OIE Reference Laboratory Reports Activities

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1 OIE Reference Laboratory Reports Activities Activities in 2014 This report has been submitted : :49:42 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Babesiosis Address of laboratory: Istituto Zooprofilattico Sperimentale della Sicilia (IZSSi), Italian Reference Centre for Anaplasma, Babesia, Rickettsia, Theileria (C.R.A.Ba.R.T.), via Gino Marinuzzi 3, 90129, Palermo ITALY Tel.: ex Fax: address: santo.caracappa@izssicilia.it Website: Name (including Title) of Head of Laboratory (Responsible Official): Dr. Santo Caracappa, DVM, PhD Name (including Title and Position) of OIE Reference Expert: Dr. Santo Caracappa, DVM, PhD Head of the Territorial Health Depertment, Head of the CRABaRT (National Centre of Reference for Anaplasma, Babesia, Rickettsia and Theileria). Veterinary Public Health Institute of Sicily Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally Babesia bovis IFI 57 0 Babesia caballi IFI 36 0 Babesia bigemina ELISA 71 0 Babesia canis IFI 37 0 Babesia caballi ELISA Direct diagnostic tests Nationally Internationally Babesia canis (ssrrna) 13 0 Babesia caballi (BC48) Babesia microti (18S) 18 0 Babesia ovis (ssurna) 71 0 Babesia bigemina (SpeI-AvaI fragment) Babesia bovis (RapI) Blood smear 91 0 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and 2 OIE Reference Laboratory Reports Activities, 2014

3 any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? OIE Reference Laboratory Reports Activities,

4 ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Title of the study Duration Purpose of the study Partners (Institutions) Mise en place d'une plate forme et d'un réseau tuniso-italien pour la surveillance des maladies émergentes transmises par les tiques et les Culicidae (moustiques) 18 months The purpose of the project is the development of a monitoring system, based on a cross-border network for the epidemiological surveillance which will allow, through a database (containing census of the cases, the presence of carriers, prevalence, environmental data... ), to follow the evolution of zoonosis and vector borne diseases and therefore constitute an early warning system Ecole Nationale de Médecine Vétérinaire di Tunisi Mise en place d'une plate forme et d'un réseau tuniso-italien pour la surveillance des maladies émergentes transmises par les tiques et les Culicidae (moustiques) 18 months The purpose of the project is the development of a monitoring system, based on a cross-border network for the epidemiological surveillance which will allow, through a database (containing census of the cases, the presence of carriers, prevalence, environmental data... ), to follow the evolution of zoonosis and vector borne diseases and therefore constitute an early warning system Observatoire National des Maladies uvelles et Emergentes Mise en place d'une plate forme et d'un réseau tuniso-italien pour la surveillance des maladies émergentes transmises par les tiques et les Culicidae (moustiques) 18 months The purpose of the project is the development of a monitoring system, based on a cross-border network for the epidemiological surveillance which will allow, through a database (containing census of the cases, the presence of carriers, prevalence, environmental data... ), to follow the evolution of zoonosis and vector borne diseases and therefore constitute an early warning system Faculté des Arts et des Humanités Mise en place d'une plate forme et d'un réseau tuniso-italien pour la surveillance des maladies émergentes transmises par les tiques et les Culicidae (moustiques) 18 months The purpose of the project is the development of a monitoring system, based on a cross-border network for the epidemiological surveillance which will allow, through a database (containing census of the cases, the presence of carriers, prevalence, environmental data... ), to follow the evolution of zoonosis and vector borne diseases and therefore constitute an early warning system Institute Pasteur de Tunis 4 OIE Reference Laboratory Reports Activities, 2014

5 ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 2 - Torina A., Moreno-Cid J.A., Blanda V., Fernández de Mera I.G., Pérez de la Lastra, Salvatore Scimeca, Marcellocalogero Blanda, Maria Elena Scariano, Salvatore Briganò J.M., Disclafani R., Piazza A., Vicente J., Gortázar C., Caracappa S., Lelli R.C., de la Fuente J. (2014) Control of tick infestations and pathogen prevalence in cattle and sheep farms vaccinated with the recombinant Subolesin-Major Surface Protein 1a chimeric antigen. Parasites & Vectors 7: Spada E., Proverbio D., Galluzzo P., Perego R., Bagnagatti De Giorgi G., Roggero N., Caracappa S. (2014) Frequency of Piroplasms Babesia microti and Cytauxzoon felis in Stray Cats from rthern Italy BioMed Research International Volume 2014 b) International conferences: 2 - Blanda V., Lelli R.C., Currò V., Randazzo K., D Agostino R., Giudice E., Caracappa S., Torina A. (2014) A NEW TAQMAN REAL TIME PCR TARGETING THE OMPA GENE FOR RICKETTSIA CONORII IDENTIFICATION 3rd European EAVLD (European Association of Veterinary Laboratory Diagnosticians) Congress - Pisa (Italy), October 12-15, 2014 Pag International Conferences of OIE Reference Laboratories and Collaborating Centres, October 2014, Seoul (Rep. of Korea) c) National conferences: 3 - Caracappa S., Persichetti M.F., Blanda V., Marineo S., Foti M., Torina A. (2014) Prevalenza di Theileria annulata nelle diverse province siciliane tra il 2011 e il 2013 XLVI CONGRESSO S.I.B Maggio Caracappa S., Blanda V., Di Pasquale C., Torina A. (2014) Sorveglianza molecolare di infezioni da Anaplasma ovis, Babesia ovis e Theileria ovis in ovi-caprini siciliani XXI CONGRESSO NAZIONALE S.I.P.A.O.C. Società Italiana di Patologia ed Allevamento degli Ovini e dei Caprini 9/12 settembre 2014 Foggia; Pag IL RUOLO DEI CENTRI DI REFERENZA OIE NEL CONTROLLO DELLE MALATTIE TRASMESSE DA ZECCHE ED ALTRI VETTORI", Palermo Dicembre 2014 d) Other: (Provide website address or link to appropriate information) 0 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? OIE Reference Laboratory Reports Activities,

6 a) Technical visits: 2 b) Seminars: 1 c) Hands-on training courses: 1 d) Internships (>1 month): 1 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country c Jordan, Kuwait, Lebanon, Oman, Yemen 2, 2, 2, 1, 1 d Germany, Turkey 6, 1 a Spain, US 1, 1 b France, Jordan, Lebanon, Tunisia 1, 1, 1, 1 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO Certificate scan (PDF, JPG, PNG format) Certificato di accreditamento del laboratorio.pdf 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited Babesia bigemina PCR Babesia bovis PCR Babesia caballi IFI Babesia caballi PCR Babesia canis IFI Babesia bigemina ELISA Babesia bovis IFI Babesia microti PCR Accreditation body 6 OIE Reference Laboratory Reports Activities, 2014

7 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? National/ International Title of event Co-organiser Date (mm/yy) Location. Participants International Third Global Conference of OIE Reference Centres OIE 04/14 Palermo Did your laboratory participate in scientific meetings on behalf of the OIE? Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented Third Global Conference of OIE Reference Centres 10/14 Incheon (Korea none none Meeting at the Ministry of Agricolture 07/14 Beirut, Lebanon none none ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? OIE Reference Laboratory Reports Activities,

8 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: The laboratory has been designed as OIE Reference laboratory on May 2014 therefore this report relates to the period from June 1st to December 31st OIE Reference Laboratory Reports Activities, 2014