Magellan Bioscience Business and Capabilities. Applications of Marine Microorganisms. Overview of Collection and Process Development

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2 Overview Magellan Bioscience Business and Capabilities Applications of Marine Microorganisms Overview of Collection and Process Development

3 Magellan BioScience Group, Inc. Location: Tampa, Florida Founded: 1997 What do we do? o Natural products discovery company o Use novel technology to screen microbial products for industrial applications o Provide extracts and services based on clients needs

4 Business Models NRDO- No Research Development Only.. RIPCO/RIBCO- Royalty Income Biotechnology Company FIPCO/FIBCO- Fully Integrated Biotechnology Company Technology Platform (Research Only)- Rent/Sells technology Magellan - Hybrid

5 Clients Pharmaceutical Agrochemical Cosmetic MAGELLAN BIOSCIENCE Food and Flavor Animal Health Care Specialty Chemical Energy Internal Research MAGELLAN ANALYTIX

6 Internal Research Efforts Novel plant growth promoter induces growth more than 20% over controls and induces salt tolerance Anti-inflammatory/Anticancer product oil Nonsteroidal animal growth promoter Nutraceuticals: Pharmaceuticals: Urinary tract Health Irritable bowel Syndrome Abuse resistant technology

7 Magellan- Recent Developments MAGELLAN ANALYTIX Pharma Like Research (GLP) Nutraceuticals 21 CFR 111 Pharmaceuticals 21 CFR 211

8 Capabilities Fermentation Fractionation, Separations Isolation, Structure Elucidation Synthetic Modifications Microbial Biotransformation

9 Distribution of Marine Natural Products by Phylum Coelenterates 21% Molluscs 7% Tunicates 7% Echinoderms 3% Microorganisms & Phytoplankton 15% Sponges 38% Red algae 6% Brown algae 2% Green algae 1% Personal Communication from John Blount

10 As the number of compounds from terrestrial microbes decline Novel compounds from marine microbes increase...

11 Discovery Process for Novel Leads from Marine Natural Products

12 Microbial Dereplication (Select Subset of Cave Microorganisms)

13 Why Use Marine Microorganisms?? Untapped source of new products Robust and simple to culture Seasonably independent Environmentally benign Products are natural Sustainable, renewable, consistent, long-term supply of raw materials for fermentation Rapid scale-up and production Independent of Oil and Natural Gas Supplies DNA preserved for future manipulation

14 Microbial Metabolites Primary metabolites essential chemical compounds that play direct role in growth, development or reproduction Secondary metabolites nonessential for life sustaining activity Why are they important? They can be applied to various products as efficient active components due to their properties and functions Vitamins Antibiotics Uses Colorants Detergents

15 Classes of Products Produced by Marine Microorganisms Biosurfactants Pigments Enzymes Polysaccharides Proteins Antioxidants Peptides Fatty acids Bioadhesives UVA absorbers Polyhydroxyalkanoates

16 Terrestrial vs. Aquatic Terrestrial Biosphere Accessibility - Easy to obtain Number of new products is dwindling Marine Biosphere High potential Higher Risk Microorganisms account for 98% biomass Unique biodiversity - extreme environments

17 Bioprospecting Diverse locations such as the Caribbean, Hawaii, New England, Bermuda, Arctic and Antarctic etc. Deep ocean sediment Shallow coral reefs Water sampling Marine caves Adhere to guidelines of the 1992 Rio Treaty on Biodiversity Obtain informed consent from foreign countries prior to collecting Equitable agreement to share a percentage of profits Avoid being labeled biopirate

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20 Summary of Magellan s Microbial Extract Process

21 Collection and Culturing Divers given instructions Collect microorganisms associated with macroorganisms (sponges, tunicates, echinoderms, etc.) Samples serial diluted in proprietary medium Must be capable of surviving at room temperature

22 Identification and Cryopreservation Microbial Strain Purification Identification o Look for unique colony forming units Phenotypic characteristics Pigmentation Zone of inhibition, etc o Fatty Acid Fingerprinting check for duplicate samples Cost effective and just as good as DNA sequencing Cryopreservation

23 Libraries Isolates Over 10,000 cultureable marine microbes Over 500 endophytic fungal samples Extracts Over 500 extracts (macroorganism) Obtained from several geographic locations Biologically active compounds

24 Cultivation Process Active projects kept on shakers at room temperature. Multiple copies kept in different freezers All work is conducted in laminar-flow hoods

25 Freeze-drying Lyophilization Purpose dehydrate trays by removing water Result concentrated microbe & its products

26 Extraction Filtration Process Proprietary organic solvent extraction of lyophilized material Separates microorganisms from metabolites

27 Extract Plating Mother plates standard 96 wells Store for cryopreservation Ship to client

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