L - Laboratory 5 A - Lecture 1 Total Hours per Week : 6 Total Number of Weeks : 13 LABORATORY COURSE IN MICROBIOL LABORATORY COURSE IN MICROBIOLOGY

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1 Page 1 of 3 10/24/2013 New Course Program Affected? Program Change Form Submitted? New Data Y Subject/Course/Term MIMM 384 N (Simple Change) - Course is being added to: 1. Honours Immunology (Interdepartmental) (75 credits) - U2 Complementary Courses 2. Honours Microbiology and Immunology (74 credits) - U2 Required Courses (21 credits) 3. Liberal Program - Core Science Component Microbiology and Immunology (49 credits) - U2 Required Courses (15 credits) 4. Major Microbiology and Immunology (68 credits) - U2 Required Courses (21 credits) Proposal Reference : 7307 Number PRN Alias : 13-14#720 Version No : 6 Submitted By : Dr Gregory T Marczynski Edited By : Ms Josie D'Amico Display Printable PDF Credit Weight or CEU's Course Activities Course Title Rationale Responsible Instructor Course Description 3 credits one term Schedule Type Hours per week L - Laboratory 5 A - Lecture 1 Total Hours per Week : 6 Total Number of Weeks : 13 Official Course Title : Course Title in Calendar : LABORATORY COURSE IN MICROBIOL LABORATORY COURSE IN MICROBIOLOGY We are splitting a cumbersome 2 semester 6 credit course MIMM 386D1/D2 into 2 3 credit courses, MIMM 384 and MIMM 385. This division will create courses that more closely match the microbiology (MIMM 384) and the immunology (MIMM 385) lab courses of other universities. Therefore this change will better accommodate one semester exchange students. As well this change will allow us to better administer, teach and modernize these topics. Benoit Cousineau A series of laboratory exercises in molecular biology and microbial genetics during the Fall term. As part of a term paper writing project, students will explore contemporary research subjects that represent the wide interests of the Microbiology and Immunology department s faculty. The general objective is to provide practical and theoretical introduction to the technology and methodology used in contemporary microbiology research.

2 Page 2 of 3 10/24/2013 Teaching Dept. Administering Faculty/Unit 0232 : Microbiology & Immunology SC : Faculty of Science Prerequisites MIMM 211, MIMM 212 Web Registration Blocked? : N Corequisites MIMM 314, MIMM 323, MIMM 324 Web Registration Blocked? : Y Restrictions Supplementary Calendar Info Additional Course Charges Campus Downtown Projected Enrollment 120 Requires Resources N Not Currently Available Explanation for Required Resources Required Text/Resources Sent To Library? Library Consulted About Availability of Resources? Consultation Reports Attached? Effective Term of Implementation Not open to students who are taking or have taken MIMM 386D1/D File Attachments Course Outline MIMM384.pdf View To be completed by the Faculty For Continuing Studies Use Approvals Summary Show all comments Version No. Departmental Curriculum Committee Departmental Meeting Departmental Chair Other Faculty Curric/Academic Committee Faculty SCTP Version Status 6 Submitted to

3 Page 3 of 3 10/24/2013 Edited by: Josie D'Amico on: Oct Submitted to Edited by: Gregory T Marczynski on: Oct Submitted to Edited by: Gregory T Marczynski on: Oct Submitted to Edited by: Gregory T Marczynski on: Oct Submitted to Edited by: Malek Yalaoui on: Oct Submitted to Created on: Oct

4 LABORATORY COURSE IN MICROBIOLOGY MIMM 384 (This is the current outline for MIMM 386D1, Fall 2013) COURSE COORDINATOR Prof. Benoit Cousineau Lyman Duff Medical Building, Room 617, Course Description This course presents the student with a series of laboratory exercises in molecular biology and microbial genetics during the Fall term. Also, as part of a term paper writing project, students will also explore contemporary research subjects in the fields of bacteriology, virology, mycology, parasitology and immunology that represent the wide interests of the Microbiology and Immunology department s faculty. The general objective is to provide practical and theoretical introduction to the technology and methodology used in contemporary microbiology research. LEARNING OBJECTIVES By the end of this course, students will be able to: -- Follow an experimental protocol. -- Perform laboratory techniques commonly used in microbiology. -- Design experiments with appropriate standards and controls. -- Analyze properly microbiology research data. -- Present research results in a clear and efficient manner. -- Use a scientific approach to problem solving. -- Search efficiently the scientific literature. -- Analyze, interpret, summarize and clearly present scientific information. PREREQUISITES: MIMM Biology of Microorganisms MIMM Laboratory in Microbiology COREQUISITES: MIMM Immunology MIMM Microbial Physiology and Genetics MIMM Fundamental Virology COURSE CONTENT Standard techniques for handling reagents, solutions and cultures Protein quantitation assays Endonuclease restriction analysis Plasmid preparation

5 Bacterial / E. coli transformation PCR analysis Agarose gel electrophoresis analysis PAGE gel electrophoresis analysis GST fusion protein purification Protein Immuno-detection Medical Bacteriology testing Bacterial (Staphylococci and Streptococci) isolation and identification Bacterial / Caulobacter isolation by enrichment cultures Bacterial / Caulobacter transposon mutagenesis Bacterial / Caulobacter temperature-sensitive mutation screening Bacterial / Caulobacter plasmid conjugation and mobilization Bacterial genetic complementation Transcriptional lacz reporter analysis INSTRUCTIONAL APPROACHES 1. Consecutive sections of lectures and laboratory exercises 2. Concurrent Term Paper Project Writing of a term paper on a topic in the general area of bacteriology, virology, mycology, parasitology or immunology. Each student will be assigned a topic by a professor from the Department of Microbiology and Immunology who will supervise the writing of the term paper. TIMETABLE Mondays, 2:30-- 3:30 pm: Pre-Laboratory Lectures Lyman Duff Medical Building Amphitheatre. Wednesdays, 1:30-- 5:30 pm: Laboratory exercises Laboratory Cubicles, 4 th floor of the Lyman Duff Medical Building. Fridays, 1:30-- 2:30 pm: Experimental laboratory follow-up periods (as required) Laboratory Cubicles, 4 th floor of the Lyman Duff Medical Building Fridays, 2:30-- 3:30 pm: Lectures (as required) Lyman Duff Medical Building Amphitheatre. COURSE CO-COORDINATORS Molecular biology: Prof. Benoit Cousineau Lyman Duff Medical Building, Room 617, benoit.cousineau@mcgill.ca Microbial genetics: Prof. Greg Marczynski Lyman Duff Medical Building, Room 506, gregory.marczynski@mcgill.ca Term paper project: Prof. Dalius Briedis Lyman Duff Medical Building, Room 502, dalius.briedis@mcgill.ca

6 TEACHING ASSISTANTS: 4 to be named LABORATORY STAFF: Aghdas Zamani, aghdas.zamani@mcgill.ca Richard Arthur, richard.arthur@mcgill.ca Shan Hong Zhang, shanhong.zhang@mcgill.ca COURSE CALENDAR (Fall 2013) LECTURERS: D. Briedis (DB), B. Cousineau (BC), S. Fournier (SF), A. Koromilas (AK), G. Marczynski (GM) ROOMS: Timmins (MNI) = Jeanne Timmins Amphitheater Montreal Neurological Institute (MNI) Amphitheatre= Lyman Duff Medical Building Amphitheatre. Cubicles = Laboratory Cubicles, 4 th floor of the Lyman Duff Medical Building DAY/ DATE Wed/ 4 Sept. Fri/ 6 Sept. Mon/ 9 Sept. Wed/11 Sept. Fri/ 13 Sept. Mon/ 16 Sept. Wed/ 18 Sept. Fri/ 20 Sept. Mon/ 23 Sept. TIME / ROOM / ACTIVITY: 1:30 p.m. / Timmins (MNI) / Lecture: Introduction to MIMM 386 (BC and SF) 2:30 p.m. / Amphitheatre / Lecture: The Term Paper project (DB) 3:30 p.m. / Meeting with term paper supervisors 2:30 p.m. / Amphitheatre / Lecture: Protein assays (BC) 1:30 p.m. / Cubicles / Laboratory: Protein assays (BC) 2:30 p.m. / Amphitheatre / Lecture: On-line search of the scientific literature (DB) 2:30 p.m. / Amphitheatre / Lecture: Endonuclease analysis (BC) 1:30 p.m. / Cubicles / Laboratory: Endonuclease restriction analysis of plasmid preparations (BC) 2:30 p.m. / Amphitheatre / Lecture: Term paper organization (DB) 2:30 p.m. / Amphitheatre / Lecture: PCR analysis (BC)

7 Wed/ 25 Sept. Mon/ 30 Sept. Wed/ 2 Oct. Fri/ 4 Oct. Mon/ 7 Oct. Wed/ 9 Oct. Fri/ 11 Oct. Wed/ 16 Oct. Fri/ 18 Oct. Mon/ 21 Oct. Wed/ 23 Oct. Fri/ 25 Oct. Mon/ 28 Oct. Wed/ 30 Oct. Fri/ 1 Nov. Mon/ 4 Nov. Wed/ 6 Nov. Fri/ 8 Nov. Mon/ 11 Nov. 1:30 p.m. / Cubicles / Laboratory: PCR to determine gene orientation in plasmids (BC) 2:30 p.m. / Amphitheatre / Lecture: Bacterial transformation (BC) 1:30 p.m. / Cubicles / Laboratory: Transformation of E. coli with a plasmid (BC) 1:30 p.m. / Cubicles / Lab: Cast PAGE gels for use on Wed (AK) 2:00 p.m. / Cubicles / Lab: Count transformed colonies (BC) 2:30 p.m. / Amphitheatre / Lecture: Purification, electrophoresis and transfer of GST fusion protein (AK) 1:30 p.m. / Cubicles / Laboratory: Purification, electrophoresis and transfer of GST fusion protein (AK) 2:30 p.m. / Amphitheatre / Lecture: Immuno-detection (AK) 1:30 p.m. / Cubicles / Laboratory: Immuno-detection of GST fusion (AK) Meeting with your term paper supervisor. Hand in term paper outline (10% of grade). Meetings scheduled by the students and supervisors. 2:30 p.m. Amphitheatre / Lecture: Medical Bacteriology (GM) 1:30 p.m. / Cubicles / Laboratory: Staph. & Strep. (GM) 1:30 p.m. / Cubicles / Follow-up: Staph. & Strep. (GM) 2:30 p.m. Amphitheatre / Lecture: Medical Bacteriology (GM) 1:30 p.m. / Cubicles / Laboratory: Staph. & Strep. (GM) 1:30 p.m. / Cubicles / Follow-up: Staph. & Strep. (GM) 2:30 p.m. / Amphitheatre / Lecture: Bacterial Genetics (GM) 1:30 p.m. / Cubicles / Laboratory: Bacterial Genetics, Caulobacters (GM) 1:30 p.m. /Timmins (MNI)/ Midterm exam Molecular biology section (labs 1-6) 2:30 p.m. / Amphitheatre/ Lecture: Bacterial Genetics, Caulobacters (GM)

8 Wed/ 13 Nov. Fri/ 15 Nov. Mon/ 18 Nov. Wed/ 20 Nov. Fri/ 22 Nov. Mon/ 25 Nov. Wed/ 27 Nov. Fri/ 29 Nov. 1:30 p.m. / Cubicles / Laboratory: Bacterial Genetics, Caulobacters (GM) 1:30 p.m. / Cubicles / Follow-up: Bacterial Genetics, Caulobacters (GM) Meeting with term paper supervisors to review first draft of term paper. 2:30 p.m. / Amphitheatre/ Lecture: Bacterial Genetics, Caulobacters (GM) 1:30 p.m. / Cubicles / Laboratory: Bacterial Genetics, Caulobacters (GM) 1:30 p.m. / Cubicles / Follow-up: Bacterial Genetics, Caulobacters (GM) 2:30 p.m. / Amphitheatre/ Lecture: Bacterial Genetics, Caulobacters (GM) 1:30 p.m. / Cubicles / Laboratory: Bacterial Genetics, Caulobacters (GM) 1:30 p.m. / Cubicles / Follow-up: Bacterial Genetics, Caulobacters (GM) Mon/ 2 Dec. 2:30 p.m. / Cubicles / Wrap-up: Bacterial Genetics (GM) COURSE MATERIAL Required Texts: Laboratory manual: Molecular biology and Microbial Genetics (Manuals can be purchased in the Office of the Microbiology and Immunology Department) COURSE EVALUATION -- Laboratory Reports: A 3-page report per laboratory session that includes purpose, introduction, result and discussion sections. Laboratory reports are due 1 week after the completion of the lab exercises. -- Laboratory exercise and are jointly submitted by the laboratory partners. Pre-Lab Quizzes: 5 short-answer type questions at the beginning of each laboratory exercise. -- Exams: Midterm (Molecular biology) and Final (Microbial genetics), each is a two-hour written examination. -- Term paper: A 10-page review of original papers in the scientific literature on an assigned topic. Laboratory reports 25% Pre-lab quizzes 5% Exams (midterm and final) 45% Term paper 25% 100%

9 Important notes on grading: -- 5% deduction will be applied for each day of late submission of the laboratory reports and term paper. The mark for late submissions beyond a week is zero. -- A documented excuse (e.g. Doctor s note) for missing a lab session or an exam should be submitted to the course coordinator. The mark of the other exam will then count for 25% of the final course grade. The mark for a missed lab session or exam without a documented excuse is zero. Note regarding missed laboratory exercise: As the material in all the laboratory exercises will be covered on the exams, it is strongly recommended that you obtain the data for any missed exercise from your laboratory partner and that you also contribute to the writing of the laboratory report. This will ensure that you understand the scientific principles, technical details and logical analysis of this exercise for the exams. MCGILL UNIVERSITY POLICY STATEMENTS "McGill University values academic integrity. Therefore, all students must understand the meaning and consequences of cheating, plagiarism and other academic offences under the Code of Student Conduct and Disciplinary Procedures. For more information, see Approved by Senate on 29 January "L'université McGill attache une haute importance à l honnêteté académique. Il incombe par conséquent à tous les étudiants de comprendre ce que l'on entend par tricherie, plagiat et autres infractions académiques, ainsi que les conséquences que peuvent avoir de telles actions, selon le Code de conduite de l'étudiant et des procédures disciplinaires. (pour de plus amples renseignements, veuillez consulter le site In accord with McGill University s Charter of Students Rights, students in this course have the right to submit in English or in French any written work that is to be graded. "Conformément à la Charte des droits de l étudiant de l Université McGill, chaque étudiant a le droit de soumettre en français ou en anglais tout travail écrit devant être noté (sauf dans le cas des cours dont l un des objets est la maîtrise d une langue)." Approved by Senate on 21 January "If you have a disability please contact the instructor to arrange a time to discuss your situation. It would be helpful if you contact the Office for Students with Disabilities at before you do this." "Guidelines for the use of mobile computing and communications (MC2) devices in classes at McGill have been approved by the APC. Consult the guidelines for a range of sample wording that may be used or adapted by instructors on their course outlines."

10 Instructor generated course materials (e.g., handouts, notes, summaries, exam questions, etc.) are protected by law and may not be copied or distributed in any form or in any medium without explicit permission of the instructor. Note that infringements of copyright can be subjected to follow up by the University under the Code of Student Conduct and Disciplinary Procedures. End-of-term course evaluations are one of the ways that McGill works towards maintaining and improving the quality of courses and the student s learning experience. You will be notified by e- mail when the evaluations are available on Mercury, the online course evaluation system. Please note that a minimum number of responses must be received for results to be available to students. "McGill has policies on sustainability, paper use and other initiatives to promote a culture of sustainability at McGill. (See the Office of Sustainability.) In keeping with McGill's preparedness planning strategies with respect to potential pandemic or other concerns, the Administration suggests that all course outlines for the academic year contain the statement: In the event of extraordinary circumstances beyond the University s control, the content and/or evaluation scheme in this course is subject to change. "Additional policies governing academic issues which affect students can be found in the McGill Charter of Students' Rights (The Handbook on Student Rights and Responsibilities is available at