Prof. Steven S. Saliterman

Transcription

1 Department of Biomedical Engineering, University of Minnesota Prof. Angela Panoskaltsis-Mortari s BMEn 5361, 3D Bioprinting

2 Human scale bioprinting. 2D and 3D bioprinting Mandible bone reconstruction. In-situ printing. Segmenting chronic wounds. Workflow 2D segmentation methods and comparison. Fresh printing. Comment on tool paths. Bioprinting a bronchus.

3 A & B - Cell-laden hydrogels, 3T3 Fibroblasts, dye colored red and green (Dil and DiO). PCL - Supporting poly(εcaprolactone) (PCL) polymer. S - Sacrificial Pluronic F-127 hydrogel. Kang, H. W., S. J. Lee, I. K. Ko, C. Kengla, J. J. Yoo, and A. Atala. "A 3d Bioprinting System to Produce Human-Scale Tissue Constructs with Structural Integrity." [In English]. Nature Biotechnology 34, no. 3 (Mar 2016):

4 Kang, H. W., S. J. Lee, I. K. Ko, C. Kengla, J. J. Yoo, and A. Atala. "A 3d Bioprinting System to Produce Human-Scale Tissue Constructs with Structural Integrity." [In English]. Nature Biotechnology 34, no. 3 (Mar 2016):

5 Kang, H. W., S. J. Lee, I. K. Ko, C. Kengla, J. J. Yoo, and A. Atala. "A 3d Bioprinting System to Produce Human-Scale Tissue Constructs with Structural Integrity." [In English]. Nature Biotechnology 34, no. 3 (Mar 2016):

6 Kang, H. W., S. J. Lee, I. K. Ko, C. Kengla, J. J. Yoo, and A. Atala. "A 3d Bioprinting System to Produce Human-Scale Tissue Constructs with Structural Integrity." [In English]. Nature Biotechnology 34, no. 3 (Mar 2016):

7 Gholami, P., M. A. Ahmadi-pajouh, N. Abolftahi, G. Hamarneh, and M. Kayvanrad. "Segmentation and Measurement of Chronic Wounds for Bioprinting." [In English]. IEEE Journal of Biomedical and Health Informatics 22, no. 4 (Jul 2018):

8 Gholami, P., M. A. Ahmadi-pajouh, N. Abolftahi, G. Hamarneh, and M. Kayvanrad. "Segmentation and Measurement of Chronic Wounds for Bioprinting." [In English]. IEEE Journal of Biomedical and Health Informatics 22, no. 4 (Jul 2018):

9 Region-Based Region growing - Wound segmentation can be obtained based on the intensity similarity of wound pixels. Active Contours without Edges (Chan-Vesse Model) - this algorithm detects objects based on region similarities, not relying on gradients. Edge-Based Methods Most chronic wounds have clear and distinguished boundaries, which make it possible to perform the segmentation based on the edges in the images. Livewire (Intelligent Scissors) - a seed point is chosen, and the mouse controlled pointer follows the edges, allowing exclusion of detached skin (outlier edges). Parametric Active Contours Snakes A deformable energy minimizing curve. Texture-Based Methods Houhou-Thiran-Bresson model (HTB) Gholami, P., M. A. Ahmadi-pajouh, N. Abolftahi, G. Hamarneh, and M. Kayvanrad. "Segmentation and Measurement of Chronic Wounds for Bioprinting." [In English]. IEEE Journal of Biomedical and Health Informatics 22, no. 4 (Jul 2018):

10 Gholami, P., M. A. Ahmadi-pajouh, N. Abolftahi, G. Hamarneh, and M. Kayvanrad. "Segmentation and Measurement of Chronic Wounds for Bioprinting." [In English]. IEEE Journal of Biomedical and Health Informatics 22, no. 4 (Jul 2018):

11 Gholami, P., M. A. Ahmadi-pajouh, N. Abolftahi, G. Hamarneh, and M. Kayvanrad. "Segmentation and Measurement of Chronic Wounds for Bioprinting." [In English]. IEEE Journal of Biomedical and Health Informatics 22, no. 4 (Jul 2018):

12 A. Femur from CT. B. Printed in alginate. C. Uniaxial tensile testing. D. Coronary from MRI. E. Arterial tree in alginate. F. Arterial tree in fluorescent alginate. G. Zoom of vessel wall H. Darkfield positioning. I. Time-lapse dye flow Hinton, TJ et al. Three-dimensional printing of complex biological structures by freeform reversible embedding of suspended hydrogels. Science Advance 23 Oct (2)

13 Bioprinting rasters may be in Cartesian vs parametric* form. Consider extrusion-based (EBB) rather than droplet-base (DBB) or laser-based bioprinting (LBB) which are less common and based on manufacturer specific tool paths. Why use one method vs the other? Issues arise with resulting printed gradients as excess accumulation of bioink can occur at directional changes. Parametric* modeling/toolpath may be helpful for lumen and other hollow shape object printing. Control of porosity (e.g. bones) *Parametric implies a variable is dependent on other variables commonly used to express the coordinates of the points that make up a geometric object such as a curve or surface.

14 A. Design of a continuous toolpath and bilayer bioprinted vertebra. B. Toolpath for graded wound device and bilayer printed device. C. Comparison of toolpath using cartesian vs parametric coordinates. Ozbolat, I.T. 3D Bioprinting Fundamentals, Principles and Application. Elsevier, Amsterdam 2017.

15 A. CAD model of aorta with controlled material composition along the parametric distance u. B. Femur model with toolpath for controlled porosity along the distance u; sample double layer structure bioprinted using sodium alginate hydrogels. Ozbolat, I.T. 3D Bioprinting Fundamentals, Principles and Application. Elsevier, Amsterdam 2017.

16 Biobots, Repetier Host and Slic3R setup for BioBot1

17 Pluronic F127 is a sacrificial bioink that can be used for temporary support or to create channels, vessel or vasculature for bioprinting purposes. Liquid when cold, gel when warmed. Streamlined printable biomaterial that supports 3D printed cellular constructs. Biobots, Pluronic F127

18 Open Repetier Host and load your CAD File. Biobots, Repetier Host and Slic3R setup for BioBot1

19 Biobots, Repetier Host and Slic3R setup for BioBot1

20 Print pressure and temperature are set in BioBots software after the sliced G code file is saved and uploaded. Biobots, Repetier Host and Slic3R setup for BioBot1

21 Human scale bioprinting. 2D and 3D bioprinting Mandible bone reconstruction. In-situ printing. Segmenting chronic wounds. Workflow 2D segmentation methods and comparison. Fresh printing. Tool paths. Bioprinting a bronchus.