M.Sc Immunology & Microbiology. B.Sc Medical Laboratory Technology

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1 Name: Farah M. Khalifeh Tel: Education: American University of Beirut (Beirut- Lebanon) M.Sc Immunology & Microbiology University of Jordan (Amman- Jordan) B.Sc Medical Laboratory Technology Experience: 2012-Present Stem Cells of Arabia (Amman, Jordan) Research & Development Researcher Med Labs Consultancy Group (Amman, Jordan) Medical Laboratory Technologist American University of Beirut (Beirut, Lebanon) Graduate Assistant

2 Skills: Knowledge in Research methodology: strategies, planning, and analysis Knowledge of validity, effect measure and choice of statistical test Writing experimental protocols Knowledge of pilot studies and controlled clinical trials, estimating sample size, Non-inferiority studies Measures of disease occurrence, association, risk and implications Study designs of epidemiological studies, effect measures in epidemiological studies Experimental studies and randomized control trials, measurement error and sources of error, qualitative data collection, triangulation, analysis of qualitative data, hypothesis testing and confidence interval, comparison of means, regression analysis, normal distribution Excellent scientific and medical writing skills Reviewing and editing manuscripts, laboratory documentation, results, and reports Development of standard operating procedures (SOPs) for laboratory techniques and maintenance of quality control follow up procedures Knowledge in using Genetyx, RNA structure, and Primer3 Software Knowledge in using SPSS and ANOVA statistics software Excellent skills in using referencing and citation software (Endnote, Mendeley)

3 Stem cell biology, human stem cell culture (adipose, bone marrow, cord blood), mouse stem cell culture, real-time PCR, immunocytochemistry, flow cytometry, FACS, tumor induction in mice, microscopy, cellular oxygen consumption measurements, ELISA Freezing & Thawing of cells Magnetic cell sorting (MACS) for separation of hematopoietic stem cells (CliniMACS, AutoMACS) DNA and RNA extractions, standard PCR reactions, realtime PCR reactions, restriction enzyme digestion, reagents preparation, primer design, ligations, and recombinant protein purification Recombinant DNA technology Bioinformatics Protein Analysis Western, Southern, and Northern blotting Mammalian cell and tissue culture including media change, passage, long term storage Lymphocyte isolation (Ficoll and Percoll density gradient centrifugation) Fluorescent In Situ Hybridization (FISH) Microbiology: Liquid and solid culture of bacteria, isolation and transformation, preparation of competent cells, long term storage of bacteria, and DNA sequencing Lyophilization techniques Agarose Gel Electrophoresis, operating UV gel detection system and saving images, interpretation of gel results SDS-PAGE Antibody Purification Protein Purification Semen Analysis (volume, liquefaction time, sperm count, morphology, motility ph, WBC count, fructose level)

4 Semen analysis for infertile subjects (DGC, swim-up technique, percoll separation, vital stain, IL-6 analysis, and FISH) Differentiation of multiple cell types in semen specimens and testicular biopsies Languages: Fluent in English and Arabic languages Accomplishments: 1. Master's Thesis project entitled: "The Effect of the Supernatant of Coley's Mixed Bacterial Toxin (MBT) and Bacterial Lipopolysaccharide (LPS) on Serum Levels of TNF-α, IL-12, and VEGF in Mice." Nayla S. Al-Akl, Farah Khaliefeh, Alexander M. Abdelnoor IOSR Journal of Pharmacy Mar.-Apr. 2012, Vol. 2(2) pp: "Effects of catalase and 1400W on the number of interleukin-4 and interferon-γ secreting spleen cells in mice injected with ovalbumin and alum." Al-Akl NS, Ismail M, Khaliefeh F, Usta J, Abdelnoor AM Immunopharmacol Immunotoxicol Dec; 34(6): "The effectiveness of stem cell therapies on health-related quality of life and life expectancy in comparison with conventional supportive medical treatment in patients suffering from end-stage liver disease." Alzoubi AM, Khalifeh F. Stem Cell Res Ther Feb 25; 4(1):16

5 4. Transplantation of Purified Autologous Leukapheresis- DerivedCD34+ and CD133+ Stem Cells for Patients with Chronic Spinal Cord Injuries: Long-term Evaluation of Safety and Efficacy. Al-Zoubi A, Jafar E, Jamous M, Al-Twal F, Al-Bakheet S, Zalloum M, Khalifeh F, Radi SA, El-Khateeb M, Al-Zoubi Z. Cell Transplant. 2014; 23 Suppl 1:S doi: / X Epub 2014 Nov 4. - A speaker at the International Association of Neurorestratology (IANR) VI and 10 th global college of Neuroprotection & Neuroregeneration (GCNN) Annual Conference presenting a lecture entitled:"ex vivo differentiation of human bone marrow derived CD34+ and CD133+ stem cells into neuronal cell lineages" 4-7 April, 2013 Bucharest, Romania. - Participated in the 4 th International Jordanian Congress of Allergy & Immunology September 19-21, 2012 Amman, Jordan. - Participated in the 1 st Middle East Wound Care & Diabetic Limb Salvage (WDLS) Conference