313, Via Santa Maria, Quarto (NA) January 2015 to today

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1 E U R O P E A N C U R R I C U L U M V I T A E F O R M A T PERSONAL INFORMATION Name ANDREA PASQUALINI Address Telephone Fax 313, Via Santa Maria, Quarto (NA) andrealestrisce@hotmail.it Nationality Italian Date of birth 16/11/1983 WORK EXPERIENCE Dates (from to) Dates (from to) January 2015 to today C.N.R. (National Research Center) at the Institute for Sustainable Plant Protection. Research Research assistant Employed in a Co.Co.Co. Monitoring of viral and viroidal diseases in solanaceous vegetables and ornamentals. Analysis of plant samples by serological techniques (ELISA) and molecular (RT-PCR). Cloning of viral and viroidal amplicons and sequence analysis. Periodic processing of technical and scientific reports on its activity. Entering data monitoring platform of regional management. September 2013 November 2014 Faculty of Agricolture, Portici, Via Università 100 University, research field Forming as the assignee of a scholarship as part of a P.O.N. training project act to train experts in the genetic improvement of vegetable plants; internship act the understanding of the main NGS technologies (in particular platform ILLUMINA) and bioinformatics analysis. Page 1 - Curriculum vitae of

2 Understanding of the main techniques for genetic improvement of vegetable plants through lectures, workshops and training. Understanding of the procedures for the preparation of library for DNA-Seq, RNA-Seq and mirna-seq. Dates (from to) March 2013 September 2014 Wind, Infostrada Fixed and mobile telephony Outbound operator Proposed contracts for SIM business and business fixed for Business client and closure appointments with sales agents. Dates (from to) December 2012 March 2013 University of Naples Federico II, complex of Monte Sant'Angelo, Department of Structural Biology and Functional University, research Employed with a collaboration contract Coordinator of the PhD: "The optimal nutrition: the theoretical - practical" Dates (from to) Febbraio 2012 Novembre 2012 University of Naples Federico II, complex of Monte Sant'Angelo, Department of Structural Biology and Functional University, research field Volunteer training Heterologous expression, purification and physico - chemical characterization of human RNase 5 / 7. Chemical modifications of human RNase 5 borne by the cysteine residues (bromopropilammina, 2,4-vinylpyridine and cystammina) and study of the effect of such changes on the activity of human RNase 5 against 7 strains of P. aeruginosa Page 2 - Curriculum vitae of

3 Dates (from to) January 2010 February 2012 University of Naples Federico II, complex of Monte Sant'Angelo, Department of Structural Biology and Functional University, research Internship Master thesis entitled "Analysis of bactericidal activity human RNase 5" Heterologous expression and purification of RNase 2, 5 and 7 from zebrafish; heterologous expression and purification of human RNase 5 and 7; enzymatic activity assays (zymography, precipitation assays, assay Elmann, reaction kinetics. Bactericidal activity assays and cell proliferation assays on eukaryotic cells (MTT assay) in the study on bactericidal activity of human RNase 5 in patients with Cystic Fibrosis in order to isolate CAMPS present in its primary sequence. Transformation with recombinant plasmids and bacterial cultures in liquid medium and solid; chromatography (HPLC, FPLC, by affinity chromatography MBP trap, GS trap and His trap, gel filtration); Use AKTA Prime, AKTA Purifier and HPLC Perkin - Helmer. Using centrifuges, ultracentrifuges, concentration and dialysis in Amicon, dialysis. Electrophoresis on polyacrylamide gel; agarose gel electrophoresis, PCR, digestion with restriction enzymes, ligase and extraction of nucleic acids. Dates (from to) March 2008 October 200 ENI Gas and Light and TELETU Energetic, fixed telephony, commercial field Outbound operator Proposals of trade contracts for the supply of electric power and natural gas and home phone lines. Dates (from to) January 2004 March 2007 Restaurant Il Castello de Vita Restaurants Waiter Table service Page 3 - Curriculum vitae of

4 EDUCATION AND TRAINING Dates (from to) 23, February 2012 Name and type of organization providing education and training Principal subjects/occupational skills covered University of the Study of Naples, Federico II The curriculum has allowed me to consolidate and deepen the already possessed theoretical knowledge of chemistry, biochemistry, genetics, genetic engineering, molecular biology and bioinformatics base. The internship thesis I have done (lasting 25 months) in a biochemistry lab has allowed me to gain practical skills in the main biochemical and molecular biology. The thesis conducted by me is entitled "Analysis of bactericidal human RNase 5". Title of qualification awarded Doctor in General and Applied Biology, curriculum "molecular", with vote 108/110 Level in national classification (if appropriate) Master s Degree Dates (from to) October 2008 Name and type of organization providing education and training Principal subjects/occupational skills covered University of the Study of Naples, Federico II The curriculum has allowed me to acquire theoretical and practical knowledge of chemistry, mathematics, physics, cytology and histology, anatomy, biochemistry, genetics, genetic engineering, molecular biology, bioinformatics, physiology and hygiene. The internship thesis lasting 3 months, I have done in a laboratory of plant physiology, allowed me to get close to the experimental techniques mainly employed and the issues addressed in this field of research. The thesis is conducted by me entitled "Analysis of the expression levels of glucose phosphate dehydrogenase in leaves and roots of O. sativum" Title of qualification awarded Doctor of Biology General and applied with vote 103/110 Level in national classification (if appropriate) Bachelor degree Dates (from to) July 2001 Name and type of organization providing education and training Principal subjects/occupational skills covered Title of qualification awarded Scientific high school state Grammar and literature, Italian, English and Latin, Mathematics and physics, chemistry, biology, history, geography and philosophy. Diploma of secondary school Page 4 - Curriculum vitae of

5 PERSONAL SKILLS Acquired in the course of life and career but not necessarily covered by formal certificates and diplomas. MOTHER TONGUE ITALIAN OTHER LANGUAGES INGLESE Reading skills Writing skills Verbal skills SOCIAL SKILLS Living and working with other people, in multicultural environments, in positions where communication is important and situations where teamwork is essential (for example culture and sports), etc. ORGANISATIONAL SKILLS Coordination and administration of people, projects and budgets; at work, in voluntary work (for example culture and sports) and at home, etc. TECHNICAL SKILLS With computers, specific kinds of equipment, machinery, etc. GOOD GOOD GOOD I CAN RELATE WITH PEOPLE OF ALL WALKS OF LIFE AND CULTURAL LEVEL THANKS TO A VERSATILE CHARACTER NATURALLY PREDISPOSED TO SOCIAL INTERACTION.I CAN INTERACT WITH PEOPLE OF DIFFERENT NATIONALITIES AND CULTURE THANKS TO A VISION OF THE WORLD OPEN AND FREE OF PREJUDICES AND THROUGH MULTIPLE EXPERIENCES OF TRAVELING ABROAD. I AM ABLE TO COMMUNICATE CLEARLY AND ACCURATELY BY ADOPTING LINGUISTIC REGISTERS AND ARGUMENTATIVE RESPONSIVE TO THE SPECIFIC LEVEL REQUIRED BY THE CONVERSATION. I M ABLE TO INDEPENDENTLY ORGANIZE MY WORK AND, IF NECESSARY, TO OTHERS IDENTIFYING AND DEFINING PRIORITIES, MEET DEADLINES AND OBJECTIVES SET AND TAKING ME THE DUE RESPONSIBILITY. I M ABLE TO WORK UNDER STRESS AND FATIGUE PRESERVING THE CLARITY AND COLDNESS NECESSARY TO ACCOMPLISH THE PURPOSES SET. PREPARATION OF GROWTH MEDIA AND BUFFERS; PREPARATION OF CELL CULTURES PROKARYOTIC AND ARCHAEALI; PREPARATION OF COMPETENT CELLS OF E. COLI TO TRANSFORMATION BY CHEMICAL METHOD AND BY AND ELECTROPORATION; PREPARATION OF PLASMID DNA; ELECTROPHORETIC ANALYSIS OF DNA USING AGAROSE GEL; PURIFICATION OF DNA FROM AGAROSE GEL OR ACRYLAMIDE (ELECTROELUTION); PCR; INSERTION OF GENE SEQUENCES INTO CLONING OR EXPRESSION VECTORS; EXPRESSION AND PURIFICATION OF RECOMBINANT PROTEINS; CHROMATOGRAPHY BY FPLC AND HPLC; ANALYSIS OF PROTEINS BY SDS-PAGE; WESTERN BLOTTING; EMSA; MANAGEMENT OF FUNDS AND ECONOMIC RELATIONS WITH SUPPLIERS FOR THE SUPPLY OF REAGENTS, KITS, GLASSWARE AND HOW MUCH NECESSARY TO THE RESEARCH. VEGETABLE CROPS AND IN VITRO MICROPROPAGATION. I M ABLE TO USE THE DIFFERENT APPLICATIONS OF MICROSOFT OFFICE, ESPECIALLY WORD, EXCEL, POWER POINT, PUBLISHER, OUTLOOK AND INTERNET EXPLORER TO USE DAILY ARTISTIC SKILLS Music, writing, design, etc. SINCE CHILDHOOD I HAVE BEEN INTERESTED IN MUSIC AND SINGING, LEARNING TO PLAY THE GUITAR AT FIRST AMATEUR AND THEN PROFESSIONAL. I PARTICIPATED IN MUSIC COMPETITIONS WITH EXCELLENT RESULTS AND I WORKED IN THE FIELD OF ANIMATION AND PIANO BAR FOR CEREMONIES AND PUBLIC EVENTS DRIVING LICENCE License B ADDITIONAL INFORMATION AUTHORIZE THE USE OF PERSONAL DATA PURSUANT TO LEGISLATIVE DECREE 196/03 Page 5 - Curriculum vitae of

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