Food DNA-Chip. CarnoCheck high sensitive. For the highly sensitive detection of 8 animal species in food products.

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1 Food DNA-Chip CarnoCheck high sensitive For the highly sensitive detection of 8 animal species in food products

2 CarnoCheck high sensitive DNA chip for the highly sensitive and simultaneous identification of 8 animal species in food products. The horse meat scandal in 2013 highlighted the potential for the abuse of the labelling of animal meat products. False declarations of meat food products lead to the compulsory testing of the origin of food products down to the species level. The scandal highlighted the fact that food could have been falsely labelled for a number of years because the analysis conducted failed to identify a sufficient number of alternative species. Microarray technology can help to establish a wider screening panel in routine food testing and to support thus the safety and integrity of the food supply chain. CarnoCheck high sensitive is a microarray-based test kit for the highly sensitive qualitative detection of eight animal species in raw, cooked or fully preserved food products. With CarnoCheck high sensitive 8 animal species are detected in a single analysis with a sensitivity of 0.1%. fluorescent labels and buffer to amplify and label the species-specific DNA of all 8 animal species present in the sample during the PCR reaction. Species differentiation is accomplished by the hybridization of the fluorescently labeled PCR products to the CarnoCheck high sensitive microarrays. Each microarray contains replicates of species specific probes and a sophisticated set of internal and external controls making CarnoCheck a robust and reliable DNA based detection system. Even more product safety is added due to the automatic digital evaluation and report generation, which practically excludes false-positive and falsenegative results. The CarnoCheck high sensitive workflow is very user-friendly due to the development of a complete protocol from sample preparation to result analysis with minimized hands-on steps and time. Furthermore, 6 or 12 analyses can be done in parallel on one chip, which leads to an effective throughput with no extra hands-on time. The CarnoCheck high sensitive analysis starts by extracting the genomic DNA from a food sample using the Fast Extraction Kit (REF ). The Fast Extraction protocol is exceptionally quicking and user-friendly: only 6 working steps, 5 min of hands-on time and 25 min incubation time. The minimization of hands-on steps effectively reduces the contamination risk during the extraction procedure. After extraction, Taq Polymerase is added the CarnoCheck high sensitive PCR Mastermix, which contains an internal PCR control, primer, CarnoCheck high sensitive DNA-Chip 2

3 Working Schedule for CarnoCheck high-sensitive 1 Sample Collection 2 Fast Extraction 3 PCR 4 Hybridisation Incubation time: - Incubation time: 25 min Incubation time: 3,5 h Incubation time: 20 min 100 mg of a homogenous food sample is collected The newly developed Fast Extraction protocol minimizes the steps and hands-on time necessary for sample preparation for PCR. The PCR protocol allows a highly sensitive detection of animal species in raw, cooked or processed food samples. The performance of the PCR is monitored by the PCR Control. Each species is detected by a specific DNA probe present in five replicates. The hybridization procedure is controlled via an onspot hybridization control. Detectable species Animal species detectable with CarnoCheck high sensitive donkey (Equus Asinus) beef (Bos taurus) chicken (Gallus Gallus) sheep (Ovis Aries) horse (Equus Caballus) pork (Sus Scrofa) turkey (Meleagris Gallopavo) goat (Capra Hircus) 3

4 Total Time Hands-on time: 25 min Incubation time: 4,5 h 5 Wash & Dry Incubation time: 2 min Unbound DNA is removed in the washing steps. The chips are dried by centrifugation. 6 Scanning & Analysis Hands-on time: - Incubation time: 10 min The CarnoCheck high sensitive chip is scanned, analysed and evaluated using the CheckScanner and CheckReport - Software. A report is created that clearly indicates the presence or absence of one or more of the animal species detectable. 4

5 CarnoCheck high-sensitive at a glance Fast Extraction minimized hands-on time 6 hands-on steps, 5 min hands-on time and 25 min incubation time reduced contamination risk during the extraction procedure High Sensitivity Sensitivity 0.1% (w/w total product) Broad Food Product Range meat, mixtures of meat, sausages, soups, bouillon cubes, cheese, yogurt, butter, curd, lasagna, gelatin, cat or dog food products for raw, cooked or fully preserved food products Effective g g simultaneous detection of 8 species in a single analysis 1 multiplex PCR reaction + hybridization on DNA chip parallel processing of 6 or 12 samples on one DNA chip complete kit with DNA chips and solutions for 30 or 60 investigations Reliable PCR and on-spot hybridization controls CarnoCheck high sensitive User-friendly Minimal hands-on time: 25 min for the complete analysis of 8 species Total analysis time (inclusive incubation time) < 5 h Rapid digital evaluation and archiving of the results by by CheckReport Software CarnoCheck high-sensitive order information Item Nº Description Packaging CarnoCheck high sensitive Kits CarnoCheck high sensitive 30 tests CarnoCheck high sensitive 60 tests Fast Extraction 60 extractions CarnoCheck Equipment Starter Package CheckScanner incl. PC system and CheckReport Software ocheck hybridisation chamber with slideholder ocheck handle for slideholder ocheck washbox 2 5

6 For further information, please visit our website or contact us: Germany (Main office) Greiner Bio-One GmbH Phone Fax China Greiner Bio-One Suns Co., Ltd. Phone Fax Japan Greiner Bio-One Co. Ltd. Phone Fax Thailand Greiner Bio-One Thailand Ltd Phone Fax Austria Greiner Bio-One GmbH Phone Fax France Greiner Bio-One SAS Phone Fax office@fr.gbo.com Netherlands Greiner Bio-One B.V. Phone Fax info@nl.gbo.com United Kingdom Greiner Bio-One Ltd. Phone Fax info@uk.gbo.com Belgium Greiner Bio-One BVBA/SPRL Phone Fax info@be.gbo.com Brazil Greiner Bio-One Brasil Phone Fax office@br.gbo.com Hungary Greiner Bio-One Hungary Kft. Phone Fax office@hu.gbo.com India Greiner Bio-One INDIA Pvt., Ltd. Phone Fax info@gboindia.com Spain VACUETTE Espana S.A. Phone Fax info@vacuette.es Switzerland Greiner Bio-One VACUETTE Schweiz GmbH Phone Fax office@ch.gbo.com USA Greiner Bio-One North America Inc. Phone Fax info@us.gbo.com F Rev. 00, e