OIE Reference Laboratory Reports Activities

Transcription

1 OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : :15:36 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Babesiosis Address of laboratory: Istituto Zooprofilattico Sperimentale della Sicilia (IZSSi), Italian Reference Centre for Anaplasma, Babesia, Rickettsia, Theileria (C.R.A.Ba.R.T.), via Gino Marinuzzi 3, 90129, Palermo ITALY Tel.: ex Fax: address: santo.caracappa@izssicilia.it Website: Name (including Title) of Head of Laboratory (Responsible Official): Dr. Santo Caracappa DVM, Health Direcor of IZSSi, Director of National Reference Center for Anaplasma, Babesia, Rickettsia and Theileria (CRABaRT) Name (including Title and Position) of OIE Reference Expert: Santo Caracappa, DVM, Health Direcor of IZSSi, Director of National Reference Center for Anaplasma, Babesia, Rickettsia and Theileria (CRABaRT) Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally B. bovis IFI YES B. caballi IFI YES B bigemina ELISA NO Babesia canis IFI NO Babesia caballi ELISA YES 30 0 Direct diagnostic tests Nationally Internationally PCR Babesia canis (ssrna) NO PCR d Babesia caballi (BC48) NO PCR Babesia ovis (ssurna) NO PCR Babesia bovis (rap1) NO PCR Babesia bigemina (rap1) NO PCR Babesia microti (ssrrna) NO Babesia spp. blood smears YES Babesia bovis SYBR Green Real Time PCR NO Babesia bigemina SYBR Green Real Time PCRe NO Babesia bovis TaqMan Real Time PCR NO OIE Reference Laboratory Reports Activities, 2016

3 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? OIE Reference Laboratory Reports Activities,

4 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients Babesia bigemina Positive control DNA PCR produced ml 1 Africa Americas Asia and Pacific Europe Middle East Babesia bigemina Positive control DNA PCR provided ml 1 Africa Americas Asia and Pacific Europe Middle East Babesia bigemina Positive control DNA PCR provided ml 1 Africa Americas Asia and Pacific Europe Middle East Babesia bovis Positive control DNA PCR provided ml 1 Africa Americas Asia and Pacific Europe Middle East Babesia bovis Positive control DNA PCR provided ml 1 Africa Americas Asia and Pacific Europe Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? 4 OIE Reference Laboratory Reports Activities, 2016

5 ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? Name of the new test or diagnostic method or vaccine developed Description and References (Publication, website, etc.) ELISA diagnostic test for cattle babesiosis Vet Ital Jan-Mar;52(1):63-9. doi: /VetIt A promising new ELISA diagnostic test for cattle babesiosis based on Babesia bigemina Apical Membrane Antigen-1. Torina A1, Cordaro A, Blanda V, D'Agostino R, Scimeca S, Scariano ME, Sireci G, Lelli R. DOI: /VetIt fluorogenic probebased PCR assays Vet Parasitol Jun 10;162(3-4): doi: /j.vetpar Epub 2009 Mar 31. Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids. Criado-Fornelio A1, Buling A, Asenzo G, Benitez D, Florin-Christensen M, Gonzalez-Oliva A, Henriques G, Silva M, Alongi A, Agnone A, Torina A, Madruga CR.DOI: /j.vetpar ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? OIE Reference Laboratory Reports Activities,

6 Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided FRANCE Optimization of genetic assays for Anaplasma, Babesia and theileria Reference material FRANCE Recommendation for IFAT for the detection of antibodies against B. canis and B. gibsoni Reference material and technical support UNITED KINGDOM Recommendation for molecular and serological procedure for the diagnosis of VBD Reference procedures NIGERIA Advises for experimental inoculation of dogs with b. canis Consultation PERU Recommendation for serological tests for the detection of antibodies against b. bigemina and b. bovis in cattle Reference procedures ITALY Recommendation for serological tests for the detection of antibodies against b. bigemina and b. bovis in cattle Reference procedures SOUTH AFRICA Recommendation for serological and molecular for the detection of B. gibsoni Reference material and technical support RUSSIA Recommendation for serological and molecular tests for the detection of babesia Reference material ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? 6 OIE Reference Laboratory Reports Activities, 2016

7 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country Mise en place d'une plate forme et d'un réseau tuniso-italien pour la surveillance des maladies émergentes transmises par les tiques et les Culicidae (moustiques) 18 months The purpose of the project is the development of a monitoring system, based on a cross-border network for the epidemiological surveillance which will allow, through a database (containing census of the cases, the presence of carriers, prevalence, environmental data... ), to follow the evolution of zoonosis and vector borne diseases and therefore constitute an early warning system Ecole Nationale de Médecine Vétérinaire di Tunisi TUNISIA Mise en place d'une plate forme et d'un réseau tuniso-italien pour la surveillance des maladies émergentes transmises par les tiques et les Culicidae (moustiques) 18 months The purpose of the project is the development of a monitoring system, based on a cross-border network for the epidemiological surveillance which will allow, through a database (containing census of the cases, the presence of carriers, prevalence, environmental data... ), to follow the evolution of zoonosis and vector borne diseases and therefore constitute an early warning system bservatoire National des Maladies uvelles et Emergentes Mise en place d'une plate forme et d'un réseau tuniso-italien pour la surveillance des maladies émergentes transmises par les tiques et les Culicidae (moustiques) 18 months The purpose of the project is the development of a monitoring system, based on a cross-border network for the epidemiological surveillance which will allow, through a database (containing census of the cases, the presence of carriers, prevalence, environmental data... ), to follow the evolution of zoonosis and vector borne diseases and therefore constitute an early warning system Faculté des Arts et des Humanités Mise en place d'une plate forme et d'un réseau tuniso-italien pour la surveillance des maladies émergentes transmises par les tiques et les Culicidae (moustiques) 18 months The purpose of the project is the development of a monitoring system, based on a cross-border network for the epidemiological surveillance which will allow, through a database (containing census of the cases, the presence of carriers, prevalence, environmental data... ), to follow the evolution of zoonosis and vector borne diseases and therefore constitute an early warning system Institute Pasteur de Tunis TUNISIA OIE Reference Laboratory Reports Activities,

8 Vector borne pathogens 8 months Characterizing tick borne pathogens in vectors Instituto de Investigación en Recursos Cinegéticos: IREC SPAIN Vector borne pathogens in ticks from lebanon 12 months Characterizing tick borne pathogens in ticks in lebanon LEBANON ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 3 Vet Ital Jan-Mar;52(1):63-9. doi: /VetIt A promising new ELISA diagnostic test for cattle babesiosis based on Babesia bigemina Apical Membrane Antigen-1. Torina A1, Cordaro A, Blanda V, D'Agostino R, Scimeca S, Scariano ME, Sireci G, Lelli R. Ticks Tick Borne Dis Oct;7(6): doi: /j.ttbdis Epub 2016 Aug 29. Tick species, tick-borne pathogens and symbionts in an insular environment off the coast of Western France. Michelet L1, Joncour G2, Devillers E1, Torina A3, Vayssier-Taussat M1, Bonnet SI1, Moutailler S4. Persichetti MF, Solano-Gallego L, Serrano L, Altet L, Reale S, Masucci M, Pennisi MG. Detection of vector-borne pathogens in cats and their ectoparasites in southern Italy. Parasit Vectors May 10;9(1):247. doi: /s PMID: b) International conferences: 4 Mayssaa Dabaja, Valeria Blanda, Gesualdo Vesco, Rosalia D'Agostino, Salvatore Scimeca, Francesco La Russa, Santo Caracappa, Michel Afram, Alessandra Torina. Tick borne pathogens in ticks from livestock in Lebanon. Combined Conference of AITVM & STVM, September 4-8, 2016, Berlin (Germany) 13-19/02/2016 Abu Dhabi 23-25/05/2016 Paris 4/5/16 Le Scienze Omiche al servizio della diagnostica sanitaria, Palermo c) National conferences: 2 Mayssaa Dabaja, Valeria Blanda, Gesualdo Vesco, Rosalia D'Agostino, Salvatore Scimeca, Francesco La Russa, Santo Caracappa, Michel Afram, Alessandra Torina. Tick Borne Pathogens in ticks from domestic animals in Lebanon. XXIX National Congress SoIPa (Società Italiana di Parassitologia) & European Veterinary Parasitology College, June 21-24, 2016, Bari (Italy). 8 OIE Reference Laboratory Reports Activities, 2016

9 29-30/12/2016 Attività dei Centri OIE e Malattie Tranfrontaliere d) Other: (Provide website address or link to appropriate information) 0 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 8 b) Seminars: 6 c) Hands-on training courses: 0 d) Internships (>1 month): 7 OIE Reference Laboratory Reports Activities,

10 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a Spain 1 b Tunisia 2 d Germany 2 d Tunisia 1 d Iraq 2 d Bulgaria 1 d Russia 1 b Scotland 1 b USA 1 a Portugal 1 a Greece 2 a UK 2 b Jordan 1 b Greece 1 a Czech Republic 1 a France 1 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO Certificate scan (PDF, JPG, PNG format) Certificato di Accreditamento n pdf 16. Is your laboratory accredited by an international accreditation body? 10 OIE Reference Laboratory Reports Activities, 2016

11 Test for which your laboratory is accredited Babesia bigemina PCR Babesia bovis PCR Babesia caballi IFI Babesia caballi PCR Babesia microti PCR Babesia canis IFI Babesia bigemina ELISA Babesia bovis IFI Babesia spp smears Accreditation body 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? OIE Reference Laboratory Reports Activities,

12 National/ International Title of event Co-organiser Date (mm/yy) Location. Participants National LXX Convegno SISVET SISVET, IZS Sicily, Unipa, Unime 06/16 Palermo 500 International 2 nd meeting of the Steering Committee IZS Sicily 06/16 Palermo 11 National Attività dei Centri OIE e malattie transfrontaliere IZS Sicily 12/16 Palermo 100 International REEV-MED: EAEVE evalutaion of the Establishments for veterinary education on the Mediterranean basin IZS Sicily, REEV- MED 06/16 Palermo 30 International Le Scienze Omiche al servizio della diagnostica sanitaria IZS Sicily 05/16 Palermo Did your laboratory participate in scientific meetings on behalf of the OIE? Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented GFTADs Sub-Regional Conference on Camel diseases 02/16 Abu Dhabi Speaker Global Overview bacterial virus and parasites 84th General Session of the World Assembly of OIE Delegates 05/16 Paris Partecipant ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? 12 OIE Reference Laboratory Reports Activities, 2016

13 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries Control of specificity and sensitivity related to tests carried out in all the laboratories partner of the restus project 3 Africa Americas Asia and Pacific Europe Middle East Control of specificity and sensitivity related to PCR techniques 1 Africa Americas Asia and Pacific Europe Middle East ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? Kind of consultancy Location Subject (facultative) Reference laboratory Head Paris Participating at the OIE annual meeting 25. Additional comments regarding your report: OIE Reference Laboratory Reports Activities,

14 14 OIE Reference Laboratory Reports Activities, 2016