GeneCopoeia provides five qpcr array formats (A, B, C, D, and E) suitable for use with the following realtime

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1 GeneCopoeia TM Expressway to Discovery ExProfile TM Human Myeloma Gene qpcr Array For focused group profiling of human myeloma related genes expression Cat. No. QG074-A (1 x 96-well plate, Format A) Cat. No. QG074-B (1 x 96-well plate, Format B) Cat. No. QG074-C (1 x 96-well plate, Format C) Cat. No. QG074-D (1 x 96-well plate, Format D) Cat. No. QG074-E (1 x 96-well plate, Format E) Available as 1 set or 6 sets. Each set contains 84 unique gene primers deposited in one 96-well plate. Introduction The ExProfile human myeloma gene qpcr array profiles 84 human genes to aberrantly expressed human genes involved in human myeloma. These genes are carefully chosen for their close cancer correlation based on a thorough literature search of peer-reviewed publications. Abnormal gene expression is often observed in cancer development and progression. The ExPofile human myeloma gene array allows researchers to study the cancer-related genes to gain understanding of their roles in myeloma pathogenesis. QG074 plate 01: 84 unique gene PCR primer pairs Shipping and storage condition Shipped at room temperate Stable for at least 6 months when stored at -20 C Array format GeneCopoeia provides five qpcr array formats (A, B, C, D, and E) suitable for use with the following realtime cyclers. Important note: Upon receiving, please check to make sure that the correct array format was ordered to ensure the compatibility with your qpcr instrument. Plate format Instrument provider qpcr instrument model A B C D E Applied Biosystems Applied Biosystems Bio-Rad Laboratories Bio-Rad Laboratories Roche Applied Science 5700, 7000, 7300, 7500, 7700, 7900HT (Standard 96-well block), ViiA TM 7 (Standard 96-well block) 7500 (Fast block), 7900HT (Fast block), StepOnePlus TM, ViiA TM 7(Fastblock) icycler iq,myiq,iq 5 CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 LightCycler 480 (96-well block) 1

2 Quality control 1. Each pair of primers in the ExProfile gene qpcr array has been experimentally validated to yield a single dissociation curve peak and to generate a single amplicon of the correct size for the targeted gene. 2. The positive PCR controls (PCR) have been verified to amplify a single amplicon of the correct size with Ct values around 20±2. 3. The Spike-in reverse transcription controls (RT) have been verified to amplify a single amplicon of the correct size with Ct values around 20±3. 4. R 2 > 0.99 was observed for high inter/ intra-array reproducibility. Materials required but not provided All-in-One TM First-Strand cdna Synthesis Kit All-in-One TM qpcr Mix Total RNA extraction kit (RNAzol RT RNA extraction reagent is recommended) DNase/RNase free tips, PCR reaction tubes, 1.5 ml microcentrifuge tubes 5 ml and 10 ml graduated pipettes, beakers, flasks, and cylinders 10 μl to1,000μl adjustable single channel micropipettes with disposable tips 5 μl to20μl adjustable multichannel micropipette, disposable tips, and reservoir qpcr instrument, compatible with gene qpcr arrays ordered Array layout Figure1. Illustration of QG074 plate 01 Gene primer pairs: 84 wells (A row to G row) are designated for a real-time PCR assay for genes (see the primer list). HK1-6: Six pre-deposited housekeeping gene (HK1-6) primer pairs, which can be used as endogenous positive controls as well as for array normalization. GDC: Genomic DNA controls, which can be used to specifically detect genomic DNA contamination with a high level of sensitivity. RT: Spike-in reverse transcription controls, which can be used to monitor the efficiency of the RT reactions. These pre-deposited primer pairs specifically amplify the cdna template reversed transcribed from the spike-in control RNA in the sample. PCR: Positive PCR controls, which are used to verify the PCR efficiency by amplifying the predeposited DNA template with its specific pre-deposited primer pairs. 2

3 Gene primer list Plate Position Catalog No. of Primer Accession No. of Gene Symbol QG A01 HQP NM_ IL6 QG A02 HQP NM_ TNF QG A03 HQP NM_ MTHFR QG A04 HQP NM_ ABCB1 QG A05 HQP NM_ IL1B QG A06 HQP NM_ TYMS QG A07 HQP NM_ NFKBIA QG A08 HQP NM_ NFKB1 QG A09 HQP NM_ IL10 QG A10 HQP NM_ IL6R QG A11 HQP NM_ CYP2C19 QG A12 HQP NM_ XRCC3 QG B01 HQP NM_ TP53 QG B02 HQP NM_ PON1 QG B03 HQP NM_ MTRR QG B04 HQP NM_ MTR QG B05 HQP NM_ IRF4 QG B06 HQP NM_ IL6ST QG B07 HQP NM_ IL4R QG B08 HQP NM_ IL4 QG B09 HQP NM_ IL1RN QG B10 HQP NM_ IL1A QG B11 HQP NM_ IGF1R QG B12 HQP NM_ FCGR2A QG C01 HQP NM_ EPHX1 QG C02 HQP NM_ CYP1A1 QG C03 HQP NM_ CTLA4 QG C04 HQP NM_ PPP1R13L QG C05 HQP NM_ NQO1 QG C06 HQP BC HLA-DRB1 QG C07 HQP NM_ NAT1 QG C08 HQP NM_ CD81 QG C09 HQP NM_ CD79B QG C10 HQP NM_ CD40 QG C11 HQP NM_ CD28 QG C12 HQP NM_ CD5 QG D01 HQP NM_ CD4 QG D02 HQP NM_ TNFSF18 QG D03 HQP NM_ CFLAR QG D04 HQP NM_ TNFRSF10A QG D05 HQP NM_ TNFRSF10B QG D06 HQP NM_ FADD QG D07 HQP NM_ TNFRSF14 3

4 4 QG D08 HQP NM_ TNFSF10 QG D09 HQP NM_ TRADD QG D10 HQP NM_ CASP3 QG D11 HQP NM_ TLR10 QG D12 HQP NM_ COL18A1 QG E01 HQP NM_ CASP8 QG E02 HQP NM_ XRCC5 QG E03 HQP NM_ XRCC4 QG E04 HQP NM_ VEGFA QG E05 HQP NM_ VCAM1 QG E06 HQP NM_ TRAF6 QG E07 HQP NM_ TRAF5 QG E08 HQP NM_ TRAF2 QG E09 HQP NM_ TNFRSF1B QG E10 HQP NM_ TLR1 QG E11 HQP NM_ RPS19 QG E12 HQP NM_ RELB QG F01 HQP NM_ RELA QG F02 HQP NM_ REL QG F03 HQP NM_ PTGS2 QG F04 HQP NM_ PSMA6 QG F05 HQP NM_ PPARG QG F06 HQP NM_ TNFRSF12A QG F07 HQP NM_ P2RX7 QG F08 HQP NM_ NFKBIE QG F09 HQP NM_ NFKB2 QG F10 HQP NM_ ABCC1 QG F11 HQP NM_ MPO QG F12 HQP NM_ LIG4 QG G01 HQP NM_ JAK3 QG G02 HQP NM_ IRS1 QG G03 HQP NM_ IL18 QG G04 HQP NM_ TNFRSF9 QG G05 HQP NM_ IL15 QG G06 HQP NM_ IL13 QG G07 HQP NM_ IL12RB2 QG G08 HQP NM_ IL12RB1 QG G09 HQP NM_ IL12B QG G10 HQP NM_ IL12A QG G11 HQP NM_ IL10RB QG G12 HQP NM_ IL8RB QG H01 HGDC QG H02 HGDC QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A

5 QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR 5

6 Limited Use License Following terms and conditions apply to use of ExProfile TM Myeloma Gene qpcr Array (the Product). If the terms and conditions are not acceptable, the Product in its entirety must be returned to GeneCopoeia within 5 calendar days. A limited End-User license is granted to the purchaser of the Product. The Product shall be used by the purchaser for internal research purposes only. The Product is expressly not designed, intended, or warranted for use in humans or for therapeutic or diagnostic use. The Product must not be resold, repackaged or modified for resale, or used to manufacture commercial products or deliver information obtained in service without prior written consent from GeneCopoeia. This Product should be used in accordance with the NIH guidelines developed for recombinant DNA and genetic research. Use of any part of the Product constitutes acceptance of the above terms. Limited Warranty GeneCopoeia warrants that the Product meets the specifications described in the accompanying Product Datasheet. If it is proven to the satisfaction of GeneCopoeia that the Product fails to meet these specifications, GeneCopoeia will replace the Product. In the event a replacement cannot be provided, GeneCopoeia will provide the purchaser with a refund. This limited warranty shall not extend to anyone other than the original purchaser of the Product. Notice of nonconforming products must be made to GeneCopoeia within 30 days of receipt of the Product. GeneCopoeia s liability is expressly limited to replacement of Product or a refund limited to the actual purchase price. GeneCopoeia s liability does not extend to any damages arising from use or improper use of the Product, or losses associated with the use of additional materials or reagents. This limited warranty is the sole and exclusive warranty. GeneCopoeia does not provide any other warranties of any kind, expressed or implied, including the merchantability or fitness of the Product for a particular purpose. GeneCopoeia is committed to providing our customers with high-quality products. If you should have any questions or concerns about any GeneCopoeia products, please contact us at GeneCopoeia, Inc. GeneCopoeia, Inc Medical Center Drive, Suite 101 Rockville, MD (301) (866) inquiry@genecopoeia.com GeneCopoeia Products are for Research Use Only Copyright 2016 GeneCopoeia, Inc. Trademarks: GeneCopoeia, ExProfile, All-in-One, (GeneCopoeia Inc.); RNAzol RT (Molecular Research Center, Inc.); SYBR (Molecular Probes); icycler iq, MyiQ, iq 5, CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 (Bio-Rad); LightCycler (Roche);Trizol, ABI,ROX, ViiA TM,StepOnePlus TM (Life Technologies). QG