Get ahead in your research with QIAGEN' s Total RNA Discovery bundle

Transcription

1 Sample to Insight et ahead in your research with QIN' s Total RN iscovery bundle et ahead of the competition with QIN's lab validated PR array technology. uy from each of the above workflow steps to enjoy % savings today!* * This offer is valid until th November. The reduction of the price is based on the list price valid at the time of the promotion and is only valid for orders during the time period and limited lo the amounts mentioned. Offer may not be combined with any other promotions. Void where prohibited. It is the customer's responsibility to ensure that acceptance of this offer will not violate any internal policies of their organization. Pricing is in New Zealand ollars, excluding ST and local freight. Offer only valid in New Zealand. info@bio-strategy.com 00

2 Total RN iscovery Simultaneously profile mrn, mirn and lncrn using a simple, complete workflow Introducing QIN Total RN iscovery with RT 2 Profiler and mirn PR rrays The study of RN has evolved from the simplicity of the central dogma of molecular biology. There are now multiple known noncoding RN species that directly regulate gene expression. To truly understand gene expression, exploring regulatory RN, such as mirn and lncrn, is key. Our unified workflow, combining RT 2 and systems, can be used to simultaneously quantify all three species. With QIN Total RN iscovery, you won t miss a molecular signature or biomarker. Sample to Insight

3 mirn II RT Kit Premp PR Kit mirn PR rray Sample isolation P serum/plasma cell line exosomes mirneasy RT reaction Optional preamplification for low sample inputs PR array ata analysis using enelobe ata nalysis enter mrn lncrn RT 2 irst Strand Kit RT 2 Premp cn Synthesis Kit RT 2 Profiler/ lncrn PR rray et more results from less sample using mirneasy Purification of high-quality total RN, including mrn, mirn and lncrn, is a prerequisite for successful RN quantification experiments. hoose mirneasy Kits from total RN purification from cells, tissues, P tissues and all biofluids including serum, plasma, S, urine and cell culture media. mirneasy P: The mirneasy P Kit provides special lysis and incubation conditions to reverse formalin crosslinking of RN and efficiently purify RN while avoiding further RN degradation. mirneasy Serum/Plasma: The mirneasy Serum/Plasma Kit enables total RN purification from human and animal serum, plasma or other biofluids. enefits of real-time PR for RN quantification and verification qpr remains the gold standard for quantification of RN. qpr provides flexibility and speed for time-critical assays. or NS users, verify and focus your results using our qpr systems for both annotated and novel mrn, mirn and lncrn. Sensitivity: hemistry and assays designed to efficiently detect and quantify even the rarest target. Specificity: Our proprietary algorithms and bench-verification assure on-target amplification from a single assay to a full array. ynamic range: Whether you have unlimited or precious amounts of total RN, robust or rare targets, one assay or thousands, our tailored sample to insight workflows enable detection across the entire spectrum. Why Total RN iscovery using QIN PR rrays? ontent: Unmatched catalog of expertly curated assays in ready-to-use qpr plates for targeting pathways, diseases and application-specific targets in mrn, lncrn and mirn. ontrols: With integrated sample isolation, reverse transcription and qpr controls, you will be able to monitor for any variables that affect qpr performance and may compromise data analysis. ustom: QIN offers unparalleled capabilites to customize arrays to fit your needs. ustom arrays can be designed for both mrn and lncrn together, as well as customized arrays for your mirn of interest.

4 vailable mirn PR rrays rray omplete mirnome Species, dog, rhesus macaque miinder, dog, rhesus macaque rain ancer reast ancer ancer Pathwayinder H e e SN1 SN2 SN3 SN4 SN5 SN6 mirt mirt ell ifferentiation & evelopment Immunopathology Inflammatory Response & utoimmunity Neurological evelopment & isease Ovarian ancer Serum & Plasma ustom rray, dog, rhesus macaque, and other species. elegans mir- Primer ssay snorn/snrn PR ontrols Reverse Positive transcription PR control control mirn PR rray layout. mirn PR rrays provide Primer ssays for mirns, as well as controls for data normalization, reverse transcription and PR. ata normalization controls include 6 PR-ontrols and a primer assay for. elegans mir-. The cel-mir- assay detects the mirneasy Serum/ Plasma Spike-in ontrol, which can be added during biofluid RN isolation to monitor for differential RN recovery. Rotor-isc 0 and 4-well formats are also available. mirn PR rrays ontent: The most comprehensive catalog of assays and arrays available for all annotated species. Targeted arrays: targeted arrays offer cataloged options focused on disease- and pathway-specific mirn for human, mouse, dog, rhesus macaque and rat. mirnome: offers the mirnome for profiling of over 00 mirn, offering specificity for each transcript, and driving biomarker discovery with novel data. ustomizable: arrays are fully customizable to fit your personalized needs. Simply build your list and receive the array. RT 2 Profiler/lncRN PR rrays RT 2 Profiler and RT 2 lncrn PR rrays are highly standardized, reliable and sensitive gene expression profiling tools for analyzing focused panels of genes involved in signal transduction, biological processes or disease research pathways, using real-time PR. Simplicity: The simplicity of RT 2 Profiler PR rrays makes routine expression profiling practical in any research laboratory with a real-time instrument. Performance: RT 2 Profiler PR rrays have the sensitivity, reproducibility, specificity and reliability to accurately profile multiple genes simultaneously in 96- and 4-well plate, 0-well disc, and 96 x 96 chip formats. Relevance: RT 2 Profiler PR rrays focus on profiling the genes relevant to the pathways or disease states important to your research.

5 atalog RT 2 PR rray plate layout for 96- and 4- well qpr instruments H HK1 HK2 HK3 HK4 HK5 RT ene-specific assays Housekeeping genes enomic N control ach well in an RT 2 Profiler PR rray measures the expression of a gene related to a pathway or disease state. typical 96-well and 4-well format is shown. This is also available in a 0-well disc for the Rotor-ene Q, and x or 96 x 96 chip format for the luidigm iomark. RT RT Reverse transcription controls Positive PR controls H I J K L M N O R R R R R R R R R R RT RT RT RT RT RT P R R R R R R R R R R RT RT RT RT RT RT Reference genes enomic N controls Reverse transcription controls Positive PR controls Popular RT 2 Profiler/lncRN PR rrays ngiogenesis poptosis utophagy reast ancer and strogen Receptor Signaling ancer Pathwayinder ell ycle hemokines and Receptors iabetes N amage Signaling Pathway rug Metabolism /P Signaling Pathway pigenetic hromatin Modification nzymes pithelial to Mesenchymal Transition xtracellular Matrix and dhesion Molecules PR Signaling Pathway rowth actors Heat Shock Proteins Hedgehog Signaling Pathway Hematopoietic Stem ells & Hematopoesis Hepatoxicity Inflammatory ytokines and Receptors Innate and daptive Immune Response Interferon α,β Response JK/STT Signaling MP Kinase Signaling Pathway Mitochondrial nergy Metabolism Nκ Signaling Pathway Nephrotoxicity Oxidative Stress and ntioxidant efense p Signaling Pathway Wnt Signaling Pathway See how you can benefit from Total RN iscovery at Trademarks: QIN, enelobe,, Rotor-isc, Rotor-ene, Sample to Insight (QIN roup). Registered names, trademarks, etc, used in this document, even when not specifically marked as such, are not to be considered unprotected by law. 9 PROM--0 / QIN, all rights reserved. Ordering Technical Support support.qiagen.com Website