Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Colchester Hospitals NHS Foundation Trust operating as North East Essex & Suffolk Pathology Services (NEESPS) Biochemistry Department Contact: Stephen Gee Ipswich Hospital Tel: +44 (0) Heath Road Ipswich Website: Suffolk IP4 5PD Testing performed at the above address only HUMAN BODY FLUIDS DETAIL OF ACCREDITATION General Biochemistry diagnosis. Quantitation of: Albumin Roche c702 ADO188 (Blood) Using bromcresol green Urine Albumin/microalbumin ADO236 using Polyclonal antihuman albumin antibodies (sheep) Blood Alcohol (Ethanol) ADO205 using alcohol dehydrogenase Alkaline Phosphatase ADO189 using p-nitrophenyl phosphate Alpha 1 Antitrypsin Ado187 using - Anti-human α1- antitrypsin antibody (rabbit): ALT ADO190 using L-alanine and 2- oxoglutarate Ammonia ADO191 using - + α-ketoglutarate + NADH Assessment Manager: VB Page 1 of 12

2 General Biochemistry (cont d ) diagnosis. Quantitation of: Anti Thyroid peroxidase antibodies ADO 161 Roche c702 Blood, Urine Amylase ADO232 (Blood) ADO241 (Urine) Using 4,6-ethylidene-(G7) p- nitrophenyl-(g1)-α,dmaltoheptaoside (ethylidene- G7PNP) Blood Angiotensin Converting enzyme ADO234 using synthetic substrate (FAPGG) Gentamycin Vancomycin ADO206 using KIMS ADO230 using - Vancomycin labelled with the enzyme glucose-6- phosphate dehydrogenase (G6PDH) AST ADO192 using - L-aspartate and 2- oxoglutarate Bicarbonate ADO194 using - phosphoenolpyruvate (PEP) Bile acids ADO276 using Thio - Nicotinamide Adenine Dinucleotide Bilirubin (Conjugated) Bilirubin (Total) Caeruloplasmin ADO195 using - diazotized sulfanilic acid ADO196 using diazonium ion ADO200 using - Anti-ceruloplasmin T antiserum (rabbit Assessment Manager: VB Page 2 of 12

3 Roche c702 Blood, Urine Calcium ADO231 (Blood) ADO244 (Urine) Using - 5-nitro-5 -methyl-bapta (NM-BAPTA) Carbamazepine ADO199 using - Anticarbamazepine antibody (mouse monoclonal) Cholesterol ADO201 using - phenol and 4- aminophenazone Non-calculated test CK (Creatinine Kinase) Complement C3 Complement C4 C-Reactive Protein Creatinine ADO202 using ADP ADO197 using Anti-human C3 antibody (goat): ADO198 using Anti-human C4 antibody (goat) ADO204 using - Latex particles coated with anti-crp (mouse ADO203 (Blood) ADO250 (Urine) Using 4-aminophenazone and HTIB a CSF Glucose ADO262 using Glucose-6- phosphate dehydrogenase oxidizes CSF Protein ADO221 using copper in alkaline solution Assessment Manager: VB Page 3 of 12

4 Roche c702 Gamma Glutamyl Transferase ADO207 using - γ-glutamyl-3- carboxy-4-nitroanilide + glycylglycine Blood, Urine Glucose ADO208 (Blood) ADO261 (Urine) ADO261 (Pleura/ascitic fluid & dialysis fluid) Using Glucose-6-phosphate dehydrogenase oxidizes HDL IgA IgG IgM Iron Lactate LD (Lactate Dehydrogenase) ADO209 using PEG-cholesterol esterase ADO210 using Anti-human IgA antibody (goat) ADO211 using Anti-human IgG antibody (goat) ADO212 using Anti-human IgM antibody (goat) ADO213 using - - Transferrin-Fecomplex ADO214 using enzyme lactate oxidase (LOD) & Peroxidase (POD) ADO215 (Blood) ADO277 (Pleura/Ascitic fluid & Dialysis fluid) Using pyruvate Blood, Urine Magnesium ADO217 (Blood) ADO270 (Urine) Using aminomethane & Xylidyl blue Assessment Manager: VB Page 4 of 12

5 Paracetamol Phenytoin Roche c702 ADO218 using aryl acylamidase & o-cresol + ammoniacal copper sulphate ADO219 uisng KIMS Blood, Urine Phosphate ADO220 (Blood) ADO271 (Urine) Using ammonium molybdate Urine 24hr Protein ADO235 using Benzethonium chloride Salicylate Theophylline ADO223 using NADH & salicylate hydroxylase ADO224 using KIMS Blood Total Protein ADO225 (Blood) using copper in alkaline solution Transferrin Triglyceride ADO226 USING Anti-human transferrin antibodies (rabbit) ADO227 using - 4-aminophenazone + 4-chlorophenol 4-(p-benzoquinone-monoimino)- phenazone Urine, Blood Urate ADO274 (Urine) ADO229 (Blood) Using Uricase & 4-aminophenazone Assessment Manager: VB Page 5 of 12

6 Roche Cobas e602 Blood, Urine Urea ADO228 (Blood) ADO275 (Urine) using - urease and glutamate dehydrogenase B12 CA125 CA153 CA19-9 CEA Cortisol ADO162 Competitive immunoassay ADO163 Sandwich immunoassay (mouse antibody) ADO164 Sandwich immunoassay (mouse antibody) ADO165 Sandwich immunoassay (mouse antibody) ADO166 Sandwich immunoassay (mouse antibody) ADO167 Competitive immunoassay (polyclonal anti-cortisol antibody (ovine)) Digoxin ADO 169 Competitive immunoassay (monoclonal mouse antibody). Ferritin ADO170 Sandwich Immunoassay. Monoclonal mouse antibody Assessment Manager: VB Page 6 of 12

7 Folate FSH HcG PEG Precipitation pre-treatment for interfering antibodies to monoclonal proteins LH Macroprolactin Oestradiol Procalcitonin Progesterone Roche Cobas e602 ADO171 Competitive immunoassay ADO172 Sandwich Immunoassay. Mouse antibody ADO176 Sandwich immunoassay (monoclonal anti-hcg antibody, mouse) LP0003 ADO177 Sandwich Immunoassay. Monoclonal Mouse antibody. LP % polyethylene glycol (PEG) solution precipitation ADO178 Competitive immunoassay. Polyclonal Rabbit antibody. ADO285 Sandwich Immunoassay. Monoclonal anti PCT Antibodies (Mouse) ADO179 Competitive immunoassay. Monoclonal Mouse antibody. Assessment Manager: VB Page 7 of 12

8 Prolactin PSA (Total) PTH SHBG T3 (Free) T4 (Free) Testosterone Roche Cobas e602 ADO180 Sandwich immunoassay. Monoclonal Mouse antibody ADO181 Sandwich immunoassay. Monoclonal mouse antibody. ADO182 Sandwich immunoassay. Monoclonal mouse antibody. Roche Cobas ISE ADO159 Sandwich immunoassay. Monoclonal mouse antibody. ADO173 Competitive Immunoassay. Monoclonal Sheep antibody. ADO174 Competitive immunoassay. Polyclonal sheep antibody. ADO183 Competitive immunoassay. Monoclonal sheep antibody. Troponin T ADO184 Sandwich immunoassay (mouse antibody) Assessment Manager: VB Page 8 of 12

9 Schedule of Accreditation TSH ADO185 Sandwich immunoassay. Monoclonal mouse antibody. Blood, Urine Chloride ADO245 (Blood) ADO247 (Urine) Ion-Selective Electrode (ISE) Potassium ADO259 Ion-Selective Electrode (ISE) Blood, Urine Sodium ADO260 (Blood) ADO273 (Urine) Ion-Selective Electrode (ISE) Blood & Pleural fluid Whole blood Faeces Blood Blood gas analysis: p.h p.co2 TCO2 (Bicarbonate) p.o2 Ionized Calcium Sodium Potassium Chloride Lactate Carboxyhaemoglobin Vitamin D IGF1 Calcoprotein Insulin Radiometer ABL 825 Flex LP0047 Diasorin Liaison XL LP0060 Assessment Manager: VB Page 9 of 12

10 Blood (Unless otherwise specified) Protein electrophoresis assays: SP 60 Helena SAS Protein electrophoresis Gel, CZE, and CZE Immunodisplacement Using SOP LP088, LP089, LP090, LP091, LP092, LP099 Cryoglobulin Paraprotein Quantitation Protein electrophoresis Paraprotein quantitation Polyclonal increase in gamma globulins Decrease in gamma region if present Protein immunoelectrophoresis/immunofixa tion Paraprotein classification IgA kappa or lambda IgM kappa or lambda IgG kappa or lambda Urine Electrophoresis Paraprotein quantification Immunofixation Lambda or Kappa free light chains Blood HbA1c Bio-Rad D100 LP042 Lithium Roche 9180 Electrolyte analyser LP009 Blood, urine Osmolality Advanced instruments Osmometer model 3320 LP0016 Assessment Manager: VB Page 10 of 12

11 CSF Xanthochromia In house procedure and Thermo electron corporation UV 500 Spectrophotometry LPO016 Sweat Sweat Chloride Sherwood scientific Model 926S chloride analyser LPO048 Renal calculi Renal calculi Brucker Alpha FITR infra-red spectrophotometer with ATR attachment LPO086 Serum Blood and Urine Light Free chains Kappa and Lambda and calculated ratio Adjusted Calcium; CADJ Albumin/ Creatinine Ratio; MAUC Aldosterone/ Renin Ratio; ALDR Anion Gap; AG Creatinine clearance; CRC Estimated Glomerular Filtration Rate (enzymatic); GFRE Globulins; GLOB LDL Cholesterol (calculated); LDL Non-HDL cholesterol; NHDL Free Androgen Index; FAI Free Light Chain Ratio; KLR Binding site Optilite SOP LP0095 Calculated tests using SOP LP0110 Assessment Manager: VB Page 11 of 12

12 Blood and Urine Protein/ Creatinine Ratio; TPC Total Cholesterol / HDL Cholesterol Ratio; CHHR Saturated Transferrin; SATR Unconjugated Bilirubin; BITC Urine Amylase 24h; AMYD Urine Calcium 24h; CAD Urine Chloride 24h; CLD Urine Creatinine 24h; CRD Urine Magnesium 24h; MGD Urine Paraprotein 24h; PPD Urine Phosphate 24h; PO4D Urine Potassium 24h; KD Urine Protein 24h; TPD Urine Sodium 24h; NAD Urine Urate 24h; UAD Urine Urea 24h; URD Calculated tests using SOP LP0110 END Assessment Manager: VB Page 12 of 12