FunctionMAX TiterEnhancer Overview. Customer name here Account Manager name here Date

Transcription

1 FunctionMAX TiterEnhancer Overview Customer name here Account Manager name here Date 1 3/8/2017

2 Have you ever experienced a fed-batch process that made you question if titers were maximized as much as possible? you wanted to rework with minimal changes just to improve cost of goods? presented a critical challenge: raise titers or stop the project? 2

3 Current options to improve fed-batch outcomes Approach Concerns What to Avoid New Class Solution Extreme ph Employee Safety & Bioreactor Control Using Extreme ph to Solubilize Ingredients Highly Concentrated, ph Neutral Additives Adding more feed Volume Constraints on Total Volume Large Volume Additions Adding Key Ingredients without Large Volume Additions Adding Exotic Ingredients Regulatory Justification & Product Quality Undefined Ingredients not found in Base Media Chemically Defined, Animal Origin-Free, Protein Free, Additive Containing Base Media Ingredients 3

4 Introducing Gibco FunctionMAX TiterEnhancer a revolutionary functional additive for CHO fed-batch processes What is it? Your New Class Solution Additive: Highly concentrated ph neutral protein-free chemically defined animal origin-free additive What It Does Improves cell productivity Provides up to double titers Requires minimal process changes Delivering higher concentrations of key ingredients than previously possible 4

5 7x Concentration Key Ingredients vs. Base Media Benefits of highly concentrated, ph neutral additive Easier delivery into culture No impact on bioreactor ph when added Avoids extreme ph hazard for technicians Reduced risk of changes in product quality 5

6 More than 2x Titer Levels Customer Case Study in 5L Bioreactor No change in growth Increase in q P More than double titer levels without Significant increase in viable cell density 6

7 Addition of FunctionMAX TiterEnhancer means Process Advantage Potential Impact Just add to current process No extensive rework Partial replacement of feed possible Avoid modifications to bioreactor working volume to see benefit Only small addition (10%) needed to quickly see benefit Reduced process development time to achieve target titers No exotic ingredients Lower regulatory burden Only delivers ingredients cells utilize in base medium Less explanation to regulators required 7

8 Potential Economic Benefits More protein per run Offers reduced cost per gram of protein produced More capacity made available for other uses or Fewer complete reactor cycles (prep, run, clean) Fewer runs Fewer purification cycles Simple prep & addition with feeds Enables reduced prep times Minimizes failure risk & related costs 8

9 FunctionMAX TiterEnhancer Key Benefits Up to double titers Boosts plateau in specific productivity Delivers key ingredients needed late in culture Deliver higher concentrations at neutral ph, enabling Easier delivery into culture No impact on bioreactor ph Avoidance of extreme ph hazards for technicians Reduced risk of changes in product quality Require minimal, if any, process optimization Can be added to current process or as partial feed replacement Avoids adjustments to bioreactor working volume May reduce process development time Contributes to lower regulatory compliance risk vs. alternatives 9

10 FunctionMAX TiterEnhancer is Available Liquid or AGT dry format Product Quantity Cat. No Gibco FunctionMAX TiterEnhancer liquid 100 ml bottle A Gibco FunctionMAX TiterEnhancer liquid 500 ml bottle A Gibco FunctionMAX TiterEnhancer AGT additive 10 L (1500g AGT) A Gibco FunctionMAX TiterEnhancer AGT additive 100L (15000g AGT) A Related Products Gibco CD CHO medium Gibco CD OptiCHO Medium Gibco CHO CD EfficientFeed A & B feeding supplements 1L bottle 10 L (243 g AGT) 1L bottle 10 L (193 g AGT) A 1L bottle B 1L bottle A 10L AGT Kit B 10L (541.5 g AGT) A A A A A For Research or Manufacturing Use Not intended for any animal or human therapeutic or diagnostic use 2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation and/or its affiliate(s) or their respective owners. 10

11 Visit the FunctionMAX TM Website 11