Pre Ph.D. (BIOTECHNOLOGY)

Transcription

1 FACULTY OF LIFE SCIENCES SYLLABUS FOR Pre Ph.D. (BIOTECHNOLOGY) Examinations: GURU NANAK DEV UNIVERSITY AMRITSAR Note: (i) Copy rights are reserved. Nobody is allowed to print it in any form. Defaulters will be prosecuted. (ii) Subject to change in the syllabi at any time. Please visit the University website time to time.

2 1 Scheme of Courses for Pre Ph.D. Course in Biotechnology Course Code C/E/I Course Name Credits Total Credits L T P LSL901* C Research Methodology BTL902 C Structural Biology & Bioinformatics BTL903 C Cell and Molecular Biology BTL904 C Advances in Biotechnology BTS905 C Seminar I Interdisciplinary Course (Students may do from any other Department) Total Credits * Common for Life Sciences

3 2 LSL901 - Research Methodology Credits Basic Concepts: Research process, problem identification, research designs, informal experimental designs. Completing randomised design, randomized block design, latin square design, factorial designs 2. Sampling designs: Random sampling, complex random sampling, non-probability sampling, measurement and scaling techniques. Data collection. 3. Processing and analysis of data: Central tendency, dispersion, asymmetry, correlation, regression analysis, multiple correlation and regression, partial correlation, association, index numbers, time series. 4. Sampling and testing of hypothesis: Concept of probability, probability distribution, Normal, Poisson, χ-square, t-test. Sampling distribution, central limit theorem, Sandler's A-test, standard error, population mean, population proportion, sample size, confidence intervals, null hypothesis and alternative hypothesis, level of significance, two tailed and one tailed tests, Z-test, t-test, x 2 -test, F-test, testing of correlation coefficients, ANOVAone way ANOVA, two way ANOVA Tukey's HSD. 5. Non-parametric tests: Sign test, Fisher-Irwin test, Mc Nemer test, Wilcoxon Mali test, Wilcoxon, Mann-Whitnery test, Kruskal-Wallis test, one sample runs test. Spearman's rank correlation, Kendall's coefficient of concordance. 6. Multivariate analysis: Multiple regression, multiple discriminant analysis, multiple analysis of variance, canonical correlation analysis, Factor analysis cluster analysis, path analysis. Computational techniques. 7. Survey of literature: The students will be required to review literature in their respective disciplines and submit an assignment for evaluation. References: 1. Kothari, C.R. (2004). Research Methodology: Methods and Techniques, New Age International Publishers, New Delhi. 2. Arya., P.P. and Pal, Y.(2001) Research Methodology in Management: Theory and Case Studies. Deep and Deep Publishers Pvt. Ltd., New Delhi.

4 3 BTL902-Structural Biology and Bioinformatics Credits Bioinformatics Biological databases, tools for DNA and protein sequence and structure analysis, Sequence alignment Protein structure and Function structure-function relationship using case studies, structure alignment and classification, structure databases, visualizing structure Tools for Structure Determination Structure prediction and determination: X-ray crystallography, NMR and CD, Protein secondary and tertiary structure prediction tools Data Base Management System and Data Mining DBMS Concepts: Data Abstraction, Database System Architecture, Schemas and Subschemas, Data Modeling using Entity Relationship, Hierarchical and Network Models, Normalization- First, Second, Third and BCNF normal forms. Introduction to Data mining: Classification, Clustering, Data Warehousing, Role of Data Mining in Bioinformatics References: 1. Date, C.J. (2003) An Introduction to Database Systems, 8th Ed. Addison Wesley. 2. Mount D. W. (2004). Bioinformatics & Genome Analysis. Cold Spring Harbor Laboratory Press. 3. Eidhammer I., Jonassen I. and Taylor W. R. (2004). Protein Bioinformatics: An algorithmic approach to sequence and structure analysis. Mathematics. 4. Bourhe P. E. and Weissig H. (2003). Structural Bioinformatics (Methods of structural Analysis). Wiley-Liss. 5. C. Braden and C. Tooze (1991). Introduction to Protein Structure Garland Publishing Inc., New York. 6. Sambrook, J., Fritsch, E.F. and Maniatis, T. (2000). Molecular Cloning: A Laboratory Manual,. Cold Spring Harbor Laboratory Press, New York. 7. Recent Research articles from Journals

5 4 BTL 903 -Cell and Molecular Biology Credits Stem Cell Biology : Stem cells- definition, classification and sources: embryonic stem cells, adult stem cells and mesenchymal stem cells; stem cells differentiation; Stem cells cryopreservation; clinical applications of stem cells; Mammalian Nuclear Transfer Technology; Stem cell based therapies and ethical considerations. Cancer Biology : Eukaryotic cell cycle, regulators of cell cycle progression, oncogenes, DNA repair defects and genomic instability in cancer cells, epigenetics and cancer, cancer genome, cell line based evaluation of anticancer agents, apoptosis and therapeutic aspects. Gene silencing techniques and applications: Overview of different strategies for gene silencing, RNA interference, construction of RNA interference vectors, Applications of RNA interference in crop improvements Proteomics and Genomics Separation of proteins by 2-D Electrophoresis, identification of protein by MALDI-TOF MS, Protein microarrays Fermentation Technology Down Stream Processing: Introduction, down stream processing, centrifugation and industrial centrifuges, filtration, precipitation, aqueous two phase system separations, liquid-liquid extractions, super-critical fluid extraction, Effluent treatments. References: 1. Spier, R.R. and Grifftths, J.B. (1994). Animal Cell Biotechnology, 6th Ed., Academic Press, London. 2. Robert L. and other (2009).Essenti als of Stem Cell Biology. 2nd Ed. Academic Press, London. 3. Recent Research articles from Journals 4. Principles of Fementation Technology, Peter F. Stanbury; A. Whitaker; S.J. Hall. Aditya Books (P) Ltd. New Delhi 2009

6 5 BTL 904 -Advances in Biotechnology Credits Introduction to Tissue Engineering; role of scaffolds and growth factors; biomaterials and tissue engineering; organotypic and histotypic cultures; tissue replacement therapy; imaging tissue constructs Plant Cell Transformation: Strategies for plant cell transformation, regulation of expression of transgenes, selection of transgenic cells, screenable markers, validation of transgenes. Plant Tissue Culture & Medicinal Biotechnology : Medicinal natural products and their importance, Role of plant cell and tissue culture in medicinal plants, Genes and pathway discoveries, Metabolic engineering of alkaloid biosynthesis, Transport, accumulation and storage of secondary metabolites, Biotechnological approaches for quality control of medicinal plants Microbial Technology: Molecular approaches to study microbial diversity, the human microbiome project, comparative genomics, gene ontology and microbial sequencing projects, functional metagenomics for microbial enzyme discovery and bioactive metabolites from microbes. Bioprocess Engineering Bioreactors and Bioprocesses: Introduction, designing of bio-reactors, batch bioreactor design and its kinetics, continuous stirred tank bioreactors designing and its kinetics, steady state kinetics, fed batch bioreactors and its kinetics, quasy steady state condition, plug flow bioreactors and its kinetics.