OIE Reference Laboratory Reports Activities

Transcription

1 OIE Reference Laboratory Reports Activities Activities in 2017 This report has been submitted : :26:51 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Salmonellosis Address of laboratory: German Federal Institute for Risk Assessment National Salmonella Reference Laboratory Diedersdorfer Weg 1 D Berlin GERMANY Tel.: Fax: address: istvan.szabo@bfr.bund.de Website: Name (including Title) of Head of Laboratory (Responsible Official): Dr. Istvan Szabo Name (including Title and Position) of OIE Reference Expert: Dr. Istvan Szabo Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally Direct diagnostic tests Nationally Internationally PCR no 307 Real-time PCR yes 739 MLVA no 39 Next Generatin Sequencing no 409 Serotyping yes 3872 Test Salmonella Vaccine Strains no 78 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 4. Did your laboratory produce vaccines? 2 OIE Reference Laboratory Reports Activities, 2017

3 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? OIE Reference Laboratory Reports Activities,

4 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country Ecology from Farm to Fork Of microbial drug Resistance and Transmission 5 years Provide scientific evidence and high quality data on anti-microbial resistance (AMR) in the food chain various BELGIUM BULGARIA DENMARK FRANCE ITALY POLAND SPAIN SWEDEN THE NETHERLANDS New approaches in identifying and characterizing microbiological and chemical hazards 2 years Capacity building of Next Generation Sequencing in for surveillance of foodborne pathogens (E.coli, Salmonella) various DENMARK ITALY POLAND UNITED KINGDOM ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 3 Borowiak, M., Hammerl, J.A., Fischer, J., Szabo, I. and Malorny, B. (2017) Complete genome sequence of Salmonella enterica subsp. enterica Serovar Paratyphi B sequence type 28 harboring mcr-1. Genome Announcement 5, pii: e Borowiak, M., Szabo, I., Baumann, B., Junker, E., Hammerl, J.A., A., K., Malorny, B. and Fischer, J. (2017) VIM-1- producing Salmonella Infantis isolated from swine and minced pork meat in Germany. Journal of Antimicrobial Chemotheraphy 72, 3. Szabo, I., Grafe, M., Kemper, N., Junker, E. and Malorny, B. (2017) Genetic basis for loss of immuno-reactive O- chain in Salmonella enterica serovar Enteritidis veterinary isolates. Veterinary Microbiology 204, b) International conferences: 3 Borowiak, M. (2017) Phylogeny of Salmonella Paratyphi B variant Java harbouring mcr-1. In 27th an Congress of Clinical Microbiology and Infectious Deseases. Vienna, Austria. Fischer, J., Guenther, S., Falgenhauer, L., Chakraborty, T., Roesler, U. and Roschanski, N. (2017) Comparative whole genome analyses of VIM-1 carbapenemase producing Escherichia coli and Salmonella enterica strains derived from two German chicken fattening farms. In 27th an Congress of Clinical Microbiology and Infectious Deseases. Vienna, Austria. 4 OIE Reference Laboratory Reports Activities, 2017

5 von Laer, A., Simon, S., Enkelmann, J., Fruth, A., Lachmann, R., Michaelis, K., Malorny, B., Prager, R. and Frank, C. (2017) Investigating one outbreak of Salmonella Kottbus and finding two, Germany 2017 In an Scientific Conference on Applied Infectious Disease Epidemiology. Waterfront Congress Centre, Stockholm, Sweden. c) National conferences: 5 Fischer, J., Borowiak, M., Baumann, B., Szabo, I. and Malorny, B. (2017) Ganzgenom-Sequenz-Analysen multiresistenter Salmonella Infantis Isolate entlang der deutschen Lebensmittelkette. In 36 Jahrestagung der DVG- Fachgruppe AVID - Bakteriologie. Bad Staffelstein, Germany. Malorny, B. (2017) Experiences from an NRL of the implementation of WGS. In The use of whole genome sequencing (WGS) in monitoring of antimicrobial resistance, EURL-AR training course Dansih Technical University (DTU). Fischer, J. (2017) Molecular characterisation of VIM-1 producing Enterobacteriaceae from German livestock and food. In Scientific Meeting on issues related to Carbapenem-resistance in animal derived isolates Bundesinstitut für Risikobewertung, Berlin-Marienfelde. Szabo, I. (2017) Vorkommen von Salmonellen in Tier, Lebens- und Futtermitteln in Deutschland: Aktueller Bericht aus dem NRL Salmonella. In Fortbildungsveranstaltung für den Öffentlichen Gesundheitsdienst Bundesinstitut für Risikobewertung, Berlin-Marienfelde. Malorny, B. (2017) Gesamtgenomsequenzierung in der Lebensmittelsicherheit - Von der Theorie bis zur Anwendung. In 9 Marienfelder Gepräch, Arbeitstreffen von BfR und BVL mit den Leitungen der Landesuntersuchungsämter. Bundesinstitut für Risikobewertung. d) Other: (Provide website address or link to appropriate information) 1 Information system on consumer protection and food safety: FIS-VL Fachinformationssystem für Verbraucherschutz und Lebensmittelsicherheit ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? OIE Reference Laboratory Reports Activities,

6 Quality management system adopted ISO Certificate scan (PDF, JPG, PNG format) Akkreditierungsurkunde-vom pdf 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited Serotyping of Salmonella spp. Detection of Salmonella spp. (ISO 6579) Detection of Salmonella spp. with PCR and real-time PCR Identification of Salmonella Enteritidis with real-time PCR Conformation of d-tartrat fermentation in Salmonella spp. with PCR Identification of S. Enteritidis Vaccine Strains with real-time PCR Accreditation body Identification of mono- and bipasich S. Typhimurium with real-time PCR MLST of Salmonella isolatesp. PFGE of Salmonella isolatesp. MLVA of Salmonella isolatesp. 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? 6 OIE Reference Laboratory Reports Activities, 2017

7 ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 OIE Reference Laboratory Reports Activities,

8 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries Participant EURL ring trial : Salmonella detection in food 33 Participant EURL ring trial : Salmonella detection in primary production 23 Participant EURL ring trial : Salmonella detection in primary production 36 Participant EURL ring trial : Salmonella PFGE typing 36 Participant EURL ring trial : Salmonella serotyping in food approx. 18 Organiser NRL ring trial: Salmonella detection in poultry faeces in phrame of the National Salmonella Control Program 49 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: 8 OIE Reference Laboratory Reports Activities, 2017