TINA SHAHANI Curriculum Vitae

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1 TINA SHAHANI Curriculum Vitae Profession: Assistant Professor, Faculty of Medicine Dept. of Genetics and Molecular Medicine, Zanjan University of Medical Sciences (ZUMS) Address: Dept. of Genetics and Molecular Medicine Phone: Faculty of Medicine, Zanjan University Work: +98 (24) of Medical Sciences Home: +98 (24) Shahrak-e Karmandan, 13 th St. Fax: +98 (241) Zanjan, Iran Postal Code: or RESEARCH INTERESTS Investigation of endothelial cell biology in health and disease is my field of interest. With a background in cell and molecular biology, I am trying to understand molecular mechanisms of endothelial-related complications in human, particularly when coagulation Factor VIII is involved. FVIII is a key player in blood coagulation. High FVIII levels are associated with an increased incidence of venous thrombosis and thromboembolism. Conversely, reduced levels of FVIII are responsible for the most severe hereditary bleeding disorder, Hemophilia A. My Ph.D. thesis involved studying human endothelial cells from various anatomical locations as the potential sites of FVIII production that was still plausible at that time; despite our deep knowledge about the factor structure and function. The study demonstrated endothelial cells from major organs including, heart, lung, kidney, skin and intestine are capable of constitutive and regulated FVIII production. My post-doctoral project was concentrated on the human liver as the main site of FVIII production to unravel the exact FVIII producing cell type within this organ. In this study several methods were included for deep characterization of isolated cells, such as FVIII functional assays and whole RNA profiling with RNAseq. EDUCATION Post-Graduate Certificate of Permanent Education in Human Genetics, by Belgian Society of Human Genetics (BeSHG). 02/ /2012 Ph.D. in Biomedical Sciences (Emerging Concepts in Cardiovascular Medicine), Centre for Molecular and Vascular Biology, Department of Molecular and Cellular Medicine, Faculty of Medicine, KU Leuven, Belgium. Thesis title: Regulation of Coagulation Factor VIII Production by Human Endothelial Cells M.Sc. in Medical Sciences, Centre for Transgene Technology and Gene Therapy (VRC), VIB3-KU Leuven, Belgium. Thesis title: Development of Improved Hepatocyte-Specific Vectors for Hemophilia B Gene Therapy (Magna cum Laude). 1

2 B.Sc. in Cell and Molecular Biology, Azad University of Tonekabon, Tonekabon, Iran (16.6 out of 20). Post-Ph.D.: PROFESSIONAL EXPERIENCES 03/ Present Assistant Professor, Dept. of Genetics and Mol Medicine, ZUMS 02/ Present Academic Advisor and Counselor, Dept. of Genetics and Mol Medicine, ZUMS 06/2014 Comps Committee, Dept. of Genetics and Mol Medicine, ZUMS Organized and administered the Ph.D. Comprehensive Examination for 2 classes of students (a total of 11). 04/ /2012 Post-Doctoral at the Centre for Molecular and Vascular Biology, Department of Cardiovascular Sciences, Faculty of Medicine, KU Leuven, Belgium. Teaching: 03/ Present Instructor, Various topics for M.S., Ph.D., MD, and MD-PhD has been giving at ZUMS in a variety of subjects including*: Mol Basis of Human Diseases Molecular Mechanisms of Vascular Diseases Genetic Engineering Epigenome and Epigenetics Pharmacogenetic Concepts Gene Transfer Technology and Gene Therapy Methods in Molecular Biology Microscopy and Cellular Biology Methods GLP and Principles of Working in a Research Lab * Full-color pdf copies of the PowerPoint files of the lectures could be provided if requested Assistant for professor of Genetics laboratory at Tonekabon University, Iran Internships: Nov. March 2010 Feb. July 2008 From Research to Market (IP, Patenting, BP etc), K U Leuven Collaborating with Colleagues and Managing a Project, K U Leuven 2006 Researcher in Genetics Center, Imam hospital, Tehran University, Tehran, Iran 2

3 Others: Specialist in the National Project of Compiling Biotechnology and Genetics Encyclopedia, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran 2003 Chairperson of the Second National Student Congress of Biology and Biotechnology. Tonekabon, Iran , May Committee executive member at From bed to bench Congress in Gastroenterology and Liver Diseases, Research Center of Gastroenterology and Liver Diseases, Shahid Beheshti University, Tehran, Iran Dec., 2002 Articles: PUBLICATIONS Shahani T., Covens K., Lavend'homme R., Jazouli N., Sokal E., Peerlinck K., Jacquemin M. (2014) Human liver sinusoidal endothelial cells but not hepatocytes contain coagulation FVIII. Journal of Thrombosis and Haemostasis; 12(1): Shahani T., Lavend'homme R., Luttun A., Saint-Remy JM., Peerlinck K., Jacquemin M. (2010) Activation of human endothelial cells from specific vascular beds induces the release of a FVIII storage pool. Blood; 115(23): Others: Jafarpur M., Shahani T., (2000) Molecular Biology equivalent to 1 credit for B.Sc. students of Cell & Molecular Biology and Microbiology. Tonekabon University, Iran Noorzad GH., Shahani T., (2001) Complementary Genetics (Focus on Bacterial Genetics) equivalent to 2 credits. Tonekabon University, Iran Abstracts: ABSTRACTS AND SEMINARS Shahani T., Covens K., Lavend'homme R., Jazouli N., Sokal E., Peerlinck K., Jacquemin M. Human liver sinusoidal endothelial cells but not hepatocytes contain coagulation FVIII. XXIV congress of the international Society on Thrombosis and Haemostasis, The Netherlands, June 29 - July 4, 2013 (poster presentation) Shahani T., Emmerechts J., Deckx S., Hoylaerts M.F., Jacquemin M. (2011) Exposure to carbon nanotubes induces upregulation of FVIII production by human pulmonary microvascular endothelial Cells. XXIII congress of the international Society on Thrombosis and Haemostasis, Kyoto, Japan, July 2011 (poster presentation) 3

4 Shahani T., Lavend'homme R., Luttun A., Saint-Remy JM., Peerlinck K., Jacquemin M. (2010) Endogenous FVIII production by human endothelial cells from specific vascular beds. Belgian Society on Thrombosis and Hemostasis, Annual meeting, Ghent, Belgium, November 2010 (poster presentation) Shahani T., Emmerechts J., Deckx S., Hoylaerts M.F., Jacquemin M. (2010) Exposure to carbon nanotubes induces upregulation of FVIII production by human pulmonary microvascular endothelial Cells. Belgian Society on Thrombosis and Hemostasis, Annual meeting, Ghent, Belgium, November 2010 (poster presentation) Shahani T., Lavend'homme R., Luttun A., Saint-Remy JM., Peerlinck K., Jacquemin M. (2010) Extrahepatic FVIII production sites: human endothelial cells from specific vascular beds. Gordon Research Conference (Endothelial cells phenotypes in health and disease), Biddeford-Maine, USA, August 2010 (Hot topic oral presentation) Seminars: Shahani T., Jacquemin M. (April, 2013). Regulation of Coagulation FVIII production by Human Endothelial Cells. (Invited speaker) School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran. Shahani T., Jacquemin M. (Jan. 2013). Hepatic and Extra-hepatic Production of Human FVIII by Endothelial Cells. (Invited speaker) Dept. of Human Genetics, School of Medicine, Ahwaz University of Medical Sciences, Ahwaz, Iran. Shahani T., Jacquemin M. (Dec. 2012). Extrahepatic Endothelial Cells and Coagulation FVIII synthesis (Invited speaker) Stem Cell Technology Research Center, Tehran, Iran. Shahani T., Jacquemin M. (Apr., 2012). Regulation of Coagulation FVIII Production by Human Endothelial Cells. (Invited Speaker) Sanquin, The Netherlands. VARIOUS COMPETENCIES Languages: Persian (native), English (fluent), Arabic and French (basic) Laboratory Skills: Molecular Biology and DNA recombinant technology: DNA and RNA extraction & purification, Cloning, PCR, RT-PCR, Q-PCR (Primer/probe and SYBR Green), gel electrophoresis, microbial culture Microscopy: Widefield, Confocal and Standard fluorescence microscopy 4

5 Protein Production and Purification: mammalian cell transfection, protein purification by chromatography (laboratory scale) Human Primary Cell Isolation and Culture Working with Laboratory Animals (mice) Immunofluorescent staining, Immunoassay, Chromogenics assays, flow cytometry RNAseq analysis (Next generation sequencing for gene expression profiling in medical and human studies) Others: Establishment of Biotechnology association at Tonekabon University, Iran Establishment of student branch of Iranian Society for Biotechnology, Tehran, Iran Member of the University Biotechnology Publishing Center, Tonekabon University, Iran Adviser of Intelligence center of National Youth Organization, Iran REFERENCES Marc Jacquemin, M.D., Ph.D. Associate Professor at the Centre for Molecular and Vascular Biology, Department of Cardiovascular Sciences, Faculty of Medicine, KU Leuven: Life Sciences Research Partners v.z.w. O&N I Herestraat 49 - box 913, 3000 Leuven Jean-Marie Saint-Remy, M.D., Ph.D. Full Professor at the Centre for Molecular and Vascular Biology, Department of Cardiovascular Sciences, Faculty of Medicine, KU Leuven: O&N I Herestraat 49 - box 911, 3000 Leuven Mohammad Hossein Sanati, Ph.D. Associate professor, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. (Ph.D. Thesis Promoter) Work: Fax: Marc.Jacquemin@med.kuleuven.be (Primary Investigator and the former head of my Ph.D. lab) Work: Fax: jean-marie.saintremy@med.kuleuven.be (My employer in 2002) Work: mhsanati@yahoo.com 5

6 Rozenn Quarck, Ph.D. Assistant Professor, Pneumology Department, Faculty of Medicine, KU Leuven: O&N I Herestraat 49 - box Leuven Christel Van Geet, M.D., Ph.D. Full Professor, Molecular and Vascular Biology UZ Herestraat 49 - box Leuven (Thesis Commitee) Work: Fax: Rozenn.quarck@med.kuleuven.be (Thesis Commitee) Work: or Fax: christel.vangeet@uzleuven.be 6