AGH 330. Fundamental Plant Biotechnology

Transcription

1 AGH 330. Fundamental Plant Biotechnology Credit Unit : 3(2-1)(2 lecture-hours: 2x 50 minutes; 1x Lab. practice: 3x 50 mnts) Semester : 5 Prerequisite : - Lecturer : Dr. Ni Made Armini Wiendi, MS (Course Coordinator) Dr. Agus Purwito, MSc Agr Prof. Dr. Bambang S Purwoko, MSc Prof Dr Sudarsono, MSc Dr. Darda Effendi, MS Dr. Awang M., MS Dr. Sintho W.A, MS Number of students : students in one class (4 parallel classes) 5-20 students in international class The important of course Fundamental Plant Biotechnology course learn about basic biotechnology in plant to support agriculture. Students in Department of Agronomy and Horticulture or in other Department in Bogor Agricultural University need to know about the simple technic in plant biotechnology for in vitro plant propagation, in vitro plant breeding, in vitro secondary metabolites production, and in vitro plant preservation. In this course will discuss about biotechnology concept and technique in tropical crops as a model. Fundamental biotechnology remain, as always, production of goods and services that are needed and can be provided with safety and reasonable Course descriptions This course gives students about the concept of fundamental plant biotechnology and to provide examples of how these principles are put into operation, in in vitro plant propagation technique for plant production seedlings, in vitro breeeding for incresing genetic diversity and selection to become a new variety, and some athers technique in biotechnology.

2 General Learning Outcome After completing this subject, the student are able to explain the concept and give an example of plant biotechnology and have skill in biotechnology technique. Additionally, they are able to choose a sspecific technique in biotechnology to be applied for crops, plantation and horticultural plants. Specific Learning Outcome After completing this course, the students will be able to explain about concept and technique of fundamental plant biotechnology, in vitro plant propagation via organogenesis and embryogenesis, gene expresstion and in vitro plant breeding, in vitro secondary metabolites production, and in vitro plant preservation Organization of course topics The main goal of this course is to provide students with good knowledge and skills on fundamental plant biotechnology. The lecture will be started on concept and scope of plant biotechnology, definitions and utilization in agriculture development, especially in tropical crops. For the next topic about tissue culture for plant propagation via organogenesis and embryogenesis, plant growth hormone, Students also learn about in vitro breeding via somaclonal variation, chromosome mutation, gene transformation, in vitro plant preservation and in vitro secondary metabolites production. Teaching Learning Strategy Course topics will be delivered through interactive lecture format. In the first 50 minutes the lecturer will explain about the topis, continued to discussion for 25 minutes. In the rest of time, lecturer will make a resume of the topics. As an effort to increase students understanding about the topics, the sudents are asked to read a related journal paper and make a short review. This review will be collected to the lecturer in the next lecture

3 Schedule of Class Meeting (Lecture) Week Topics 1 Introduction of plant biotechnology: concept, scope, definition and utilization in agriculture development 2 Plant tissue culture 3 Media and Plant Growth Hormone in tissue culture 4 Organonesis and embryogenesis in in vitro plant propagation 5 Synthetic seeds (artificial seeds) 6 Protoplast culture 7 In vitro secondary metabolites production 8-9 Midterm exam 10 Somaclonal variation in plant tissue culture 11 Anther Culture 12 DNA and gene expression 13 Molecular marker 14 Chromosome mutation 15 Gene transformation and expression 16 In vitro plant preservation Final exam

4 Schedule of Lab Practice Week Topic of practice 1 Introduction 2 Topic 1. Tissue culture medium preperation 3 Topic 2. In vitro plant propagation in horticulture crops 4 Topic 3. In vitro sterilization explant 5 Topic 4. Callus induction in vitro 6 Topic 5. Potato micro tuber induction in vitro 7 Topic 6. Embryogenesis induction from endosperm cells 8-9 Midterm exam 10 Topic 7. Embryo rescue 11 Topic 8. Anthera culture 12 Topic 9. Aclimatition of planlet 13 Topic 10. Ex - vitro cuttings 14 Observation and discussion 15 Observation and discussion 16 Observation and discussion Major References: 1. Wattimena GA et al Biotechnology in Plant Breeding. IPB Press, ID 2. Wattimena GA et al Plant Biotecnology. IPB Press, ID 3. Chrispeels, MJ and David ES Plants Genes, and Crop Biotechnology. 2ed. Jone and Bartlett Pub., London 4. Chawla HS Introduction to Plant Biotechnology. Science Publishers, Inc. USA. 5. Islam, A S In Vitro Culture, Transformation and Molecular Markers for Crop Improvement. Science Publishers, Inc. USA 6. Kozai T., Afreen, F. And Zobayed SMA. Photoautotrophic (sugar- free medium) Micropropagation and Transplant Production System. Springer, The Netherland

5 7. Trigiano RN and Gray D Plant Tissue Culture, Development, and Biotechnology. CRC Press, USA 8. Jain SM, Brar DS, Ahloowalla, BS Somaclonal Variation and Induced Mutations in Crop Improvement. Kluwer Academic Publisher. USA Evaluations Students performance will be graded based on his/her achievements in examinations and some assignments as follows: Midterm exam : 35% Final exam : 35% Practice : 30%. Category of grades are as follows : Range of Score Grade 80 A 75 x 79.9 AB 70 x 74.9 B 65 x 69.9 BC 60 x 64.9 C 55 x 59.9 CD 50 x 59.9 D <50 E