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1 رزو : : : : ( Ph.D) : : : Degree Discipline Date Institution. B.Sc Microbiology Isfahan University M.Sc Medical Bacteriology Tehran university PhD Medical Biotechnology Pasteur Institute of Iran : / : 1380 / : :

2 : chimeric DNA vaccination : : 1380 ( ) : Shahrokhi, N,. Jafari, A. Bakayev, V.V. Schirmbeck, R. Reimann, J. Buzari, S Construction of hybrid hepatitis B surface antigen carrying heat-stable enterotoxin of Escherichia coli and its expression in mammalian cell line. Iranian Biomedical Journal 6(2&3), Shahrokhi, N,. Buzari S,.jafari, A, Study of Immune Response of Mice to Chimeric Hepatitis B Core/ a Determinant Antigen. The 11 th Iranian Congress on Infectious diseases and Tropical Medicine. Arzanloo, M. Rezaee, A. Shahrokhi, N. Zavaran Hosseini, A. Ahangarzadeh rezaee, M Amplification and Cloning of ctbb gene of Vibrio cholera 565B in Saccharomyces cerevisiae Shuttle Vector. The 7 th Iranian congress og Microbiology. Semnan Jun 2004 Shahrokhi, N. Bakayev, V.V, Quality controls in molecular diagnostic methods. 12 th Iranian congress on Infectious diseases and tropical Medicine. Tehran, Jun 2004.

3 Jamshidi, A,. Shahrokhi, N. Oloomi, M. Dashti, A. Buzari, S Cloning and expression of fimbrial Subunit of Entroaggrigative Escherichia coli The 4 th national biotechnology Congress Islamic republic Of Iran. Kerman august Shahrokhi, N,. Buzari S,. Reimann J, Coexpression of Hepatitis B Surface and Core Antigen Using a Bidirectional vector. The 4 th national biotechnology Congress Islamic republic Of Iran. Kerman august Arzanlou, M. Rezae, A. Shahrokhi, N. Zavaran Hossini, A. Yasuda, Y. Tochikubo Y. Ahangarzadeh Rezaee Expression of cholera toxin B subunit in Saccharornyces cerevisiae. Annals of Microbiol, 55 (2) Habibi Roudkenari, M. Bouzari, S. Oloomi, M. Jafari, A. Shahrokhi, N. Shokrgozar, MA Expression of a Chimeric Protein Containing the Catalytic Domain of Shiga-Like Toxin and Human Granulocyte Macrophage Colony-Stimulating Factor (hgm-csf) in Escherichia coli and Its Recognition by Reciprocal Antibodies. Iranian Biomedical Journal 9 (4): Dashti Khavidak, A,. Jafari, A,. Shahrokhi, N. Bouzari, S Cloning and Expression of Gene encoding Adhesin of enteroaggregative Escherichia coli (ETEC). The 14 th Iranian Congress on Infectious diseases and Tropical Medicine. Tehran. IRAN. Dashti Khavidak, A,. Jafari, A,. Oloomi, M,. Shahrokhi, N. Bouzari, S Detection of Intimin β gene among diarhoeagenic Escherichia coli. The 14 th Iranian Congress on Infectious diseases and Tropical Medicine. Tehran. IRAN. Shahrokhi, N,. Buzari S,.Jafari, A, Effect of Poly-Glycin Linker on the Presentation of forign epitops. 13 th International Symposium on HIV & Emerging Infectious diseases Toulon, France, Jun Shahrokhi, N. Bouzari, S. Jafari, A Priming hepatitis B surface (HBsAg)- and core antigen (HBcAg)-specific immune responses by chimeric, HBcAg with a HBsAg a determinant. Iranian Biomedical Journal 10 (2): Shahrokhi, N., Hajia M., Amir-zargar A.A., Farzane-Khah M., Ghoreishi M., Saraf-nejad A Comparison of HBV DNA and Serology markers in patients with clinical evidence of hepatitis B refer to Noor Medical Lab Lab & Medicine Symposium, Tehran, IRAN Hajia, M. Shahrokhi, N. Amirzargar, AA. Farzanehkhah, M. Biglari, S. Ghorishy, M. Sarafnejad, A Prevalence of Hepatitis C virus among Out-Patients of a Private Laboratory in Tehran. Iranian J Publ Health, Vol. 36, No. 1, 2007, pp Haji, M. Amirzargar, AA. Khedmat, H. Shahrokhi, N. Farzanehkhah, M. Ghorishi, SM. Biglari, S. Salehinodeh, AK. Sarafnejad, A Genotyping Pattern among Iranian HCV Positive Patients. Iranian J Publ Health, Vol. 39, No. 2, 2010, pp Saied Bouzari, Nader Shahrokhi, Amir Dashti, Ali-Reza Janani, Anis Jafari Construction and evaluation of chimeric heat-labile toxin B subunit and N-terminal (1-75) fragment of colonization factor antigen I gene of enterotoxigenic Escherichia coli. Ann Microbiology, (2010) 60: Meysam Sarshar, Abbas Doosti, Anis Jafari, Nader Shahrokhi,.(2010) "Evaluation of oligonucleotide microarray for detection of foodborne pathogenic bacteria". J Microb. World. Vol 2, No: 2,

4 Nader Shahrokhi, Masoud Hajia, Maysam Shahrokhi, Foroozan Abbasi, Mohamad Farzanehkhah Development of a test system for simultaneous detection of HIV RNA and Hepatitis C virus (HCV) and DNA of Hepatitis B virus (HBV) in blood samples. Retrovirology 2010, 7(Suppl 1):P169. M. Sarshar, A. Jafari, A. Doosti, N. Shahrokhi (2010). Use of 23S rdna gene diversity for the discrimination of foodborne pathogenic bacteria by oligonucleotide microarrays. INT J INFECT DIS, vol. 14, pp. S13-S13. Nader Shahrokhi, Saeid Bouzari, Anis Jafari, (2011). Comparison of virulence markers and antibiotic resistance in enterotoxigenic Escherichia coli isolated ten years apart in Tehran. J Infect Dev Ctries 2011; 5(4): Meysam Sarshar, Ahmad Hassani, Nader Shahrokhi,. (2012) "EVALUATION OF VARIOUS PROBES BASED ON 23S RDNA GENE FOR THE DETECTION OF ESCHERICHIA COLI AND SALMONELLA ENTERICA". EKMUD Congress. (Infectious Diseases and Microbiology), 9-12 May Istanbul, Turkey. Tavafi H, Olia P, Shahrokhi N. (2012). "Prevalence of Toxigenic C. difficile (A - B + ) in children with cancer". Iran J Infect Dis Trop. Vol 17, (57) Hasani A, Shahrokhi N, Khezer-doost S, Sarshar M, Takroosta N, Noroozi J,. (2012). "Detection of Ureaplasma Urealyticum and Mycoplasma hominis in Clinical isolates from Women with Genital Tract Infection by PCR". Iran J Infect Dis Trop. (In Press). Meysam Sarshar و Reza Ranjbar, Nader Shahrokhi, Noorkhoda Sadeghifard, Ahmad Hassani, Zohreh Nasrabadi, Fatemeh Poorali. (2013). Detection of Escherichia Coli, Salmonella Enterica and Shigella Dysenteriae by Analysis of 23S Ribosomal DNA Gene. Journal of Isfahan Medical School. Vol. 30, No :(Patents) (Iran patent No: ) (Iran patent No: ). DNA.1.2 : (STD).1.2

5 : : : o 1- Evaluation of virulence markers and antibiotic resistance in clinical isolates of enterotoxigenic Escherichia coli. 2- Study of gene coding adhesive factor in EAEC for DNA vaccination. 3- Application of Oligonucleotide array method for the rapid detection of foodborne pathogens. 4- Rapid detection of Toxigenic C. difficile in children with cancer. 5- Application of Oligonucleotide Microarray for detection of bacterial pathogens causing sexually transmitted diseases. 6- Use of zip nucleic acid probes and fluorescence hybridization method for simultaneous detection of urogenital pathogens. :PhD : o 1. Production and evaluation of immune response against recombinant saccharomyces cerevisiae expressing B subunit of Vibrio cholereae enterptoxin in animal model. 2. Construction of Chimeric protein containing Shiga Toxin-GMCSF