Product Datasheet. GAPDH Antibody NB SS. Unit Size: ml. Store at 4C. Do not freeze. Reviews: 2 Publications: 13

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1 Product Datasheet GAPDH Antibody NB SS Unit Size: ml Store at 4C. Do not freeze. Reviews: 2 Publications: 13 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 8/29/2017 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB SS GAPDH Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity Buffer Target Molecular Weight ml 0.2 mg/ml Store at 4C. Do not freeze. Polyclonal 0.09% Sodium Azide IgG Immunogen affinity purified TBS and 0.1% BSA 36 kda Page 1 of 5 v.20.1 Updated 8/29/2017 Product Description Host Rabbit Gene ID 2597 Gene Symbol Species Reactivity Notes Marker GAPDH Human, Mouse, Rat, Primate Reacts with Human, Mouse, Rat and Primate sp. Based upon 100% sequence homology, this antibody is expected to react with Hamster (Chinese hamster, Golden Hamster), Canines, Ferret, Squirrel, Pig/Porcine, Bovine, Sheep, Rabbit, Xenopus and other amphibians such as Turtle, Avian species (including Chicken, Turkey, Sparrows, Duck), Zebrafish and several other fishes. Cytosolic Marker Immunogen The epitope recognized by this antibody maps to a region between residues 150 and 200 of human GAPDH using the numbering given in entry NP_ (GeneID 2597). Product Application Details Applications Recommended Dilutions Application Notes Western Blot, Simple Western, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin Western Blot 1:2000-1:10000, Simple Western 1:100, Immunohistochemistry 1:100-1:500, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:100-1:500 This GAPDH antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry-Paraffin applications. For IHC, antigen retrieval with citrate buffer ph6.0 is recommended for formalin fixed paraffin embedded tissue sections. In Simple Western only ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

3 Images Western Blot: GAPDH Antibody [NB ] - Detection of Human and Mouse GAPDH by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti- GAPDH antibody NB used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Page 2 of 5 v.20.1 Updated 8/29/2017 Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB ] - GAPDH detection in HeLa cells with ICC-IF application using NB , visualized with DyLight Fluor 488. Immunohistochemistry-Paraffin: GAPDH Antibody [NB ] - IHC-P detection of GAPDH in formalin fixed paraffin embedded section of mouse squamous cell carcinoma using NB at a dilution of 1:200. Western Blot: GAPDH Antibody [NB ] - Mouse DRG stained at 1:2000 dilution. Image provided by verified customer review.

4 Western Blot: GAPDH Antibody [NB ] - Detection of Human and Mouse GAPDH by Western Blot. Samples: Whole cell lysate from mouse NIH3T3 (5, 15 and 50 ug) and human HeLa (H; 50 ug) cells. Antibody: Affinity purified rabbit anti-gapdh antibody used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Page 3 of 5 v.20.1 Updated 8/29/2017 Immunohistochemistry-Paraffin: GAPDH Antibody [NB ] - IHC-P detection of GAPDH in formalin fixed paraffin embedded section of human lung carcinoma using NB at a dilution of 1:200. Simple Western: GAPDH Antibody [NB ] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the kda separation system.

5 Publications Rauen T, Frye BC, Wang J et al. Cold shock protein YB-1 is involved in hypoxia-dependent gene transcription. Biochem. Biophys. Res. Commun. Sep :00AM [PMID: ] Duprey-Diaz MV, Blagburn JM, Blanco RE. Optic nerve injury upregulates retinoic acid signaling in the adult frog visual system. J. Chem. Neuroanat. May :00AM [PMID: ] (WB) Wu JH, Liu JH, Ko YC et al. Haploinsufficiency of RCBTB1 is associated with Coats disease and familial exudative vitreoretinopathy Hum. Mol. Genet. Feb :00AM [PMID: ] (WB, Human) Nagy Z. Investigation Of Ras Oncogenic Signaling Modulators in the Formation and Progression of Cancer Thesis Nov 14 (WB, Human) Nagy Z, Kovacs I, Torok M et al. Function of RasGRP3 in the formation and progression of human breast cancer. Mol. Cancer Jun 02 [PMID: ] (WB, Human) Chevalier SA, Turpin J, Cachat A et al. Gem-Induced Cytoskeleton Remodeling Increases Cellular Migration of HTLV- 1-Infected Cells, Formation of Infected-to-Target T-Cell Conjugates and Viral Transmission. PLoS Pathog Mar 3 [PMID: ] (WB, Human) Lin L, McCroskery S, Ross JM et al. Induction of filopodia-like protrusions by transmembrane agrin: role of agrin glycosaminoglycan chains and Rho-family GTPases. Exp Cell Res 2010 Aug 15 [PMID: ] (WB, Primate) Harris JA, Koyama A, Maeda S et al. Human P301L-Mutant Tau Expression in Mouse Entorhinal-Hippocampal Network Causes Tau Aggregation and Presynaptic Pathology but No Cognitive Deficits PLoS One 2012 [PMID: ] (WB, Mouse) Huang Y, Bell LN, Okamura J et al. Phospho-deltaNp63alpha/SREBF1 protein interactions: Bridging cell metabolism and cisplatin chemoresistance Cell Cycle 2012 Sep 5 [PMID: ] (WB, Human) Thomas C, Zoubeidi A, Kuruma H et al. Transcription factor Stat5 knockdown enhances androgen receptor degradation and delays castration-resistant prostate cancer progression in vivo Mol Cancer Ther 2011 Feb [PMID: ] (WB, Human) Kim JI, Urban M, Young GD, Eto M. Reciprocal regulation controlling the expression of CPI-17, a specific inhibitor protein for the myosin light chain phosphatase in vascular smooth muscle cells Am J Physiol Cell Physiol 2012 Jul [PMID: ] (WB, Rat) Obexer P, Hagenbuchner J, Unterkircher T et al. Repression of BIRC5/Survivin by FOXO3/FKHRL1 Sensitizes Human Neuroblastoma Cells to DNA Damage-induced Apoptosis. Mol Biol Cell;20(7): [PMID: ] More publications at Page 4 of 5 v.20.1 Updated 8/29/2017

6 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: