THE 8 TH INTERNATIONAL CONFERENCE ON BIOSCIENCE AND BIOTECHNOLOGY Biosciences and Biotechnology for a Sustainable Life

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1 THE 8 TH INTERNATIONAL CONFERENCE ON BIOSCIENCE AND BIOTECHNOLOGY Biosciences and Biotechnology for a Sustainable Life DENPASAR, SEPTEMBER 2017 ORGANIZED BY FACULTY OF AGRICULTURE UDAYANA UNIVERSITY

2 TIME SCHEDULE 8 th INTERNATIONAL CONFERENCE ON BIOSCIENCE AND BIOTECHNOLOGY Venue: Post Graduate Building (3 rd floor), Jl. PB. Sudirman, Denpasar Bali First Day: ICBB Conference (September 14 th, 2017) No Time Activities Committee (local time) Registration Opening Ceremony: MC a. Opening dance b. Speech by Chairperson of AOBBC c. Speech by KBI (Indonesian Biotechnology Consortium) d. Speech by Dean of Faculty of Agriculture, Udayana University e. Speech and official opening of ICBB by Rector of Udayana Keynote Speakers a. Prof. Takuya Marumoto b. Dr. Inez S. Loedin Invited Speakers a. Prof. Yoshihiro Natori b. Prof. Dr. Acram Taji c. Prof. Bambang Sugiarto d. Q & A Lunch Break and Pray Time Invited Speakers a. Prof. I G. P. Wirawan, Ph.D b. Dr Alex Johnson c. Q & A Parallel Session a. Agriculture 1 (pest and disease/plant protection) b. Agriculture 2 (agronomy and plant science) c. Agriculture 3 (fertilizer/soil science) Prof. Wirawan/ Prof Sachiko Takahi Prof. Misri Gozan Prof. Dr. Ir. I Nyoman Rai, M.S. Prof. Dr. dr. A.A. Raka Sudewi, Sp.S (K) Moderator Moderator Moderator Moderators 8 th ICBB 2017 i

3 d. Agriculture 4 (molecular biology, biochemistry, agriculture bio-engineering and health e. Agriculture 5 (soil microbiology/soil science) AOBBC meeting and dinner Alit Hotel Second Day: ICBB Conference (September 15 th, 2017) No Time Activities Committee (local time) Invited Speakers Moderator a. Dr. Kahar Muzakkar, S.Si b. Dr. Debbie S. Retnoningrum c. Dr. Ir. I Made Tasma, M.Sc d. Q & A KBI Meeting Invited Speakers Moderator a. Prof. Young Je you b. Dr. Eng. Himawan Tri Bayu, M.P c. Prof. Miyasaka d. Dr. Siswa Setyahadi e. Q & A Lunch Break and Pray Time Parallel Session Moderators a. Agriculture 1 b. Agriculture 2 c. Agriculture 3 d. Animal science and husbandry e. Special Lecture for Studying in Japan (Scholarship Scheme) Closing Ceremony a. Report and thank you note by Chairman of the Committe b. Closing Speech by Dean of Faculty of Agriculture MC Dr. Ir. I Dewa Putu Oka Suardi, M.Si Prof. Dr. dr. A.A. Raka Sudewi, Sp.S (K) 8 th ICBB 2017 ii

4 AGRICULTURE 6 No Name Institution/ Title Time Moderator 1 I Nyoman Wijaya*, Wayan Adiartayasa And Made Sritamin I Putu Sudiarta 2 Putu Wina Andriani Lestari *, Ida Ayu Astarini, Made Ria Defiani Agrobioecology Program Study, Faculty of Agriculture, Udayana University Phone Number * Wijayainyoman1956@Gmail.Com Biology Master Degree Study Program, Udayana University, Bali * winaandriani5@gmail.com The Molecular Detection Of Citrus Vein Phloem Degeneration (Cvpd) Pathogen (Liberobacter Asiaticus) In Diaphorina Citri Kuwayama (Homoptera : Psyllidae) And Other Insects Associated With Citrus Plant Virus Identification On Early Generation Seed Potatoes In Kembang Merta Village, Bali Deborah Margareth 1*, Ida Ayu Astarini 1, Martin Joni 1, I Gede Rai Maya Temaja 2 4 Wayan Adiartayasa, I N. Wijaya and I G. N. Bagus* 1 Biology Master Degree Study Program, Udayana University, Bali 2 Biotechnology Master Degree Study Program, Udayana University, Bali * deboramargareth48@gmail.com Faculty of Agriculture, Udayana University Kampus Bukit Jimbaran, Badung, Bali Weeds and Virus Identification of Potato Fields in Pancasari Village The Study of the Citrus Vein Phloem Degeneration with th ICBB 2017 xxi

5 TABLE OF CONTENTS TIME SCHEDULE SCHEDULE OF PARALLEL PRESENTATION Parallel Session Agriculture 1 (A1) Parallel Session Agriculture 2 (A2) Parallel Session Agriculture 3 (A3) Parallel Session Agriculture 4 (A4) Parallel Session Agriculture 5 (A5) Parallel Session Veterinary and Animal Husbandry 1 (V1) Parallel Session Agriculture 6 (A6) Parallel Session Agriculture 7 (A7) Parallel Session Agriculture 8 (A8) Poster TABLE OF CONTENTS Collaboration Project Between Udayana Univ. And Takino Filter Inc. For Environmental Restoration In Bali, Indonesia,2012~2016 T. Marumoto, N. Kohno and T. Simizutani i iii iii vi ix xii xv xviii xxi xxiv xxvii xxx xxxix 52 Use Of In Vitro Breeding Strategies In Development Of Plants 53 Acram TAJI Production Of Virus Free And Resistance Against Mosaic Virus Using Pathogen-Derived Resistance And RNA Interference Technology In Sugarcane* Bambang Sugiharto 54 Biocatalytic Removal Of Nitrate 56 Young Je Yoo, Professor Identification Of Novel Metal Homeostasis Genes In Bread Wheat And Applications In Plant Breeding Julien Bonneau, Jesse Beasley, Tonatiuh Sanchez-Palacios, Laura T. Moreno-Moyano and Alexander A. T. Johnson* Advanced Research On Agrobacterium Tumefaciens From Crown Gall Tumor Formation To Mediate Genetic Transfer To Plant Cells*Plant Cells* th ICBB 2017 xxxix

6 Bali Cattle Infected With Dermatophytosis In Sobangan Village, Badung Regency Putu Ayu Sisyawati Putriningsih, and I Putu Gede Yudhi Arjentinia Detection of Problems in Renal Dogs Using Ultrasound Method I Putu Gede Yudhi Arjentinia and Putu Ayu Sisyawati Putriningsih Seroprevalence Of Pig Cysticercosis In Endemic Area, Indonesia Ida Bagus Ngurah Swacita, I Ketut Suada and Ida Ayu Pasti Apsari The Index Of Bursa Fabrisius Post Vaccination Of Infectious Bursal Disease In Broiler Gusti Ayu Yuniati Kencana, I Nyoman Suartha, Dewa Ayu Paranitha, Ratih Komala Sari The Molecular Detection Of Citrus Vein Phloem Degeneration (Cvpd) Pathogen (Liberobacter Asiaticus) In Diaphorina Citri Kuwayama (Homoptera : Psyllidae) And Other Insects Associated With Citrus Plant I Nyoman Wijaya, Wayan Adiartayasa And Made Sritamin Virus Identification On Early Generation Seed Potatoes In Kembang Merta Village, Bali Putu Wina Andriani Lestari, Ida Ayu Astarini and Made Ria Defiani Weeds and Virus Identification of Potato Fields in Pancasari Village Deborah Margareth, Ida Ayu Astarini, Martin Joni, I Gede Rai Maya Temaja The Study Of The Citrus Vein Phloem Degeneration With Observe The Symptons Of Chlorosis And Using Primers Specific 16s Rdna On Citrus Plant In Karangasem Regency Wayan Adiartayasa, I N. Wijaya and I G. N. Bagus Plasmid Resistance Of Bradyrhizobium Japonicum From Agroforestry System To Pesticides Saidatul Idiyah, Hartawati, Amir Syarifudin, Laras Pratami, Ajeng Ludvita Rani and Rahayu Hapsari th ICBB 2017 xlv

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8 VIRUS IDENTIFICATION ON EARLY GENERATION SEED POTATOES IN KEMBANG MERTA VILLAGE, BALI Wina Andriani, Ida Ayu Astarini, Made Ria Defiani S2 Ilmu Biologi, Universitas Udayana

9 INTRODUCTION Potato production in Indonesia continue to decrease, due to use of late generation potato tubers as seeds Use of late generation has caused high disease infestation, particularly virus Need to use high quality, early generation seed potatoes Department of Agriculture, Bali and a number of farmers in Bedugul region, has started to use early generation seed potatoes. Need to confirm the quality of seed Research aim: To find out virus symptoms on early generation (G1) at Kembang Merta Village To confirm virus type using DAS-ELISA technique

10 Research Method Symptoms observation Horticulture Field Station, Kembang Merta Village, Tabanan, Bali Sample were taken from G1 potato field at 3 different screen house Fresh leaves with virus symptom were taken and keep on cool box and brought to lab

11 DAS ELISA METHOD Overnight Weigh each sample 4 grams & crushed until smooth Plate filled 100 μl antibody + wrapped using tissue & aluminum foil. Plate washed 3x with PBST The scoured liquid is inserted into the plate (100 μl) according to the elite & incubation map ± 2 hours

12 Enter each 100 μl conjugate at each sample & incubation well ± 2 hours Plate washed 8 x with PBST Plate washed 8 x with PBST Enter PNPP (100 μl) in each well Incubate room temperature minutes ELISA reader

13 RESULTS 1. Virus symptoms observed Mosaic Leaf Roll Malformasi

14 RESULTS 1. Virus symptoms observed Rugose Mottle

15 2. Serological test (DAS-ELISA) results Plants were virus positive: PVX, PVS, PVY and PLRV at all three potato screen house.

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17 A Positif PVX dan PVS B Positif PVX dan PVY C Positif PVX dan PVY

18 Conclusion and suggestion Conclusion : 1. Virus symptoms observed were: mosaic, leaf roll, rugose, malformation and mottle. 2. Early generation seed potato were infected with PVX (81,81 %), PVS (27,27 %), PVY (27,27 %) and, PLRV (18,75 %). Sugestion : 1. Improve screen house isolation and pest control should be done more intensively

19 Thanks You Staff employees at the Kembang Merta Village, Tabanan, Bali

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