OIE Reference Laboratory Reports Activities

Transcription

1 OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : :02:35 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Infectious myonecrosis Address of laboratory: Aquaculture Pathology Laboratory School of Animal & Comparative Biomedical Sciences University of Arizona Tucson, AZ UNITED STATES OF AMERICA Tel.: Fax: address: fengjyu@ .arizona.edu Website: Name (including Title) of Head of Laboratory (Responsible Official): Kathy Tang-Nelson, Associate Research Professor (Interim Director) Name (including Title and Position) of OIE Reference Expert: Kathy Tang-Nelson, Associate Research Professor Which of the following defines your laboratory? Check all that apply: Academic OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally none not applicable 0 0 Direct diagnostic tests Nationally Internationally conventional and real-time RT-PCR yes Histology yes ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2016

3 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients infected tissues RT-PCR produced approx 1-3 g approx 1-3 g 1 Africa Americas Asia and Pacific Europe Middle East Tissue sections Histology produced approx 20 slides 2 Africa Americas Asia and Pacific Europe Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, standardise and validate, according to OIE Standards, new for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? OIE Reference Laboratory Reports Activities,

4 Name of OIE Member Country seeking assistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses BELGIUM varied 8 0 BELIZE varied 18 0 CANADA December 1 0 ECUADOR varied 14 0 INDIA varied 76 0 INDONESIA varied 40 0 NEW CALEDONIA varied 48 0 RUSSIA February 1 0 SINGAPORE June 2 0 THAILAND varied 90 0 THE NETHERLANDS varied 9 0 TUNISIA June 1 0 UNITED KINGDOM varied 5 0 KOREA (REP. OF) April 1 0 VENEZUELA December Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? 4 OIE Reference Laboratory Reports Activities, 2016

5 Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided INDONESIA Shrimp diseases diagnostic workshop in conjunction with an OIE-Twinning Project on-site (Indonesia) visits, s, visiting our laboratory in the US. COSTA RICA s KOREA (REP. OF) NORWAY SPAIN THAILAND Postdoctoral training MEXICO SINGAPORE PERU SAUDI ARABIA provide consultation on biosecurity protocols. Dr. Fernando Aranguren from our laboratory visited the shrimp and fish industry and assess the biosecurity status. ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? OIE Reference Laboratory Reports Activities,

6 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country OIE-Twinning Program 3 years To improve the candidate laboratory compliance with OIE diagnostic standard for crustacean disease/shrimp disease Brackishwater Aquaculture Development Center (BADC)-Situbondo INDONESIA ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 6 1). Aranguren LF, Han JE, Tang KFJ (in press) Enterocytozoon hepatopenaei (EHP) is a risk factor for acute hepatopancreatic necrosis disease (AHPND) and septic hepatopancreatic necrosis (SHPN) in the Pacific white shrimp Penaeus vannamei. Aquaculture. 2) Han JE, Tang KFJ, Pantoja CP, Piamsomboom P. (2017) Evaluation of a reliable non-invasive molecular test for the diagnosis of the causative agent of acute hepatopancreatic necrosis disease (AHPND) of shrimp. Aquaculture Report 5: )Han JE, Tang KFJ, Piamsomboom P, Aranguren LF (2017). Characterization and pathogenicity of acute hepatopancreatic necrosis disease natural mutants, pirabvp (-) V. parahaemolyticus, and pirabvp (+) V. campbellii strains. Aquaculture 470: )Tang KFJ, Han JE, Aranguren LF, ble-white B, Schmidt MM, Piamsomboon P, Risdiana E, Hanggono B (2016) Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp. J Invertebrate Pathology 140: ) Han JE, Tang KFJ, Pantoja CR, Lightner DV, Redman RM, Groumellec ML (2016) Detection of a new microsporidium Perezia sp. in shrimps Penaeus monodon and P. indicus by histopathology, in situ hybridization and PCR. Dis. Aquatic Org. 120: ) Jun JW, Han JE, Tang KFJ, Lightner DV, Kim J, Seo SW, Cheng C, Giri SS, Yun S, Kim HJ, Kim SG (2016) Abundant potential of bacteriophage pvp-1 application: agent combating Vibrio parahaemolyticus related to acute hepatopancreatic necrosis disease (AHPND) in shrimp. Aquaculture 457: b) International conferences: 3 1) Detection of a microsporidian parasite (Perezia sp.) in penaeid shrimp from Madagascar, Mozambique, and the Kingdom of Saudi Arabia through histological, in situ hybridization, and PCR analyses. (2016) Kathy FJ. Tang*, Jee Eun Han, Donald V. Lightner. World Aquaculture Society meeting, Sarabaya, Indonesia. 2) Detection of white spot syndrome virus (WSSV) in commercial shrimp feeds by PCR (2016) Bambang Hanggono*,Yani Lestari N, Fatmawati, Sumini, Jee Eun Han, Kathy FJ. Tang. World Aquaculture Society meeting. Sarabaya, Indonesia. 3) Enfermedades Importantes en el Cultivo de Camarón en Latinoamérica con Énfasis en NHP, WSSV y AHPND y 6 OIE Reference Laboratory Reports Activities, 2016

7 Signos Clínicos, Muestro y Técnicas de Diagnóstico (2016) Aranguren F. Peru Sanipes. c) National conferences: 2 1) RECENT STUDIES ON ACUTE HEPATOPANCREATIC NECROSIS DISEASE (AHPND, ALSO KNOWN AS EARLY MORTALITY SYNDROME, EMS) (2016) Jee Eun Han*, Kathy FJ Tang, Donald V. Lightner. World Aquaculture Society meeting. Las Vegas. 2)RECENT STUDIES ON SHRIMP MICROSPORIDIAN INFECTION:ENTEROCYTOZOON HEPATOPENAEI (EHP) AND COTTON SHRIMP DISEASE (2016) Jee Eun Han*, Kathy FJ Tang, Donald V. Lightner. World Aquaculture Society meeting. Las Vegas. d) Other: (Provide website address or link to appropriate information) 0 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 2 b) Seminars: 2 c) Hands-on training courses: 2 d) Internships (>1 month): 1 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a Indonesia 20 a Saudi Arabia 20 b Peru approx 200 c Korea, rway, Spain, Mexico, Ecuador, Peru 20 b Indonesia approx 100 d Thailand 1 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? OIE Reference Laboratory Reports Activities,

8 Quality management system adopted ISO 17025:2005 Certificate scan (PDF, JPG, PNG format) UAZAquaPathLabCertScope-ANAB-V001.pdf 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited IMNV-RT-PCR and histology Accreditation body ANSI-ASQ National Accreditation Board 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? 8 OIE Reference Laboratory Reports Activities, 2016

9 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries Proficiency test-feb Africa Americas Asia and Pacific Europe Middle East Proficiency test-aug Africa Americas Asia and Pacific Europe Middle East ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: OIE Reference Laboratory Reports Activities,