Welcome at Your Automation Partner. Automation solutions for NGS in Forensic DNA research. Willem van Loon

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1 Welcome at Your Automation Partner Automation solutions for NGS in Forensic DNA research. Willem van Loon 1

2 Hamilton, your global partner FRANKLIN RENO BONADUZ 2

3 Hamilton Business Units ROBOTICS STORAGE LAB & SENSORS MEDICAL 3

4 Forensics Solutions Sample preparation

5 Our focus on Forensic Automation A broad range of Solutions for every Forensics needs based on a worldwide experience 5

6 New solution for fully Sample Preparation automated lysis Hamilton AutoLys STAR 6

7 AutoLys Tube workflow Insert sample Add Lysis Buffer Incubate & Shake Lift & Lock Centrifuge Clear Lysate in outer tube Remove tube & proceed with DNA purification S pi n 7

8 AutoLys Key Features AutoLys heater shaker. Designed for optimal heat transfer and shaking efficiency EasyCode 2D barcode reader. For total traceability throughout the lysis process Integrated ondeck centrifuge formaximum lysate recovery for maximum DNA yields AutoLys channels and tubes for a fully automated and uninterupted preparation of clear lysates 8

9 Forensic Workflow solutions Sample Sample Lysys Preparation DNA Purification Quantification setup Normalization & STR setup CE setup ID STARlet Workstation 9

10 Hamilton easypunch Complete automation of dried samples on paper cards Automated card handling Integrated imaging for sample analysis Automated punching and plate positioning And liquid handling 10

11 Next Generation Forensics Solutions

12 Illumina s Suite of Library Prep Solutions TruSeq RNA TruSeq Stranded mrna TruSeq Stranded Total RNA TruSeq Small RNA TruSeq Targeted RNA Expression TruSeq RNA Access TruSeq ChIP TruSeq DNA PCR-Free TruSeq Nano DNA Nextera Nextera XT Nextera Mate Pair TruSeq Synthetic Long Read TruSeq Rapid Exome TruSeq Custom Amplicon TruSight Tumor TruSeq Amplicon Cancer Panel TruSight Myeloid Nextera Rapid Capture Exome/Custom TruSight Panels 12

13 Hamilton Offerings for Illumina Library Prep Hamilton Microlab STAR 3 Configurations NGS STAR for HiSeqX / SeqLab NGS STAR for VeriSeq NGS STAR for TruSeq Illumina Qualified Methods 13

14 Illumina Qualified Methods Partnership Details Automated Methods System Support Availability TruSeq mrna STAR Total RNA STAR RNA Access STAR TruSeq Nano TruSeq Amplicon STAR STAR STARlet Hamilton First Line Support Available now TruSeq PCR-Free STAR Nextera XT TruSeq Rapid Exome STAR STARlet STAR Coming Soon +20 additional apps 14

15 What about NGS in Forensics?? About 50 Next Gen Sequencers installed in EU labs Pioneers Forensic Research labs (universities) 15

16 What about NGS in Forensics?? What is the opinion on NGS?: Recent meetings: ENFSI: 20% of discussions on NGS Other: 60% of discussions on NGS During breaks, NGS is main topic Conclusions: - NGS is a great tool for degraded samples and mixes - NGS won t replace STR within 5-6 years - Law needs to change to accept NGS results 16

17 What about NGS in Forensics?? Where does that take us as Automation Company? - NGS is here to stay - Need for automation - Cooperation is essential - Project started with INPS Lyon, Laurent Pene - Goal: to obtain a dedicated system for ForenSeq library preparation 17

18 Workflow overview Illumina kits 18

19 No matter the input, all libraries end up looking similar Dual Index Library shown The aim of the Library Prep step is to obtain nucleic acid fragments with adapters attached on both ends 19

20 ForenSeq DNA Signature Prep WorkFlow 20

21 Application discussed Instrument? LIMS? Decklayout? Labware? Shaker? User management? Magnet? Heater? 21

22 Translation to robotic workflow finished 22

23 Method development started 23

24 Time frame to validated method Method programming Started Implementation 2 nd week of June Optimization July Validation September (August holidays) 24

25 Thank you for your attention 25