Biorefineries: the role of microbiology and biotechnology

Transcription

1 3 rd Workshop CLOSPORE Wageningen Food and Biobased Research, Wageningen, January 2017 Workshop organiser: Ana López Contreras 1

2 3 rd Workshop CLOSPORE 16 and 17 January 2018, Wageningen Food and Biobased Research Biotechnology applied to biorefineries for production of fuels from biomass (2 days). In this workshop, an overview of the use of biotechnology in processes for production of second generation biofuels and chemicals will be given. Visits to the BioPilot Facility in Delft and to the Wageningen Food and Biobased Research Innovation Plant are included in the workshop. Hosted by: Ana M. López-Contreras, WFBR and Marjon Wells-Bennink, NIZO Location address: Wageningen Food and Biobased Research P.O. Box 17, 6700 AA Wageningen, The Netherlands Bornse Weilanden 9, 6708 WG Wageningen Wageningen Campus l Building 118 (Axis) Number of participants: 15 researchers from CLOSPORE, supervisors, open to staff in WFBR Accommodation of participants: Ede, Hotel de Reehorst, ECTS points: 1 from Graduate School VLAG. A certificate will be given to the participants at the end of the workshop. 2

3 Draft program Day Registration Day 1 theme: Microorganisms for fuels, chemicals and high value compounds Welcome and introduction to the workshop - Ana López Contreras Improving butanol production by solvent-producing Clostridia by in-situ product removal Ana López Contreras, Wageningen Food and Biobased Research Production of food ingredients by fermentation Jeroen Hugenholtz, WFBR and UvA Emerging Biotechnologies for Biobased Aromatics Carmen Boeriu, Wageningen Food and Biobased Research Lunch, in the room Departure to Bio Pilot Facility (Delft) Visit to BPF Back to Ede De Reehorst Dinner at De Reehorst, including speakers 3

4 Day Introduction to Day 2 Theme: Metabolic and genome engineering for industrial processes Sustainable production of bio-n-butanol: from lab bench to commercial plant Holly Smith, Green Biologics Overview of the development of bioprocess in the context of a biorefinery Nicolas Lopes Ferreira, IFPEN Break The effect of spo0a modifications on the endospore formation and solvent production in solventogenic Clostridium species Sasha Atmadjaja Solvent production in a sporulation deficient Clostridium mutant Mamou Diallo Genetic tool development and sporulation mechanisms in thermophilic clostridia Joyshree Ganguly Metabolic engineering of thermophilic clostridia for production of dicarboxylic acids Jeroen Koendjbiharie Lunch CRISPR-Cas from exploration to exploitation John van der Oost, Wageningen University Solutions for more efficient microbial fermentations Astrid Mars, Wageningen Food and Biobased Research Break Pitches ESRs Closing remarks Visit to facilities WFBR for ESRs 4

5 ESRs pitches and project titles Time Location : Food and Biobased Research, Wageningen Unveiling bacterial sporulation processes in order to optimise a targeted cancer drug delivery system - Raquel M Rodrigues Screening, identification and characterization of novel sporulation genes using Conditional Transposon Mutagenesis - Rubab Rizvi Genetic diversity of Clostridium perfringens in relation to spore heat resistance Kristian Korellud Growth kinetics, spore properties and niche adaptation in Clostridium perfringens Matthew Callaghan Towards a safe Clostridium botulinum strain: CRISPR/Cas9 genome editing and strain characterization Daphne Groothuis Implementation of CRISPR-Cas gene editing system in Group II Clostridium botulinum Maria Nowakowska Understanding the regulation of sporulation and toxin production in Clostridium botulinum Ines Portinha Role of the Ser/Thr kinase PrkC in the physiology of Clostridium difficile Elodie Cuenot CotX, a Clostridium difficile spore protein required for the assembly of the surface layers Carolina Feliciano Two paralogous forespore-specific proteases required for sporulation in Clostridium difficile Amaury Fasquelle Role of a novel type of cysteine protease with an atypical response regulator domain in the assembly of the spore surface in Clostridium difficile Eleonora Marini Genetic tool development and sporulation mechanisms in thermophilic clostridia Joyshree Ganguly Solvent production in a sporulation deficient Clostridium mutant Mamou Diallo The effect of spo0a modifications on the endospore formation and solvent production in solventogenic Clostridium species Sasha Atmadjaja Metabolic engineering of thermophilic clostridia for production of dicarboxylic acids Jeroen Koendjbiharie 5

6 List of Clospore ESR s Raquel Mesquita Rodrigues, ESR 1 (UNOTT): mbzrsm@exmail.nottingham.ac.uk Syeda Rubab Rizvi, ESR 2 (UNOTT): msxsrr@exmail.nottingham.ac.uk Kristian Kollerud, ESR 3 (NIZO): kristian.kollerud@nizo.com Matthew Callaghan, ESR 4 (NIZO): matthew.callaghan@nizo.com Maria Maria Nowakowska, ESR 5 (UH): maria.nowakowska@helsinki.fi Ines Portinha, ESR 6 (UH): ines.portinha@helsinki.fi Joyshree Ganguly, ESR 7 (CORBION): joyshree.ganguly@corbion.com Jeroen Koendjbiharie, ESR 8 (CORBION): jeroen.koendjbiharie@corbion.com Amaury Fasquelle, ESR 9 (ITQB-UNL): amaury@itqb.unl.pt Eleonora Marini, ESR 10 (ITQB-UNL): eleonora@itqb.unl.pt Carolina Alves Feliciano, ESR 11 (IP): carolina.alves-feliciano@pasteur.fr Elodie Cuenot, ESR 12 (IP): elodie.cuenot@pasteur.fr Aretha Nessia Atmadjaja, ESR 13 (GBL): Sasha.Atmadjaja@greenbiologics.com Mamou Diallo, ESR 14 (WUR): mamou.diallo@wur.nl Daphne Groothuis, ESR 15 (UNOTT): mbzdg@exmail.nottingham.ac.uk 6