BIOTECHNOLOGY SCIENCE CENTRION FERMENTATION SYSTEM INNOVATIVE BIOTECHNOLOGY SCIENCE COMPANY. Copyright 2016 CENTRION Science. All rights reserved.

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1 BIOTECHNOLOGY SCIENCE CENTRION FERMENTATION SYSTEM INNOVATIVE BIOTECHNOLOGY SCIENCE COMPANY Copyright 2016 CENTRION Science. All rights reserved.

2 CENTRION Company Overview All about Centrion Fermentation and innovative research Cultivation solutions System. CENTRION is product brand has played its honorable role of serving customer with wide range of differently-customized equipments. We to provide for our customers needs whether they are customized products, technical support or other wise In light of quality maintenance through quality inspection for all products we guarantee no one Will be better than us as we have competitive edges on quick delivery system & reasonable price also offer. CENTRION has its own professional research & development team of scientists and product specialists that are supported by a sales team. We focus on offering cultivation solution to the life sciences market and has worked continuously to satisfy the strict facility standards required for Biotechnology. Thank you. INNOVATIVE BIOTECHNOLOGY SCIENCE COMPANY 01

3 CENTRION Certificate ISO9001 ISO14001 INNOVATIVE BIOTECHNOLOGY SCIENCE COMPANY 02

4 CENTRION Business Areas INNOVATIVE BIOTECHNOLOGY SCIENCE COMPANY 03

5 CENTRION Technology Mechatronics P&ID Vessel Mechanical Seal Agitator Control Machining Motor control Sensor monitoring Control by Touch screen CENTRION Technology Laser Welding Pipe welding Lathe Milling Bending Fermentor Bioreactor Rotor dynamic Process Simulation Bioprocess Stability GMP Line Design Down Stream Magnetic Rotor Balacing Vibration Motor INNOVATIVE BIOTECHNOLOGY SCIENCE COMPANY 04

6 CENTRION Technology Design Analysis Processing Fatigue analysis and evaluation of various experimental techniques Enclosures. Thermal fluid dynamics simulation technology. Precision 2D, 3D Modeling design, manufacturing processing technology. Second high-speed rotating body, a pressure vessel mechanism design and development techniques. INNOVATIVE BIOTECHNOLOGY SCIENCE COMPANY 05

7 CENTRION Technology Small culture Mass culture Concentrate recovered refine Final Product Lab-scale Bioreactor Pilot or Plant Bioreactor Centrifuge Ultra Filtration Chromatography Column & devices cgmp Facility Establish - Bioprocess Simulation INNOVATIVE BIOTECHNOLOGY SCIENCE COMPANY 06

8 CENTRION Producing Ability Japanese B.E Marubishi Co.,Ltd exports products production. (By applying the ISO9001, ISO14001 quality system and quality products manufactured in safe and clean production facilities). INNOVATIVE BIOTECHNOLOGY SCIENCE COMPANY 07

9 Cooperation Partners Japan's B.E Marubishi Co.,Ltd with a suitable fermentor with international standards and quality standards in technology partnership We continue to export to the completion of product development. 08

10 CENTRION After Service Plan 1 CUSTOMER Request for problem solution by or telephone 2 CENTRION 3 CENTRION Receiving problems and firstly, to send temporary solution by on-line If first solution is not worked properly, centrion technical staff visit user s site in order to repair 4 CUSTOMER To send corrective action report to prevent same problems 2 nd solution Requets for solution CUSTOMER Receiving problems CENTRION Cause analysis CENTRION 1 st solution) INNOVATIVE BIOTECHNOLOGY SCIENCE COMPANY 09

11 CENTRION Quality Inspection System Introducing electronic measuring quality inspection system for improving quality of products and to minimize the failure rate conducting an inspection. 1 DO 2 ph 3 Temp 0 to 200% of the concentration of DO outputs the measuring inspection ph 0~14 of the concentration of ph outputs the measuring inspection Temperature of 0~150 outputs PT100 sensor measuring inspection 4 Analog Input Output 4 ~ 20[mA], 0 ~ 1[V], 0 ~ 5[V], 0 ~ 10[V] The type sensor input output method selection measuring inspection 5 RS232C RS232C Communication port measuring inspection INNOVATIVE BIOTECHNOLOGY SCIENCE COMPANY 10

12 BIOTECHNOLOGY SCIENCE BENCHTOP CONVENIENCE FERMENTATION SYSTEMS BIOCANVAS LF In Touch With Life Science INNOVATIVE BIOTECHNOLOGY SCIENCE COMPANY Copyright 2016 CENTRION Science. All rights reserved. 11

13 All about CENTRION fermentation and cultivation innovative research solutions system. Desingned for easy handling Culture experiments applications Simple Operation (Operation Made Simple) Complete Packages & User-friendly interface Exellent Value Feature 1. Easy operation by finger, before experimentation of fermentation easy to calibrate ph, DO, temperature, foam Sensors in use of User interface main control screen 2. Distinctive functions include various culture programming operations to control 3. Products designed for different culture experiment and applications, operation method touch screen controller and the easy-way compact design takes up only a small space, even a small laboratory space can be used efficiently. 4. CENTRION Fermentation system is able to connect to any PC for real-time recording and control within the vessel and Intelligent self diagnostic system to maintain fermentation process. 5. Easy control of external device by fermentation controller (O₂/CO₂analyzer, gasmixer, balancer, Level, external pump, analog printer) 6. Eight ways microprocessor controller system using parallel communication software data Logging, remote control, including integrated control. 7. Agitation, Temp, ph, DO, ORP, O2, Antifoam, MFC, Feed Pump, GasMix and additional parameters such as analog signals and can be controlled through a variety. 8. 4Gas signals available to send control signals to directly 4gas controller from main control system 9. Feed function is linked to the DO Cascade and the culture of a variety of conditions that can be applied to the linked control program. 10. Data exporting interface USB port, One PC can monitor and manage all of data by network even each controller. (1ch~6ch) ml ~ 15L Total Vol. Vessel is available in a wide range. 12. Vessels can be easily disassembled and made the operation and the sensor and pump, analyzer, such as in / output is easy. 13. Single Jacket / Double Jacket and can be used to select the drive motor. 12

14 Standard products Display Agitation Range 7inch Wide Touch TFT LCD 10 ~ 1500rpm Basic Motor drive Time Range Max. Noise Level Environment AC Servo Motor, BLDC Motor AC Induction Motor 100hours 59Minutes 59Seconds <55dB(A) Ambient Temp 0~50, Humidity 85%RH Sensor TEMP ph DO Anti Foam ORP Optional Power Requirements Fuse protection Weight AC110~220V, 50/60Hz,Single Phase, 500W (FreeVoltage) 10A 10Kg OD O2 CO2 MFM or MFC Pressure Balancer Control System Built-in SCADA System Feed Control Mode Communication port Ethernet Record Output USB * Built-in SCADA System - Voltage Specifications : 90~260V 50/60Hz Free Voltage - Built-in type SMPS Module UL certification. - CE product certification to enable component selection and PCB ARTWORK. - PWM frequency control AC Servo Motor, Ac induction Motor, BLDC Motor and Is, slow down as fast and smooth implementation. - RS232 x 2, RS422 or RS485 communication port, USB port, apply - Analog Input: 12 points, analog output: 12 points, analog record: 12 points - Temp. PH, DO Cascade,ORP,OD,O2,Co2,Agitation,MFC,Pressure Balancer Control Fed-Batch Culture by DO, ph Interlock pump Control 4 x Built-in Feeding Pump(Boxer or Watson-Malow) External pump 2ea use 1PC 1-6 connected to one controller (after completion of standard controller progress) Data logging, trend graph PC Contorl: process control (PID, the upper and lower values, programs, cascade, Feed) Each sensor can be output by selecting data. D-SUB 25Pin Female Type, USB Excel file stored separately. Measured data, setup data stored in the USB Peristaltic pump Pump Motor type Speed range Resolution Control mode 4built-in pumps, two external pump (optional) AC Motor or DC Motor, minimum speed is 1rpm 0~70rpm 1rpm Programmable PID Feeding control Pump can be assigned for Acid, Base, Antifoam, Feed Bottom Heating Plate Range Up to 90 Resolution 0.1 Power source V~50/60Hz or V~50/60Hz With electrical safety cutoff switch Ordering information Cat. NO. FSBC-LF-L01 Description LAB Fermentor Controller 13

15 Specifications Vessel Total Volume (Liter) Working Volume (Liter) Material Etc. 1.5L 3L 5L 7L 10L 15L Borosilicate glass Autoclavable, SUS316L Stainless steel for Topplate and all fittings Single & Double 1.5L~15L Total Vol. ph, DO, Foam,Level,Pressure Probe, Addtion Ports, Exhaust Port, Baffles 316L,Condenser Aeration Flow rate 0~10LPM 0~20LPM Option Sparger Inlet Filter Air Flow Meter / Mass Flow Controller / Mass Flow Manual Standard : Ring Sparger / Micro Sparger 0.2 μm Disposable Hydrophobic Filter Agitation Drive Direct Top Drive Servo Motor 200W~400W,BLDC Motor / Single Mechanical seal Temperature Range Impellers Thermostat system 10~1500rpm Rushton Standard With Fermentation / Pitched Blade Standard With Cell Culture Marine Blade or Spin Filter Note : Customized impellers are available 0~150 ±0.1 / pt100ω Probe Heating&Cooling PID Control / Built-in Heat Exchanger / Automatic Cooling Water Valve ph Range / Resolution 0~14pH / 0.01pH Probe Control mode Electrode Autoclavable, InPro 3030-MettlerToledo (temperature range - 0 ~ 140'C, the maximum pressure - 6bar) Programmable PID Control system DO Range / Resolution 0~200% or 0~20ppm / 0.1% Probe Control mode Electrode Autoclavable Polarographic or Galvanic Oxygen Sensor -Mettler Toledo Programmable PID Control system DO Cascade to Agitation, Mas Flow, Feeding Pump Control - Oxygen enrichment module (optional) - Gas Mixing Station module (optional) ORP Range / Resolution Measuring range ~ mv. / 1mV Probe Electrode Autoclavable Redox potential, Oxidation of measurements-mettler Toledo (Temperature range - 0 ~ 140'C, the maximum pressure - 2.5bar) Anti Foam Range / Sensor Conductivity 0 ~ 300kΩ (Measuring the amount of foam) OD (Optional) Range / Sensor Measuring range EBC EBC FTU (Turbidity measurement) MFM or MFC (Optional) Range / Sensor 0~10L/min (Air flow measurement) / Mass Flow Meter / Mass Flow Control Level Sensor (Optional) Control Electrode type Hi/Low Level control Balancer (Optional) Control 9,999.99g / RS232C Measured by the amount of weight on the scale output Analog Input & output Control Various sensors are available by utilizing the analog input / output port Analog Recording Control It can be the sensor output data value via the analog recording 14

16 Main Control Screen (Graphical User Interface) User-oriented access display interface functions Main screen / sub screen, enter the touch of a button can TREND Culturing the elapsed time value in accordance with a graph representation of the respective sensor To select and display a graph in which the sensor can be selected The Trend graph screen makes it simple to track and export data on up to Twelve process If you need to update the controller Connect the USB to the front panel USB Port Update. PC SCADA program can be remotely controlled in auto manual mode 15

17 Configuration Layout Temp,pH,DO, Foam,ORP Sensor Port Analog Recoder RS232 Port Analog Port LAN Port AC out Power in Servo Motor Air Port in 7 Touch LCD Vessel Agitator Cold&Hot finger Feed Pump Air Flow Meter Power S/W USB/RS232C Port 16

18 Multi Controller Network System Connect the LAN Cable can control the controllers 1CH~6CH. Internet Modem(LAN) PC Program One PC can monitor and manage all of data by Wi-Fi network even each controller. LAN I-PAD Smart Phone Note Book 17

19 Lighting Module Items Lighting LAB Photo Bioreactor Lighting, the essential component for the photosynthesis reaction. We now offer special designed lighting module for your photosynthesis reaction. Light power and spectral composition,temperature and aeration gas composition can be set control. LED illumination with full spectrum imitated natural sunlight as closely as possible by emitting light peaks at 430nm and 630nm, without NDIR and ultraviolet spectrum components. Applied to microalgae, marine bio-industry, use a clean fuel production, Photosynthetic Microorganism culture. Specification Light intensity control range Light module Light Color Max. Lux Control Mode Power Voltage % Selectable - Fluorescent lamp or LED White, RED, Blue Max lux On/off or set of the lighting Controller 110V-220V, 50/60Hz, 10A Features Adjustable light intensity Fluorescent light source Expendable up to 3 lighting modules Scales-up Ideal for Pilot Custom Fabrication Easy operation and Long life Ordering information Cat. NO. FSBC-LF-PB01 Description Photo Bioreactor Autoclavable Fermentor 3L 15L White Red Blue 18

20 Ordering Information Single Vessel Cat. NO. Total Vol. (Working Vol.) Inner Dia. (mm) D Inner Height (mm) H Electrode Length (mm) H:D FSBC-LF L(1L) Ø :1 FSBC-LF L(2.5L) Ø :1 FSBC-LF L(3L) Ø :1 FSBC-LF L(5L) Ø :1 FSBC-LF L(7L) Ø :1 FSBC-LF L(12L) Ø :1 Durable stainless steel supporting rods and bottom plate are designed. Single glass type : Pyrex, Stainless316L. Fast cooling/heating is achieved through the inner cooling coil and heating base unit. Double Vessel Cat. NO. Total Vol. (Working Vol.) Inner Dia. (mm) D Inner Height (mm) H Electrode Length (mm) H:D FSBC-LF L(2.5L) Ø :1 FSBC-LF L(3L) Ø :1 FSBC-LF L(5L) Ø :1 FSBC-LF L(7L) Ø :1 FSBC-LF L(12L) Ø :1 Double glass jacketed type vessel is spcially designed for temperature sensitive Circulating water in double jacket for effective temperature control by large contact surface. Connected to extra Water Bath for temperature control. Provides sophisticated temperature control. 19

21 Ordering Information Bowl Vessel Cat. NO. Total Vol. (Working Vol.) Inner Dia. (mm) D Inner Height (mm) H Electrode Length (mm) H:D FSBC-LF L(5L) Ø :1 FSBC-LF L(7L) Ø :1 FSBC-LF L(12L) Ø :1 Stainless Double Jacket in upper body, Single Glass in under body Circulating water in double jacket for effective temperature control by large contact surface Connected to extra Water Bath for temperature Single Round Vessel Cat. NO. Total Vol. (Working Vol.) Inner Dia. (mm) D Inner Height (mm) H Electrode Length (mm) FSBC-LF L(1L) Ø :1 H:D FSBC-LF L(2.5L) Ø :1 FSBC-LF L(3L) Ø :1 FSBC-LF L(5L) Ø :1 FSBC-LF L(7L) Ø :1 Vessle with an inner coil to achieve a fast cooling. Single round vessel: round type under body. Temperature control: Glass surrounding heating blanket. Usually applied in animal cell fermentation. PBR Vessel (Photo type) Cat. NO. Total Vol. (Working Vol.) Inner Dia. (mm) D Inner Height (mm) H Electrode Length (mm) H:D FSBC-LF L(2.5L) Ø :1 FSBC-LF L(3L) Ø :1 FSBC-LF L(5L) Ø :1 FSBC-LF L(7L) Ø :1 FSBC-LF L(10L) Ø :1 Single vessel type, LED Bar inside to make growth by exposing light into algae Illumination : 430nm, 680nm, White,Red,Blue LED LED inside / flourscent jacket 20

22 Ordering Information Part Name Cat. NO. Description Rushton 6- Blade Impeller FSBC-LF-IM01-01 FSBC-LF-IM01-02 Rushton 6-Blade Impeller Ø50, 1.5L iter Vessel, 3ea/pk Rushton 6-Blade Impeller Ø60, 3L iter Vessel, 3ea/pk FSBC-LF-IM01-03 Rushton 6-Blade Impeller Ø72, 5~7L iter Vessel, 3ea/pk FSBC-LF-IM01-04 Rushton 6-Blade Impeller Ø80 10~15L iter Vessel, 3ea/pk Rushton turbine impeller is located from middle to bottom and basically used to agitate liquid in vessel. It makes the flow to the same direction with turning radius and has features of paddle and propeller types to be available for high-speed rotation by minimizing resistance. General model to be used due to the highest ventilation efficiency Available for the agitator assemblies, both lipseal. Part Name Cat. NO. Description Pitched Blade Impeller FSBC-LF-IM02-01 Pitched Blade Impeller Ø60, 3Liter Vessel, 2ea/pk FSBC-LF-IM02-02 Pitched Blade Impeller Ø72, 5~7Liter Vessel, 2ea/pk FSBC-LF-IM02-03 Pitched Blade Impeller Ø80 10~15Liter Vessel, 2ea/pk More efficient than flat blade impeller Flow is discharged both axially and radially depending on the angle Part Name Cat. NO. Description Pitched Paddle Impeller FSBC-LF-IM03-01 Pitched Paddle Impeller Ø65, 3Liter Vessel, 2ea/pk FSBC-LF-IM03-02 Pitched Paddle Impeller Ø85, 5~7Liter Vessel, 2ea/pk FSBC-LF-IM03-03 Pitched Paddle Impeller Ø100 10~15Liter Vessel, 2ea/pk More efficient than flat blade impeller Flow is discharged both axially and radially depending on the angle 21

23 Ordering Information Part Name Cat. NO. Description Marine Impeller FSBC-LF-IM04-01 Marine Impeller Ø60, 3Liter Vessel, 1ea/pk FSBC-LF-IM04-02 Marine Impeller Ø80, 5~7Liter Vessel, 1ea/pk FSBC-LF-IM04-03 Marine Impeller Ø100 10~15Liter Vessel, 1ea/pk Applied to damageable cell It stirs liquid from down to up rotating in low-speed since it has the large surface of wings Part Name Cat. NO. Description Hollowed Paddle Impeller FSBC-LF-IM05-01 FSBC-LF-IM05-02 Hollowed Paddle Impeller Ø60, 3Liter Vessel, 1ea/pk Hollowed Paddle Marine Impeller Ø80, 5~7Liter Vessel, 1ea/pk FSBC-LF-IM05-03 Hollowed Paddle Marine Impeller Ø100 10~15Liter Vessel, 1ea/pk It stirs liquid from up to down rotating in low-speed since it has the large surface of wings. Extra additional it Part Name Cat. NO. Description Foam Breaker FSBC-LF-IM06-01 Foam Breaker 70, 3Liter Vessel, 1ea/pk FSBC-LF-IM06-02 Foam Breaker 90, 5~7Liter Vessel, 1ea/pk FSBC-LF-IM06-03 Foam Breaker ~15Liter Vessel, 1ea/pk Our customized foam breaker helps you to maintain the proper foam level. Installed in upper side of inside shaft, used in bursting fermentation foam. It is not used in the case of animal cell fermentation since there is barely any foam. 22

24 BIOTECHNOLOGY SCIENCE BIOCANVAS SF IN-SITU STERILIZABLE FERMENTATION SYSTEMS In Touch With Life Science INNOVATIVE BIOTECHNOLOGY SCIENCE COMPANY Copyright 2016 Centrion Science. All rights reserved. 23

25 Pilot Production Scale Fermentation System Pilot SIP(Sterilization-in-place) Fermentor The CENTRION brand offers pilot and profuction scale bioreactor/fermentation systems for all of your large scale fermentation needs. BIOCANVAS SF Fermentor designs order-made equipment that is designed to best fit the culture conditions, culturing the object you want. stainless steel vessel and in situ Automatic sterilization System and how to use a separate sterile devices do not need to be used, in large fermentation scale-up is possible equipment. BIOCANVAS SF of sterile fermentation system (in situ automatic sterilization system) is designed to be easy to use as a lab scale fermentor. Heater has been installed on the jacket of the vessel can raise the temperature to a level to sterilize the vessel, as well as temperature control of the vessel does not require a separate sterilization system. Culturing cells and microbes could not be any easier with our full automation control of sterilization and fermentation process. BIOCANVAS SF Fermentor can be used to develop new products in the company's laboratory or university. Features Intelligent 17" wide touch screen Industrial PC fermentation controller PLC Controller is applied for minimizing size and making simple set-up process CPU and sensor board into One-board solve problems immediately High density fermentation : DO-stat, ph-stat, Exponential feeding, etc DO Cascade function and automation control Remote control by PC software Data Logging system Automatic head lift system built-in to move Top plate easily Everything on one skid frame : Easy to install and move additional vessels and vessel controllers Accurate temperature control with cooler and heater Automatic flow control: mass flow controller All-in-one system including seed fermentor and main fermentor Applied stainless pipe transfer line for perfect sterilization and user s convenience Top Plate approach adopted in the European Design The level of cgmp IQ / OQ compliance Head Lifting System Head plate is lifted by air cylinder and guided by the two sets of linear motion guide Safety pin is used to hold the lifted head plate Lifting system is made of stainless steel(sus 304) that it is rustles Basic system with the 50L-500L capacity 24

26 Pilot Production Scale Fermentation System Pilot Fermentor Pilot scale bioreactor standard specifications Vessel volume Agitation Total Capacity(L) 30L 50L 70L 100L 150L 300L 500L 1000L Working Volume(L) 21L 35L 50L 70L 100L 210L 350L 700L Drive & Range Control 30L ~500L : AC motor Top drive(mechanical seal driving) 50~1,000 ±1 RPM(In the condition of no load) Manual or automatic control of agitation speed Impellers Rushton turbine impeller, Foam breaker(sus 316L) Sensor & Control Agitation Type Magnetic hall sensor, PID control Top Drive(Mechanical Seal) or Bottom Drive(Magnetic Coupling) Head Lift The head lift system is powered by air cylinder and guided by two sets of linear motion guide Materials & Finish Vessel/Piping SUS 316L, SUS 304(electrolytic polishing) Sterilization Heat exchanger or inject steam into jacket.(105 ~130 ) Aeration Sparger Ring sparger(sus 316L) Filter Inlet(0.2 μm ), Outlet filter(0.2 μm ) ph Range / Electrode 2.0~12.0 ph of set point, PID control / Gel Type DO Range / Electrode 0~200% or 0~20ppm, PID control / Polarographic Type Temperature Range & Sensor Ambient + 3 ~80 (0.1 ), RTD(Pt-100), PID control Pressure Control The back pressure is controlled manually and/or automatically Control Panel 17 Touch Screen Color LCD Control program Process, Trend, Calibration Mode control display. all the programmed setting values will be automatically stored into the built-in memory. Data Storage Processor Setting Communications PLC system Automated sterilization process Automated fermentation program Valve control Memory stick, printer, computer, etc with the RS-232C communication protocol ph, DO, Pressure, Temperature and Agitation speed According to customer requirements and the intended use by changing some standards it can be produced. The control software also can be produced according to customer requirements. 25

27 Pilot Production Scale Fermentation System In-situ Sterilizable Fermentor BIOCANVAS SF Series Pilot scale bioreactor standard specifications Vessel Ratio (H/D) - 2:1 - Total Vol. L / Working Vol. L Vessel Pressure Certification Stamp - No Stamp / ASME Stamp Vessel Surface finish - Inside / outside : 0.8 μm / 1.2 μm Sight glass 1 ea : Oblong type Top Plate - Exhaust/Port/Pressure Gauge with diaphragm /Pressure Transmitter/Foam Sensor/Septum Port 4ea/ Inoculum Port Upper Port - Aeration/Aeration by pass/circle Light/ Rupture Disk Lower Port - Sampling / ph / DO / Temp / Etc. Harvest Vessel Temp, Pressure Inner Vessel design pressure : -1~ 4 bar(g) Inner Vessel working pressure : 2 bar(g) Inner Vessel Material: SUS 316 L Inner Vessel Design temp : 150 C Inner Vessel Working temp : 10 ~ 65 C Inner Vessel SIP temp : 121 C Jacket design pressure : Max. 6 bar(g) Jacket working pressure : Max. 4bar(g) Jacket Material : SUS 304 According to customer requirements and the intended use by changing some standards it can be produced. The control software also can be produced according to customer requirements. 26

28 Pilot Production Scale Fermentation System BIOCANVAS SF Series (30L~1000L) Aeration Air Filter Housing Head Lift Agitator Control Valve Main Controller Vessel Sight Glass Peristaltic Pump Sampling Port Heat. Exchanger Reagent Bottle Sensor Port Harvest Frame Ordering information Cat. NO. Description Cat. NO. Description FSBC-SF-S01 In-situ Sterilizable Fermentor 30L FSBC-SF-S05 In-situ Sterilizable Fermentor 150L FSBC-SF-S02 In-situ Sterilizable Fermentor 50L FSBC-SF-S06 In-situ Sterilizable Fermentor 300L FSBC-SF-S03 In-situ Sterilizable Fermentor 70L FSBC-SF-S07 In-situ Sterilizable Fermentor 500L FSBC-SF-S04 In-situ Sterilizable Fermentor 100L FSBC-SF-S08 In-situ Sterilizable Fermentor 1000L According to customer requirements and the intended use by changing some standards it can be produced. The control software also can be produced according to customer requirements. 27

29 Pilot Production Scale Fermentation System In-situ Sterilizable Fermentor BIOCANVAS SF Series Pilot scale bioreactor standard specifications Main Control System Specification * LG PLC CONTROLLER. Control Cabinet : Body case with touch screen display 17 Resistive touch color LCD panel for, data input and display/touch Screen Industrial PC unit On/Off diaphragm valves with air solenoid valves Automation program & software Functions: ph, DO, Foam, Temperature, Agitation, Peristaltic pump control,calibration & Recalibration, Etc. Real time data as a graphs for rpm, temp, DO, ph, and foam Existing data will be graphed with its own real-time system Displays and saves date, time, rpm, temperature, DO, ph, and foam Data logging software for PC: Spread sheet format & Graph type of parameters View Excel: Accumulated data in Excel format Fed Batch - ph stat, DO stat, user feeding profile, and exponential feeding On/Off & Auto Mode setting Automatic DO control - Feed pump Alarm Function - All measured and control parameters: alarm low/high limits - Screen display Automatic/Manual sterilization system Operation mode: Stop/Sterilization/ Cooling/Culture Set cooling temp User setting and programmable / Security: password protected SCADA functionality can be added(optional) (Remote monitoring the system operation via internet) The control software also can be produced according to customer requirements. 28

30 Pilot Production Scale Fermentation System SCADA SYSTEM * Introduction to SCADA SYSTEM Focus on remote monitoring and control system or SCADA monitoring and control system, also known as a data acquisition system for monitoring and control functions says. SCADA systems are analog or digital signals on the communication path to the remote device, the status information data to the remote small device (remote terminalunit) to collect, receive, record and display on the remote device to the central control system which monitors control. * SCADA SYSTEM control practices They are connected by a relay module local controllers PC 1 as Each local controller to the remote control. The local controller of P / V, S / V, Valve ON / OFF, Control State collects data in real time. The data collected is DB (Data Base) is stored in Graph, the report output. - The central control room at System Run / Stop, Valve On / Off, S / V, Control Command and set the remote control. User Login grading to enhance the security features. This is connected to the separate program SCADA communication protocol matched DLL files are also provided. 29

31 Lighting Module Items Lighting Pilot Photo Bioreactor PBR (Photobioreactor: photobioreactor) is The particular microorganisms that require light from microorganisms and microorganisms such as that just Chlorella growing in the sea water of the proper amount of light, temperature and carbon dioxide because the photosynthetic this microorganism as plant haejumyeon supply are self-culture well and so may reduce the carbon dioxide for photosynthesis by giving the current supplied to the carbon dioxide in accelerating the global warming on the photobioreactor effectively the advantage of producing a material by culturing a nutrient and via Chlorella. Photobioreactor will be a gas which is supplied carbon dioxide to the fermentor, unlike normal culturing marine microalgae of different culture conditions than are many different Chlorella production design the device in the structures of each micro algae culture well. Is attached to the vessel with a source of light, LED light, and light intensity depending on the culture conditions and the culture medium to adjust the lighting time, and so on. It is possible to precisely measure the growth of biomass, cleaning materials and health food, cosmetics (chlorella) and is used to cultivate a lot. - LED illumination with Full spectrum imitates natural sunlight as closely as possible by emitting light peaks at 430nm and 630nm, without NDIR and ultraviolet spectrum - Remote` control by PC software Data Logging system components Specification Light intensity control range Light module Light Color Max. Lux Control Mode Power Voltage % Fluorescent LED Lamp White, RED, Blue Max lux On/off or set of the lighting Controller 110V-220V, 50/60Hz, 15A Features Adjustable light intensity Fluorescent light source Expendable up to 3 lighting modules Scales-up Ideal for Pilot Custom Fabrication Easy operation and Long life Ordering information Cat. NO. FSBC-PP-PB01 Description Photo Bioreactor Autoclavable Fermentor 30L 200L 30

32 Lighting Module Items Lighting Pilot Photo Bioreactor Temp. Range & sensor Sparger & Gas mode Inlet Filter Ambient 3 ~80, RTD(Pt-100), PID control Micro sparger, 2 gas and 4 gas mixer(option) 0.2 μm air filter cartridge Filter & Condenser 0.2 μm air filter cartridge, SUS 316 ph Range ph Electrode DO Range Heating System Light sourse Device LED Lighting Light Regime Peristaltic pump Control Recording 0~14.0 ph of set point, PID control Gel Type 0~20ppm or 0~100%, PID control Cartidge heater Selectable - Fluorescent lamp or LED Intensity adjustable from 0 to 100 %, Maximum light intensity1000 µmol(photon)/m 2/ s Static or dynamic (sinus, daylight, pulse form) 4 constant speed pumps each assignable to a wide variety of function for control of acid, base, foam a nd feed ph, DO, Temp., Foam, etc. Pilot PBR Fluorescent LED lamp Case 31

33 BIOCANVAS Customers want and diverse culture will be drawn correctly. HEAD OFFICE Bucheon Techno Park Ssangyong 3cha 397, Seokcheon-ro, Ojeong-gu, Bucheon-si, Gyeonggi-do, Rep, of KOREA Tel : +82_32_624_2060 Fax : +82_32_624_ / sales@centrion.co.kr Copyright 2016 Centrion Science. All rights reserved. V2.0