For focused group profiling of human endometrial cancer related genes expression

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1 GeneCopoeia TM Expressway to Discovery ExProfile TM Human Endometrial Cancer Gene qpcr Array For focused group profiling of human endometrial cancer related genes expression Cat. No. QG067-A (1 x 96-well plate, Format A) Cat. No. QG067-B (1 x 96-well plate, Format B) Cat. No. QG067-C (1 x 96-well plate, Format C) Cat. No. QG067-D (1 x 96-well plate, Format D) Cat. No. QG067-E (1 x 96-well plate, Format E) Available as 1 set or 6 sets. Each set contains 82 unique gene primers deposited in one 96-well plate. Introduction The ExProfile human endometrial cancer gene qpcr array profiles 82 human genes to aberrantly expressed human genes involved in human endometrial cancer. These genes are carefully chosen for their close cancer correlation based on a thorough literature search of peer-reviewed publications. Abnormal gene expression is often observed in cancer development and progression. The ExPofile human endometrial cancer gene array allows researchers to study the cancer-related genes to gain understanding of their roles in endometrial cancer pathogenesis. QG067 plate 01: 82 unique gene PCR primer pairs Shipping and storage condition Shipped at room temperate Stable for at least 6 months when stored at -20 C Array format GeneCopoeia provides five qpcr array formats (A, B, C, D, and E) suitable for use with the following realtime cyclers. Important note: Upon receiving, please check to make sure that the correct array format was ordered to ensure the compatibility with your qpcr instrument. Plate format Instrument provider qpcr instrument model A B C D E Applied Biosystems Applied Biosystems Bio-Rad Laboratories Bio-Rad Laboratories Roche Applied Science 5700, 7000, 7300, 7500, 7700, 7900HT (Standard 96-well block), ViiA TM 7 (Standard 96-well block) 7500 (Fast block), 7900HT (Fast block), StepOnePlus TM, ViiA TM 7(Fastblock) icycler iq,myiq,iq 5 CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 LightCycler 480 (96-well block) 1

2 Quality Control 1. Each pair of primers in the ExProfile gene qpcr array has been experimentally validated to yield a single dissociation curve peak and to generate a single amplicon of the correct size for the targeted gene. 2. The positive PCR controls (PCR) have been verified to amplify a single amplicon of the correct size with Ct values around 20±2. 3. The Spike-in reverse transcription controls (RT) have been verified to amplify a single amplicon of the correct size with Ct values around 20±3. 4. R 2 > 0.99 was observed for high inter/ intra-array reproducibility. Materials required but not provided All-in-One TM First-Strand cdna Synthesis Kit All-in-One TM qpcr Mix Total RNA extraction kit (RNAzol RT RNA extraction reagent is recommended) DNase/RNase free tips, PCR reaction tubes, 1.5 ml microcentrifuge tubes 5 ml and 10 ml graduated pipettes, beakers, flasks, and cylinders 10 μl to1,000μl adjustable single channel micropipettes with disposable tips 5 μl to20μl adjustable multichannel micropipette, disposable tips, and reservoir qpcr instrument, compatible with gene qpcr arrays ordered Array layout Figure1. Illustration of QG067 plate 01 Gene primer pairs: 84 wells (A row to G row) are designated for a real-time PCR assay for genes (see the primer list). HK1-6: Six pre-deposited housekeeping gene (HK1-6) primer pairs, which can be used as endogenous positive controls as well as for array normalization. GDC: Genomic DNA controls, which can be used to specifically detect genomic DNA contamination with a high level of sensitivity. RT: Spike-in reverse transcription controls, which can be used to monitor the efficiency of the RT reactions. These pre-deposited primer pairs specifically amplify the cdna template reversed transcribed from the spike-in control RNA in the sample. PCR: Positive PCR controls, which are used to verify the PCR efficiency by amplifying the predeposited DNA template with its specific pre-deposited primer pairs. 2

3 Gene Primer List Plate Position Catalog No. of Primer Accession No. of Gene Symbol QG A01 HQP NM_ CYP17A1 QG A02 HQP NM_ CYP19A1 QG A03 HQP NM_ TP53 QG A04 HQP NM_ PTEN QG A05 HQP NM_ ESR1 QG A06 HQP NM_ CYP1A1 QG A07 HQP NM_ MSH2 QG A08 HQP NM_ MLH1 QG A09 HQP NM_ KRAS QG A10 HQP NM_ AR QG A11 HQP NM_ CYP1B1 QG A12 HQP NM_ PGR QG B01 HQP NM_ MSH6 QG B02 HQP NM_ COMT QG B03 HQP NM_ PIK3CA QG B04 HQP NM_ SHBG QG B05 HQP NM_ BRAF QG B06 HQP NM_ PPARG QG B07 HQP NM_ MDM2 QG B08 HQP NM_ CYP11A1 QG B09 HQP NM_ XRCC3 QG B10 HQP NM_ XRCC1 QG B11 HQP NM_ VEGFA QG B12 HQP NM_ SULT1A1 QG C01 HQP NM_ SULT1E1 QG C02 HQP NM_ CCND1 QG C03 HQP NM_ PMS2 QG C04 HQP NM_ HSD17B1 QG C05 HQP NM_ ESR2 QG C06 HQP NM_ AKT1 QG C07 HQP NM_ ERCC1 QG C08 HQP NM_ ADRB3 QG C09 HQP NM_ CASP7 QG C10 HQP NM_ CASP3 QG C11 HQP NM_ NCOA3 QG C12 HQP NM_ TNKS2 QG D01 HQP NM_ CASP8 QG D02 HQP NM_ XRCC5 QG D03 HQP NM_ XPC QG D04 HQP NM_ UGT2B7 QG D05 HQP NM_ UCP2 QG D06 HQP NM_ TYMS QG D07 HQP NM_ TGFB1 3

4 4 QG D08 HQP NM_ TERF2 QG D09 HQP NM_ TERF1 QG D10 HQP NM_ STAR QG D11 HQP NM_ BRCA2 QG D12 HQP NM_ SRD5A2 QG E01 HQP NM_ NOD2 QG E02 HQP NM_ CCL2 QG E03 HQP NM_ RNASE3 QG E04 HQP NM_ ENOSF1 QG E05 HQP NM_ ATR QG E06 HQP NM_ TLR9 QG E07 HQP NM_ PIK3C2A QG E08 HQP NM_ ABCB1 QG E09 HQP NM_ PAK3 QG E10 HQP NM_ NME1 QG E11 HQP NM_ MUC1 QG E12 HQP NM_ MTHFR QG F01 HQP NM_ MMP9 QG F02 HQP NM_ MMP3 QG F03 HQP NM_ MMP2 QG F04 HQP NM_ MMP1 QG F05 HQP NM_ LEPR QG F06 HQP NM_ LEP QG F07 HQP NM_ IRS1 QG F08 HQP NM_ IGFBP3 QG F09 HQP NM_ IGFBP1 QG F10 HQP NM_ IGF2 QG F11 HQP NM_ IGF1 QG F12 HQP NM_ POT1 QG G01 HQP NM_ ERCC4 QG G02 HQP NM_ ERCC2 QG G03 HQP NM_ EGFR QG G04 HQP NM_ ACE QG G05 HQP NM_ CTNNB1 QG G06 HQP NM_ CHEK2 QG G07 HQP NM_ NOD1 QG G08 HQP NM_ TP73 QG G09 HQP NM_ OGG1 QG G10 HQP NM_ ERCC5 QG G11 QG G12 QG H01 HGDC QG H02 HGDC QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A

5 QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR 5

6 Limited Use License Following terms and conditions apply to use of ExProfile TM Endometrial Cancer Gene qpcr Array (the Product). If the terms and conditions are not acceptable, the Product in its entirety must be returned to GeneCopoeia within 5 calendar days. A limited End- User license is granted to the purchaser of the Product. The Product shall be used by the purchaser for internal research purposes only. The Product is expressly not designed, intended, or warranted for use in humans or for therapeutic or diagnostic use. The Product must not be resold, repackaged or modified for resale, or used to manufacture commercial products or deliver information obtained in service without prior written consent from GeneCopoeia. This Product should be used in accordance with the NIH guidelines developed for recombinant DNA and genetic research. Use of any part of the Product constitutes acceptance of the above terms. Limited Warranty GeneCopoeia warrants that the Product meets the specifications described in the accompanying Product Datasheet. If it is proven to the satisfaction of GeneCopoeia that the Product fails to meet these specifications, GeneCopoeia will replace the Product. In the event a replacement cannot be provided, GeneCopoeia will provide the purchaser with a refund. This limited warranty shall not extend to anyone other than the original purchaser of the Product. Notice of nonconforming products must be made to GeneCopoeia within 30 days of receipt of the Product. GeneCopoeia s liability is expressly limited to replacement of Product or a refund limited to the actual purchase price. GeneCopoeia s liability does not extend to any damages arising from use or improper use of the Product, or losses associated with the use of additional materials or reagents. This limited warranty is the sole and exclusive warranty. GeneCopoeia does not provide any other warranties of any kind, expressed or implied, including the merchantability or fitness of the Product for a particular purpose. GeneCopoeia is committed to providing our customers with high-quality products. If you should have any questions or concerns about any GeneCopoeia products, please contact us at GeneCopoeia, Inc. GeneCopoeia, Inc Medical Center Drive, Suite 101 Rockville, MD (301) (866) inquiry@genecopoeia.com GeneCopoeia Products are for Research Use Only Copyright 2016 GeneCopoeia, Inc. Trademarks: GeneCopoeia, ExProfile, All-in-One, (GeneCopoeia Inc.); RNAzol RT (Molecular Research Center, Inc.); SYBR (Molecular Probes); icycler iq, MyiQ, iq 5, CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 (Bio-Rad); LightCycler (Roche);Trizol, ABI,ROX, ViiA TM,StepOnePlus TM (Life Technologies). QG