Genetic Testing Tool STH-PAS

Transcription

1 Technical Information ( ) Genetic Testing Tool STH-PAS ~ Easily used by anyone, anywhere ~

2 What is STH-PAS? STH(Single-stranded Tag Hybridization) Method: 1Conduct PCR amplification of target DNA using a labeled primer and a primer with single-stranded tag DNA 2Spread the amplified solution on the membrane strip without heat denaturation (singlestranded) 3Trap the amplified PCR product using strong hybridization reaction between singlestranded complementary tag DNA and single-stranded tag immobilized in a line shape 4Labeling agent for the trapped amplified PCR product enables the line visualized by coloring 5A new genetic testing method to determine the presence or absence of the target DNA in a sample PAS(Printed-Array Strip): Membrane strip used in the above STH method with several kinds of complementary DNA tag immobilized in a line Basic width of the line is 0.5mm which enables the visualization (March 2014:The STH method patented as a new genetic testing method)

3 Contents of STH-PAS Genetic Testing Kit PAS PAS(Printed Array Strip) Provided bytba Corp. C-PAS(4) C-PAS(8) C-PAS(12) STH Reagent Provided by reagent manufacturers PCR primer w/tag Enzyme Nucleic acid, etc Visual judgment function Target identification function Genetic Testing Kit STH Reagent Chromatographic Develop. Solution Chromatographic Development Solution Provided by chemical product manufacturers Develop. solution Coloring particle Line coloring function

4 Test Procedures (Example of multiple testing of 4 targets) Primer set w/tag for 4 targets Tag1 For target1: Tag2 For target2: For target3: For target4: Extracted DNA Tag3 Tag4 STH reagent Primer with tag (Mixture of 4 sets) Enzyme and others Testing for the presence of the 4 targets Coloring of amplified product Amplified product with tag is colored. Exists only target2 Complementary tag DNA PCR amplified product (with tag and biotin) PAS detection PAS Line coloring Position marker Capturing and coloring by complementary reaction between tag2 and 2. Sample Avidin colored beads Visual judgement PCR amplification process (about 30min.) Develop. solution Chromatographic development process (about 10min. in room temperature)

5 Comparison with the conventional nucleic acid chromatography method Heat denaturation process is not required, since the single-stranded DNA tag is captured. PAS(detection strip) can be used in any testing. Standard Method STH Method PCR amplification PCR amplification Heat denaturation Heat denaturation process not required! Hybridization Hybridization Conflict

6 Advantages of STH-PAS Any special knowledge or skilled technology is not required. Simultaneous testing(multiplex) of several kinds of targets can be done. PAS can be used for various kinds of testing. Other advantages are as follows: (Comparison with benchmark technology in each area) Area Method Sensitivity (copy/10μl) Performance Accuracy (SNP detection) Time (min.) Reagent/Test aprice Equipment cost Research PCR Electrophoresis Around 10² Detectable PCR $1-5 $3,500 PCR(STH) PAS Around 10² Detectable PCR $1-5 Around $2,000 Clinical Immunochromatography Around 10⁴ Undetectable Around $10 - PCR(STH) PAS Around 10² Detectable PCR Around $5 Around $2,000 Testing Lab. q-pcr Around 10² Detectable PCR only Around $15 $30,000 PCR(STH) PAS Around 10² Detectable PCR Around $5 Around $2,000

7 Expected Application of Genetic Testing (using PAS) Research, healthcare and others Food inspection field Mobile equip. Genetic research - Marker search, etc. Pet diagnosis, etc. - Disease, genomic breeding Development Concept:Genetic testing technology performed by anybody anywhere Sample PCR amplification STH reagent PAS Chromato. Develop. Infecting bacteria test - Food poisoning and aquaculture disease bacteria Medical diagnostic field Varieties Appraisal - Meat and agricultural products Infection diagnosis (Drug resistance, type classification) - Pathogen such as mycobacterium tuberculosis, malaria, dengue fever Chromato. detection - Virus such as HPV, HIV, Hepatitis V, avian influ.v - Food poisoning, sexually transmitted disease, sepsis, oral infection Deployment to Developing countries Companion diagnosis - Various anti-cancer agent, arthritis therapeutic agent, etc. - Drug metabolism genetic testing