Biochromatography Bring more Zen into your life and laboratory

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1 Seminar Biochromatography Bring more Zen into your life and laboratory Method development and maintaining peptide, protein, glycan and anti-body analyses by LC or LC-MS can be very time consuming and might lead to frustrations. Phenomenex has brought out a number of solutions under the name BioZen that should help to bring more peace and calm into your laboratory. BioZen gives you high efficient columns with Titanium housing, together with our professional support for a decent price. If you want to know how to bring peace within your laboratory, then you are more than welcome to our Seminar on Wednesday 30 th of May from 11.45am to 5pm (including lunch) nearby the Technology park Ghent The latest developments in column technologies and MS software and detection are been discussed by Phenomenex and Sciex

2 Enhancing Peptide Mapping Coverage with Proper Preparation and New Versatile Selectivities Peptide mapping is an extremely common method, used for sequence confirmation, lot to lot variation, and stability studies by monitoring posttranslational modifications such as deamidation and oxidation. This seminar will briefly discuss sample preparation for peptide mapping, in addition to good HPLC/UHPLC practices when doing peptide maps. Finally, an overview of PTM s and how they affect chromatography will be discussed at length. We will also be highlighting a brand-new LC portfolio designed for the analysis and characterization of biologics. Sample preparation and clean-up options, tips and limitations across steps for denaturation, reduction, alkylation, buffer exchange, digest, and pretreatment Good practices for improving peptide mapping chromatography for both HPLC and UHPLC How to expand peptide map coverage and PTM identification through versatile LC stationary phases and particle platforms New LC portfolio and biocompatible hardware designed and tested for the analysis and characterization of biologics Europe and globally as well as presenting multiple live webinars.

3 Top Down and Middle Down Analysis of Monoclonal Antibodies with Dual LC Particle Platforms Although peptide mapping is a common method for characterization of monoclonal antibodies, other methods can also give insight on monoclonal antibody heterogeneity. This seminar will give an overview on intact mass for determination of mab mass and variants such as glycoforms. Additionally, socalled middle down approaches, such as reduction and enzymatic digestion, can also be used to identify post translational modifications, such as lysine variants and oxidations. This simple approach can be used as a platform method for characterization for mab heterogeneity. Overview of reversed phase analysis of intact biologics LC particle and temperature considerations for impurity profiling by Middle Down Analysis Good practices for middle down platform method design and antibody reduction Average DAR for cysteine conjugated ADCs using intact reversed phase stationary phase Utilization of intact mass workflow for variant identification New LC portfolio and biocompatible hardware designed and tested for the analysis and characterization of biologics Europe and globally as well as presenting multiple live webinars.

4 Biologics Characterization Made Easy Location: Close to Technologiepark Gent Date: 30 th of May Time: 11,45am 5pm SCIEX invites you to learn about recent developments in analytical technologies and advances in software solutions for biopharmaceutical characterization using Mass Spectrometry X500B QTOF System - The Simple Way to Excellent Data Biologics Multiple Attribute Monitoring Made Easy Advanced workflows on TripleTOF 6600 for biologics characterization The Instructor: Henri Nicar Henri is working for Sciex as Sales Specialist Advanced Workflows; BioPharma & Omics

5 Optimizing High Molecular Aggregate Recovery in Size Exclusion Methods for Therapeutic Proteins Aggregation can potentially increase the immunogenicity of a therapeutic protein. As such, aggregate quantitation is performed throughout the development of a therapeutic method. This seminar will give an overview of the impact of mobile phase and column hardware in aggregate recovery in size exclusion chromatography (SEC). Additionally, mixed mode SEC separations using chaotropic salts will be discussed, as well as so-called Native MS or SEC-MS for protein characterization. Overview of protein aggregation and size exclusion chromatography (SEC) Good practices for SEC method development and design Optimizing protein peak shape, resolution and recovery with smart adjustments to flow rate, mobile phase and column selection Novel biocompatible hardware for improving recovery and minimizing column priming High salt methods for mab impurity profiling New LC portfolio designed and tested for the analysis and characterization of biologics Europe and globally as well as presenting multiple live webinars.

6 Improved N-Linked Glycan Analysis and Separation Power with Novel Core-Shell Particle Chemistry Glycosylation is a post-translational modification common in protein therapeutics such as monoclonal antibodies. Because it can affect immunogenicity, efficacy, and pharmacokinetics, glycosylation must be monitored throughout the drug development pathway, from early discovery and cell line development, to quality control lot release. This seminar will focus on Hydrophilic Interaction Chromatography of fluorescently labeled glycans. Using a novel stationary phase, high mannose glycans can be separated easily from other biantennary neutral glycans. Additionally, good separation of heavily sialylated glycans can also occur with optimization of gradient. Finally, a brief overview of orthogonal methods like peptide mapping and intact mass are also discussed. Overview and importance of current methods for N-linked glycan analysis Good practices for sample preparation, labeling and HILIC chromatography A novel core-shell HILIC stationary phase that offers differentiated released glycan retention profile Orthogonal techniques for relative quantitation of glycoforms New LC portfolio and biocompatible hardware designed and tested for the analysis and characterization of biologics Europe and globally as well as presenting multiple live webinars