SULTAN QABOOS UNIVERSITY COURSE OUTLINE PROGRAM: Biotechnology. Field or Work Placement

Transcription

1 SULTAN QABOOS UNIVERSITY COURSE OUTLINE PROGRAM: Biotechnology 1. Course Code BIOL Course Title Tissue Culture 3. Credits 3 4. Pre-requisite Course(s) BIOL Co-requisite Course(s) 6. Equivalent Course(s) 7. Incompatible Course(s) 8. Course Category University Requirement University Elective College Requirement Department Requirement Specialization Requirement Other (specify): College Elective Department Elective Specialization Elective 9. Course Owner College: Department: Biology 10. Course Type Lecture Lecture/Lab Lecture/Seminar Lecture/Tutorial Tutorial 11. Language of Instruction English Field or Work Placement Seminar Workshop Lecture/Studio Lecture/Lab/Tutorial or Seminar Laboratory (Practical) Studio Internship Project 12. Course Description This is an advanced course dealing with the concepts of cell and tissue culture and applications in biotechnology. The topics include plant and animal cell culture, tissue and organ culture, micropropagation, organogenesis, somatic embryogenesis, in vitro haploid production, somaclonal variation, protoplast isolation, culture and fusion, and genetic transformation in crops. 13. Teaching/Learning Strategies 14. Assessment Components and Weight [%] Quizzes 10 Practical 20 Other (specify): Homework assignments Project In-term examination(s) 30 Final examination Grading Method A-F Scale Pass/Not passed 16. Textbook(s) and Supplemental Material 1) Plant Biotechnology by H.S. Chawla 2) Stem Cells by A. M. Wobus (2006) 17. Matching Course Objectives with Program Outcomes and SQU Graduate Attributes SQU Graduate Attributes Page 1

2 A. SQU graduates should be able to: 1. apply the knowledge and skills relevant to the specialization 2. communicate effectively and use information and communication technologies 3. critically analyze complex information and present it in simple clear manner B. SQU graduates possess 1. interpersonal communication skills and alignment with culture of international labour market to assist them in practical life and in living successfully 2. skills and motivation for independent learning and engagement in lifelong learning and research 3. work ethics and positive values, and intellectual independence and autonomy 4. teamwork skills and display potential leadership qualities C. SQU graduates should relish good citizenship qualities, be conscious of their national identity and be socially responsible, engage in community affairs and be mindful of contemporary issues # Intended Student Learning Outcome /Course Learning Objective Apply the principles of plant cell and tissue culture in the scientific and commercial applications of in vitro biotechnology. Select the methodologies of micropropagation in crop plant improvement. Illustrate callus induction, organogenesis, somatic embryogenesis and synthetic seed technology Demonstrate the totipotency of callus; produce somatc embryoids and organogenesis and recognize their commercial applications. Outline an introduction to animal tissue culture, regulatory mechanasims and clinical setting Define the Initiation of Culture, propagation, cell growth, source of contamination, passaging and generation number of cell lines Review the process of cell division, adhesive factors, the content of a culture media Describe stem cell establishment, self renewal/pluripotency and extrinsic and intrinsic factors Relevant Program Outcome(s) Appendix-B. 1,2,3 Appendix-B. 1,2,3 Appendix-B. 1,2,3 Appendix-B 3 to 6 Appendix-B 6 Appendix-B 2 to 4 Appendix-B 2 to 5 Applicable Attribute(s) 16. Student Responsibilities It is the student s responsibility to know and comply with all University Academic Regulations relevant to participation in this course. These regulations specifically include attendance requirement and students` academic code of conduct. For attendance, it is the student s responsibility to be punctual and to attend all classes. Students are expected to perform their work with honesty and avoid any academic misconduct, which is defined as the use of any dishonest or deceitful means to gain some academic advantage or benefit. This can take many forms, including but not limited to, the following: copying, plagiarism, collusion and forging documents. For full details, please refer to the Undergraduate Academic Regulations and to the Student Academic Misconduct Policy. Additionally, this course requires that you: Page 2

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4 COURSE INFORMATION Course Code BIOL5132 Course Title Tissue culture Semester/ Year SP/2018 Section(s) 10 & 11 Day, Time, and Place Course Coordinator Dr. Sardar Farooq and Dr. Senan Baqir Office Location Room 2032 and 2039 Office Hours 12-1 PM Sunday Office Tel. Ext Tentative Schedule Week Lecture # Topic/Material to be covered Assessment 1 1 & 2 Introduction and applications of Plant Tissue culture. 2 3 & 4 Plant Tissue culture Media & Types of Cultures 3 5 & 6 Totipotency & Cytodifferentiation, Meristem culture & Micropropagation 4 7 & 8 Organogenesis and Nonzygotic embryogenesis & Artificial seeds 5 9 & 10 Production of Haploids (Anther/Pollen culture) 6 11 & 12 Protoplast culture & Somatic hybridization Test & 14 Cell Suspension culture & Secondary metabolite production 8 15 & 16 Tissue culture introduction 9 17 & 18 Principles of Animal Tissue Culture & 20 Tissue Structure and Molecules & 22 Introduction to Stem Cells Test & 24 Self Renewal and Pluripotency Final 17 Page 4

5 Section Instructor APPENDIX A: INSTRUCTORS OF MULTIPLE SECTIONS Day, Time, and Place Office Location and Extension Office Hours Page 5

6 APPENDIX B: ADDITIONAL INFORMATION 1. Students will be able to demonstrate their knowledge in general biology concepts as well as knowledge in biotechnology. 2. Student will possess the technical background knowledge and skills required to conduct research in biotechnology, cell/ molecular biology. 3. Students are able to use knowledge of their subject to understand and solve problems related to their specialization. 4. Ability to think critically and independently also adopt scientific thinking throughout their careers. 5. Student will demonstrate ability to communicate (verbally or in writing) their knowledge to various levels of audience with regard to the field of biotechnology and Biology in general. 6. Students will demonstrate awareness of occupational health and safety issues as they relate to the workplace, display a high level of ethical conduct in the practice of their profession and have the ability to work well independently and in a team. Page 6