Ch.15 Section 4 Regulation of Gene Expression pgs Complete the attached Active Reading Guides for the above sections.

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1 AP Biology Summer Assignment Due Wednesday 9/5/2018 Text Book Reading Ch.13 The Molecular Basis of Life pgs Ch.15 Section 4 Regulation of Gene Expression pgs Active Reading Guides Complete the attached Active Reading Guides for the above sections. Note: All work is to be hand written. No Exceptions. Directions: For Each Chapter: Read each chapter and study the vocabulary as you move through the chapter. At this level of Biology a strong command of the vocabulary is essential. This means that not only should you be able to define a term but you should understand that term in relation to the concept that is supports. Have a Great Summer! We are looking forward to a great year together. Mrs. O Rourke and Mrs. Trozzo Jennifer.Orourke@rtsd.org Karen.Trozzo@rtsd.org

2 Name: AP Biology Mr. Croft Chapters 13.4 & 15.4 Active Reading Guide Biotechnology The AP Biology exam has reached into this chapter for essay questions on a regular basis over the past 15 years. Student responses show that biotechnology is a difficult topic. This chapter requires a strong conceptual understanding of the technological processes and the underlying biology that guides the procedure. With a little careful work, this chapter will give you insights into the incredible advancements already made and a basis for understanding the new marvels yet to be discovered in biotechnology. 1. It is important to understand the meaning of the three terms in bold to start this chapter. recombinant DNA: biotechnology: genetic engineering: 2. Plasmids are important in biotechnology. Give a full and complete definition of plasmid. 3. The production of multiple copies of a single gene is called. 4. Using Figure in your text, explain the four steps in this preview of gene cloning. 3) 4) 1

3 5. Read the description of restriction enzymes in your text carefully. Then draw and explain each step of Figure What is a cloning vector? Why are bacterial plasmids widely used as cloning vectors? 7. Study the following figure for an example of a gene cloning experiment. Explain the following key points. a. What is the source of the gene of interest? b. Explain why the plasmid is engineered with amp R and lacz. 2

4 c. Why are both the gene of interest and the plasmid cut with the same restriction enzyme? d. What is the role of DNA ligase in this process? e. After transformation has occurred, why are some colonies blue? f. Why are some colonies white? Why is this important? 8. Once the hummingbird DNA is cloned, we have the problem of finding the piece of DNA that holds our gene of interest. Explain how nucleic acid hybridization will accomplish this task. 9. Getting a cloned eukaryotic gene to function in bacterial host cells can be difficult. What are two problems with bacterial gene expression systems, and how is each solved? 10. The polymerase chain reaction (PCR) is a Nobel Prize winning idea that is used by scientists to amplify DNA, particularly when the quantity of DNA is very small or contaminated. Explain the three initial steps that occur in cycle 1 of PCR. 3) 3

5 11. How many molecules will be produced by four PCR cycles? 12. is a technique used to separate nucleic acids or proteins that differ in size or electrical charge. 13. Why is the DNA sample to be separated by gel electrophoresis always loaded at the cathode or negative end of the power source? 14. Explain why shorter DNA molecules travel farther down the gel than larger molecules. 15. A patient who is a carrier for sickle-cell anemia would have a gel electrophoresis pattern showing four bands. The carrier is heterozygous and therefore has both alleles. The normal allele will produce three fragments. The abnormal allele will produce two fragments. Explain why the gel shows a four-band pattern. 16. How is RT-PCR used? 17. What are DNA microarray assays? 18. Why are the four nucleotides in DNA each labeled with a different color of fluorescent tag? 4

6 19. Explain the four steps of DNA microarray assays. 3) 4) 20. Explain how microarrays are used in understanding patterns of gene expression in normal and cancerous tissue. 5