Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 HR, UK Laboratory locations: Unit 7A4 Victoria Road Avonmouth Bristol BS11 9DB Contact: Mr A Barrett-Jones Tel: +44 (0) ax: +44 (0) info@onescientific.co.uk Website: s covered by the organisation and their relevant activities details Activity code Address Unit 7A4 Victoria Road Avonmouth Bristol BS11 9DB Local contact Mr A Barrett-Jones Testing Microbiological Address Unit Avon Riverside Estate Victoria Rd Bristol BS11 9DB Local contact Mr A Barrett-Jones Administration Sample Receipt and Registration Testing Chemical Assessment Manager: CP Page 1 of 10

2 2 Pine Trees, Cher t s ey L an e, Stai nes - u po n - T ham es, TW 18 HR, UK DETAIL O ACCREDITATION OOD AND OOD PRODUCTS Chemical Testing Acidity (ph) CN02 using Hannah ph meter Water activity CN01 using AquaLab 4TE OOD, OOD PRODUCTS AND ENVIRONMENTAL SWABS Allergen Testing Detection and quantification of: Almond CA10 RIDASCREEN AST Almond Kit (R-Biopharm Rhone Ltd, R6901) Beta-lactoglobulin CA06 RIDASCREEN AST β- Lactoglobulin Kit (R-Biopharm Rhone Ltd, R4902) Casein Crustacea Egg Gluten CA05 RIDASCREEN AST Casein Kit (R-Biopharm Rhone Ltd, R4612) CA16 RIDASCREEN AST Crustacean Kit (R-Biopharm Rhone Ltd, R712) CA08 RIDASCREEN AST Egg Kit (R-Biopharm Rhone Ltd, R6402) 1) CA02 RIDASCREEN Gliadin Competitive Kit (R-Biopharm Rhone Ltd, R7001) 2) CA04 RIDASCREEN Gliadin Competitive Kit (R-Biopharm Rhone Ltd, R7021) Assessment Manager: CP Page 2 of 10

3 2 Pine Trees, Cher t s ey L an e, Stai nes - u po n - T ham es, TW 18 HR, UK OOD, OOD PRODUCTS AND ENVIRONMENTAL SWABS (Cont d) Allergen Testing (cont d) Detection and quantification of: Histamine CA14 RIDASCREEN Histamine Kit (R-Biopharm Rhone Ltd, R1604) Mustard CA12 RIDASCREEN AST Mustard Kit (R-Biopharm Rhone Ltd, R6152) Peanut CA09 RIDASCREEN AST Peanut Kit (R-Biopharm Rhone Ltd, R6202) Sesame CA11 RIDASCREEN AST Sesame Kit (R-Biopharm Rhone Ltd, R7202) Soya CA07 RIDASCREEN AST Mustard Kit (R-Biopharm Rhone Ltd, R7102) OOD, OOD PRODUCTS AND ENVIRONMENTAL SWABS Molecular Testing Qualitative detection of: Almond CA27 Sureood PREP Advance (Protocol 1) Kit (R-Biopharm Rhone Ltd, S105) with Sureood ALLERGEN ID Almond Kit (R- Biopharm Rhone Ltd, S104) and AriaMx RT-PCR Assessment Manager: CP Page of 10

4 2 Pine Trees, Cher t s ey L an e, Stai nes - u po n - T ham es, TW 18 HR, UK OOD, OOD PRODUCTS AND ENVIRONMENTAL SWABS (Cont d) Molecular Testing (cont d) Qualitative detection of: Celery CA15 Sureood PREP Advance (Protocol 1) Kit (R-Biopharm Rhone Ltd, S105) with Sureood ALLERGEN ID Celery Kit (R- Biopharm Rhone Ltd, S105) and AriaMx RT-PCR ish CA1 Sureood PREP Advance (Protocol 1) Kit (R-Biopharm Rhone Ltd, S105) with Sureood ALLERGEN ID ish Kit (R- Biopharm Rhone Ltd, S110) and AriaMx RT-PCR Molluscs CA17 Sureood PREP Advance (Protocol 1) Kit (R-Biopharm Rhone Ltd, S105) with Sureood ALLERGEN ID Molluscs Kit (R- Biopharm Rhone Ltd, S11) and AriaMx RT-PCR Peanut CA26 Sureood PREP Advance (Protocol 1) Kit (R-Biopharm Rhone Ltd, S105) with Sureood ALLERGEN ID Peanut Kit (R- Biopharm Rhone Ltd, S10) and AriaMx RT-PCR Beef CA18 Sureood PREP Basic Kit (R-Biopharm Rhone Ltd, S1052) with Sureood ANIMAL ID Beef IAAC Kit (R-Biopharm Rhone Ltd, S611) and AriaMx RT-PCR Goat CA2 using Mericon Animal Identification Goat Kit and AriaMx RT-PCR Assessment Manager: CP Page 4 of 10

5 2 Pine Trees, Cher t s ey L an e, Stai nes - u po n - T ham es, TW 18 HR, UK OOD, OOD PRODUCTS AND ENVIRONMENTAL SWABS (Cont d) Molecular Testing (cont d) Qualitative detection of: Horse CA21 Sureood PREP Basic Kit (R-Biopharm Rhone Ltd, S1052) with Sureood ANIMAL ID Horse IAAC Kit (R-Biopharm Rhone Ltd, S6118) and AriaMx RT-PCR Pork CA19 Sureood PREP Basic Kit (R-Biopharm Rhone Ltd, S1052) with Sureood ANIMAL ID Pork IAAC Kit (R-Biopharm Rhone Ltd, S6114) and AriaMx RT-PCR Poultry CA20 Sureood PREP Basic Kit (R-Biopharm Rhone Ltd, S1052) with Sureood ANIMAL ID Chicken IAAC Kit (R-Biopharm Rhone Ltd, S6115) and AriaMx RT-PCR Sheep CA22 using Mericon Animal Identification Sheep Kit and AriaMx RT-PCR Turkey CA24 Sureood PREP Basic Kit (R-Biopharm Rhone Ltd, S1052) with Sureood ANIMAL ID Turkey IAAC Kit (R-Biopharm Rhone Ltd, S6116) and AriaMx RT-PCR Assessment Manager: CP Page 5 of 10

6 2 Pine Trees, Cher t s ey L an e, Stai nes - u po n - T ham es, TW 18 HR, UK ENVIRONMENTAL SAMPLES Microbiological Testing Environmental Swabs Aerobic colony count 0 C for 48 hrs M001 In-House Method using PCA pour plate incubated at 0 C for 48 hrs Aerobic colony count 0 C for 72 hrs M001a based on BS EN ISO 48-1:201 Enterobacteriaceae (presumptive) M002 based on BS EN ISO :2017 Coliforms (presumptive) M00 based on BS ISO 482:2006 Coagulase positive Staphylococcus including Staphylococcus aureus (confirmed) M005 based on BS EN ISO :1999 β-glucuronidase positive Escherichia coli 1) M02 based on BS ISO :2001 2) M04 based on BS ISO :2001 by membrane technique Yeasts and Moulds M010 based on BS ISO :2008 Pseudomonas spp. (presumptive) M004 based on BS EN ISO1720:2010 Assessment Manager: CP Page 6 of 10

7 2 Pine Trees, Cher t s ey L an e, Stai nes - u po n - T ham es, TW 18 HR, UK ENVIRONMENTAL SAMPLES (cont'd) Microbiological Testing (cont'd) Detection: Environmental Swabs (cont d) Salmonella spp (confirmed) 1) M006 based on BS EN ISO 6579:2002 +A1:2007, with confirmation using BioMérieux API 20E and Pro-Lab antisera 2) M07 using BioMérieux MINI VIDAS SLM with confirmation using BioMérieux API 20E and Pro-Lab antisera Listeria spp (including species identification) 1) M007 based on BS EN ISO :1996 +A1:2004 with confirmation/speciation using Microbact Listeria 12L 2) M072 using BioMérieux MINI VIDAS LIS with confirmtion/ speciation using Microbact Listeria 12L Exposed Settle Plates Aerobic colony count 0 C for 48 hrs M001 In-House Method using PCA plates incubated at 0 C for 48 hrs Yeasts and Moulds M010 based on BS ISO :2008 OOD AND OOD PRODUCTS Microbiological Testing Aerobic colony count 0 C for 48 hrs M001 In-House Method using PCA pour plate incubated at 0 C for 48 hrs Aerobic colony count 0 C for 72 hrs M001a based on BS EN ISO 48-1:201 Assessment Manager: CP Page 7 of 10

8 2 Pine Trees, Cher t s ey L an e, Stai nes - u po n - T ham es, TW 18 HR, UK OOD AND OOD PRODUCTS (cont d) Microbiological Testing (cont d) (cont d) Anaerobic mesophilic colony count M044 (customer specified method) Bacillus cereus (presumptive) M009 based on BS EN ISO 792:2004 Clostridium perfringens (presumptive) M008 based on BS EN ISO 797:2004 Enterobacteriaceae (presumptive) M002 based on BS EN ISO :2017 Coliforms (presumptive) M00 based on BS ISO 482:2006 β-glucuronidase positive Escherichia coli Coagulase positive Staphylococcus including Staphylococcus aureus (confirmed) Lactic acid bacteria (presumptive or confirmed) Listeria spp Pseudomonas spp. (presumptive) 1) M02 based on BS ISO :2001 2) M04 based on BS ISO :2001 by membrane technique M005 based on BS EN ISO :1999 M011 based on ISO 15214:1998 M026 based on ISO :2017 with confirmation/ speciation using Microbact Listeria 12 L M004 based on BS EN ISO1720:2010 oods with AW >0.95 Yeasts & Moulds M010 based on BS ISO :2008 Assessment Manager: CP Page 8 of 10

9 2 Pine Trees, Cher t s ey L an e, Stai nes - u po n - T ham es, TW 18 HR, UK OOD AND OOD PRODUCTS (cont d) Microbiological Testing (cont d) oods with AW 0.95 Yeasts & Moulds M051 based on BS ISO :2008 Detection: Salmonella spp (confirmed) 1) M006 based on BS EN ISO 6579:2002 +A1:2007, with confirmation using BioMérieux API 20E and Pro-Lab antisera 2) M07 using BioMérieux MINI VIDAS SLM with confirmation using BioMérieux API 20E and Pro-Lab antisera Listeria spp (including species identification) 1) M007 based on BS EN ISO :1996 +A1:2004 with confirmation/speciation using Microbact Listeria 12 L 2) M072 using BioMérieux MINI VIDAS LIS with confirmtion/ speciation using Microbact Listeria 12 L WATERS Microbiological Testing Potable, Process, Pools and Spas Total aerobic colony count at 22 C and 7 C Total Coliforms and Escherichia coli M015 based on The Microbiology of Drinking Water (2012), Part 7 M016 based on The Microbiology of Drinking Water (2016), Part 4B using MLGA Assessment Manager: CP Page 9 of 10

10 2 Pine Trees, Cher t s ey L an e, Stai nes - u po n - T ham es, TW 18 HR, UK WATERS (cont d) Microbiological Testing (cont d) Potable, Process, Pools and Spas (cont d) Enterococci (presumptive) M017 based on The Microbiology of Drinking Water (2012), Part 5 Pseudomonas aeruginosa (confirmed) M025 based on The Microbiology of Drinking Water (2015), Part 8B Sulphite-Reducing Clostridia M018 based on The Microbiology of Drinking Water (2015), Part 6A Detection and enumeration: Cooling towers, Potable, Process, Pools and Spas Legionella pneumophila sg 1 Legionella pneumophila sg 2-14 Legionella sp (not L. pneumophila sg 1-14) Presumptive Legionella sp (not L. pneumophila sg 1-14) M027 based on BS :1998/ ISO 1171:1998 with plates incubated at 7 C and confirmation using Oxoid Latex agglutination kit END Assessment Manager: CP Page 10 of 10