(Original Article) Silibinin. Multipotent. Silibinin DMEM/F12. Bulge. ( III Tubulin) P< Bulge. Silibinin (NT3) Silibinin. Silibinin.

Transcription

1 (Original Article Multipotent Silybummarininum. EGF Vibrissa DMEM/F12 Neurotrophin-3 ( ( manob@iums.ac.ir

2 DNA ( HL60 Neural Crest Differentitation.( In ( (Pluripotent Vitro In Vitro Wistar Milk Thistle Silybum Marianum Kobayashi Amoh B A X II DMEM/F12 AST ALT un/ml (

3 B Normal Goat Serum EDTA (Ethylenediamine Tetracetic Acide rpm FITC DAPI Cover Slip DMEM/F12 (3/1 (Epidermal Growth Factor EGF 10mg/ml FBS (5% Aldrich- Sigma M (Sigma-Aldrich Mml In Vitro CO2 EGF EGF Neurotrophin FITC CO2 Positive (ph=7.4 FITC ( Normal Goat Serum :

4 FITC. : EGF FITC 1 g/ml

5 .( m/ml NT 3 m/ml

6 . DAPI 0.5 µg/ml Intermediate Filament Protein PNS CNS III III DAPI 0.5µg/ml Tubulin GFAP Tubulin

7 .( Smita Tubulin Tubulin beta alpha beta Differentiation beta IV beta (II HL60 beta III.(. In Vitro (

8 Toxin Proliferation Neurotrophicfactor (Proliferation Beta III References: 1. Alonso L, Fuches E. Stem Cells in the Skin:Waste Not. Wnt Not Genes & Development 2003;17: Sieber-Blum M, Grim M, Hu F, Szeder V. Pluripotent Neural Crest Stem Cells in the Adult Hair Follicle. Dev Dyna 2004;231: Amoh Y, Li L, Katsuoka K, Penman S, Robert M. Multipotent -Positive, Keratin-Negative Hair-Follicle Stem Cell Can Form Neurons. PNAS 2005;102: Zhang Y, Xiang M, Wang Y, Yan J, Zeng Y. Cells Human Hair Follicles Segregation Cultivation and Properties. Coll & Surfaces 2006;47: Hong Yu, Dong F, Suresh M, Ling Li. Isolation of a Novel Population of Multipotent Adult Stem Cells from Human Hair Follicles. Am J Pathol 2006;168: Li L, Mignone J, Yang M, Matic M, Penman S. Expression in Hair Follicle Sheath Progenitor Cells. PANS 2003;100(17: Der Marderosin A. The Review of Natural Product. Missouri. Fact and Comparison 2001; : Fredstorn S. Nitric Oxide, Oxidative Stress and Dietary Antioxidant. Nutrition 2002;18: Cayuela M. Oxygen Free Radicals and Human Disease. Bichemistery 1995;779-Chang. 10. Carin R, Comoglio A, Albano E, Poli G. Lipid Peroxidation and Irreversible Damage in the Rat Hepatocyte Model Protection by the -Phospholipids Complex IdB Biochem Pharmacol 1992;43:

9 11. Smita K, Skuntala W, Ward A. Neurotrophic and Neuroprotective Effect of Milk Thistle (Silybum marianum on Neurons in Culture. J Mol Neurosci 2002;18: Kobayashi K, Rochat A, Barrandon Y. Segregation of keratinocyte Colony-Forming Cells in the of the Ratvibrissa. PNAS 1993;90: Alcmeme C, Tuan D, Taube P. Neurotrophin-3 is Required for the Survival- Differentation Subsets of Developing Enteric Neurons. The Journal of Neuroscience 2001;21(15: Schulz V, Hansel R, Tyler VE. Rational Phytotherapy: A Physicians Guide to Herbal Medicine. Berlin: Springer;1997. p Kang SN, Lee MH, Kim KM, Cho D, Kim TS. Induction of Human Promyelocytic Leukemia HL-60 Cell Differentiation into Monocytes by : Involvement of Protein Kinase C. Biochem Pharmacol 2001; Raff E, Fackenthal J. Microtubule Architecture Specified by a Beta Tubulin Isoform. Science 1997;

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