels for expiration. Strong and prolonged heat. None identified. None identified. 12. Toxicology Information Polymer Penetration Enhancer / Ultra High Def TM Polymer HRP Label: No LD 50 / LC 50 information is available in relation to normal routes of occupational exposure. Target organ(s): None identified. NOTE: To the best of our knowledge, the chemical, physical and toxicological properties have not been thoroughly investigated at product concentrations. 13. Disposal Considerations Check with local, state, and federal regulations before discharging into sewers or public landfills. 14. Transport Information Not subject to transport regulations. 15. Regulatory Information/Labeling Chemical identification not required on label. These products are not hazardous materials. Material Safety Data Sheets available for professional user on request. 16. Other Information The information contained herein is based on the present state of our knowledge. It characterizes the product with regard to appropriate safety precautions. The information provided is without any representation or warranty, express of implied, regarding its accuracy or correctness. The safe usage of this product is the sole responsibility of the user, who is obligated to review this data in the specific context of the intended use and determine applicability. The MSDS should be retained in an accessible place to employees during all use of this material. Material Safety Data Sheet: Ultra High Def TM DAB Reagent Components 1. Identification of the Substance/Preparation and of the Company/Undertaking Identification of the Product: Ultra High Def TM Polymer HRP Kit Catalog No. P2-U-100-HRP; P2-U-20-AUTO-HRP; SAM-P1-U-20-AUTO-AP Ultra High Def TM DAB Chromogen/Substrate Components Ultra High Def TM DAB Buffer Ultra High Def TM DAB Chromogen Manufacture/Supplier Identification: 5

6 2. Composition/ Information on Ingredients 3-3 Diaminobenzidine: Chemical Abstracts Registry Number Hydrogen Peroxide: Chemical Abstracts Registry Number Hazard Identification: DAB has been classified as a suspected carcinogen and can cause skin irritation upon contact. Avoid contact with clothes and exposed skin. If accidentally contacted, flush with tap water immediately. Follow instructions of local authorities for disposal. 4. First Aid Measures Eye Contact: Skin Contact: 5. Firefighting Measures Extinguishing Media: Special Firefighting Procedure: Unusual Fire & Explosions Hazards: 6. Accidental Release Measures Person related precautionary measures: Procedures for cleaning/absorption: Environmental Protection measure: Immediately flush with continuously running water. Immediately wash affected area with soap and water. Rinse mouth with water. Induce vomiting. Immediately seek medical attention and call poison control center. Move to well-ventilated area. Seek medical attention if necessary. Extinguish using suitable agents. Protective clothing containing self-contained breathing apparatus. Toxic fumes of phosphorus oxides and/or phosphine, carbon monoxide, carbon dioxide, nitrogen oxides and hydrogen chlorides may results from thermal decompositions. Avoid substance contact. Use absorbent material designated for liquids to absorb spill. Do not allow to enter sewage system. 7. Handling and Storage Handling: See section 8 Storage: Store at C. Reagents are light sensitive; protect from exposure to light and store in dark environment or opaque bottle. 8. Exposure Controls / Personal Protection Eye protection: Hand protection: Respiratory protection: Ventilation: Other protective equipments: 9. Physical and Chemical Properties Appearance: Odor: Solubility: ph: 10. Stability and Reactivity Stability: Conditions to be avoided: Substances to be avoided: Hazardous decomposition products: 11. Toxicological Information 12. Ecological Information 13. Disposal Considerations Wear goggles or safety glasses. Wear latex or vinyl gloves. Required when vapors/aerosols are generated. Use in well-ventilated laboratory. Use lab coat/apron to prevent contact with eyes, skin and clothing. Clear and colored liquid Odorless Miscible with water Neutral Stable for 18 months. Refer to product labels for expiration. Strong and prolonged heat Dimethyl sulfate, acid chlorides, homogenate solvents, metals, acids, lead/copper plumbing; forms highly explosive metal azides No information available. Quantitative data on the toxicity of this product is not available. No toxic effects expected when product is handled appropriately. No ecological problems expected when this product is used with care. Do not allow to enter sewage system. Contact professional waste disposal companies. Follow local, state, and federal regulations for disposal. 6

7 IVD Data Sheet P2-U-100-HRP; P2-U-20-AUTO-HRP; SAM-P1-U-20-AUTO-HRP 10/7/ Transport Information 15. Regulatory Information Not subject to transport regulations. US Regulatory Information: SARA listed No 16. Other Information: The information contained herein is based on the present state of our knowledge. It characterizes the product with regard to appropriate safety precautions. The information provided is without any representation or warranty, express of implied, regarding its accuracy or correctness. The safe usage of this product is the sole responsibility of the user, who is obligated to review this data in the specific context of the intended use and determine applicability. Material Safety Data Sheet: Ultra High Def TM UHD Hematoxylin 1. Identification of the Substance/preparation and of the company/undertaking Identification of the Product: Ultra High Def TM Polymer HRP Kit Catalog No. P2-U-100-HRP; P2-U-20-AUTO-HRP; SAM-P1-U-20-AUTO-HRP UHD Hematoxylin Manufacture/Supplier Identification: Tel: Composition/ Information on Ingredients Water Chemical Abstracts Registry Number Glycerin Chemical Abstracts Registry Number Aluminum Sulfate Chemical Abstracts Registry Number Acetic Acid Chemical Abstracts Registry Number Hematoxylin Chemical Abstracts Registry Number Hazard Identification: 4. First Aid Measures Eye Contact: Skin Contact: 5. Firefighting Measures Extinguishing Media: Special Firefighting Procedure: Unusual Fire & Explosions Hazards: This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR ). Immediately flush with continuously running water. Immediately wash affected area with soap and water. Get medical attention if irritation develops. Rinse mouth with water. Induce vomiting. Immediately seek medical attention and call poison control center. Move to well-ventilated area and seek medical attention if necessary. Extinguish using suitable agents. Wear protective clothing containing self-contained breathing apparatus. No unusual fire or explosion hazards expected. 6. Accidental Release Measures Person related precautionary measures: Procedures for cleaning/absorption: Environmental Protection measure: Avoid substance contact. Use liquid absorbent material. Clean up affected area. Do not allow to enter sewage system. 7. Handling and Storage Handling: See section 8 Storage: Store at C. Reagent is light sensitive; protect from exposure to light and store in dark environment or opaque bottle. 7

8 8. Exposure Controls / Personal Protection Eye protection: Hand protection: Respiratory protection: Ventilation: Other protective equipment: 9. Physical and Chemical Properties Appearance: Odor: Solubility: 10. Stability and Reactivity Stability: Conditions to be avoided: Substances to be avoided: Hazardous decomposition products: 11. Toxicological Information 12. Ecological Information Wear splash protecting goggles or safety glasses. Wear rubber or vinyl gloves. Required when vapors/aerosols are generated. Use in well-ventilated laboratory. Use lab coat or apron to prevent contact with eyes, skin and clothing. Purple liquid Slight vinegar Soluble in water Stable under normal temperatures and pressures. Refer to product label for expiration. Fire, static electricity, direct sunlight. Strong oxidizing agents, reducing agents, metals, acids and alkalis. No information available Quantitative data on the toxicity of this product is not available. No toxic effects are to be expected when the product is handled appropriately. No data available. 13. Disposal Considerations Contact professional waste disposal companies. Follow local, state, and federal regulations for disposal. 14. Transport Information Not subject to transport regulations. 15. Regulatory Information US Regulatory Information: SARA listed No 16. Other Information: The information contained herein is based on the present state of our knowledge. It characterizes the product with regard to appropriate safety precautions. The information provided is without any representation or warranty, express of implied, regarding its accuracy or correctness. The safe usage of this product is the sole responsibility of the user, who is obligated to review this data in the specific context of the intended use and determine applicability. INTENDED USE: The Ultra High Def TM Polymer HRP Detection System is a non-biotin, two-step detection system suitable for labeling antigens in formalin-fixed paraffin-embedded tissues and cryostat sections. The Ultra High Def TM Polymer HRP Detection System may also be used with blood smears, cytosmears, and cell preparations. This System has been developed by directly labeling immunoglobulins with enzymes using a proprietary tandem hyperlabelling technology. This ensures consistent and reproducible immunostaining for all types of nuclear, cytoplasmic and membrane antigens in different types of tissues with significantly lower background than detection systems using biotin and avidin conjugates. Ultra High Def TM Polymer HRP Detection System can simultaneously detect both mouse and rabbit antibodies. This System is suitable for use with mouse IgG, IgM and rabbit primary antibodies, both monoclonal and polyclonal. This Detection System has been designed for automated staining on the Thermo 360 IHC platform. The increased sensitivity of the Ultra High Def TM Polymer HRP Detection System enables faster staining procedures without compromising results. FORMAT: Ready to Use INTRODUCTION: Optimal immunostaining not only depends on the specificity of the primary antibody and other immunoreagents but also depends on obtaining a good signal to noise ratio. Binding of an antibody to its epitopes involves van der Waals forces, electrostatic forces and hydrophobic forces. Certain antibodies have tendency to bind loosely and nonspecifically to unrelated epitopes, which can create undesired background staining. In order to remove these nonspecifically bound antibodies, a thorough washing is required after each immunostaining step. Immuno Wash Buffer is specifically designed to remove such loosely bound antibodies effectively and efficiently and to provide a cleaner background staining. KIT CONTENTS: 8

9 IVD Data Sheet P2-U-100-HRP; P2-U-20-AUTO-HRP; SAM-P1-U-20-AUTO-HRP 10/7/2014 Component P2-U-100-HRP P2-U-20-AUTO-HRP SAM-P1-U-20- AUTO-HRP Ultra High Def TM UHD Peroxidase Block 100 ml 20 ml 10 ml Ultra High Def TM Enhancer 100 ml 20 ml 10 ml Ultra High Def TM Background Blocker 100 ml 20 ml 10 ml Ultra High Def TM Polymer HRP (anti-mouse/rabbit) 100 ml 20 ml 10 ml Ultra High Def TM DAB Buffer 5 x 40 ml 5 x 8 ml 5 x 4 ml Ultra High Def TM DAB Substrate Chromogen 10 ml 2 ml 1 ml Ultra High Def TM Hematoxylin 100 ml 20 ml 10 ml PREPARATION OF REAGENT: The reagents are provided in ready-to-use form, with the exception of DAB Preparation of DAB Working Solution: 1. For every 1 ml of the Ultra High Def TM DAB Buffer to a tube or mixing bottle add 1 drop (approximately 20 µl) of Ultra High Def TM DAB Substrate Chromogen to the buffer. Mix thoroughly. 2. The substrate working solution is stable for two weeks refrigerated at 2-8 C. 3. Working solution volume can be scaled up using the same ratio of buffer to Chromogen 4. Dispose of unused DAB/ Substrate working solution in appropriate waste stream according to local, state, and federal regulations. RECOMMENDED STAINING PROTOCOL 1. Paraffin embedded tissue sections must be deparaffinized with xylene or dewaxing agent and rehydrated with a graded series of ethanol and water washes before staining. Follow the standard dewaxing and rehydration protocol used in your lab. 2. The investigator needs to optimize the incubation times for primary antibodies. 3. Each immunostaining run should include known positive and negative controls to assure proper functioning of the staining system and aid in valid interpretation of the results. 4. Consult the primary antibody datasheet for recommended for antigen recovery treatments. Perform epitope recovery pretreatments before starting the staining procedure. PROTOCOL RECOMMENDATIONS: 1. Pretreatment Solution/Protocol: Please refer to the respective primary antibody datasheet for recommended pretreatment solution and protocol. 2. Peroxidase Block: Block for 5 minutes with Ultra High Def TM Peroxidase Block 3. Background Blocker: Incubate for 5 minutes with the Ultra High Def TM Background Blocker 4. Primary Antibody: Please refer to the respective primary antibody datasheet for recommended primary antibody 5. Enhancer: Incubate for 10 minutes with Ultra High Def TM Enhancer 6. Polymer HRP: Incubate for 10 minutes with Ultra High Def TM Polymer HRP (anti-mouse/rabbit) 7. Chromogen: Add 8 drops of DAB Chromogen to 8mls of Ultra High Def TM Substrate Buffer. Incubate tissue for 5 minutes using Ultra High Def TM DAB Substrate Buffer/Chromogen mixture Ultra High Def TM 8. Counterstain: Counterstain with Ultra High Def TM Hematoxylin for 2 minutes TYPICAL CONTROLS: Positive Control: A tissue known to contain the desired antigen, which has yielded positive staining in the past. Negative Controls: Reagent Controls a. Substitute normal non-immune serum from the same host animal as the primary antibody (e.g. if using mouse monoclonal primary antibodies, use mouse non-immune serum). b. Substitute matching host species isotype control for primary antibody c. Use antigen-adsorbed primary antibody (i.e. antibody reagent which has been adsorbed with the target antigen to remove specific antibody) Negative Controls: Tissue control A tissue known to not contain the desired antigen. 9

10 PRECAUTIONS: Specimens, before and after fixation and all materials exposed to them, should be handled as if capable of transmitting infection and disposed of with proper precautions. Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with reagents and specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water. Microbial contamination of reagents may result in an increase in nonspecific staining. Incubation times or temperatures other than those specified may give erroneous results. The user must validate any such change. The MSDS is available upon request. LIMITATIONS AND WARRANTIES: There are no warranties, expressed or implied, which extend beyond this description. Diagnostic BioSystems is not liable for property damage, personal injury, or economic loss caused by this product. STORAGE AND STABILITY: Store at 2ºC to 8ºC. Do not use after expiration date printed on vial. If reagents are stored under conditions other than those specified in the package insert, they must be verified by the user. Diluted reagents should be used promptly; any remaining reagent should be stored at 2ºC to 8ºC. Stability is months (see expiration date on reagent bottles) TROUBLESHOOTING: Follow the antibody specific protocol recommendations according to data sheet provided. If atypical results occur, contact Statlab Medical Products Technical Support at