Degree in Biotechnology. Genetically Modified Organisms. Type: Compulsory Credits ECTS: 6. Applied Biotechnology

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1 IDENTIFICATION DETAILS Title: Degree in Biotechnology Faculty/School: Bio-Sanitary Sciences Course subject: Genetically Modified Organisms Type: Compulsory Credits ECTS: 6 Year / Semester: Year 4-7 th Semester Code: 2042 Subject: Applied Biotechnology Module: Biotechnological Processes and Products Language: Spanish Total number of hours undertaken 150 by pupil: DESCRIPTION OF THE COURSE The subject Genetically Modified Organisms, taught in the 7 th semester of the degree in Biotechnology, focuses on the study of several strategies generating genetically modified plant and animal organisms, in vitro or in vivo. It also focuses on their possible applications to problems of different biological nature. SKILLS TO DEVELOP 3.1. General skills To develop the necessary skills to search, learn, analyze, summarize and connect information. To be familiar with the principles and fundamentals in experimental and human sciences. To develop verbal and written communication skills. To develop the skills required in experimental work: design, performance, obtaining results and conclusions, understanding the limitations of the experimental approach. To be familiar with biotechnological applications in the health, food, agrobiotechnology, environmental, and chemical fields. 1 To develop leadership and team management skills.

2 To develop analytical, synthetical, reflective, critical, theoretical and practical thinking skills. To develop problem solving and decision making skills. To learn how to efficiently manage time. To be aware of the mutual influence between science, society and technological development in view of a sustainable future. To develop the ability and commitment of independent learning and personal development Specific skills To describe the most relevant biotechnological products in the development of new pharmaceutical drugs (vaccines, monoclonal antibodies, chemokines and cytokines, peptides and proteins, anti-sense oligonucleotids and interference RNA). To note the influence and contributions of new technologies in Molecular and Cell Biology to the pharmaceutical sector. To explain the use of biotechnological advances in agrobiotechnology to our society. To describe the biotechnological processes applied in the chemical and environmental industries. To be aware of the methods to obtain genetically modified organisms as the basis for animal testing and its relevance for pathology diagnosis and treatment. To be familiar with the requirements of microorganisms and cell lines regarding large-scale fermentation. To have a good knowledge of gene transfer in plants and its biotechnological application. To understand and know how to apply genetic and genomic technologies to the plant kingdom. To understand how the basic ideas developed in a laboratory in different models can become biotechnological applications at the service of society. To properly work in a laboratory handling biological material (bacteria, fungi, virus, animal and plant cells, plants and animals) including biological waste treatment, disposal and safety. To properly design and perform an experimental protocol based on the theoretical aspects of different matters. To identify and define laboratory equipment and material. To encourage intellectual curiosity and search of the truth in all life spheres. To develop rigorous thinking habits. To communicate the knowledge gained through proper verbal and written skills. To analyze and summarize the main ideas and content in all texts; to discover their thesis and issues raised, and critically judge their form and substance. To apply the theoretical knowledge gained to the resolution of problems and practical cases in different matters. To develop self-assessment skills. To work in teams in an effective and coordinated manner. 2

3 PREREQUISITES Students taking the subject Genetically Modified Organisms and willing to successfullly completed should have basic knowledge of Plant and Animal Physiology, Genetice Engineering and Cell Biology. WORK TIME DISTRIBUTION CONTACT HOURS REMOTE STUDY HOURS OBJECTIVES To know the different gene transformation systems used in plants. To understand the fundamentals of transgenic expression in plants. To understand transient transgenic expression in plants. To apply plant transgenic systems to generate products of interest. To know the basic concepts of animal cell culture (applications, advantages and disadvantages, sterile culture, equipment...). To know animal cell culture methods and their different elements such as instruments and saline solutions, supports, To know the obtention and maintenance methods of established primary cultures and cell lines. To analyze relevant bioprocesses in animal cell cultures, such as cell division, viability or cytotoxicity. To apply uses of animal cell cultures to protein generation, monoclonal antibodies, etc. To distinguish stem cell types and proper culture conditions. Applications of the use of tridimensional cultures to tissue engineering. Applications of animal cell culture to the design of bioreactors. To know in depth the basic biology of D. melanogaster. To apply the different genetic modification techniques to D. melanogaster obtention for its use as a lab tool. To know in depth the basic biology of M. musculus. To study embryo production in zygote phase and murine embryonic stem cells for their subsequent genetic modification. To know the different genome modification strategies of M. musculus for its use as a lab tool. INDEX OF SUBJECTS First Part: Plants. Unit 1: Introduction to plant transgenesis. Unit 2: Plant transformation systems. Unit 3: Transgenic expression. Unit 4: Introduction plant transgenesis applications. Unit 5: Transient transgenic expression. Second Part: Animals (I) Cell culture Unit 6: Introduction to cell cultures. Unit 7: Animal cell culture methods. Unit 8: Production and maintenance of primary cultures and cell lines for in vitro development. Unit 9: Bioprocess analysis in animal cells. Unit 10: Specialized techniques. Unit 11: Stem cells. Unit 12: Tridimensional cell cultures. Unit 13: Bioreactors. 3

4 Third Part: Animals (II). Unit 14: Introduction to the generation of genetically modified organisms. Unit 15: D. melanogaster. Unit 16: Introduction to M. musculus. Unit 17: M. musculus transgenic models. Unit 18: M. musculus models generated by homologous recombination (I). Classic models. Unit 19: M. musculus models generated by homologous recombination (II). Classic models. METHODOLOGY/LEARNING ACTIVITIES This subject will use different methodologies with the aim of achieving the objectives proposed. Face-toface hours will be distributed in master classes given by subject professors or invited ones, and the discussion of scientific articles and/or works developed by students during contact hours. EVALUATION SYSTEM The final grade of the subject depends on several parts, being the assessment of master classes an eseential one. Also, the assessment of group assignments and discussion of scientific articles will be considered. BIBLIOGRAPHY Culture of Animal Cells. A manual of basic technique. R. Ian Freshney. Ed Ed. Wiley Animal Cell Culture. John R. W. Masters. Ed Ed. Oxford University Press Técnicas en Histología y Biología Celular. Luis Montuenga y col. 2009, Elsevier Masson. Cultivo de Células Animales y Humanas. Aplicaciones en Medicina Regenerativa. Pablo E. Gil- Loyzaga Vision Libros Handbook of industrial cell culture. V.A. Vinci and S.R. Parekh. Human Press Transgenesis Techniques. Edited by Alan R. Clarke. Methods in Molecular Biology (vol.180). Ed. Humana Press. Drosophila. Methods and Protocols. Edited by C. Dahmann. Serie: Methods in Molecular Biology. Ed. Humanas Press. Mouse Genetics and Transgenics. Edited by I.J. Jackson and C.M. Abbot. Serie A Practical Approach. Ed. Oxford University Press. Manual de genética de roedores de laboratorio: Principios básicos y aplicaciones. Fernando J. Benavides y Jean-Louis Guénet. Ed. Universidad de Alcalá y Sociedad española para las ciencias del animal de laboratorio. Gene Targeting. Edited by A.L. Joyner. Serie A Practical Approach. Ed. Oxford University Press. Genes IX. Benjamin Lewin. Ed. Oxford. Genética Molecular Humana. Tom Strachan, Andrew P. Read. Ed. Omega. Debido al carácter actual de la asignatura la mayor parte de la información de esta asignatura provendrá de la lectura y comentario crítico de artículos publicados en revistas científicas. 4

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