Catarina Andreia Ricacho Gouveia

Transcription

1 Catarina Andreia Ricacho Gouveia Personal Data Gender: female Birth: 25 of September, 1990 Nationality: Portuguese Adress: Praceta José Domingos dos Santos nº 6 4º Drt, , Lavradio, Portugal Mobile phone: Phone: catarina.a.gouveia@gmail.com Career Aim I aim for a career in Microbiology. I m interested in either research studies in Microbial Ecology and Population Ecology, or in more applied microbiology in industrial areas and services. Such as, food production and water, soil and food quality control. In research my main interests are focused in nutrient cycling and their use efficiency. I m interested particularly in nitrogen and phosphorus and plant microorganism interaction, with potential focus on agricultural practices. Professional experience Laboratory Work February 2013 to December 2014 (2 years) - Research assistant at CBA (Centre of Enviromental Biology). Under the orientation of Professor Cristina Cruz, in the scope of the FCT funded project PTDC/BIA-ECS/122214/ In-Nitro: conceptualizing the effects of increased nitrogen availability in a Mediterranean ecosystem. Main activities ranged from soil sampling, preparation and analysis. Soil chemical, physical, enzymatic and microbiological analysis. Microbial diversity analysis with culture-dependent methods. Growth medium preparation and sterilization. Analysis of functional fungi and bacterial groups diversity and their activities Phosphorus solubilisation, Nitrogen cycling (N fixation, Nitrification and Denitrification). March to April 2013 (2 months) - Research assistant at University of Helsinki, Faculty of Agriculture and Forestry. Under the supervision of Professor Sari Timonen, in the scope of a short term scientific mission funded by COST Action FP Belowground Carbon Turnover in European Forests. Main activities were preparation of samples for Illumina pyrosequencing. Preparation of fungal samples for Singer sequencing. Basic bioinformatics analysis with Geneious for Primer design. September 2011 to February 2012 (6 months) Internship at ITQB (Instituto de Tecnologia Quimica e Biológica). Under the supervision of Professor Sérgio Filipe in the scope of a Master subject (Initiation to Research in Microbiology). Main activities included genomic DNA and plasmid extraction. Induction of competence (in order to receive plasmids) in Gram positive bacteria. Bacterial cloning with reporter genes (GFP) using with non-recombinant plasmids,

2 determination of growth curves and observation under fluorescence microscopy. All work was done using Streptococcus pneumonia as a model organism. August to September 2010 (2 months) Intership at a Public Health Safety Laboratory in Setúbal, under the supervision of Dr. Paula Soares. Main activities included chemical and biological water analysis. Collection and preparation of water samples from public and private water supplies, pools and beaches. Identification of Salmonella Sp., Escherichia coli, Enteroccocus and other coliforms through kits. Identification and quantification of Legionella sp. through real-time PCR. Elaboration of reports and data base update. Projects: : "In-nitro: conceptualizing the effects of increased nitrogen availability in a Mediterranean ecosystem". PTDC/BIA-ECS/122214/2010. Participant Research assistant : "Rhizospheric microbial consortia to increase nutrient use efficiency. A tool to be used in intensive farm systems". PTDC/AGRO-PRO/115888/2009. Participant Volunteer : "Estudio de la respuesta del metabolismo del nitrógeno en plantas de interés agronómico ante la nutrición amoniacal através de su composición de isotopos estables (15N/14N)" within the second ANABASID Programme. Post-doctoral mobility grant to Dr. Idoia Ariz Arnedo (DNI T). Participant Volunteer : "Cultivation of Pineapple in the Azores: Research, Development and Application of Technologies and Practices to Improve Competitiveness and Quality of Production". RAAFDR FEDER Participant Volunteer Professional experience Teaching and Orientation Assisted Professor Cristina Cruz and Professor Margarida Ramos teaching two classes on Ammonia Transporters in yeasts within the subject Cellular and Molecular Physiology from the Master degree in Human Molecular Biology and Genetics and Molecular Biology at the Department of Plant Biology and Animal Biology, Faculty of Sciences from the University of Lisbon (Portugal) Supervision of a 3-month internship of an Erasmus student within the Erasmus Training Programme; Nitrogen Cycling and mineralization (Portugal) Assisted Professor Margarida Ramos teaching four classes on Antibiotic Resistance in Biofilms within the subject Physiology from the degree in Biochemistry at the Department of Chemistry and Biochemistry, Faculty of Sciences from the University of Lisbon (Portugal) Assisted Professor Cristina Cruz and Professor Margarida Ramos teaching two classes on Ammonia Transporters in yeasts within the subject Cellular and Molecular Physiology from the Master degree in Human Molecular Biology and Genetics and Molecular Biology at the Department of Plant Biology and Animal Biology, Faculty of Sciences from the University of Lisbon (Portugal).

3 2013 Assisted Professor Manuela Carolino teaching two classes on N cycling within the subject Microbiology laboratory II from the Master degree in Applied Microbiology at the Department of Plant Biology, Faculty of Sciences from the University of Lisbon (Portugal). Conference Proceedings Oral presentations Gouveia C, Ulm F, Dias T, Carolino M & Cruz C (2014). In the quest for phosphorus: the role of P solubilizing bacteria in helping plants accessing P in an N-limited Mediterranean ecosystem. XV Simpósio Luso-Espanhol de Nutrição Mineral das Plantas - NutriPLANTA 2014, 6-8 December, Lisbon (Portugal). Ulm F, Gouveia C, Dias T & Cruz C (2014). Nitrogen fertilization induces P depletion and shifts P acquisition from the root surface of Cistus ladanifer into the surrounding bulk soil. XV Simpósio Luso-Espanhol de Nutrição Mineral das Plantas - NutriPLANTA 2014, 6-8 December, Lisbon (Portugal). Dias T, Gouveia C, Ulm F, Chaves S, Tenreiro R, Martins-Loução MA & Cruz C (2014). Soil receives, soil reports: soil bacterial communities as rapporteurs of integrated ecosystem responses to increased Nitrogen availability. 18th Nitrogen Workshop, 30 June - 3 July, Lisbon (Portugal). Conference Proceedings Poster presentations Gouveia C, Cruz C, Chaves S, Dias T & Carolino M (2014). Effects of N addition on Mediterranean soil s nitrifier populations: Ammonia oxidizing bacteria as disturbance indicators. 18 th Nitrogen Workshop, 30 June - 3 July, Lisbon (Portugal). Ulm F, Gouveia C, Dias T & Cruz C (2014). Enhanced nitrogen availability has an impact on extracellular enzyme activity in the rhizoplane of Cistus ladanifer but not in the surrounding soil. 18 th Nitrogen Workshop, 30 June - 3 July, Lisbon (Portugal). Ulm F, Gouveia C, Dias T & Cruz C (2014). N enrichment decouples the relation between N and P cycling in the rhizoplane but not in the bulk soil: evidence from an N-manipulation field experiment in a Mediterranean ecosystem. European Geoscience Union General Assembly, 27 April - 2 May, Vienna (Austria). Carolino M, Lima C, Gouveia C, Correia P, Carvalho L, Dias T, Cruz C (2012). Caracterização da comunidade microbiológica associada aos substratos utilizados na cultura do ananás. V Congresso Ibérico da Ciência do Solo September, Açores (Portugal). Education December 2013 Received the degree of Master in Applied Microbiology at Faculty of Sciences, University of Lisbon with a final grade of 16 (of 20). Thesis project was entitled Nitrification Control. How does the Natural ecosystem do it? and done under the supervision of Doctor Manuela Carolino. September 2011 Receive the degree in Biology, branch on Genetics and Molecular Biology at Faculty of Sciences, University of Lisbon, with a final grade of 14 (of 20).

4 July 2009 Summer school on the scope of Biological Anthropology I Introduction to the Analysis of the Human Skeleton at Faculty of Social and Human Sciences, New University of Lisbon. July Summer school on the scope of Biological Anthropology II Introduction to the Analysis of Paleopathogenic Diseases at Faculty of Social and Human Sciences, New University of Lisbon. Language Skills Mother tongue: Portuguese Understanding Speaking Writing Language Spoken Spoken Listening Reading interaction production English C1 C1 B2 B2 B1 Spanish B2 B2 A2 A2 A1 French B1 B1 A1 A1 A1 Levels: A1/2: Basic user - B1/2: Independent user - C1/2 Proficient user Common European Framework of Reference for Languages Professional Skills Microbiology Techniques: Soil and water sample preparation. Sterilization methods (dry, humid, UV). Media preparation and plating. Isolating, cultivating and maintaining bacterial strains. Identification of bacterial strains (molecular by 16S sequencing or physiological by BBL galleries).growth curve determination. Activity determination and quantification. Molecular techniques: Genomic DNA extraction from Gram positive Bacteria, Gram Negative Bacteria and Fungi. Plasmid DNA extraction. PCR, PCR-RFLP, nested PCR. Induction of Competence in Streptococcus pneumonia and Escherichia coli. Cloning with non-recombinant plasmids and reporter genes. Protein quantification. DNA quantification. Chemical analysis techniques: Ammonia, nitrate, nitrite and phosphorus quantification by colorimetric methods. Statistical analysis: SPSS (basic) Informatics skills: Office 2013 (Word, Excel, Powerpoint) Driving License B category Other skills (hobbies) Baker in the free times

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