Annual meeting as a 2 day symposium This year with several European speakers

Transcription

1 More than 140 participants. Please use the opportunity for networking. Presentations are covered and with abstracts and selected PDFs of presentations. Annual meeting as a 2 day symposium This year with several European speakers

2 Program 10th November News from 3R centres and activities DK: European engagement, survey, teaching, 3R prize, grants NC3R: More than 10 years 20 employed, supported 238 major awards, active interaction with researchers, SMEs, industry Acute tox study with impact on ICH M3 guideline, Data sharing, Arrive, Crack it, ExpDesignerAssistant. Discussion about inclusion of animal welfare organisations

3 Program 10th November News from 3R centres and activities Århus: Symposium on Metaanalysis November Novo Nordic: 4 persons engaged in new 3R unit focusing on internal projects and internal collaborations. First in the world. Established on ethical grounds.

4 Program 10th November News from 3R centres and activities Presentation of last years 3R projects supported by the 3R centre: Polyclonal antibodies in chicken Otto Kalliokoski In vitro human skin model Mette Elena Skindersøe In vitro model to predict lung toxicity Søren Thor Larsen

5 Home Teaching material for high school from 3R centre

6 Resource room group assignments (detailed and deeper understanding)

7 The 3Rs: on animal scientists perceptions, awareness and practices Overall aim: to improve the basis for further implementation of the 3Rs in Denmark and establish a baseline for assessing future implementation Research questions 1. What is the level of awareness and knowledge about the 3Rs among Danish animal scientists? 2. To what extend are the 3Rs implemented in practice? 3. What are the barriers for further implementation?

8 Summing-up the obstacles and opportunities Viewed as a whole, most welcome the 3Rs and see no obstacles The publicly employed see more obstacles than the privately employed Obstacles are mainly technical/ innovational Increased data sharing is seen as an important means to achieve an overall reduction of the number of animals used Replacement generally not considered as feasible as the other Rs and seems to be the greatest challenge progress could be achieved by education

9 Preliminary findings Number of animals used ind ex Offentlig 38 % (1,2%) Privat 62 % (6.1%) I alt 100 % (4%)

10 Preliminary findings Types of investigations selected years 70, ,0 % 50,0 40,0 30,0 20,0 10,0 0,0 Biological investigations Medical, odontological and veterinary medical investigations Quality control - Quality control - medical, veterinary odontologic Toxocological safety assessments Diagnostic Uddannelse og undervisning (T2_8) Andet (T2_9)

11 Animal use for science in Europe. Daneshian M, Busquet F, Hartung T, Leist M. ALTEX. 2015;32(4):

12 Animal use for science in Europe. Daneshian M, Busquet F, Hartung T, Leist M. ALTEX. 2015;32(4):

13 Animal use for science in Europe. Daneshian M, Busquet F, Hartung T, Leist M. ALTEX. 2015;32(4):

14 Posters presented Al-Malahmeh et al, Wagening University: Physiologically based kinetic modelling of bioactivation of myristirum facilitating risk assessment Yishi Huang et al, Dansih Nanosafe Centre: The constrained drop surfactometer as a toll for toxicological assessment of impregnation spray products Alayjlouni AM et al, Wagening: Mode of action based risk assessment of the botanical food-borne alkenylbenzene apiol from parsley using physiologically based kinetic (PBK) modelling and read across to safrole

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16 Novo Nordisk

17 However.. Simply meeting the legislate requirements will not ensure appropriate welfare, care and use practices Requires commitment Commitment enabled through institutional support Institutional support facilitated via regulatory support

18 Culture of Challenge Challenge the path for discovery Researcher Right questions? Right disciplines? Right models? Beyond obvious? Refinement..

19 Conclusions Legal framework, partners and networks in place to strive strategically for new alternative approaches take part From aspiration to practical, continued implementation of the Three Rs Three Rs is everyone s responsibility Time to roll out Culture of Challenge - for the benefit of science and animals

20 Definition of QSAR: Quantitative Structure-Activity Relationship A QSAR is a mathematical model (often a statistical correlation) relating one or more parameters derived from chemical structure to a property or activity, e.g. a toxicological endpoint See e.g. EU chemicals legislation, REACH, guidance R.6: QSARs and grouping of chemicals for more information requirements_r6_en.pdf

21 New Danish QSAR predictions database

22 Stem cells as tools to address the 3Rs Stem cells Animal models Simple in vitro assays Organoids/3D models Whole organ models Microfludic systems Organs-on-chip Complexity

23 CAAT 2.1 a vision and a strategy led by Thomas Hartung

24 Hannes remarks Awareness internally and externally of lab animals welfare through education, communication and dialogie Review of a lot of applications for permits and of protocols of animal experimentation Stop for the use of many thousands of mice, rats, rabbits for batch control of efficacy and purity of insulins glucagons, growth hrne and FVII Stop for use of thousands of mice for production of monoclonal antibodies by the ascites method Inroduction and use of better anestesia and analgesia Improvement of housing and environmental enrichment My 1 priority as lab animal vet has been to be available for the animals, the animal technicians and the scientists

25 Announcement of Workshops August 2016 at Panum, University of Copenhagen with 3R Bridging genomics, human environmental health risk assessment and the 3Rs in animal science to be organized by the Nordic Environmental Mutagen Societies (NordEMS) and adhered to program of the European Environmental Mutagen Societies (EEMS), and European Concensus Platform of Alternatives (ECOPA) in August 2016 in Copenhagen. Organising committee:lisbeth E. Knudsen, University of Copenhagen, Denmark (chair) Jørn A. Holme, Norwegian Institute of Public Health, Head of Norwegian Society of Pharmacology and Toxicology (NSFT) Margareta Törnqvist, Stockholm University, Sweden Tuula Heinonen, president of FICAM and SSCT, Finland Kristín Ólafsdóttir, University of Iceland