Bio 205 & Bio 298AA Fall 2015 Laboratory Policies and Schedule

Transcription

1 Bio 205 & Bio 298AA Fall 2015 Laboratory Policies and Schedule It is your responsibility to be familiar with the policies given in this syllabus. Prof. Anne Healy Office phone: Office: NS 118 Office hours: M/W T/Th Fri 10:30 a.m. 11:45 a.m. or after lab 8:30 a.m. 8:45 a.m. or after lab By appointment. Grading: 6 lab 10 pts each 60 (8 taken, lowest 2 dropped) Lab Midterm Exam 100 Lab Final Exam 100 Total points 260 Attendance: After 2 consecutive lab absences you will be withdrawn. After 3 total non-consecutive absences you will be withdrawn. Lab Quizzes: See the lab schedule for quiz dates. Missed lab quizzes cannot be made up, but your lowest 2 lab quiz grades will be dropped. Missed lab work and lab exercises cannot be made up. Lab drawers: There are plenty of drawers that you may use to store goggles, old sneakers, or hair clips between lab periods. Week of: 8/24 Lab 1 Lab exercises (This schedule may be modified in the event of instructor illness. Students will be informed of changes.) Overview of lab rules Introduction to the Light Microscope Effectiveness of hand washing

2 8/31 Lab 2 Lab quiz 1 Common Aseptic Transfers and Inoculation Methods Ubiquity of Microorganisms Simple Stains 9/7 No labs due to Labor Day holiday. 9/14 Lab 3 9/21 Lab 4 9/28 Lab 5 10/5 Lab 6 10/12 Lab 7 10/19 Lab 8 Lab quiz 2 Gram Stain Acid-fast Stain Lab quiz 3 Capsule Stain Endospore Stain Lab quiz 4 PCR Yeast ID using MUGAL & PRO Lab Midterm Exam over Labs 1-5. Gel Electrophoresis Phenylethyl Alcohol Agar Mannitol Salt Agar Snyder Agar Catalase Test (Inoculate Nutrient agar) Lab quiz 5 StaphAurex Test using growth from last week s MSA plate IdentiCult AE-PYR (E. faecalis) Catalase Test follow-up Inoculate: OF media MRVP broths Citrate slants Oxidase test (Inoculate Nutrient agar) Nitrate broths EMB agar

3 10/26 Lab 9 11/2 Lab 10 Lab quiz 6 over StaphAurex, PYR, Catalase, OF media Follow-up tests on: MRVP broths Nitrate broths Oxidase test on BBL Dry Slide Lab quiz 7 over MRVP, Citrate, Oxidase, Nitrate, EMB agar Anaerobic Jar Blood & Chocolate Agars Bacterial Transformation 11/11 No labs due to Veterans Day holiday 11/16 Lab quiz 8 Antiseptics & Disinfectants (disk diffusion) Antimicrobial Susceptibility Test (Kirby-Bauer Method) Germicidal effect of UV light 11/23 No labs due to Thanksgiving holiday 11/30 Lab 12 ELISA 12/7 Lab Final Exam over Labs 6-12.

4 Microbiology Lab Rules These apply to all students taking Bio 205 or Bio 298AA. Failure to observe rules will result in disciplinary action. 1. Do NOT eat, drink, chew gum, or apply lip balm or cosmetics. 2. Store all nonessentials (books, purses, phones) on the shelves or in the drawers. 3. Wipe down lab work area with Amphyl disinfectant. 4. Wash hands with Hibiclens. 5. Do NOT remove microbes or lab equipment from lab. 6. Wear goggles, closed-toe shoes, lab coat (or old clothing), gloves, and tie back long hair. 7. If you are feeling too sick to come to lab, leave me an or voice mail message or tell me after lecture. 8. If you are pregnant, immunocompromised, scheduled for surgery, or taking immunosuppressive drugs, it may be in your long-term best interest to take micro another semester. Please see your health care provider for advice. 9. Cover scratches on hands with a bandage (we have some) and then a glove. 10. Never pipette by mouth. 11. Dispose of used slides and broken glass in the white plastic sharps box that I will point out. 12. If you spill something, please notify me immediately. Same if a part falls off your microscope. 13. Keep test tubes upright in racks. Keep cultures covered if you are not using them. 14. Contaminated test tubes: remove tape and place in racks on discard counter. 15. Contaminated Petri dishes, swabs, or gloves: toss in autoclave bag.

5 Lab Syllabus Acknowledgement I am aware that there are risks involved in performing microbiology lab procedures and that I will be working with potentially hazardous chemicals and living microorganisms. If I suspect I am at increased risk of infection, I agree to notify my health care provider that I am taking microbiology lab and my instructor so accommodations can be made, if possible. I have read the lab safety information and rules and comprehend their importance. I will employ proper techniques at all times while working in the lab in order to minimize the dangers. If a procedure is unclear, I will ask my instructor for clarification. Student name (print): Student signature: Date: