Product Datasheet. LHR Antibody NLS1436. Unit Size: 0.05 ml. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

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1 Product Datasheet LHR Antibody NLS1436 Unit Size: 0.05 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 1 Publications: 4 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 10/31/2017 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NLS1436 LHR Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity Buffer 0.05 ml 1.0 mg/ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Polyclonal 0.1% Sodium Azide IgG Immunogen affinity purified PBS Page 1 of 5 v.20.1 Updated 10/31/2017 Product Description Host Rabbit Gene ID 3973 Gene Symbol Species Reactivity Notes Specificity/Sensitivity Immunogen LHCGR Human Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (95%), Panda (95%), Bovine (89%), Bat (89%), Rabbit (89%), Equine (89%), Ferret (84%). Human hcg receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins. Synthetic 19 amino acid peptide from C-terminus of human hcg receptor. Product Application Details Applications Recommended Dilutions Immunohistochemistry-Paraffin Immunohistochemistry-Paraffin 5 ug/ml

3 Images Immunohistochemistry-Paraffin: LHR Antibody [NLS1436] - IHC of human Breast, Carcinoma. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Page 2 of 5 v.20.1 Updated 10/31/2017 Immunohistochemistry-Paraffin: LHR Antibody [NLS1436] - Analysis of anti-lhr receptor antibody with the middle size antral follicle (1.5 mm) of human ovary. Image from confirmed customer review. Immunohistochemistry-Paraffin: LHR Antibody [NLS1436] - Analysis of anti-lhr receptor antibody with human ovary, corpus luteum at 5 ug/ml. Immunohistochemistry-Paraffin: LHR Antibody [NLS1436] - Human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffinembedded tissue after heat-induced antigen retrieval.

4 Publications Page 3 of 5 v.20.1 Updated 10/31/2017 Scarlet D, Aurich C, Ille N et al. Anti-Muellerian Hormone, inhibin A, gonadotropins and gonadotropin receptors in bull calves after partial scrotal resection, orchidectomy and Burdizzo castration. Theriogenology Jul :00AM [PMID: ] (IHC) Noci I, Sorbi F, Mannini L et al. LH/hCG-Receptor Expression May Have a Negative Prognostic Value in Low-Risk Endometrial Cancer. Front Oncol Sep :00AM [PMID: ] Yung Y, Aviel-Ronen S, Maman E et al. Localization of Luteinizing Hormone Receptor Protein in the Human Ovary. Mol. Hum. Reprod May 29 [PMID: ] (IHC-P, Human) Martin I, Rodrigues MMP, Fujihara CJ et al. Localization patterns of steroid and luteinizing hormone receptors in the corpus luteum of Nelore (Bos taurus indicus) cows throughout the estrous cycle. Livestock Science Aug (IHC, Bovine)

5 Procedures Immunohistochemistry Protocol for Luteinizing Hormone Receptor Antibody (NLS1436) Immunohistochemistry 1. Prepare tissue with formalin fixation and by embedding it in paraffin wax. 2. Make 4 um sections and place on pre-cleaned and charged microscope slides. 3. Heat in a tissue-drying oven for degrees Celcius. 4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes RT. 5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes RT. 6. Wash the slides in 2 changes of 95% alcohol for 3 minutes RT. 7. Wash the slides in 1 change of 80% alcohol for 3 RT. 8. Rinse the slides in gentle running distilled water for 5 RT. 9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (ph degrees Celcius for 20 minutes. 10. Remove the slides from the heat and let stand in RT for 20 minutes. 11. Rinse the slides in 1X TBS-T for 1 RT. **Do not allow the tissues to dry at any time during the staining procedure** Page 4 of 5 v.20.1 Updated 10/31/ Begin the immunostaining by applying a universal protein block for 20 RT. 13. Drain protein block from the slides and apply the diluted primary antibody for 45 RT. 14. Rinse the slide in 1X TBS-T for 1 RT. 15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 RT. 16. Rinse the slide in 1X TBS-T for 1 RT. 17. Apply an alkaline phosphatase steptavidin for 30 RT. 18. Rinse the slide in 1X TBS-T for 1 RT. 19. Apply an alkaline phosphatase chromagen substrate for 30 RT. 20. Rinse the slide in distilled water for 1 RT. **This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)** 21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute RT. 22. Wash the slides in 2 changes of 95% alcohol for 1 minute RT. 23. Wash the slides in 3 changes of 100% alcohol for 1 minute RT. 24. Wash the slides in 3 changes of xylene for 1 minute RT. 25. Apply cover slip.

6 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NLS1436 HAF008 NB7156 NBP G1-200 Goat anti-rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat anti-rabbit IgG (H+L) Secondary Antibody Rabbit IgG Isotype Control IGF-I [Unconjugated] Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: